目的研究疼痛对大鼠胃、结肠钙素基因相关肽(calcitonin gene related peptide,CGRP)和P物质(substance P,SP)的影响。方法动物随机分成空白对照组和疼痛模型组,左足跖皮下注射5%甲醛50μl建立大鼠疼痛模型,采用放射免疫分析法测定血浆...目的研究疼痛对大鼠胃、结肠钙素基因相关肽(calcitonin gene related peptide,CGRP)和P物质(substance P,SP)的影响。方法动物随机分成空白对照组和疼痛模型组,左足跖皮下注射5%甲醛50μl建立大鼠疼痛模型,采用放射免疫分析法测定血浆、胃和结肠中CGRP和SP含量。结果疼痛模型组大鼠血浆中及胃、结肠CGRP和SP含量与空白对照组差别有统计学意义(p<0.01)结论大鼠左足跖皮下注射甲醛可致痛症状产生,并引起胃、结肠CGRP含量升高及SP含量下降。进而抑制大鼠胃、结肠蠕动,产生消化不良症状。展开更多
AIM： To evaluate the therapeutic effects of a probiotic supplement （Clostridium butyricum, CGMCC0313 ） in a chemically-induced rat model of experimental colitis. METHODS： An experimental ulcerative colitis model w...AIM： To evaluate the therapeutic effects of a probiotic supplement （Clostridium butyricum, CGMCC0313 ） in a chemically-induced rat model of experimental colitis. METHODS： An experimental ulcerative colitis model was established by rectal injection of oxazolone into the colon of 40 Wistar rats randomly divided into four groups. The positive control group was sacrificed 3 d after colitis onset. The remaining groups were fed daily with either 2 mL of C. butyricum （2.3 × 10^11 CFU/L）, 2 mL of mesalamine （100 g/L）, or 1 mL of sodium butyrate （50 mmol/L） for 21 d. The animals＇ body weight, behavior, and bowel movements were recorded weekly. After sacrifice, visual and microscopic observations of pathological changes of colon tissue were made, body weight and wet colon mass index were measured and recorded, and serum levels of interleukin-23 （IL-23） and TNF-α were measured using ELISA. Expression of calcitonin gene-related peptide in colon tissue was measured by RT-PCR. Finally, changes in rat intestinal microflora status were measured in all groups.lowered the serum levels of both IL-23 and tumor necrosis factor-α （TNF-α） with similar or even better efficiency than that of mesalamine or sodium butyrate. The rat intestinal flora appeared to recover more quickly in the group treated with C. butyricum than in the mesalamine and sodium butyrate groups. Finally, we found that the expression level of calcitonin gene related peptide was elevated in colon tissue in the sodium butyrate treated group but not in the C. butyricum or mesalamine treated groups, indicating a sensitization of colon following sodium butyrate treatment. CONCLUSION： In our experimental colitis model, treatment with C. butyricum CGMCC0313, a probiotic supplement, is at least as efficient as treatment with mesalamine.展开更多
文摘目的研究疼痛对大鼠胃、结肠钙素基因相关肽(calcitonin gene related peptide,CGRP)和P物质(substance P,SP)的影响。方法动物随机分成空白对照组和疼痛模型组,左足跖皮下注射5%甲醛50μl建立大鼠疼痛模型,采用放射免疫分析法测定血浆、胃和结肠中CGRP和SP含量。结果疼痛模型组大鼠血浆中及胃、结肠CGRP和SP含量与空白对照组差别有统计学意义(p<0.01)结论大鼠左足跖皮下注射甲醛可致痛症状产生,并引起胃、结肠CGRP含量升高及SP含量下降。进而抑制大鼠胃、结肠蠕动,产生消化不良症状。
基金The Model Subject of High-Tech Industrializing Conversion of Series Microecological New Drugs,National Development and Reform Commission High-Tech,[2004]2078
文摘AIM： To evaluate the therapeutic effects of a probiotic supplement （Clostridium butyricum, CGMCC0313 ） in a chemically-induced rat model of experimental colitis. METHODS： An experimental ulcerative colitis model was established by rectal injection of oxazolone into the colon of 40 Wistar rats randomly divided into four groups. The positive control group was sacrificed 3 d after colitis onset. The remaining groups were fed daily with either 2 mL of C. butyricum （2.3 × 10^11 CFU/L）, 2 mL of mesalamine （100 g/L）, or 1 mL of sodium butyrate （50 mmol/L） for 21 d. The animals＇ body weight, behavior, and bowel movements were recorded weekly. After sacrifice, visual and microscopic observations of pathological changes of colon tissue were made, body weight and wet colon mass index were measured and recorded, and serum levels of interleukin-23 （IL-23） and TNF-α were measured using ELISA. Expression of calcitonin gene-related peptide in colon tissue was measured by RT-PCR. Finally, changes in rat intestinal microflora status were measured in all groups.lowered the serum levels of both IL-23 and tumor necrosis factor-α （TNF-α） with similar or even better efficiency than that of mesalamine or sodium butyrate. The rat intestinal flora appeared to recover more quickly in the group treated with C. butyricum than in the mesalamine and sodium butyrate groups. Finally, we found that the expression level of calcitonin gene related peptide was elevated in colon tissue in the sodium butyrate treated group but not in the C. butyricum or mesalamine treated groups, indicating a sensitization of colon following sodium butyrate treatment. CONCLUSION： In our experimental colitis model, treatment with C. butyricum CGMCC0313, a probiotic supplement, is at least as efficient as treatment with mesalamine.
