目的探讨尼莫地平(nimodipin,NIM)对戊四氮(pentylenetetrazol,PTZ)点燃癫痫大鼠空间学习记忆能力及海马细胞内游离Ca2+浓度([Ca2+]i)、Ca2+/钙调蛋白依赖性蛋白激酶Ⅱα(calcium/calmodulin-dependent protein kinaseⅡα,CaMKⅡα)蛋...目的探讨尼莫地平(nimodipin,NIM)对戊四氮(pentylenetetrazol,PTZ)点燃癫痫大鼠空间学习记忆能力及海马细胞内游离Ca2+浓度([Ca2+]i)、Ca2+/钙调蛋白依赖性蛋白激酶Ⅱα(calcium/calmodulin-dependent protein kinaseⅡα,CaMKⅡα)蛋白表达的影响。方法将动物分为正常对照组、PTZ组和NIM+PTZ组,采用PTZ慢性点燃癫痫模型,应用Mor-ris水迷宫观察各组大鼠空间学习记忆能力,运用流式细胞仪检测海马细胞内[Ca2+]i变化,Western blot方法测定CaMKⅡα蛋白的表达。结果PTZ组大鼠空间学习记忆能力受损,其海马细胞内[Ca2+]i明显升高(P<0.05),CaMKⅡα蛋白水平较正常对照组减少(P<0.05);与PTZ组比较,NIM+PTZ组大鼠空间学习记忆能力好转,其海马细胞内[Ca2+]i下降(P<0.05),CaMKⅡα蛋白水平升高(P<0.05)。结论PTZ点燃癫痫大鼠海马存在钙超载及CaMKⅡα的表达异常,由此引起大鼠空间学习记忆能力受损;NIM可以降低细胞内[Ca2+]i,提高CaMKⅡα的表达,改善癫痫大鼠的学习记忆能力。展开更多
Ca 2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) is a member of a family of Ca 2+/calmodulin-regulated protein kinases which also includes Ca 2+/calmodulin-dependent protein kinases Ⅰ and Ⅲ, myosin light chain ...Ca 2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) is a member of a family of Ca 2+/calmodulin-regulated protein kinases which also includes Ca 2+/calmodulin-dependent protein kinases Ⅰ and Ⅲ, myosin light chain kinases and phosphorylase kinase. Unlike the other members of this family, CaMKⅡ is multifunctional protein kinase and distributes in a variety of tissues. It is especially abundant in neuronal system. In hippocampus, CaMKⅡ is about 2% of the total protein. Studies have shown that CaMKⅡ plays an important role in a variety of biological processes, such as regulation of gene transcription, synthesis of neurotransmitter, phosphorylation of cytoskeletonal protein, hippocampal learning and memory formation.展开更多
基金supported by High School Science and Technology Fund Planning Project of Tianjin Municipality,China(No.20060206)the Scientific Research Fund of Tianjin Medical University,China(No.2011ky33)
文摘目的探讨尼莫地平(nimodipin,NIM)对戊四氮(pentylenetetrazol,PTZ)点燃癫痫大鼠空间学习记忆能力及海马细胞内游离Ca2+浓度([Ca2+]i)、Ca2+/钙调蛋白依赖性蛋白激酶Ⅱα(calcium/calmodulin-dependent protein kinaseⅡα,CaMKⅡα)蛋白表达的影响。方法将动物分为正常对照组、PTZ组和NIM+PTZ组,采用PTZ慢性点燃癫痫模型,应用Mor-ris水迷宫观察各组大鼠空间学习记忆能力,运用流式细胞仪检测海马细胞内[Ca2+]i变化,Western blot方法测定CaMKⅡα蛋白的表达。结果PTZ组大鼠空间学习记忆能力受损,其海马细胞内[Ca2+]i明显升高(P<0.05),CaMKⅡα蛋白水平较正常对照组减少(P<0.05);与PTZ组比较,NIM+PTZ组大鼠空间学习记忆能力好转,其海马细胞内[Ca2+]i下降(P<0.05),CaMKⅡα蛋白水平升高(P<0.05)。结论PTZ点燃癫痫大鼠海马存在钙超载及CaMKⅡα的表达异常,由此引起大鼠空间学习记忆能力受损;NIM可以降低细胞内[Ca2+]i,提高CaMKⅡα的表达,改善癫痫大鼠的学习记忆能力。
文摘Ca 2+/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ) is a member of a family of Ca 2+/calmodulin-regulated protein kinases which also includes Ca 2+/calmodulin-dependent protein kinases Ⅰ and Ⅲ, myosin light chain kinases and phosphorylase kinase. Unlike the other members of this family, CaMKⅡ is multifunctional protein kinase and distributes in a variety of tissues. It is especially abundant in neuronal system. In hippocampus, CaMKⅡ is about 2% of the total protein. Studies have shown that CaMKⅡ plays an important role in a variety of biological processes, such as regulation of gene transcription, synthesis of neurotransmitter, phosphorylation of cytoskeletonal protein, hippocampal learning and memory formation.
