Background:Baitouweng decoction is a classic prescription for treating chronic dysentery and mainly used to treat heat-toxic dysentery and is widely used in damp-heat ulcerative colitis(UC)in China.Methods:Meta-analys...Background:Baitouweng decoction is a classic prescription for treating chronic dysentery and mainly used to treat heat-toxic dysentery and is widely used in damp-heat ulcerative colitis(UC)in China.Methods:Meta-analysis and network pharmacology were used to examine the pharmacological properties of Baitouweng decoction in the treatment of UC.Additionally,the potential mechanisms of action were investigated.In the meta-analysis,studies were searched from databases up to March 2024.Data from the included studies were extracted.The results were quantified by calculating the standardized mean difference(SMD).Additionally,95%confidence intervals(CI)were used to assess the precision of the estimates.Results:It was found that 201 components of Baitouweng decoction,including Pulsatillae Radix(Baitouweng),Coptidis Rhizoma(Huanglian),Phellodendri Chinensis Cortex(Huangbo),Fraxini Cortex(Qinpi),and 106 intersecting targets of UC,were obtained from INPUT.PPI and enrichment analyses showed Baitouweng decoction might regulate inflammatory response to improve UC injury.Seventeen included studies were published between 2004 and 2024.The meta-analysis results suggested that Baitouweng decoction may help increase body weight,decrease DAI and CMDI,reduce colon length shortening associated with UC,lower the spleen index,and alleviate tissue damage in colitis.In addition,Baitouweng decoction could inhibit inflammatory response and repair intestinal barrier in UC model.The protective mechanism of Baitouweng decoction was consistent with the predicted targets of network pharmacology,which suggested the results were accurate.Conclusion:Baitouweng decoction could improve UC injury by regulating the inflammatory response,cell apoptosis,and body metabolism through the integration of network pharmacology and meta-analysis.Its protective mechanisms are related to anti-inflammation,regulation of intestinal flora,brain-gut peptides,and protection of the intestinal mucosal barrier,along with modulation of body metabolism,including SCFA,bile acids,and tryptophan metabolism.展开更多
BACKGROUND Esophageal carcinoma(EC)is one of the most prevalent cancers in human populations worldwide.Baitouweng decoction is one of the most important Chinese medicine formulas,with the potential to treat cancer.AIM...BACKGROUND Esophageal carcinoma(EC)is one of the most prevalent cancers in human populations worldwide.Baitouweng decoction is one of the most important Chinese medicine formulas,with the potential to treat cancer.AIM To investigate the role and mechanism of Baitouweng decoction on EC cells.METHODS Differentially expressed genes(DEGs)in EC tissues and normal tissues were screened by the cDNA microarray technique and by bioinformatics methods.The target genes of microRNAs were predicted based on the TargetScan database and verified by dual luciferase gene reporter assay.We used Baitouweng decoction to intervene EC cells,and detected the activity of EC9706 and KYSE150 cells by the MTT method.Cell cycle and apoptosis were measured by flow cytometry.The expression of BUB1 mRNA and miR-495-3p was measured by qRT-PCR.The protein levels of BUB1,STAT3,p-STAT3,CCNB1,CDK1,Bax,Caspase3,and Caspase9 were measured by Western blot analysis.The migration and invasion abilities of the cells were measured by wound-healing assay and Transwell invasion assay,respectively.RESULTS DEGs identified are involved in biological processes,signaling pathways,and network construction,which are mainly related to mitosis.BUB1 was the key hub gene,and it is also a target gene of miR-495-3p.Baitouweng decoction could upregulate miR-495-3p and inhibit BUB1 expression.In vitro experiments showed that Baitouweng decoction significantly inhibited the migration and invasion of EC cells and induced apoptosis and G2/M phase arrest.After treatment with Baitouweng decoction,the expression of Bax,Caspase 3,and Caspase 9 in EC cells increased significantly,while the expression of BUB1,CCNB1,and CDK1 decreased significantly.Moreover,the STAT3 signaling pathway may play an important role in this process.CONCLUSION Baitouweng decoction has a significant inhibitory effect on EC cell growth.BUB1 is a potential therapeutic target for EC.Further analysis showed that Baitouweng decoction may inhibit the growth of EC cells by upregulating miR-495-3p targeting the BUB1-mediated STAT3 signal pathway.展开更多
基金supported by the National Natural Science Foundation of China(No.82405237,82373835,82173781)Guangdong Basic and Applied Basic Research Foundation(No.2023A1515110768,2019A1515010806)+3 种基金Foshan Science and Technology Bureau’s self funded project(No.2320001007283)Key Field Projects(Intelligent Manufacturing)of General Universities in Guangdong Province(No.2020ZDZX2057)the Scientific Research Projects(Characteristic Innovation)of General Universities in Guangdong Province(No.2019KTSCX195)Guangdong Provincial Medical Research Foundation(No.A2022061).
文摘Background:Baitouweng decoction is a classic prescription for treating chronic dysentery and mainly used to treat heat-toxic dysentery and is widely used in damp-heat ulcerative colitis(UC)in China.Methods:Meta-analysis and network pharmacology were used to examine the pharmacological properties of Baitouweng decoction in the treatment of UC.Additionally,the potential mechanisms of action were investigated.In the meta-analysis,studies were searched from databases up to March 2024.Data from the included studies were extracted.The results were quantified by calculating the standardized mean difference(SMD).Additionally,95%confidence intervals(CI)were used to assess the precision of the estimates.Results:It was found that 201 components of Baitouweng decoction,including Pulsatillae Radix(Baitouweng),Coptidis Rhizoma(Huanglian),Phellodendri Chinensis Cortex(Huangbo),Fraxini Cortex(Qinpi),and 106 intersecting targets of UC,were obtained from INPUT.PPI and enrichment analyses showed Baitouweng decoction might regulate inflammatory response to improve UC injury.Seventeen included studies were published between 2004 and 2024.The meta-analysis results suggested that Baitouweng decoction may help increase body weight,decrease DAI and CMDI,reduce colon length shortening associated with UC,lower the spleen index,and alleviate tissue damage in colitis.In addition,Baitouweng decoction could inhibit inflammatory response and repair intestinal barrier in UC model.The protective mechanism of Baitouweng decoction was consistent with the predicted targets of network pharmacology,which suggested the results were accurate.Conclusion:Baitouweng decoction could improve UC injury by regulating the inflammatory response,cell apoptosis,and body metabolism through the integration of network pharmacology and meta-analysis.Its protective mechanisms are related to anti-inflammation,regulation of intestinal flora,brain-gut peptides,and protection of the intestinal mucosal barrier,along with modulation of body metabolism,including SCFA,bile acids,and tryptophan metabolism.
基金Supported by National Natural Science Foundation of China,No.81550014Henan Provincial Science and Technology Research Project,No.222102310187 and No.242102310582Henan Province Postdoctoral Research Project,No.202103092.
文摘BACKGROUND Esophageal carcinoma(EC)is one of the most prevalent cancers in human populations worldwide.Baitouweng decoction is one of the most important Chinese medicine formulas,with the potential to treat cancer.AIM To investigate the role and mechanism of Baitouweng decoction on EC cells.METHODS Differentially expressed genes(DEGs)in EC tissues and normal tissues were screened by the cDNA microarray technique and by bioinformatics methods.The target genes of microRNAs were predicted based on the TargetScan database and verified by dual luciferase gene reporter assay.We used Baitouweng decoction to intervene EC cells,and detected the activity of EC9706 and KYSE150 cells by the MTT method.Cell cycle and apoptosis were measured by flow cytometry.The expression of BUB1 mRNA and miR-495-3p was measured by qRT-PCR.The protein levels of BUB1,STAT3,p-STAT3,CCNB1,CDK1,Bax,Caspase3,and Caspase9 were measured by Western blot analysis.The migration and invasion abilities of the cells were measured by wound-healing assay and Transwell invasion assay,respectively.RESULTS DEGs identified are involved in biological processes,signaling pathways,and network construction,which are mainly related to mitosis.BUB1 was the key hub gene,and it is also a target gene of miR-495-3p.Baitouweng decoction could upregulate miR-495-3p and inhibit BUB1 expression.In vitro experiments showed that Baitouweng decoction significantly inhibited the migration and invasion of EC cells and induced apoptosis and G2/M phase arrest.After treatment with Baitouweng decoction,the expression of Bax,Caspase 3,and Caspase 9 in EC cells increased significantly,while the expression of BUB1,CCNB1,and CDK1 decreased significantly.Moreover,the STAT3 signaling pathway may play an important role in this process.CONCLUSION Baitouweng decoction has a significant inhibitory effect on EC cell growth.BUB1 is a potential therapeutic target for EC.Further analysis showed that Baitouweng decoction may inhibit the growth of EC cells by upregulating miR-495-3p targeting the BUB1-mediated STAT3 signal pathway.
