AIM To study the inhibition of oxygen consumption by annonaceous acetogenins (ACG) and their structure activity relationship (SAR). METHODS The inhibition of oxygen consumption in chicken liver cell respiration by dif...AIM To study the inhibition of oxygen consumption by annonaceous acetogenins (ACG) and their structure activity relationship (SAR). METHODS The inhibition of oxygen consumption in chicken liver cell respiration by different structural ACG was studied by using oxygen electrode technique. RESULTS Six ACG showed potent inhibitory effects like rotenone which was a classical inhibitor of mitochondrial complex I, and was more potent than complex IV inhibitor KCN. The IC 50 values of six ACG for inhibiting oxygen consumption suggested that bis tetrahydrofuran (THF) ACG was 7～11 times more active than non THF ACG, and A 1 type ACG was more potent than A 2 type ACG. CONCLUSION The terminal γ lactone was crucial for the inhibition of oxygen consumption. The distance between THF and γ lactone, the hydroxyl groups in the alkyl chain, were the important factors of SAR, but the 4 OH group possibly played some negative role in the exhibit of potent activity.展开更多
The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investi...The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.展开更多
文摘AIM To study the inhibition of oxygen consumption by annonaceous acetogenins (ACG) and their structure activity relationship (SAR). METHODS The inhibition of oxygen consumption in chicken liver cell respiration by different structural ACG was studied by using oxygen electrode technique. RESULTS Six ACG showed potent inhibitory effects like rotenone which was a classical inhibitor of mitochondrial complex I, and was more potent than complex IV inhibitor KCN. The IC 50 values of six ACG for inhibiting oxygen consumption suggested that bis tetrahydrofuran (THF) ACG was 7～11 times more active than non THF ACG, and A 1 type ACG was more potent than A 2 type ACG. CONCLUSION The terminal γ lactone was crucial for the inhibition of oxygen consumption. The distance between THF and γ lactone, the hydroxyl groups in the alkyl chain, were the important factors of SAR, but the 4 OH group possibly played some negative role in the exhibit of potent activity.
基金Supported by the National Natural Science Foundation of China(30371121)the Natural Science Foundation of Chongqing the Educa-tion Committee Foundation of Chongqing~~
文摘The objective of the study was to explore the effect of acute low temperature stress on VO2 and Vf of Silurus meridionalis and Pelteobag vachelli after 10 minutes cold water bath with different temperature.The investigation was operated under the temperature of 24 ℃.It was found that the VO2 and Vf of Silurus meridionalis after 6 and 0 ℃ stress showed a decrease-increase-decrease trend while other groups showed a rapid increase then slowly recovery trend.The VO2 and Vf of Pelteobag vachelli after 0 ℃stress showed a decrease-increase-decrease trend while other groups showed an increase then slowly recovery process.It was suggested that Pelteobag vachelli was more adaptive to acute cold stress,but it cost more energy adapting to cold stress compared to Silurus meridionalis.
