钩端螺旋体病简称钩体病,是由不同类型的致病螺旋体感染所引起的急性全身感染性疾病。大部分病例无症状或轻度自限,而某些病例可以进展成重症甚至引起死亡^([1])。2022年6月我院儿童重症监护室(psychiatric intensive care unit,PICU)收...钩端螺旋体病简称钩体病,是由不同类型的致病螺旋体感染所引起的急性全身感染性疾病。大部分病例无症状或轻度自限,而某些病例可以进展成重症甚至引起死亡^([1])。2022年6月我院儿童重症监护室(psychiatric intensive care unit,PICU)收治1例重型钩体病合并肺出血、横纹肌溶解症、急性肾衰、脑膜炎、肝功、心功损害等多器官功能障碍的男性患儿,经抗感染、机械辅助通气、连续血液净化等综合治疗痊愈出院。现报告如下。展开更多
AIM： To evaluate the production of reactive oxygen species （ROS） and the expression of inducible nitric oxide synthase （iNOS） in rat isolated Kupffer cells （KCs） stimulated by Leptospira interrogans and Borreli...AIM： To evaluate the production of reactive oxygen species （ROS） and the expression of inducible nitric oxide synthase （iNOS） in rat isolated Kupffer cells （KCs） stimulated by Leptospira interrogans and Borrelia burgdorferi. METHODS： Rat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Pudfied Kupffer cells were tested in vitro with alive L.interogans and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies. RESULTS： B. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection. CONCLUSION： Both genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.展开更多
文摘钩端螺旋体病简称钩体病,是由不同类型的致病螺旋体感染所引起的急性全身感染性疾病。大部分病例无症状或轻度自限,而某些病例可以进展成重症甚至引起死亡^([1])。2022年6月我院儿童重症监护室(psychiatric intensive care unit,PICU)收治1例重型钩体病合并肺出血、横纹肌溶解症、急性肾衰、脑膜炎、肝功、心功损害等多器官功能障碍的男性患儿,经抗感染、机械辅助通气、连续血液净化等综合治疗痊愈出院。现报告如下。
文摘目的了解问号钩端螺旋体诱导不同宿主细胞凋亡的作用及相关胞内信号传导通路。方法建立问号钩体黄疸出血群赖型赖株小鼠单核-巨噬样细胞J774A．1、人脐静脉内皮细胞EVC304和人Ⅱ型肺泡上皮细胞A549感染模型。采用FITC-Annexin V／PI荧光标记流式细胞术检测细胞凋亡或坏死情况。分别采用荧光比色法和Western blot检测感染的J774A．1细胞caspase-3，-8，-9活性和凋亡相关蛋白FADD（Fas-associated death domain）表达水平。结果问号钩体赖株感染1-6h后，36．70％-63．70％的J774A．1细胞可出现明显的早期凋亡，感染12h时转变为晚期凋亡或坏死为主（53．68％）。78．52％问号钩体赖株感染的A549细胞仅出现晚期凋亡或坏死。问号钩体赖株感染的EVC304细胞无细胞凋亡或坏死现象。感染的J774A．1细胞caspase-3和-8最大活性分别为（1453．41±36．07）和（1402．15±59．09）FU，是未感染细胞的16．38和29．99倍。感染的J774A．1细胞caspase-9虽略有升高为（89．42±5．08）FU，但明显低于easpase-3和-8（P〈0．001）。随着感染时间的延长，感染的J774A．1细胞FADD蛋白表达量逐步增加。结论问号钩体诱导宿主细胞凋亡的效应可因细胞种类不同而有明显差异，FADD→caspase-8→caspase-3是介导问号钩体感染J774A．1细胞凋亡的主要信号通路。
文摘AIM： To evaluate the production of reactive oxygen species （ROS） and the expression of inducible nitric oxide synthase （iNOS） in rat isolated Kupffer cells （KCs） stimulated by Leptospira interrogans and Borrelia burgdorferi. METHODS： Rat Kupffer cells were separated by perfusion of the liver with 0.05% collagenase, and purified by Percoll gradients. Pudfied Kupffer cells were tested in vitro with alive L.interogans and B. burgdorferi preparations. The production of ROS was determined by chemiluminescence, whereas iNOS protein expression was evaluated by Western blot assay using anti-iNOS antibodies. RESULTS： B. burgdorferi and to a less extent L. interrogans induced ROS production with a peak 35 min after infection. The chemiluminescence signal progressively diminished and was undetectable by 180 min of incubation. Leptospirae and borreliae induced an increased iNOS expression in Kupffer cells that peaked at 6 hours and was still evident 22 h after infection. CONCLUSION： Both genera of spirochetes induced ROS and iNOS production in rat Kupffer cells. Since the cause of liver damage both in leptospiral as well as in borrelial infections are still unknown, we suggest that leptospira and borrelia damage of the liver can be initially mediated by oxygen radicals, and is then maintained at least in part by nitric oxide.
