Microporous titanium carbide coating was successfully synthesized on medical grade titanium alloy by using sequential carburization.Changes in the surface morphology of titanium alloy occasioned by sequential carburiz...Microporous titanium carbide coating was successfully synthesized on medical grade titanium alloy by using sequential carburization.Changes in the surface morphology of titanium alloy occasioned by sequential carburization were characterized and the wettability characteristics were quantified.Furthermore,the dispersion forces were calculated and discussed.The results indicate that sequential carburization is an effective way to modify the wettability of titanium alloy.After the carburization the surface dispersion force of titanium alloy increased from 76.5×10^(-3)J·m^(-2) to 105.5×10^(-3) J·m^(-2),with an enhancement of 37.9 %.Meanwhile the contact angle of titanium alloy decreased from 83° to 71.5°,indicating a significant improvement of wettability,which is much closer to the optimal water contact angle for cell adhesion of 70°.展开更多
In this study, six bacterial strains were isolated from the sediment, probiotic fermentation products, and lake sediments, they were identified as Bacillus amyloliquefaciens using genetic evolution analysis, which wer...In this study, six bacterial strains were isolated from the sediment, probiotic fermentation products, and lake sediments, they were identified as Bacillus amyloliquefaciens using genetic evolution analysis, which were named B3, B4, B5, XD3, YF6, and YF8. The comparison of the antibacterial activity, hemolytic activity, and antibiotic sensitivity of six Bacillus amyloliquefaciens strains laid a foundation for the development and application of antimicrobial peptide products. A surface activity assay was used to determine the production of biosurfactants in six Bacillus amyloliquefaciens strains. With Staphylococcus aureus and Escherichia coli as indicator bacteria, their antibacterial activity was determined using the agar diffusion method;the same diffusion method was used to determine the antibiotic susceptibility of Bacillus amyloliquefaciens. The results showed that the six Bacillus amyloliquefaciens strains had obvious biosurface activity, and the bacteria inhibited Staphylococcus aureus and Escherichia coli, from strong to weak: YF8, XD3, B3, B4, YF6, and B5. Strain YF8 had the best broad-spectrum bacteriostatic effect, followed by strain XD3. All Bacillus amyloliquefaciens strains were susceptible to 16 common drugs, except for Bacillus amyloliquefaciens strain YF8, which was intermediate to neomycin. The study shows that Bacillus amyloliquefaciens and secondary metabolites have the ability to produce a variety of active peptides, exert a certain inhibitory effect on common pathogens, and have the potential to develop as animal probiotics.展开更多
Due to trauma and disease,bone defects endanger the healthy life of human beings.At present,the gold standard for bone defect repair is still autologous bone transplantation and allogeneic bone transplantation.However...Due to trauma and disease,bone defects endanger the healthy life of human beings.At present,the gold standard for bone defect repair is still autologous bone transplantation and allogeneic bone transplantation.However,its insufficient source,potential disease transmission and immune rejection limit its clinical application.Therefore,the development of bone repair materials plays an important role in promoting bone repair.As the interface between material and tissue,the surface of the material plays an important role in the reaction after implantation,which determines the effectiveness of defect repair treatment.With the development of surface engineering and technology,bone repair materials have developed from biological inertia to biological activity by endowing various biological functions by controlling the composition,topological morphology and structure of the material surface etc.The inspired biofunctionalisation of material surface includes the capacities of inducing osteogenesis,promoting angiogenesis,antibacterial,immune regulation etc.,as well as integration of postoperative repair and treatment.The authors review the biofunctionalisation of biomaterial surface and the inspired biological effects for bone repair,mainly including physical and chemical properties of material surface to regulate osteogenesis,and functional strategy of bone repair material surface.展开更多
The purpose of this research is to develop data-driven machine learning(ML)models capable of estimating the specific wear rate of ultra-high molecular weight polyethylene(UHMWPE)used in hip replacement implants.The re...The purpose of this research is to develop data-driven machine learning(ML)models capable of estimating the specific wear rate of ultra-high molecular weight polyethylene(UHMWPE)used in hip replacement implants.The results of the data-driven models are demonstrating a high level of consistency with the experimental findings acquired from the pin-on-disk(POD)trials.With a performance evaluation of 0.06 mean absolute error(MAE),0.17 Root Mean Square Error(RMSE),and 0.96 R^(2),the Random Forest Regression is found to be the best model.Another machine learning model,called Gradient Boosting Regression,is also found to possess satisfactory predictive perfor-mance by having an MAE of 0.09,RMSE of 0.24,and R^(2)of 0.96.According to the findings of a parametric analysis that made use of an ML model,the surface texture geometry has a substantial dependence on the wear behaviour of UHMWPE bearings that are used in hip replacement implants.This strategy has the potential to enhance experiment design and lessen the necessity for time-consuming POD trials for the purpose of assessing the wear of hip replacement implants.展开更多
Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferatio...Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferation remains unclear.This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts.CCM samples with different values of surface rouges were prepared by polishing.MC3T3-E1 mouse osteoblasts were used for cell culture experiments.Cell attachment,morphology,and the expression of actin stress fibres,vinculin,and distri-bution of yes-associated protein were analysed.Our results suggest that surface rough-ness does not significantly affect cell attachment and proliferation on CCM,unlike on titanium.Thus implies that other properties of CCM,such as physicochemical properties,may play a more substantial role in modulating cell behaviour.This study provides important insights into the design of CCM implants,suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.展开更多
1D magnetic nanomaterials with iron,with the special physical properties and biological behaviour,have been found to possess the great promising applications in many fields.In this review,the components,structure,phys...1D magnetic nanomaterials with iron,with the special physical properties and biological behaviour,have been found to possess the great promising applications in many fields.In this review,the components,structure,physicochemical properties,biocompatibility and in vitro and in vivo biomedical functions of magnetic nanowires(MNWs),nanorods(MNRs)with iron are summarised,especially their anisotropy shape and magnetism result in their many applications in biodetections and medical treatment fields.The potential future functions of these 1D magnetic nanomaterials compared to magnetic nanoparticles also is discussed by highlighting the possibility of integration with other metal-compositions or bio-compositions and with existing biotechnology as well as by point-ing out their specific properties.Current limitations in the property improvement and issues related with the outcome of the MNRs in the body are also summarised in order to address the remaining challenge for the extended biomedical functions of MNRs in the clinical application field.展开更多
Reduction of energy consumption and improvement of cruising speed are greatly necessary for underwater vehicles.Previously,regular riblets have been machined and the drag reduction has been verified;however,the riblet...Reduction of energy consumption and improvement of cruising speed are greatly necessary for underwater vehicles.Previously,regular riblets have been machined and the drag reduction has been verified;however,the riblet parameters are not adjusted like the denticles of sharkskin,which adapt quickly to the complex changing fluid flow.To achieve an improved drag reduction effect on the complicated shape surface,a simple,low-cost,and timesaving stretching approach was proposed to adjust the riblet parameters on the underwater vehicle surface by controllable deformation.Nature latex rubber membrane with regular micro-riblets was prepared as a stretching flexible film,and the spacing and height of the micro-riblets were adjusted by adaptive control of the stretching ratio.The circulating water channel experiment verified the effectiveness and feasibility of the selfadaptive drag reduction by the controllable deformation method.The results demonstrated that the drag reduction rate of the controllable deformation bionic fish skin was 4.26%compared with a smooth surface at 0.25 m/s with an angle of attack of 0°,which is better than any other angle.The controllable deformation bionic fish skin provides a feasible method for the drag reduction of complex surface adaptive underwater vehicles.展开更多
基金supported by the National Nature Science Foundation of China (Grant No.50535050)the Vital Foundational 973 Program of Chinafoundation of China Scholarship Council (Project 2007CB607605).
文摘Microporous titanium carbide coating was successfully synthesized on medical grade titanium alloy by using sequential carburization.Changes in the surface morphology of titanium alloy occasioned by sequential carburization were characterized and the wettability characteristics were quantified.Furthermore,the dispersion forces were calculated and discussed.The results indicate that sequential carburization is an effective way to modify the wettability of titanium alloy.After the carburization the surface dispersion force of titanium alloy increased from 76.5×10^(-3)J·m^(-2) to 105.5×10^(-3) J·m^(-2),with an enhancement of 37.9 %.Meanwhile the contact angle of titanium alloy decreased from 83° to 71.5°,indicating a significant improvement of wettability,which is much closer to the optimal water contact angle for cell adhesion of 70°.
基金2024 Foshan City Social Science Planning Project(2024-GJ037)2023 Education Research and Reform Project of the Online Open Course Alliance in the Guangdong-Hong Kong-Macao Greater Bay Area+3 种基金2023 Foshan University Innovation and Entrepreneurship Training Program for College Students2022 Basic and Applied Basic Research Foundation of Guangdong Province(2022A1515140052)2022 Self-Funded Science and Technology Innovation Project of Foshan City(220001005797)2022 Innovation Project of Guangdong Graduate Education(2022JGXM129,2022JGXM128)and 2023(2023ANLK-080)。
文摘In this study, six bacterial strains were isolated from the sediment, probiotic fermentation products, and lake sediments, they were identified as Bacillus amyloliquefaciens using genetic evolution analysis, which were named B3, B4, B5, XD3, YF6, and YF8. The comparison of the antibacterial activity, hemolytic activity, and antibiotic sensitivity of six Bacillus amyloliquefaciens strains laid a foundation for the development and application of antimicrobial peptide products. A surface activity assay was used to determine the production of biosurfactants in six Bacillus amyloliquefaciens strains. With Staphylococcus aureus and Escherichia coli as indicator bacteria, their antibacterial activity was determined using the agar diffusion method;the same diffusion method was used to determine the antibiotic susceptibility of Bacillus amyloliquefaciens. The results showed that the six Bacillus amyloliquefaciens strains had obvious biosurface activity, and the bacteria inhibited Staphylococcus aureus and Escherichia coli, from strong to weak: YF8, XD3, B3, B4, YF6, and B5. Strain YF8 had the best broad-spectrum bacteriostatic effect, followed by strain XD3. All Bacillus amyloliquefaciens strains were susceptible to 16 common drugs, except for Bacillus amyloliquefaciens strain YF8, which was intermediate to neomycin. The study shows that Bacillus amyloliquefaciens and secondary metabolites have the ability to produce a variety of active peptides, exert a certain inhibitory effect on common pathogens, and have the potential to develop as animal probiotics.
基金National Natural Science Foundation of China,Grant/Award Number:52071277Sichuan Science and Technology Program,Grant/Award Number:2022YFSY0044。
文摘Due to trauma and disease,bone defects endanger the healthy life of human beings.At present,the gold standard for bone defect repair is still autologous bone transplantation and allogeneic bone transplantation.However,its insufficient source,potential disease transmission and immune rejection limit its clinical application.Therefore,the development of bone repair materials plays an important role in promoting bone repair.As the interface between material and tissue,the surface of the material plays an important role in the reaction after implantation,which determines the effectiveness of defect repair treatment.With the development of surface engineering and technology,bone repair materials have developed from biological inertia to biological activity by endowing various biological functions by controlling the composition,topological morphology and structure of the material surface etc.The inspired biofunctionalisation of material surface includes the capacities of inducing osteogenesis,promoting angiogenesis,antibacterial,immune regulation etc.,as well as integration of postoperative repair and treatment.The authors review the biofunctionalisation of biomaterial surface and the inspired biological effects for bone repair,mainly including physical and chemical properties of material surface to regulate osteogenesis,and functional strategy of bone repair material surface.
文摘The purpose of this research is to develop data-driven machine learning(ML)models capable of estimating the specific wear rate of ultra-high molecular weight polyethylene(UHMWPE)used in hip replacement implants.The results of the data-driven models are demonstrating a high level of consistency with the experimental findings acquired from the pin-on-disk(POD)trials.With a performance evaluation of 0.06 mean absolute error(MAE),0.17 Root Mean Square Error(RMSE),and 0.96 R^(2),the Random Forest Regression is found to be the best model.Another machine learning model,called Gradient Boosting Regression,is also found to possess satisfactory predictive perfor-mance by having an MAE of 0.09,RMSE of 0.24,and R^(2)of 0.96.According to the findings of a parametric analysis that made use of an ML model,the surface texture geometry has a substantial dependence on the wear behaviour of UHMWPE bearings that are used in hip replacement implants.This strategy has the potential to enhance experiment design and lessen the necessity for time-consuming POD trials for the purpose of assessing the wear of hip replacement implants.
基金Japan Society for the Promotion of Science,Grant/Award Number:JP19K12798。
文摘Co-Cr-Mo ally(CCM)is commonly used for orthopaedic and dental implants due to its excellent mechanical properties and corrosion resistance.However,the influence of surface roughness on cell attachment and proliferation remains unclear.This study aimed to elucidate the impact of surface roughness of CCM on the attachment and proliferation of osteoblasts.CCM samples with different values of surface rouges were prepared by polishing.MC3T3-E1 mouse osteoblasts were used for cell culture experiments.Cell attachment,morphology,and the expression of actin stress fibres,vinculin,and distri-bution of yes-associated protein were analysed.Our results suggest that surface rough-ness does not significantly affect cell attachment and proliferation on CCM,unlike on titanium.Thus implies that other properties of CCM,such as physicochemical properties,may play a more substantial role in modulating cell behaviour.This study provides important insights into the design of CCM implants,suggesting that approaches beyond tuning surface roughness may be necessary to improve biocompatibility and osseointegration.
基金the National Natural Science Foundation of China(project No.52273139)Sichuan Science and Technology Project(No.2023NSFSC0639 and 2023NSFSC1000).
文摘1D magnetic nanomaterials with iron,with the special physical properties and biological behaviour,have been found to possess the great promising applications in many fields.In this review,the components,structure,physicochemical properties,biocompatibility and in vitro and in vivo biomedical functions of magnetic nanowires(MNWs),nanorods(MNRs)with iron are summarised,especially their anisotropy shape and magnetism result in their many applications in biodetections and medical treatment fields.The potential future functions of these 1D magnetic nanomaterials compared to magnetic nanoparticles also is discussed by highlighting the possibility of integration with other metal-compositions or bio-compositions and with existing biotechnology as well as by point-ing out their specific properties.Current limitations in the property improvement and issues related with the outcome of the MNRs in the body are also summarised in order to address the remaining challenge for the extended biomedical functions of MNRs in the clinical application field.
基金Grant/Award Numbers:51725501,51935001,51905022National Key R&D Program of China,Grant/Award Number:2019YFB1309702。
文摘Reduction of energy consumption and improvement of cruising speed are greatly necessary for underwater vehicles.Previously,regular riblets have been machined and the drag reduction has been verified;however,the riblet parameters are not adjusted like the denticles of sharkskin,which adapt quickly to the complex changing fluid flow.To achieve an improved drag reduction effect on the complicated shape surface,a simple,low-cost,and timesaving stretching approach was proposed to adjust the riblet parameters on the underwater vehicle surface by controllable deformation.Nature latex rubber membrane with regular micro-riblets was prepared as a stretching flexible film,and the spacing and height of the micro-riblets were adjusted by adaptive control of the stretching ratio.The circulating water channel experiment verified the effectiveness and feasibility of the selfadaptive drag reduction by the controllable deformation method.The results demonstrated that the drag reduction rate of the controllable deformation bionic fish skin was 4.26%compared with a smooth surface at 0.25 m/s with an angle of attack of 0°,which is better than any other angle.The controllable deformation bionic fish skin provides a feasible method for the drag reduction of complex surface adaptive underwater vehicles.
