目的:观察蒙药润僵汤对KOA模型小鼠膝关节组织形态学的影响,探讨其治疗膝骨关节炎的作用机理,为临床运用以及进一步探索蒙药润僵汤治疗膝骨关节炎的机制提供依据。方法:将40只C57BL/6小鼠随机区组法分为空白组、模型组、实验组(蒙药润僵...目的:观察蒙药润僵汤对KOA模型小鼠膝关节组织形态学的影响,探讨其治疗膝骨关节炎的作用机理,为临床运用以及进一步探索蒙药润僵汤治疗膝骨关节炎的机制提供依据。方法:将40只C57BL/6小鼠随机区组法分为空白组、模型组、实验组(蒙药润僵汤)、阳性对照组(中药海桐皮汤),每组10只。空白组膝关节腔内注射生理盐水处理,模型组、实验组、阳性对照组通过膝关节腔内注射胶原酶的方式建立KOA动物模型。术后1周开始用药,实验组以蒙药润僵汤浸泡治疗,阳性对照组以中药海桐皮汤浸泡治疗,空白组及模型组温水浸泡治疗。隔日1次,连续治疗2周。2周后行小动物膝关节MRI检查并处死动物后取股骨内髁关节软骨及部分软骨下骨标本,分别经HE染色。结果:小鼠膝关节MRI显示润僵汤组膝关节结构完整,关节腔内可见少量液体高信号影,所见肌肉组织信号正常。HE染色光镜下观察实验组大体同阳性对照组,关节软骨破坏较模型组明显轻;结论:蒙药润僵汤可能减少膝关节处炎症反应,减轻关节软骨破坏,减缓软骨下骨硬化,延缓骨关节炎的发展进程,但同时也表明其不能停止或逆转关节软骨的退变。Objective: To observe the effect of Mongolian medicine Runjiang Tang on the morphology of knee joint tissue in KOA model mice, explore its mechanism of action in treating knee osteoarthritis, and provide a basis for clinical application and further exploration of the mechanism of Mongolian medicine Runjiang Tang in treating knee osteoarthritis. Method: Forty C57BL/6 mice were randomly divided into a blank group, a model group, an experimental group (Mongolian medicine Runjiang Tang), and a positive control group (Chinese medicine Haitong Pi Tang), with 10 mice in each group. The blank group was treated with injecting physiological saline into the knee joint cavity, while the model group, experimental group, and positive control group were established with KOA animal models by injecting collagenase into the knee joint cavity. Medication started one week after surgery. The experimental group was treated with Mongolian medicine Runjiang Tang soaking, while the positive control group was treated with traditional Chinese medicine Haitongpi Tang soaking. The blank group and model group were treated with warm water soaking. Once every other day, continuous treatment for 2 weeks. Two weeks later, a small animal’s knee joint MRI examination was performed and the animal was euthanized. The femoral medial condylar joint cartilage and some subchondral bone specimens were taken and stained with HE, respectively. Result: MRI of the mouse knee joint showed that the knee joint structure of the Runjiang Tang group was intact, with a small amount of fluid high signal shadows visible in the joint cavity, and normal muscle tissue signals observed. Under the HE staining light microscope, the experimental group was generally the same as the positive control group, and the joint cartilage damage was significantly lighter than that of the model group. Conclusion: Mongolian medicine Runjiang Tang may reduce the inflammatory response at the knee joint, alleviate joint cartilage damage, slow down subchondral bone sclerosis, and delay the development process of osteoarthritis. However, it also indicates that it cannot stop or reverse the degeneration of joint cartilage.展开更多
文摘目的:观察蒙药润僵汤对KOA模型小鼠膝关节组织形态学的影响,探讨其治疗膝骨关节炎的作用机理,为临床运用以及进一步探索蒙药润僵汤治疗膝骨关节炎的机制提供依据。方法:将40只C57BL/6小鼠随机区组法分为空白组、模型组、实验组(蒙药润僵汤)、阳性对照组(中药海桐皮汤),每组10只。空白组膝关节腔内注射生理盐水处理,模型组、实验组、阳性对照组通过膝关节腔内注射胶原酶的方式建立KOA动物模型。术后1周开始用药,实验组以蒙药润僵汤浸泡治疗,阳性对照组以中药海桐皮汤浸泡治疗,空白组及模型组温水浸泡治疗。隔日1次,连续治疗2周。2周后行小动物膝关节MRI检查并处死动物后取股骨内髁关节软骨及部分软骨下骨标本,分别经HE染色。结果:小鼠膝关节MRI显示润僵汤组膝关节结构完整,关节腔内可见少量液体高信号影,所见肌肉组织信号正常。HE染色光镜下观察实验组大体同阳性对照组,关节软骨破坏较模型组明显轻;结论:蒙药润僵汤可能减少膝关节处炎症反应,减轻关节软骨破坏,减缓软骨下骨硬化,延缓骨关节炎的发展进程,但同时也表明其不能停止或逆转关节软骨的退变。Objective: To observe the effect of Mongolian medicine Runjiang Tang on the morphology of knee joint tissue in KOA model mice, explore its mechanism of action in treating knee osteoarthritis, and provide a basis for clinical application and further exploration of the mechanism of Mongolian medicine Runjiang Tang in treating knee osteoarthritis. Method: Forty C57BL/6 mice were randomly divided into a blank group, a model group, an experimental group (Mongolian medicine Runjiang Tang), and a positive control group (Chinese medicine Haitong Pi Tang), with 10 mice in each group. The blank group was treated with injecting physiological saline into the knee joint cavity, while the model group, experimental group, and positive control group were established with KOA animal models by injecting collagenase into the knee joint cavity. Medication started one week after surgery. The experimental group was treated with Mongolian medicine Runjiang Tang soaking, while the positive control group was treated with traditional Chinese medicine Haitongpi Tang soaking. The blank group and model group were treated with warm water soaking. Once every other day, continuous treatment for 2 weeks. Two weeks later, a small animal’s knee joint MRI examination was performed and the animal was euthanized. The femoral medial condylar joint cartilage and some subchondral bone specimens were taken and stained with HE, respectively. Result: MRI of the mouse knee joint showed that the knee joint structure of the Runjiang Tang group was intact, with a small amount of fluid high signal shadows visible in the joint cavity, and normal muscle tissue signals observed. Under the HE staining light microscope, the experimental group was generally the same as the positive control group, and the joint cartilage damage was significantly lighter than that of the model group. Conclusion: Mongolian medicine Runjiang Tang may reduce the inflammatory response at the knee joint, alleviate joint cartilage damage, slow down subchondral bone sclerosis, and delay the development process of osteoarthritis. However, it also indicates that it cannot stop or reverse the degeneration of joint cartilage.
