目的:探讨微小RNA-296-5p、S期激酶相关蛋白2(S-phase kinase-associated protein 2,Skp2)、核糖体蛋白L6(ribosomal protein L6,RPL6)与前列腺癌病理特征的关系及对手术预后的影响。方法:选取2018年1月至2023年1月120例前列腺患者,均...目的:探讨微小RNA-296-5p、S期激酶相关蛋白2(S-phase kinase-associated protein 2,Skp2)、核糖体蛋白L6(ribosomal protein L6,RPL6)与前列腺癌病理特征的关系及对手术预后的影响。方法:选取2018年1月至2023年1月120例前列腺患者,均行腹腔镜前列腺癌术治疗,对比不同病理特征患者miR-296-5p、Skp2、RPL6 mRNA表达水平,分析各指标与病理特征的关系。根据术后1年是否生化复发分为复发组(n=21)与未复发组(n=97),比较2组临床资料、miR-296-5p、Skp2、RPL6mRNA表达水平,分析miR-296-5p、Skp2、RPL6对手术预后的影响及预测价值。结果:不同病理分期、Gleason评分、术前血清前列腺特异性抗原(prostate specific antigen,PSA)水平、淋巴结转移患者miR-296-5p、Skp2、RPL6 mRNA表达水平比较,差异有统计学意义(F=12.927、22.416、10.088、34.239、20.180、12.208,t=4.649、-5.770、-5.713、4.716、-5.647、-6.884,均P=0.000);miR-296-5p与Gleason评分、病理分期、术前血清前列腺特异性抗原(prostate specific antigen,PSA)水平、淋巴结转移呈负相关(r=-0.578、-0.539、-0.517、-0.556,均P<0.001),Skp2、RPL6 mRNA与Gleason评分、病理分期、术前血清PSA水平、淋巴结转移呈正相关(r=0.531、0.507、0.476、0.493、0.494、0.473、0.420、0.505,均P<0.001);复发组淋巴结转移、病理分期、切缘阳性率、Gleason评分、高于未复发组,术后辅助治疗率低于未复发组(P<0.05);复发组miR-296-5p表达水平低于未复发组,Skp2、RPL6mRNA表达水平高于未复发组(P<0.05);校正前,logistic回归分析,病理分期、Gleason评分、淋巴结转移、切缘阳性、术后辅助治疗、miR-296-5p、Skp2、RPL6 mRNA是前列腺癌患者术后生化复发的影响因素(P<0.05),校正后,miR-296-5p、Skp2、RPL6mRNA仍是前列腺癌患者术后生化复发的影响因素(P<0.05);miR-296-5p、Skp2、RPL6联合预测前列腺术后生化复发的AUC为0.902(95%CI=0.833~0.949),大于各指标单独预测(P<0.05)。结论:前列腺癌患者miR-296-5p、Skp2、RPL6表达水平与病理特征、术后复发密切相关,三者联合预测前列腺术后生化复发具有较高的参考价值。展开更多
Six new transition metal complexes, [Zn（HBTC）（PYTPY）]n·n PYTPY（1）, [Cu（HBTC）（PYTPY）]n·n PYTPY（2）, [Co（HBTC）（PYTPY）]n·n DMF（3）, [Mn（HBTC）（PYTPY）]n·n DMF（4）, [Cd（HBTC）（PYTP...Six new transition metal complexes, [Zn（HBTC）（PYTPY）]n·n PYTPY（1）, [Cu（HBTC）（PYTPY）]n·n PYTPY（2）, [Co（HBTC）（PYTPY）]n·n DMF（3）, [Mn（HBTC）（PYTPY）]n·n DMF（4）, [Cd（HBTC）（PYTPY）（H2O）]n·2nH2O（5）, and [Co（HBTC）（PYTPY）（H2O）2]（6）,（H3BTC = 1,3,5-benzenetricarboxylic acid, PYTPY = 4＇-（4-pyridyl）-2,2＇：6＇,2＇＇-terpyridine, DMF = N,N？-dimethylformamide）, have been synthesized and characterized by elemental analysis, IR and X-ray single-crystal diffraction. Complexes 1~5 all feature one-dimensional chain structures, and complex 6 exhibits a zero-dimensional structure. Complexes 1~5 present three-dimensional（3D） supramolecular frameworks via π-π stacking interactions, whenas 6 has also a 3D supramolecular structure assembled by hydrogen bonding. Meanwhile, complexes 1 ~ 6 exhibit the thermal stabilities and photoluminescent properties.展开更多
A compound ethyl(5S,6R)-2-(4-methylphenyl)-6-[(1R)-tert-butyldimethylsilyloxyethy]-penem-3-carboxylate was synthesized through displacement,acylation and Wittig cyclization reaction of optically active material(3R,4R)...A compound ethyl(5S,6R)-2-(4-methylphenyl)-6-[(1R)-tert-butyldimethylsilyloxyethy]-penem-3-carboxylate was synthesized through displacement,acylation and Wittig cyclization reaction of optically active material(3R,4R)-3-[(1R)-tert-butyldimethylsilyloxyethyl]-4-acetoxy-2-azetidi-none(4AA)upon thionocarboxlic acid.The intermediates and the target product were characterized by 1HNMR,IR,elementary analysis and MS.展开更多
The crystal structure of the title compound（C19H15F3N2O2,Mr = 360.33） was determined by single-crystal X-ray diffraction.The crystal belongs to triclinic,space group P1,with a = 6.5604（7）,b = 13.9614（16）,c = 18....The crystal structure of the title compound（C19H15F3N2O2,Mr = 360.33） was determined by single-crystal X-ray diffraction.The crystal belongs to triclinic,space group P1,with a = 6.5604（7）,b = 13.9614（16）,c = 18.1790（18） ,α = 102.749（7）,β = 97.542（6）,γ = 94.355（4）°,V = 1600.5（3） 3,Z = 4,Dc = 1.495 g/cm3,λ（MoKα） = 0.71070,F（000） = 744,μ（MoKα） = 0.122 mm-1,R = 0.0434 and wR = 0.1051.A total of 7590 unique reflections were collected,of which 5429 with ｜F｜2 ≥ 2σ｜F｜2 were observed.The two cyclohexene rings in the molecule adopt boat-boat conformations with the deviations of ring atoms C（9） and C10 from the C（5）/C（6）/C（7）/C（8） plane（Ⅰ） by 1.1204（0.0023） and 1.1132（0.0023） ,respectively,whereas from the C（2）/C（3）/C（5）/C（8） plane（Ⅱ） by 1.1627（0.0022） and 1.1818（0.0021） ,respectively.In the cyclopropane and lactam rings,atoms C（11） and N（1） point towards the double bond of C（9）-C（10） and the dihedral angle between the ring plane（Ⅲ） containing C（1）,C（2）,C（3） and C（4） and plane（IV） consisting of C（6）,C（7） and C（11） is 55.76（0.07）°.The dihedral angles between planes Ⅳ and Ⅰ and Ⅱ and Ⅲare 63.58（0.07）° and 58.10（0.06）°,respectively.The dihedral angle between the benzene ring C（13）～ C（18） and plane Ⅳ is 42.41（0.06）°.展开更多
文摘目的:探讨微小RNA-296-5p、S期激酶相关蛋白2(S-phase kinase-associated protein 2,Skp2)、核糖体蛋白L6(ribosomal protein L6,RPL6)与前列腺癌病理特征的关系及对手术预后的影响。方法:选取2018年1月至2023年1月120例前列腺患者,均行腹腔镜前列腺癌术治疗,对比不同病理特征患者miR-296-5p、Skp2、RPL6 mRNA表达水平,分析各指标与病理特征的关系。根据术后1年是否生化复发分为复发组(n=21)与未复发组(n=97),比较2组临床资料、miR-296-5p、Skp2、RPL6mRNA表达水平,分析miR-296-5p、Skp2、RPL6对手术预后的影响及预测价值。结果:不同病理分期、Gleason评分、术前血清前列腺特异性抗原(prostate specific antigen,PSA)水平、淋巴结转移患者miR-296-5p、Skp2、RPL6 mRNA表达水平比较,差异有统计学意义(F=12.927、22.416、10.088、34.239、20.180、12.208,t=4.649、-5.770、-5.713、4.716、-5.647、-6.884,均P=0.000);miR-296-5p与Gleason评分、病理分期、术前血清前列腺特异性抗原(prostate specific antigen,PSA)水平、淋巴结转移呈负相关(r=-0.578、-0.539、-0.517、-0.556,均P<0.001),Skp2、RPL6 mRNA与Gleason评分、病理分期、术前血清PSA水平、淋巴结转移呈正相关(r=0.531、0.507、0.476、0.493、0.494、0.473、0.420、0.505,均P<0.001);复发组淋巴结转移、病理分期、切缘阳性率、Gleason评分、高于未复发组,术后辅助治疗率低于未复发组(P<0.05);复发组miR-296-5p表达水平低于未复发组,Skp2、RPL6mRNA表达水平高于未复发组(P<0.05);校正前,logistic回归分析,病理分期、Gleason评分、淋巴结转移、切缘阳性、术后辅助治疗、miR-296-5p、Skp2、RPL6 mRNA是前列腺癌患者术后生化复发的影响因素(P<0.05),校正后,miR-296-5p、Skp2、RPL6mRNA仍是前列腺癌患者术后生化复发的影响因素(P<0.05);miR-296-5p、Skp2、RPL6联合预测前列腺术后生化复发的AUC为0.902(95%CI=0.833~0.949),大于各指标单独预测(P<0.05)。结论:前列腺癌患者miR-296-5p、Skp2、RPL6表达水平与病理特征、术后复发密切相关,三者联合预测前列腺术后生化复发具有较高的参考价值。
基金Supported by the National Natural Science Foundation of China(No.21576112)Natural Science Foundation of Jilin Province(20150623024TC-19,20170520147JH)the Science and Technology Development Plan of Siping City(2015049)
文摘Six new transition metal complexes, [Zn（HBTC）（PYTPY）]n·n PYTPY（1）, [Cu（HBTC）（PYTPY）]n·n PYTPY（2）, [Co（HBTC）（PYTPY）]n·n DMF（3）, [Mn（HBTC）（PYTPY）]n·n DMF（4）, [Cd（HBTC）（PYTPY）（H2O）]n·2nH2O（5）, and [Co（HBTC）（PYTPY）（H2O）2]（6）,（H3BTC = 1,3,5-benzenetricarboxylic acid, PYTPY = 4＇-（4-pyridyl）-2,2＇：6＇,2＇＇-terpyridine, DMF = N,N？-dimethylformamide）, have been synthesized and characterized by elemental analysis, IR and X-ray single-crystal diffraction. Complexes 1~5 all feature one-dimensional chain structures, and complex 6 exhibits a zero-dimensional structure. Complexes 1~5 present three-dimensional（3D） supramolecular frameworks via π-π stacking interactions, whenas 6 has also a 3D supramolecular structure assembled by hydrogen bonding. Meanwhile, complexes 1 ~ 6 exhibit the thermal stabilities and photoluminescent properties.
文摘A compound ethyl(5S,6R)-2-(4-methylphenyl)-6-[(1R)-tert-butyldimethylsilyloxyethy]-penem-3-carboxylate was synthesized through displacement,acylation and Wittig cyclization reaction of optically active material(3R,4R)-3-[(1R)-tert-butyldimethylsilyloxyethyl]-4-acetoxy-2-azetidi-none(4AA)upon thionocarboxlic acid.The intermediates and the target product were characterized by 1HNMR,IR,elementary analysis and MS.
文摘The crystal structure of the title compound（C19H15F3N2O2,Mr = 360.33） was determined by single-crystal X-ray diffraction.The crystal belongs to triclinic,space group P1,with a = 6.5604（7）,b = 13.9614（16）,c = 18.1790（18） ,α = 102.749（7）,β = 97.542（6）,γ = 94.355（4）°,V = 1600.5（3） 3,Z = 4,Dc = 1.495 g/cm3,λ（MoKα） = 0.71070,F（000） = 744,μ（MoKα） = 0.122 mm-1,R = 0.0434 and wR = 0.1051.A total of 7590 unique reflections were collected,of which 5429 with ｜F｜2 ≥ 2σ｜F｜2 were observed.The two cyclohexene rings in the molecule adopt boat-boat conformations with the deviations of ring atoms C（9） and C10 from the C（5）/C（6）/C（7）/C（8） plane（Ⅰ） by 1.1204（0.0023） and 1.1132（0.0023） ,respectively,whereas from the C（2）/C（3）/C（5）/C（8） plane（Ⅱ） by 1.1627（0.0022） and 1.1818（0.0021） ,respectively.In the cyclopropane and lactam rings,atoms C（11） and N（1） point towards the double bond of C（9）-C（10） and the dihedral angle between the ring plane（Ⅲ） containing C（1）,C（2）,C（3） and C（4） and plane（IV） consisting of C（6）,C（7） and C（11） is 55.76（0.07）°.The dihedral angles between planes Ⅳ and Ⅰ and Ⅱ and Ⅲare 63.58（0.07）° and 58.10（0.06）°,respectively.The dihedral angle between the benzene ring C（13）～ C（18） and plane Ⅳ is 42.41（0.06）°.
