A novel flow injection chemiluminescence （CL） method for the determination of ciprofloxacin （CPLX） was reported. The proposed method is based on luminescence produced by KMnO4-Na2S2O4-Tb^3＋-CPLX chemiluminescence...A novel flow injection chemiluminescence （CL） method for the determination of ciprofloxacin （CPLX） was reported. The proposed method is based on luminescence produced by KMnO4-Na2S2O4-Tb^3＋-CPLX chemiluminescence （CL） system. The effects of some critical experimental conditions were discussed and the optimization of working conditions was investigated. The linear dynamic range is 3.00 × 10^-8 - 9.00 × 10^-7 mol ·L-1 and the detection limit for CPLX is 4.40 × 10^-9 mol·L^-1. This method was applied to the determination of CPLX in tablet, urine, and serum samples and the recoveries of real sample analyses were in the range from 110 ± 4 to 104 ± 4.展开更多
A sensitive and selective method employing chemiluminescence(CL)coupled with flow injection(FI)is reported for nalbuphine hydrochloride(NAL)assay in pharmaceutical formulations.The enhancement effect of NAL on the CL ...A sensitive and selective method employing chemiluminescence(CL)coupled with flow injection(FI)is reported for nalbuphine hydrochloride(NAL)assay in pharmaceutical formulations.The enhancement effect of NAL on the CL reaction between tris(2,2′-bipyridyl)ruthenium(II)chloride-diperiodatocuprate(III){Ru[(bpy)_(3)]^(2+)-Cu(III)complex}in acidic medium is used as analytical measurement.The optimal conditions of the CL reaction were sulfuric acid 1.0×10^(−3) mol/L,Ru[(bpy)s]2+7.5×10^(−5) mol/L,Cu(III)/Ag(III)complexes 4.0×10^(−4)/5.0×10^(−4) mol/L,sample loop volume of 120µL and flow rate of 2.5 mL/min.The sensitivities of the method in terms of detection(S/N=3)and quantification(S/N=10)limits are 5×10^(−4) and 0.001 ppm(1 ppm=1 mg/L),respectively.The linear response of the instrument in the form of CL intensity with respect to NAL concentration is over the range 0.001-15.0 ppm(R^(2)=0.9999)with relative standard deviation from 0.8%to 3.2%and injection throughput of 120 injection/h.The applications of the method include the quantitative analysis of NAL in pharmaceutical injection samples.Variations and the average results of the proposed method are not significantly different from the results of a reported method by applying F-and paired student t-test.The most likely CL reaction mechanism is written in accordance with spectrophotometric and CL studies.展开更多
In this paper, a novel chemiluminescence (CL) method for the determination of tramadol has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite an...In this paper, a novel chemiluminescence (CL) method for the determination of tramadol has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic KMnO4. A mechanism for the CL reaction has been proposed on the basis of fluorescent and CL spectra. Under the optimized conditions, the proposed method allows the measurement of tramadol hydrochloride over the range of 0.04 - 4 ?g/mLwith a correlation coefficient of 0.9995 (n = 8) and a detection limit of 0.01 ?g/mL (3σ), and the relative standard deviation for 2.0 ?g/mL tramadol (n = 11) is 2.1%. The utility of this method was demonstrated by determining tramadol hydrochloride in tablets and injections.展开更多
文摘A novel flow injection chemiluminescence （CL） method for the determination of ciprofloxacin （CPLX） was reported. The proposed method is based on luminescence produced by KMnO4-Na2S2O4-Tb^3＋-CPLX chemiluminescence （CL） system. The effects of some critical experimental conditions were discussed and the optimization of working conditions was investigated. The linear dynamic range is 3.00 × 10^-8 - 9.00 × 10^-7 mol ·L-1 and the detection limit for CPLX is 4.40 × 10^-9 mol·L^-1. This method was applied to the determination of CPLX in tablet, urine, and serum samples and the recoveries of real sample analyses were in the range from 110 ± 4 to 104 ± 4.
文摘A sensitive and selective method employing chemiluminescence(CL)coupled with flow injection(FI)is reported for nalbuphine hydrochloride(NAL)assay in pharmaceutical formulations.The enhancement effect of NAL on the CL reaction between tris(2,2′-bipyridyl)ruthenium(II)chloride-diperiodatocuprate(III){Ru[(bpy)_(3)]^(2+)-Cu(III)complex}in acidic medium is used as analytical measurement.The optimal conditions of the CL reaction were sulfuric acid 1.0×10^(−3) mol/L,Ru[(bpy)s]2+7.5×10^(−5) mol/L,Cu(III)/Ag(III)complexes 4.0×10^(−4)/5.0×10^(−4) mol/L,sample loop volume of 120µL and flow rate of 2.5 mL/min.The sensitivities of the method in terms of detection(S/N=3)and quantification(S/N=10)limits are 5×10^(−4) and 0.001 ppm(1 ppm=1 mg/L),respectively.The linear response of the instrument in the form of CL intensity with respect to NAL concentration is over the range 0.001-15.0 ppm(R^(2)=0.9999)with relative standard deviation from 0.8%to 3.2%and injection throughput of 120 injection/h.The applications of the method include the quantitative analysis of NAL in pharmaceutical injection samples.Variations and the average results of the proposed method are not significantly different from the results of a reported method by applying F-and paired student t-test.The most likely CL reaction mechanism is written in accordance with spectrophotometric and CL studies.
文摘In this paper, a novel chemiluminescence (CL) method for the determination of tramadol has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic KMnO4. A mechanism for the CL reaction has been proposed on the basis of fluorescent and CL spectra. Under the optimized conditions, the proposed method allows the measurement of tramadol hydrochloride over the range of 0.04 - 4 ?g/mLwith a correlation coefficient of 0.9995 (n = 8) and a detection limit of 0.01 ?g/mL (3σ), and the relative standard deviation for 2.0 ?g/mL tramadol (n = 11) is 2.1%. The utility of this method was demonstrated by determining tramadol hydrochloride in tablets and injections.
