在社区治理领域,社区服务是核心议题。深入探究社区服务如何满足居民的“尊重需求”意义重大。本研究先综述社区服务及其需求,明确“尊重需求”在其中的重要性,该需求的满足有助于提升居民获得感、幸福感与安全感。然后引入秦川社区服...在社区治理领域,社区服务是核心议题。深入探究社区服务如何满足居民的“尊重需求”意义重大。本研究先综述社区服务及其需求,明确“尊重需求”在其中的重要性,该需求的满足有助于提升居民获得感、幸福感与安全感。然后引入秦川社区服务实践验证,探讨需求层次理论和人本主义需要理论在社区高质量服务中的内涵。研究表明高质量社区服务涵盖多方面内容,应基于居民需求构建分层分类、差异化、专业化且具针对性的服务体系,将需求理论应用于服务实践。In the realm of community governance, community service stands as a pivotal topic. Delving into how community services fulfill residents’ “need for respect” holds immense importance. This study initially examines community services and their corresponding needs, underscoring the significance of “need for respect” within this context. Fulfilling this need significantly boosts residents’ sense of gain, happiness, and security. Subsequently, the Qinchuan community service practice is introduced to validate and explore the implications of the hierarchy of needs theory and humanistic needs theory in delivering high-quality community services. Research indicates that high-quality community services encompass a wide range of aspects. Therefore, it is imperative to construct a hierarchical, differentiated, specialized, and targeted service system tailored to residents’ needs, thereby applying demand theory to service practice.展开更多
为探究秦川牛宰后成熟期间基础免疫球蛋白(basigin,BSG)对丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)信号通路及细胞凋亡的影响,利用4D-非标记定量组学技术分析BSG及其差异蛋白的变化情况。向宰后秦川牛肉的背最长肌...为探究秦川牛宰后成熟期间基础免疫球蛋白(basigin,BSG)对丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)信号通路及细胞凋亡的影响,利用4D-非标记定量组学技术分析BSG及其差异蛋白的变化情况。向宰后秦川牛肉的背最长肌中注射BSG抑制剂衣霉素,通过蛋白质免疫印迹法测定秦川牛肉在4℃贮藏过程中MAPK通路关键蛋白质表达水平,并测定caspase-3的活力变化。研究表明:在秦川牛宰后贮藏期内,BSG的表达量总体呈先上升后下降趋势;利用京都基因与基因组百科全书通路分析发现BSG及其差异蛋白质显著注释于氧化磷酸化通路、钙信号通路、MAPK信号通路,说明BSG通过MAPK途径发挥作用。另外,衣霉素组MAPK通路关键蛋白质的相对表达量均显著下调,说明BSG抑制剂使得MAPK信号通路失活。这为研究BSG对MAPK信号通路影响奠定了很好的基础。在抑制BSG的表达后,衣霉素组caspase-3的活力明显上升,说明细胞凋亡是细胞损伤机制的重要环节,衣霉素作用于BSG的N端结构使蛋白质发生去糖基化作用。通过抑制细胞内蛋白质的折叠使其生物学活性受到抑制,从而诱导细胞凋亡。展开更多
文摘在社区治理领域,社区服务是核心议题。深入探究社区服务如何满足居民的“尊重需求”意义重大。本研究先综述社区服务及其需求,明确“尊重需求”在其中的重要性,该需求的满足有助于提升居民获得感、幸福感与安全感。然后引入秦川社区服务实践验证,探讨需求层次理论和人本主义需要理论在社区高质量服务中的内涵。研究表明高质量社区服务涵盖多方面内容,应基于居民需求构建分层分类、差异化、专业化且具针对性的服务体系,将需求理论应用于服务实践。In the realm of community governance, community service stands as a pivotal topic. Delving into how community services fulfill residents’ “need for respect” holds immense importance. This study initially examines community services and their corresponding needs, underscoring the significance of “need for respect” within this context. Fulfilling this need significantly boosts residents’ sense of gain, happiness, and security. Subsequently, the Qinchuan community service practice is introduced to validate and explore the implications of the hierarchy of needs theory and humanistic needs theory in delivering high-quality community services. Research indicates that high-quality community services encompass a wide range of aspects. Therefore, it is imperative to construct a hierarchical, differentiated, specialized, and targeted service system tailored to residents’ needs, thereby applying demand theory to service practice.
文摘为探究秦川牛宰后成熟期间基础免疫球蛋白(basigin,BSG)对丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)信号通路及细胞凋亡的影响,利用4D-非标记定量组学技术分析BSG及其差异蛋白的变化情况。向宰后秦川牛肉的背最长肌中注射BSG抑制剂衣霉素,通过蛋白质免疫印迹法测定秦川牛肉在4℃贮藏过程中MAPK通路关键蛋白质表达水平,并测定caspase-3的活力变化。研究表明:在秦川牛宰后贮藏期内,BSG的表达量总体呈先上升后下降趋势;利用京都基因与基因组百科全书通路分析发现BSG及其差异蛋白质显著注释于氧化磷酸化通路、钙信号通路、MAPK信号通路,说明BSG通过MAPK途径发挥作用。另外,衣霉素组MAPK通路关键蛋白质的相对表达量均显著下调,说明BSG抑制剂使得MAPK信号通路失活。这为研究BSG对MAPK信号通路影响奠定了很好的基础。在抑制BSG的表达后,衣霉素组caspase-3的活力明显上升,说明细胞凋亡是细胞损伤机制的重要环节,衣霉素作用于BSG的N端结构使蛋白质发生去糖基化作用。通过抑制细胞内蛋白质的折叠使其生物学活性受到抑制,从而诱导细胞凋亡。
