Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibratin...Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.展开更多
文摘Aim To determine in vitro the rat plasma protein binding rate by using microdialysis method.Methods The binding rate was determined by using microdialysis probe as sampling tools and zero-net flux method as calibrating method.The regression equation was made by the difference of concentrations between the dialysis sample and the perfusate.The（x-intercept） of regression equation was the free drug concentration（Cf）.The plasma protein binding rate was calculated by using the following equation: f=（C0-Cf）/C0.Result The binding rate was kept relatively stable in the studied concentration range.Conclusion It is feasible that the plasma protein binding rate can be determined by using microdialysis method.
基金National Science Foundation of China(81503236)Natural Science Foundation of Anhui Province,China(160808QH212+5 种基金1408085MH 1631308085MH126)Key Scientific Research Foundation of the Higher Education Institutions of Anhui ProvinceChina(KJ2015A1 55)Special Foundation for Talents of Yijishan Hospital(YR201407)Wannan Medical College Key Research Cultivation Project(WK2015ZF01)
