血脑屏障(blood brain barrier, BBB)是存在于外周血液和脑组织之间的结构,保护大脑免受内源性及外源性毒素和病原体的侵害,从而维持脑内环境稳定。在中枢神经系统疾病中,血脑屏障的破坏将进一步加剧疾病的进展。研究表明血小板源性生...血脑屏障(blood brain barrier, BBB)是存在于外周血液和脑组织之间的结构,保护大脑免受内源性及外源性毒素和病原体的侵害,从而维持脑内环境稳定。在中枢神经系统疾病中,血脑屏障的破坏将进一步加剧疾病的进展。研究表明血小板源性生长因子(platelet-derived growth factor, PDGF)在血脑屏障的形成及功能维持中发挥重要作用,本文总结了PDGF及其受体在生理及病理情况下对血脑屏障的影响,初步探讨PDGF在中枢神经系统疾病中的治疗可能性,并对当前靶向PDGF及其受体的治疗手段进行简要综述。The blood brain barrier (BBB) is a structure that exists between peripheral blood and brain tissue, protecting the brain from endogenous and exogenous toxins and pathogens, thereby maintaining the stability of the brain internal environment. In central nervous system diseases, the disruption of BBB further exacerbates disease progression. Compelling data indicates that platelet-derived growth factor (PDGF) plays an important role in the maintenance and formation of BBB. This article summarizes the effects of PDGFs and their receptors on the integrity of BBB under physiological and pathological conditions. Finally, we briefly summarize the potential therapeutic strategy of PDGFs in central nervous system diseases, and review the current therapeutic methods targeting PDGFR.展开更多
目的观察红芪多糖(HPS)对糖尿病大鼠视网膜血小板反应蛋白-1(TSP-1)和血小板源性生长因子-B(PDGF-B)表达的影响,探讨其对糖尿病视网膜病变的保护作用及可能机制。方法采用链脲佐菌素腹腔注射建立糖尿病模型。雄性Wistar大鼠随机分为模...目的观察红芪多糖(HPS)对糖尿病大鼠视网膜血小板反应蛋白-1(TSP-1)和血小板源性生长因子-B(PDGF-B)表达的影响,探讨其对糖尿病视网膜病变的保护作用及可能机制。方法采用链脲佐菌素腹腔注射建立糖尿病模型。雄性Wistar大鼠随机分为模型组、多贝斯组和HPS高、中、低剂量组,另设正常组,每组10只。各给药组给予相应药物灌胃,模型组和正常组给予等量生理盐水灌胃,1次/d,连续8周。q RT-PCR和免疫组化检测TSP-1和PDGF-B m RNA和蛋白表达;HE染色镜下观察视网膜的结构。结果模型组视网膜各层结构清晰、完整,但外核层疏松变薄、排列紊乱,神经节细胞数量稍减少;各给药组较模型组明显好转。与正常组比较,模型组视网膜TSP-1 m RNA和蛋白表达明显降低(P<0.01),PDGF-B m RNA和蛋白表达明显升高(P<0.01);与模型组比较,各给药组TSP-1 m RNA和蛋白表达明显升高(P<0.05,P<0.01),PDGF-B m RNA和蛋白表达明显降低(P<0.01);HPS高剂量组与其余给药组比较,TSP-1和PDGF-B m RNA和蛋白表达差异有统计学意义(P<0.05,P<0.01)。结论 HPS可能通过升高糖尿病大鼠视网膜组织TSP-1的表达和降低PDGF-B的表达来阻遏糖尿病视网膜病变进程中新生血管生成及增殖,从而起到保护视网膜的作用。展开更多
文摘血脑屏障(blood brain barrier, BBB)是存在于外周血液和脑组织之间的结构,保护大脑免受内源性及外源性毒素和病原体的侵害,从而维持脑内环境稳定。在中枢神经系统疾病中,血脑屏障的破坏将进一步加剧疾病的进展。研究表明血小板源性生长因子(platelet-derived growth factor, PDGF)在血脑屏障的形成及功能维持中发挥重要作用,本文总结了PDGF及其受体在生理及病理情况下对血脑屏障的影响,初步探讨PDGF在中枢神经系统疾病中的治疗可能性,并对当前靶向PDGF及其受体的治疗手段进行简要综述。The blood brain barrier (BBB) is a structure that exists between peripheral blood and brain tissue, protecting the brain from endogenous and exogenous toxins and pathogens, thereby maintaining the stability of the brain internal environment. In central nervous system diseases, the disruption of BBB further exacerbates disease progression. Compelling data indicates that platelet-derived growth factor (PDGF) plays an important role in the maintenance and formation of BBB. This article summarizes the effects of PDGFs and their receptors on the integrity of BBB under physiological and pathological conditions. Finally, we briefly summarize the potential therapeutic strategy of PDGFs in central nervous system diseases, and review the current therapeutic methods targeting PDGFR.
文摘目的观察红芪多糖(HPS)对糖尿病大鼠视网膜血小板反应蛋白-1(TSP-1)和血小板源性生长因子-B(PDGF-B)表达的影响,探讨其对糖尿病视网膜病变的保护作用及可能机制。方法采用链脲佐菌素腹腔注射建立糖尿病模型。雄性Wistar大鼠随机分为模型组、多贝斯组和HPS高、中、低剂量组,另设正常组,每组10只。各给药组给予相应药物灌胃,模型组和正常组给予等量生理盐水灌胃,1次/d,连续8周。q RT-PCR和免疫组化检测TSP-1和PDGF-B m RNA和蛋白表达;HE染色镜下观察视网膜的结构。结果模型组视网膜各层结构清晰、完整,但外核层疏松变薄、排列紊乱,神经节细胞数量稍减少;各给药组较模型组明显好转。与正常组比较,模型组视网膜TSP-1 m RNA和蛋白表达明显降低(P<0.01),PDGF-B m RNA和蛋白表达明显升高(P<0.01);与模型组比较,各给药组TSP-1 m RNA和蛋白表达明显升高(P<0.05,P<0.01),PDGF-B m RNA和蛋白表达明显降低(P<0.01);HPS高剂量组与其余给药组比较,TSP-1和PDGF-B m RNA和蛋白表达差异有统计学意义(P<0.05,P<0.01)。结论 HPS可能通过升高糖尿病大鼠视网膜组织TSP-1的表达和降低PDGF-B的表达来阻遏糖尿病视网膜病变进程中新生血管生成及增殖,从而起到保护视网膜的作用。
