以三叶草根瘤菌野生型菌株Rhizobium trifolii ANU 843的寄主范围基因转移豌豆根瘤菌(Rhizobium leguminosarum)菌株300所获得的转移接合子290接种白三叶草。接种后24小时根毛细胞开始形成典型的弯曲;接种后48小时根毛细胞壁被部分溶解...以三叶草根瘤菌野生型菌株Rhizobium trifolii ANU 843的寄主范围基因转移豌豆根瘤菌(Rhizobium leguminosarum)菌株300所获得的转移接合子290接种白三叶草。接种后24小时根毛细胞开始形成典型的弯曲;接种后48小时根毛细胞壁被部分溶解,在被侵染的根毛细胞中可观察到侵染线,皮层细胞大量分裂。植物接种试验表明,白三叶草接种豌豆根瘤菌菌株300的不结瘤,而接种转移接合子290和菌株ANU 843的能结瘤,表明三叶草根瘤菌的寄主范围基因导入豌豆根瘤菌,改变了豌豆根瘤菌的寄主范围,因而能够诱导白三叶草正常结瘤。展开更多
Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation a...Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation amplitude,frequency on the viable cells of \R.leguminosarum\ L003 and oxygen transfer rate were investigated.It was found that enhanced oxygen transfer and biochemical reaction occurred in this system.Under the optimized conditions,about a 3\|fold increase of the viable cells was obtained in this system compared with that in a static tray fermenter.The overall oxygen transfer rate reached 487.01 mmol/(kg\5h)in this system in case of 0.35 MPa, t \-2=0.5 min against 37.46 mmol/(kg\5h) in static tray fermenter.展开更多
文摘Rhizobium leguminosarum\ L003,a kind of biofertilizers,was cultured in a periodic air pressure oscillating,solid\|state fermenter by using straw of wheat as an inert solid support.Effects of air pressure oscillation amplitude,frequency on the viable cells of \R.leguminosarum\ L003 and oxygen transfer rate were investigated.It was found that enhanced oxygen transfer and biochemical reaction occurred in this system.Under the optimized conditions,about a 3\|fold increase of the viable cells was obtained in this system compared with that in a static tray fermenter.The overall oxygen transfer rate reached 487.01 mmol/(kg\5h)in this system in case of 0.35 MPa, t \-2=0.5 min against 37.46 mmol/(kg\5h) in static tray fermenter.
