[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to A...[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to Arabidopsis grain development and their homologous rape EST sequences. After electrophoresis, 18 pairs of ACGM primers were selected for the clustering analysis of 16 larger grained samples and four fine grained samples of rapeseed. [Result] PCR result showed that 2-6 specific bands were respectively amplified by each pair of primes, and all the bands were polymorphic and repeatable, suggesting that the optimized ACGM markers were useful for clustering analysis of B. napus species. Clustering analysis revealed that the 20 rapeseed samples were divided into three clusters A, B, and C at similarity coefficient 0.6. Then, the clusters A and B were further divided into five sub clusters A1, A2, A3, B1 and B2 at similarity coefficient 0.67. [Conclusion] This study will provide theoretical and practical values for rape breeding.展开更多
The ongoing need for better fuel economy and lower exhaust pollution of vehicles has increased the employment of electric power steering(EPS)in automotives.Optimal design of EPS for a product family reduces the develo...The ongoing need for better fuel economy and lower exhaust pollution of vehicles has increased the employment of electric power steering(EPS)in automotives.Optimal design of EPS for a product family reduces the development and fabrication costs significantly.In this paper,the TOPSIS method along with the NSGA-Ⅱis employed to find an optimum family of EPS for an automotive platform.A multi-objective optimization problem is defined considering road feel,steering portability,RMS of Ackerman error,and product family penalty function(PFPF)as the conflicting objective functions.The results for the single objective optimization problems and the ones for the multi-objective optimization problem,as well as two suggested trade-off design points are presented,compared and discussed.For the two suggested points,performance at one objective function is deteriorated by about 1%,while the commonality is increased by 20%–40%,which shows the effectiveness of the proposed method in first finding the non-dominated design points and then selecting the trade-off among the obtained points.The results indicate that the obtained trade-off points have superior performance within the product family with maximum number of common parts.展开更多
基金Supported by the National Natural Science Foundation of China(30860147)Open Funds of National Key Laboratory of Crop Genetic Improvement(ZK200902)Natural Science Foundation of Yunnan Province(2011FB117)~~
文摘[Objective] This study aimed to develop ACGM markers for the clustering analysis of large grained Brassica napus materials. [Method] A total of 44 pairs of ACGM primers were designed according to 18 genes related to Arabidopsis grain development and their homologous rape EST sequences. After electrophoresis, 18 pairs of ACGM primers were selected for the clustering analysis of 16 larger grained samples and four fine grained samples of rapeseed. [Result] PCR result showed that 2-6 specific bands were respectively amplified by each pair of primes, and all the bands were polymorphic and repeatable, suggesting that the optimized ACGM markers were useful for clustering analysis of B. napus species. Clustering analysis revealed that the 20 rapeseed samples were divided into three clusters A, B, and C at similarity coefficient 0.6. Then, the clusters A and B were further divided into five sub clusters A1, A2, A3, B1 and B2 at similarity coefficient 0.67. [Conclusion] This study will provide theoretical and practical values for rape breeding.
文摘The ongoing need for better fuel economy and lower exhaust pollution of vehicles has increased the employment of electric power steering(EPS)in automotives.Optimal design of EPS for a product family reduces the development and fabrication costs significantly.In this paper,the TOPSIS method along with the NSGA-Ⅱis employed to find an optimum family of EPS for an automotive platform.A multi-objective optimization problem is defined considering road feel,steering portability,RMS of Ackerman error,and product family penalty function(PFPF)as the conflicting objective functions.The results for the single objective optimization problems and the ones for the multi-objective optimization problem,as well as two suggested trade-off design points are presented,compared and discussed.For the two suggested points,performance at one objective function is deteriorated by about 1%,while the commonality is increased by 20%–40%,which shows the effectiveness of the proposed method in first finding the non-dominated design points and then selecting the trade-off among the obtained points.The results indicate that the obtained trade-off points have superior performance within the product family with maximum number of common parts.
