为了探究β-罗勒烯对烟田烟蚜种群的控制效果及最佳施用浓度,在室内条件下分析了不同浓度β-罗勒烯对烟蚜种群数量、种群增长率、干扰作用控制指数以及趋避效果的影响。通过田间药效试验分析了β-罗勒烯及70%吡虫啉水分散粒剂对烟蚜种...为了探究β-罗勒烯对烟田烟蚜种群的控制效果及最佳施用浓度,在室内条件下分析了不同浓度β-罗勒烯对烟蚜种群数量、种群增长率、干扰作用控制指数以及趋避效果的影响。通过田间药效试验分析了β-罗勒烯及70%吡虫啉水分散粒剂对烟蚜种群数量的影响。室内试验结果表明,10 mM β-罗勒烯对烟蚜种群控制效果最佳,相较于对照,施用β-罗勒烯后12 d每株烤烟上烟蚜密度减少81.7头/株。田间药效试验结果表明,β-罗勒烯能有效抑制田间烟蚜种群数量,综合效果优于化学农药。进一步的RT-qPCR分析结果表明,β-罗勒烯处理能显著提高烤烟植株中水杨酸途径指示基因PR1和茉莉酸/乙烯途径指示基因PDF1.2的表达量,这些说明β-罗勒烯能通过同时激活多条防御途径以促进烤烟植株对烟蚜的防御能力。研究结果表明10 mM β-罗勒烯可用于对烟田烟蚜的控制,本研究为烟田绿色防控烟蚜提供了新的途径。展开更多
β-罗勒烯是一种能诱导植物产生防御反应的植物通讯信号分子。本文是为了探究β-罗勒烯对水稻二化螟的防治效果及其对产量影响的研究。在大田条件下,同时分析了β-罗勒烯与常规化学农药对水稻二化螟的防治效果以及对水稻产量的影响。大...β-罗勒烯是一种能诱导植物产生防御反应的植物通讯信号分子。本文是为了探究β-罗勒烯对水稻二化螟的防治效果及其对产量影响的研究。在大田条件下,同时分析了β-罗勒烯与常规化学农药对水稻二化螟的防治效果以及对水稻产量的影响。大田试验结果显示:经β-罗勒烯处理后,水稻的生长发育和农艺性状均未受到影响,并且β-罗勒烯对水稻二化螟的防治效果优于化学农药,早期枯鞘株率与后期白穗株率均低于空白对照,对枯鞘与白穗的防效可达56.81%、41.33%,相比于化学农药早期枯鞘株率降低了2.15%;后期白穗率相对于化学农药降低了0.7%。β-罗勒烯与对照相比水稻产量提升了16.83%,与化学农药无显著差异。以上结果均表明,β-罗勒烯在防治水稻二化螟方面具有显著功效。本研究为绿色防治水稻二化螟提供了新的途径。β-Ocimene is a plant communication signal molecule capable of inducing defense responses in plants. This paper aims to study the control effect of β-basil on rice dicarborer and its effect on yield. Under field conditions, the control effects of β-Ocimene and conventional chemical pesticides on the Chilo suppressalis in rice and their influences on rice yield were analyzed simultaneously. The field experiment results indicated that the growth and agronomic traits of rice were not affected after treatment with β-Ocimene. Moreover, the control effect of β-Ocimene against the Chilo suppressalis in rice was superior to that of chemical pesticides. The rates of early dead sheath plants and late white panicle plants were both lower than those of the blank control. The control efficacy against dead sheath and white panicle could reach 56.81% and 41.33%, respectively. Compared with chemical pesticides, the rate of early dead sheath plants was reduced by 2.15%;the rate of late white panicle plants was 0.7% lower than that of chemical pesticides. The rice yield increased by 16.83% with β-Ocimene compared to the control, and there was no significant difference from chemical pesticides. The above results all suggest that β-Ocimene has significant efficacy in controlling the Chilo suppressalis in rice. This study provides a new approach for the green control of the Chilo suppressalis in rice.展开更多
β-罗勒烯作为一种在植物生理生态系统中起着关键通讯作用的信号分子,在防控烤烟烟草普通花叶病毒病(TMV)方面展现出了极为明显的效果。室内盆栽试验中,处理组发病率比对照组低49.6%,病情指数下降65.21%。大田试验中,β-罗勒烯效果优于...β-罗勒烯作为一种在植物生理生态系统中起着关键通讯作用的信号分子,在防控烤烟烟草普通花叶病毒病(TMV)方面展现出了极为明显的效果。室内盆栽试验中,处理组发病率比对照组低49.6%,病情指数下降65.21%。大田试验中,β-罗勒烯效果优于化学农药,控制效果达57.67%至64.71%。病情上升率统计显示,β-罗勒烯诱导的植株病情指数仅上升18.68%,远低于化学农药和对照组。这表明β-罗勒烯不仅在初始阶段有效防控TMV,还能持续抑制病情恶化。这一系列数据表明,β-罗勒烯为大田防控TMV提供了一种创新、高效的方法,减少化学农药依赖,推动绿色农业发展,为农业现代化提供支撑。β-Ocimene, as a signal molecule that plays a key communication role in the plant physiological ecosystem, has shown extremely obvious effects in the prevention and control of common Tobacco mosaic virus disease (TMV) in flue-cured tobacco. In the indoor pot experiment, the incidence rate of the treatment group was 49.6% lower than that of the control group, and the disease index decreased by 65.21%. In the field experiment, the effect of β-ocimene was better than that of chemical pesticides, and the control effect reached 57.67% to 64.71%. The statistics of the disease increase rate showed that the disease index of the plants induced by β-ocimene only increased by 18.68%, which was much lower than that of chemical pesticides and the control group. This indicates that β-ocimene not only effectively prevents and controls TMV in the initial stage, but also continuously inhibits the deterioration of the disease. This series of data shows that β-ocimene provides an innovative and efficient method for the field prevention and control of TMV, reduces the dependence on chemical pesticides, promotes the development of green agriculture, and provides support for agricultural modernization.展开更多
植物通讯信号分子β-罗勒烯在植物防御方面起着重要作用,受β-罗勒烯诱导快速高表达的SFIBO(snoRNAs Fast-Induced by Ocimene)基因的启动子特性有待研究。SFIBO基因起始密码子上游有877 bp,依据这段序列上已知的元件分布特征,将877 bp...植物通讯信号分子β-罗勒烯在植物防御方面起着重要作用,受β-罗勒烯诱导快速高表达的SFIBO(snoRNAs Fast-Induced by Ocimene)基因的启动子特性有待研究。SFIBO基因起始密码子上游有877 bp,依据这段序列上已知的元件分布特征,将877 bp分成三段:-877~-1 bp、-637~-1 bp和-277~-1 bp。将这3个片段分别扩增后与GUS报告基因构建融合表达框,并利用浸花法将这3个植物双元表达载体转化拟南芥Col-0。对GUS组织化学染色进行分析,结果显示SFIBO基因的有效启动子为起始密码子上游637 bp DNA片段,β-罗勒烯诱导反应的顺式作用元件在-637~-277 bp之间的这360 bp DNA序列上。以上结果为下一步确定β-罗勒烯响应元件的具体序列和研究SFIBO基因的功能提供了科学依据。展开更多
文摘为了探究β-罗勒烯对烟田烟蚜种群的控制效果及最佳施用浓度,在室内条件下分析了不同浓度β-罗勒烯对烟蚜种群数量、种群增长率、干扰作用控制指数以及趋避效果的影响。通过田间药效试验分析了β-罗勒烯及70%吡虫啉水分散粒剂对烟蚜种群数量的影响。室内试验结果表明,10 mM β-罗勒烯对烟蚜种群控制效果最佳,相较于对照,施用β-罗勒烯后12 d每株烤烟上烟蚜密度减少81.7头/株。田间药效试验结果表明,β-罗勒烯能有效抑制田间烟蚜种群数量,综合效果优于化学农药。进一步的RT-qPCR分析结果表明,β-罗勒烯处理能显著提高烤烟植株中水杨酸途径指示基因PR1和茉莉酸/乙烯途径指示基因PDF1.2的表达量,这些说明β-罗勒烯能通过同时激活多条防御途径以促进烤烟植株对烟蚜的防御能力。研究结果表明10 mM β-罗勒烯可用于对烟田烟蚜的控制,本研究为烟田绿色防控烟蚜提供了新的途径。
文摘β-罗勒烯是一种能诱导植物产生防御反应的植物通讯信号分子。本文是为了探究β-罗勒烯对水稻二化螟的防治效果及其对产量影响的研究。在大田条件下,同时分析了β-罗勒烯与常规化学农药对水稻二化螟的防治效果以及对水稻产量的影响。大田试验结果显示:经β-罗勒烯处理后,水稻的生长发育和农艺性状均未受到影响,并且β-罗勒烯对水稻二化螟的防治效果优于化学农药,早期枯鞘株率与后期白穗株率均低于空白对照,对枯鞘与白穗的防效可达56.81%、41.33%,相比于化学农药早期枯鞘株率降低了2.15%;后期白穗率相对于化学农药降低了0.7%。β-罗勒烯与对照相比水稻产量提升了16.83%,与化学农药无显著差异。以上结果均表明,β-罗勒烯在防治水稻二化螟方面具有显著功效。本研究为绿色防治水稻二化螟提供了新的途径。β-Ocimene is a plant communication signal molecule capable of inducing defense responses in plants. This paper aims to study the control effect of β-basil on rice dicarborer and its effect on yield. Under field conditions, the control effects of β-Ocimene and conventional chemical pesticides on the Chilo suppressalis in rice and their influences on rice yield were analyzed simultaneously. The field experiment results indicated that the growth and agronomic traits of rice were not affected after treatment with β-Ocimene. Moreover, the control effect of β-Ocimene against the Chilo suppressalis in rice was superior to that of chemical pesticides. The rates of early dead sheath plants and late white panicle plants were both lower than those of the blank control. The control efficacy against dead sheath and white panicle could reach 56.81% and 41.33%, respectively. Compared with chemical pesticides, the rate of early dead sheath plants was reduced by 2.15%;the rate of late white panicle plants was 0.7% lower than that of chemical pesticides. The rice yield increased by 16.83% with β-Ocimene compared to the control, and there was no significant difference from chemical pesticides. The above results all suggest that β-Ocimene has significant efficacy in controlling the Chilo suppressalis in rice. This study provides a new approach for the green control of the Chilo suppressalis in rice.
文摘β-罗勒烯作为一种在植物生理生态系统中起着关键通讯作用的信号分子,在防控烤烟烟草普通花叶病毒病(TMV)方面展现出了极为明显的效果。室内盆栽试验中,处理组发病率比对照组低49.6%,病情指数下降65.21%。大田试验中,β-罗勒烯效果优于化学农药,控制效果达57.67%至64.71%。病情上升率统计显示,β-罗勒烯诱导的植株病情指数仅上升18.68%,远低于化学农药和对照组。这表明β-罗勒烯不仅在初始阶段有效防控TMV,还能持续抑制病情恶化。这一系列数据表明,β-罗勒烯为大田防控TMV提供了一种创新、高效的方法,减少化学农药依赖,推动绿色农业发展,为农业现代化提供支撑。β-Ocimene, as a signal molecule that plays a key communication role in the plant physiological ecosystem, has shown extremely obvious effects in the prevention and control of common Tobacco mosaic virus disease (TMV) in flue-cured tobacco. In the indoor pot experiment, the incidence rate of the treatment group was 49.6% lower than that of the control group, and the disease index decreased by 65.21%. In the field experiment, the effect of β-ocimene was better than that of chemical pesticides, and the control effect reached 57.67% to 64.71%. The statistics of the disease increase rate showed that the disease index of the plants induced by β-ocimene only increased by 18.68%, which was much lower than that of chemical pesticides and the control group. This indicates that β-ocimene not only effectively prevents and controls TMV in the initial stage, but also continuously inhibits the deterioration of the disease. This series of data shows that β-ocimene provides an innovative and efficient method for the field prevention and control of TMV, reduces the dependence on chemical pesticides, promotes the development of green agriculture, and provides support for agricultural modernization.
文摘植物通讯信号分子β-罗勒烯在植物防御方面起着重要作用,受β-罗勒烯诱导快速高表达的SFIBO(snoRNAs Fast-Induced by Ocimene)基因的启动子特性有待研究。SFIBO基因起始密码子上游有877 bp,依据这段序列上已知的元件分布特征,将877 bp分成三段:-877~-1 bp、-637~-1 bp和-277~-1 bp。将这3个片段分别扩增后与GUS报告基因构建融合表达框,并利用浸花法将这3个植物双元表达载体转化拟南芥Col-0。对GUS组织化学染色进行分析,结果显示SFIBO基因的有效启动子为起始密码子上游637 bp DNA片段,β-罗勒烯诱导反应的顺式作用元件在-637~-277 bp之间的这360 bp DNA序列上。以上结果为下一步确定β-罗勒烯响应元件的具体序列和研究SFIBO基因的功能提供了科学依据。
