Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of lu...Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.展开更多
In micro electrical discharge machining （micro EDM）, it is difficult for servo controlling the narrow discharge gap with the characters of non-linear and quick change. In this paper, aiming at solving the problems a...In micro electrical discharge machining （micro EDM）, it is difficult for servo controlling the narrow discharge gap with the characters of non-linear and quick change. In this paper, aiming at solving the problems above, a self-adaptive fuzzy controller with formulary rule （SAFCFR） is presented based on the dual feedbacks composed by gap electric signal and discharge-ratio statistics. To ensure the properties of self-optimizing and fast stabilization, the formulary rule was designed with a tuning factor. In addition, the fast-convergence algorithms were introduced to adjust control target center and output scale factor. In this way, the normal discharge ratio can tend to the highest value during micro-EDM process. Experimental results show that the proposed algorithms are effective in improving the servo-control performance. According to the drilling-micro-EDM experiments, the machining efficiency is improved by 20% through applying SAFCFR. Moreover, SAFCFR is a prompt way to optimize parameters of discharge-gap servo control.展开更多
基金Project supported by the National 11th Five-Year Plan of Scientific and Technological Program (No. 2006BAI02B08) of ChinatheDepartment of Science and Technology of Zhejiang Province (No. 2003C33031), China
文摘Objective: To detect the effect of resistin on the transcription of insulin receptor promoter. Methods: Luciferase reporter gene was fused downstream of human insulin receptor promoter and the enzymatic activity of luciferase was determined in the presence or absence of resistin. The resistin expressed with plasmid was stained with antibody against Myc tag which was in frame fused with resistin coding sequence, and then imaged with confocal microscopy. Results: The treatment of pIRP-LUC transfected cells with recombinant resistin did not result in significant difference in the enzymatic activity of luciferase compared to the untreated cells. Cell staining showed that green fluorescence could be observed in the cytoplasm, but not in the nucleus. Conclusion: The results suggest that the endogenous resistin may functionally locate in the cytoplasm, but does not enter the nucleus and not down-regulate the transcription of insulin receptor promoter.
基金Supported by the National High Technology Research and Development Program of China （No. 2007AA04Z346） , the National Natural Science Foundation of China （ No. 50905094） and China Postdoctoral Science Foundation （ No. 20080440378, 200902097）.
文摘In micro electrical discharge machining （micro EDM）, it is difficult for servo controlling the narrow discharge gap with the characters of non-linear and quick change. In this paper, aiming at solving the problems above, a self-adaptive fuzzy controller with formulary rule （SAFCFR） is presented based on the dual feedbacks composed by gap electric signal and discharge-ratio statistics. To ensure the properties of self-optimizing and fast stabilization, the formulary rule was designed with a tuning factor. In addition, the fast-convergence algorithms were introduced to adjust control target center and output scale factor. In this way, the normal discharge ratio can tend to the highest value during micro-EDM process. Experimental results show that the proposed algorithms are effective in improving the servo-control performance. According to the drilling-micro-EDM experiments, the machining efficiency is improved by 20% through applying SAFCFR. Moreover, SAFCFR is a prompt way to optimize parameters of discharge-gap servo control.
