Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study...Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.展开更多
文摘【目的】探讨不同俯卧位联合反特伦德伦伯格卧位对急性呼吸窘迫综合征(Acute respiratory distress syndrome,ARDS)患者肺通气功能及呼吸力学的影响。【方法】选取2018年2月至2020年3月在商洛市中心医院行机械通气的96例ARDS患者,采用随机数字表法分为A组、B组、C组,A组采用俯卧位2 h后改为反特伦德伦伯格卧位通气2 h;B组采用俯卧位通气2 h后俯卧位联合反特伦德伦伯格卧位通气2 h;C组采用俯卧位通气联合反特伦德伦伯格卧位2 h,然后单独采用俯卧位通气2 h。比较三组患者的肺通气功能及呼吸系统的静态顺应性(static compliance of the total respiratory system,Ctot)、胸廓静态顺应性(static compliance of the chest wall,Ccw)情况。【结果】三组患者干预前氧合指数(PaO_(2)/FiO_(2))、动脉二氧化碳分压(PaCO_(2))比较,差异均无统计学意义(P>0.05);干预2 h后,C组PaO_(2)/FiO_(2)高于A组(P<0.05),PaCO_(2)低于A组、B组(P<0.05);干预4 h后,三组PaO_(2)/FiO_(2)比较,差异均有统计学意义(P<0.05),但三组PaCO_(2)比较,差异无统计学意义(P>0.05)。三组患者干预前Ctot、Ccw比较,差异均无统计学意义(P>0.05);干预2 h后,C组Ccw高于A组、B组(P<0.05);干预4 h后,C组Ccw显著高于A组(P<0.05)。【结论】俯卧位通气前采用俯卧位联合反特伦德伦伯格卧位可缩短ARDS患者PaO_(2)/FiO_(2)恢复时间,增加Ccw,在护理过程中应综合考虑患者气道及呼吸管理需求,选择合适体位。
基金Supported by grants from the Science and Technology Development Project of Yantai(No.2008162)Natural Sciences Foundation of Shandong(No.ZR2013HL004)Science and Technology Important Project of Binzhou Medical College(No.BY20bkjzd04)
文摘Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.
