AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control...AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.展开更多
Introduction: Following the COVID-19 pandemic, vaccination has been proposed in several countries as the main preventive measure despite very limited data, particularly in dialysis patients. We conducted this study to...Introduction: Following the COVID-19 pandemic, vaccination has been proposed in several countries as the main preventive measure despite very limited data, particularly in dialysis patients. We conducted this study to assess the immunological response to vaccination in Senegalese hemodialysis patients. Patients and Methods: We conducted a prospective study, in two dialysis centers in Dakar from March 30<sup>th</sup> to August 30<sup>th</sup>, 2021 including patients on hemodialysis for >6 months, vaccinated against SARS-CoV-2 according to the vaccination schedule recommended by WHO. A vaccine response was considered positive when seroconversion was observed after one dose of vaccine. The clinical efficacy of immunization was defined as the absence of new COVID-19 infection in patients who received a complete vaccination. Results: Among the 81 patients included in the study, 7.4% had anti-Spike IgM antibodies before their first vaccination. Seroprevalence of IgM antibodies was 38.3% one month after the first vaccine dose (at M1) and 8.6% one month after the second dose (at M4). Anti-Spike IgG antibodies were present in 40.3% of patients before vaccination, in 90.1% at M1, and in 59.7% at M4. Among patients previously infected with SARS-CoV-2, 10.2% had IgM antibodies at M0, 31.6% at M1, and 10.5% at M4 post-vaccination. Similarly, seroprevalences of IgG antibodies in this subgroup were 31.5%, 61.3%, and 50.0% respectively at M0, M1, and M4 post-vaccination. A comparison of seroconversion rates between M0 and M4 showed significant differences only for IgG in COVID-19 naive patients. Mean duration in dialysis and the existence of previous COVID-19 infection were associated with patients’ vaccinal response after the two doses. Age, gender and the use of immunosuppressive treatment did not influence post-vaccinal antibody production. Conclusion: Vaccination against COVID-19 in Senegalese hemodialysis patients induced a low seroconversion rate but it was well tolerated. Moreover, the induced protection was neither strong nor durable, particularly in patients with longer duration in dialysis.展开更多
[Objective]The research aimed to select the buckwheat accessions with higher Pb2＋ tolerance.[Method]Using 19 buckwheat accessions as materials,the Pb2＋ tolerance were studied by means of solution culture method.The ...[Objective]The research aimed to select the buckwheat accessions with higher Pb2＋ tolerance.[Method]Using 19 buckwheat accessions as materials,the Pb2＋ tolerance were studied by means of solution culture method.The length of root and the activity of root were used to evaluate the degree of Pb2＋ tolerance.[Result]Root growth and activity of root of T352,L2081,T308 and T393 were promoted under the low concentration of Pb2＋.However,with the concentration of Pb2＋ increasing,their root growth and activity decreased,indicating that these genotypes have the higher Pb2＋ tolerance.There were great variations of Pb2＋ tolerance of buckwheat in different areas.[Conclusion]There are different Pb2＋ tolerance in buckwheat,T352,T308,T442 and T393 which were originated from Weining had much higher Pb2＋ tolerance than the others,which can be used for researching on the buckwheat breeding and the mechanism of the buckwheat Pb2＋ tolerance.展开更多
Four rice ( Oryza sativa L.) cultivars 'IR72', 'Tesanai 2', 'Guichao 2' and 'IIyou 4480' were grown in two plastic house (15 m×3 m) with 35 μmol/mol and 60 μmol/mol CO 2 conc...Four rice ( Oryza sativa L.) cultivars 'IR72', 'Tesanai 2', 'Guichao 2' and 'IIyou 4480' were grown in two plastic house (15 m×3 m) with 35 μmol/mol and 60 μmol/mol CO 2 concentration which was controlled by computer. As compared with rice at ambient 35 μmol/mol CO 2, the changes in photosynthetic rate at elevated CO 2 showed up_regulation ('IR72' and 'Tesanai 2'), stable (unchanged) in 'Guichao 2' and down_regulation type ('IIyou 4480'). Growth rate, panicle weight, integrated water use efficiency (WUE) calculated from Δ 13 C and the capacity of scavenging DPPH · (1,1_diphenyl_2_picrylhydrazyl) free radical were increased at elevated CO 2. An increment in total biomass was observed in three cultivars by elevated CO 2, with the exception of 'IIyou 4480'. Ratios of panicle weight/total biomass were altered to different extents in tested cultivars by elevated CO 2. When leaf segments were subjected to PEG osmotic stress, the electrolyte leakage rate from leaves grown at elevated CO 2 was less than that at 35 μmol/mol CO 2. Those intraspecific variations of rice imply a possibility for selecting cultivars with maximal productivity and high tolerance to stresses adapted to elevated CO 2 in the future.展开更多
AIM: To investigate the signaling mechanism of antioxidative action by curcumin and its impact on glucose disposal. METHODS: Male C57BL/6J mice were fed with either a normal diet (n = 10) or a high fat diet (HFD) (n =...AIM: To investigate the signaling mechanism of antioxidative action by curcumin and its impact on glucose disposal. METHODS: Male C57BL/6J mice were fed with either a normal diet (n = 10) or a high fat diet (HFD) (n = 20) to induce obesity and insulin resistance. After 16 wk, 10 HFD-fed mice were further treated with daily curcumin oral gavage at the dose of 50 mg/kg body weight (BW) (HFD + curcumin group). After 15 d of the curcumin supplementation, an intraperitoneal glucose tolerance test was performed. Fasting blood samples were also collected for insulin and glucose measurements. Insulin-sensitive tissues, including muscle, adipose tissue and the liver, were isolated for the assessments of malondialdehyde (MDA), reactive oxygen species (ROS)and nuclear factor erythroid-2-related factor-2 (Nrf2) signaling. RESULTS: We show here that in a HFD mouse model, short-term curcumin gavage attenuated glucose intolerance without affecting HFD-induced BW gain. Curcumin also attenuated HFD-induced elevations of MDA and ROS in the skeletal muscle, particularly in its mitochondrial fraction, but it had no such an effect in either adipose tissue or the liver of HFD-fed mice. Correspondingly, in skeletal muscle, the levels of total or nuclear content of Nrf2, as well as its downstream target, heme oxygenase-1, were reduced by HFD-feeding. Curcumin intervention dramatically reversed these defects in Nrf2 signaling. Further analysis of the relationship of oxidative stress with glucose level by a regression analysis showed a positive and significant correlation between the area under the curve of a glucose tolerance test with MDA levels either in muscle or muscular mitochondria. CONCLUSION: These findings suggest that the shortterm treatment of curcumin in HFD-fed mice effectively ameliorates muscular oxidative stress by activating Nrf2 function that is a novel mechanism for its effect in improving glucose intolerance.展开更多
Fructokinase(FRK)is a regulator of fructose signaling in plants and gateway proteins that catalyze the initial step in fructose metabolism through phosphorylation.Our previous study demonstrated that MdFRK2 protein ex...Fructokinase(FRK)is a regulator of fructose signaling in plants and gateway proteins that catalyze the initial step in fructose metabolism through phosphorylation.Our previous study demonstrated that MdFRK2 protein exhibit not only high affinity for fructose,but also high enzymatic activity due to sorbitol.However,genome-wide identification of the MdFRK gene family and their evolutionary dynamics in apple are yet to be reported.A systematic genome-wide analysis in this study identified a total of nine MdFRK gene members,which could phylogenetically be clustered into seven groups.Chromosomal location and synteny analysis of MdFRKs revealed that their expansion in the apple genome is primarily driven by tandem and segmental duplication events.Divergent expression patterns of MdFRKs were observed in four source-sink tissues and at five different apple fruit developmental stages,which suggested their potential crucial roles in the apple fruit development and sugar accumulation.Reverse transcription-quantitative PCR(RT-qPCR)identified candidate NaCl or drought stress responsive MdFRKs,and transgenic apple plants overexpressing MdFRK2 exhibited considerably enhanced salinity tolerance.Our results will be useful for understanding the functions of MdFRKs in the regulation of apple fruit development and salt stress response.展开更多
We demonstrate a type of 2 × 2 multimode interference 3 dB coupler based on silicon-on-insulator.The fabrication tolerance was investigated by the effective index method and the guide mode method.The devices with...We demonstrate a type of 2 × 2 multimode interference 3 dB coupler based on silicon-on-insulator.The fabrication tolerance was investigated by the effective index method and the guide mode method.The devices with different lengths were fabricated and near-field output images were obtained.Tolerances to width,length and etch depth are 2,200 and 2μm,respectively.The devices show a uniform power distribution.展开更多
Semiconductor oxides are widely used to achieve photocatalytic removal of NOx(NO and NO2) species. These materials also exhibit enhanced oxidation ability in thermally assisted photocatalysis;however, many of them ten...Semiconductor oxides are widely used to achieve photocatalytic removal of NOx(NO and NO2) species. These materials also exhibit enhanced oxidation ability in thermally assisted photocatalysis;however, many of them tend to be deactivated at high relative humidity(RH) levels. In the case of the benchmark P25 TiO2 photocatalyst, we observe a significant decrease in non-NO2 selectivity from 95.02% to 58.33% when RH increases from 20% to 80%. Interestingly, the porous TiO2(B) microspheres synthesized in this work exhibit 99% selectivity at 20% RH;the selectivity remains as high as 96.18% at 80% RH. The high humidity tolerance of the TiO2(B) sample can be ascribed to its strong water desorption capacity and easy O2 adsorption at elevated temperatures, which reflects the fact that the superoxide radical is the main active species for the deep oxidation of NOx. This work may inspire the design of efficient photothermal catalysts with application in NOx removal in hot and humid environments.展开更多
基金Supported by the National Natural Science Foundation of China(No.81770898).
文摘AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.
文摘Introduction: Following the COVID-19 pandemic, vaccination has been proposed in several countries as the main preventive measure despite very limited data, particularly in dialysis patients. We conducted this study to assess the immunological response to vaccination in Senegalese hemodialysis patients. Patients and Methods: We conducted a prospective study, in two dialysis centers in Dakar from March 30<sup>th</sup> to August 30<sup>th</sup>, 2021 including patients on hemodialysis for >6 months, vaccinated against SARS-CoV-2 according to the vaccination schedule recommended by WHO. A vaccine response was considered positive when seroconversion was observed after one dose of vaccine. The clinical efficacy of immunization was defined as the absence of new COVID-19 infection in patients who received a complete vaccination. Results: Among the 81 patients included in the study, 7.4% had anti-Spike IgM antibodies before their first vaccination. Seroprevalence of IgM antibodies was 38.3% one month after the first vaccine dose (at M1) and 8.6% one month after the second dose (at M4). Anti-Spike IgG antibodies were present in 40.3% of patients before vaccination, in 90.1% at M1, and in 59.7% at M4. Among patients previously infected with SARS-CoV-2, 10.2% had IgM antibodies at M0, 31.6% at M1, and 10.5% at M4 post-vaccination. Similarly, seroprevalences of IgG antibodies in this subgroup were 31.5%, 61.3%, and 50.0% respectively at M0, M1, and M4 post-vaccination. A comparison of seroconversion rates between M0 and M4 showed significant differences only for IgG in COVID-19 naive patients. Mean duration in dialysis and the existence of previous COVID-19 infection were associated with patients’ vaccinal response after the two doses. Age, gender and the use of immunosuppressive treatment did not influence post-vaccinal antibody production. Conclusion: Vaccination against COVID-19 in Senegalese hemodialysis patients induced a low seroconversion rate but it was well tolerated. Moreover, the induced protection was neither strong nor durable, particularly in patients with longer duration in dialysis.
基金Supported by Science and Technology Foundation of Guizhou Province[Qiankehe-J(2009)No.2108)]Guizhou Normal UniversityFund for Doctor(2008)~~
文摘[Objective]The research aimed to select the buckwheat accessions with higher Pb2＋ tolerance.[Method]Using 19 buckwheat accessions as materials,the Pb2＋ tolerance were studied by means of solution culture method.The length of root and the activity of root were used to evaluate the degree of Pb2＋ tolerance.[Result]Root growth and activity of root of T352,L2081,T308 and T393 were promoted under the low concentration of Pb2＋.However,with the concentration of Pb2＋ increasing,their root growth and activity decreased,indicating that these genotypes have the higher Pb2＋ tolerance.There were great variations of Pb2＋ tolerance of buckwheat in different areas.[Conclusion]There are different Pb2＋ tolerance in buckwheat,T352,T308,T442 and T393 which were originated from Weining had much higher Pb2＋ tolerance than the others,which can be used for researching on the buckwheat breeding and the mechanism of the buckwheat Pb2＋ tolerance.
文摘Four rice ( Oryza sativa L.) cultivars 'IR72', 'Tesanai 2', 'Guichao 2' and 'IIyou 4480' were grown in two plastic house (15 m×3 m) with 35 μmol/mol and 60 μmol/mol CO 2 concentration which was controlled by computer. As compared with rice at ambient 35 μmol/mol CO 2, the changes in photosynthetic rate at elevated CO 2 showed up_regulation ('IR72' and 'Tesanai 2'), stable (unchanged) in 'Guichao 2' and down_regulation type ('IIyou 4480'). Growth rate, panicle weight, integrated water use efficiency (WUE) calculated from Δ 13 C and the capacity of scavenging DPPH · (1,1_diphenyl_2_picrylhydrazyl) free radical were increased at elevated CO 2. An increment in total biomass was observed in three cultivars by elevated CO 2, with the exception of 'IIyou 4480'. Ratios of panicle weight/total biomass were altered to different extents in tested cultivars by elevated CO 2. When leaf segments were subjected to PEG osmotic stress, the electrolyte leakage rate from leaves grown at elevated CO 2 was less than that at 35 μmol/mol CO 2. Those intraspecific variations of rice imply a possibility for selecting cultivars with maximal productivity and high tolerance to stresses adapted to elevated CO 2 in the future.
基金Supported by A National Natural Science Foundation of China Grant, No. 81072300 to Jin TR andYu ZW
文摘AIM: To investigate the signaling mechanism of antioxidative action by curcumin and its impact on glucose disposal. METHODS: Male C57BL/6J mice were fed with either a normal diet (n = 10) or a high fat diet (HFD) (n = 20) to induce obesity and insulin resistance. After 16 wk, 10 HFD-fed mice were further treated with daily curcumin oral gavage at the dose of 50 mg/kg body weight (BW) (HFD + curcumin group). After 15 d of the curcumin supplementation, an intraperitoneal glucose tolerance test was performed. Fasting blood samples were also collected for insulin and glucose measurements. Insulin-sensitive tissues, including muscle, adipose tissue and the liver, were isolated for the assessments of malondialdehyde (MDA), reactive oxygen species (ROS)and nuclear factor erythroid-2-related factor-2 (Nrf2) signaling. RESULTS: We show here that in a HFD mouse model, short-term curcumin gavage attenuated glucose intolerance without affecting HFD-induced BW gain. Curcumin also attenuated HFD-induced elevations of MDA and ROS in the skeletal muscle, particularly in its mitochondrial fraction, but it had no such an effect in either adipose tissue or the liver of HFD-fed mice. Correspondingly, in skeletal muscle, the levels of total or nuclear content of Nrf2, as well as its downstream target, heme oxygenase-1, were reduced by HFD-feeding. Curcumin intervention dramatically reversed these defects in Nrf2 signaling. Further analysis of the relationship of oxidative stress with glucose level by a regression analysis showed a positive and significant correlation between the area under the curve of a glucose tolerance test with MDA levels either in muscle or muscular mitochondria. CONCLUSION: These findings suggest that the shortterm treatment of curcumin in HFD-fed mice effectively ameliorates muscular oxidative stress by activating Nrf2 function that is a novel mechanism for its effect in improving glucose intolerance.
基金supported by the Yunnan Provincial Science and Technology Department Agriculture Joint Project,China(202301BD070001-020)。
文摘Fructokinase(FRK)is a regulator of fructose signaling in plants and gateway proteins that catalyze the initial step in fructose metabolism through phosphorylation.Our previous study demonstrated that MdFRK2 protein exhibit not only high affinity for fructose,but also high enzymatic activity due to sorbitol.However,genome-wide identification of the MdFRK gene family and their evolutionary dynamics in apple are yet to be reported.A systematic genome-wide analysis in this study identified a total of nine MdFRK gene members,which could phylogenetically be clustered into seven groups.Chromosomal location and synteny analysis of MdFRKs revealed that their expansion in the apple genome is primarily driven by tandem and segmental duplication events.Divergent expression patterns of MdFRKs were observed in four source-sink tissues and at five different apple fruit developmental stages,which suggested their potential crucial roles in the apple fruit development and sugar accumulation.Reverse transcription-quantitative PCR(RT-qPCR)identified candidate NaCl or drought stress responsive MdFRKs,and transgenic apple plants overexpressing MdFRK2 exhibited considerably enhanced salinity tolerance.Our results will be useful for understanding the functions of MdFRKs in the regulation of apple fruit development and salt stress response.
基金Supported by the National Natural Science Foundation of China under Grant Nos.69896260 and 69990540。
文摘We demonstrate a type of 2 × 2 multimode interference 3 dB coupler based on silicon-on-insulator.The fabrication tolerance was investigated by the effective index method and the guide mode method.The devices with different lengths were fabricated and near-field output images were obtained.Tolerances to width,length and etch depth are 2,200 and 2μm,respectively.The devices show a uniform power distribution.
文摘Semiconductor oxides are widely used to achieve photocatalytic removal of NOx(NO and NO2) species. These materials also exhibit enhanced oxidation ability in thermally assisted photocatalysis;however, many of them tend to be deactivated at high relative humidity(RH) levels. In the case of the benchmark P25 TiO2 photocatalyst, we observe a significant decrease in non-NO2 selectivity from 95.02% to 58.33% when RH increases from 20% to 80%. Interestingly, the porous TiO2(B) microspheres synthesized in this work exhibit 99% selectivity at 20% RH;the selectivity remains as high as 96.18% at 80% RH. The high humidity tolerance of the TiO2(B) sample can be ascribed to its strong water desorption capacity and easy O2 adsorption at elevated temperatures, which reflects the fact that the superoxide radical is the main active species for the deep oxidation of NOx. This work may inspire the design of efficient photothermal catalysts with application in NOx removal in hot and humid environments.
