A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end ...A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end of the RNA Transcript） technique. The titer of the original cDNA library was about 1.5 × 10^6 cfu·mL^-1 and the average insertion size was about 1 kb with a high recombination rate （97%）. The 5011 high-quality expressed sequence tags （ESTs） were obtained from 5393 randomly picked cDNA clones. Clustering and assembly of ESTs resulted in 2984 unique sequences, consisting of 618 contigs and 2366 singlets. EST sequence annotation revealed that 2333 and 1901 unigenes were functionally anno- tated in the NCBI non-redundant database and Swiss-Prot protein database, respectively. Functional analysis demonstrated that 1495 （50.1%） unigenes were associated with 4082 Gene Ontology （GO） terms. A total of 847 unigenes were grouped into 22 Cluster of Orthologous Groups （COG） functional categories. Based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase （RLK） genes were screened. Furthermore, a total of 94 simple sequence repeats （SSRs） were discovered, of which 20 loci were successfully amplified in C debaoensis. This study is the first EST analysis for the coralloid roots of C debaoensis and provides a valuable genomic resource for novel gene discovery, gene expression and comparative genomics, conservation and management studies as well as applications in C debaoensis and related cycad species.展开更多
Complexity of the systems in everyday life of modern man continuously increases, as the monitoring and the management are concentrated on and depended on the reactions of one operator or a group of operators. Sometime...Complexity of the systems in everyday life of modern man continuously increases, as the monitoring and the management are concentrated on and depended on the reactions of one operator or a group of operators. Sometimes because of human errors in extreme situations, it increases the potential risk for life of large groups of people and of the operators. This requires continuous improvement of the systems for psycho-physiological assessment by developing the new efficient methods involving known and new indicators of psycho-physiological state of the individual. The complex BeOn-1 is a new computer-based experimental and applied system for examination of situational vigilance and behavior of aviation specialists-pilots, navigators, operators of unmanned aerial vehicles in a complex operating environment with extreme impact factors. In the up-to-date systems for evaluating the operators, the test results are compared and analyzed together with a number of physiological parameters that are used as indicators of psycho-physiological status of the investigated subjects. In the ＂BeOn-1＂ they are indicators about the efficiency of individual stress coping strategy. BeOn-I allows us to study the individual skills of perception and the ability to act under extreme conditions of the operational environment and is a comfortable working methodology for daily needs in the selection, periodic monitoring of the operational staff and support to flight safety.展开更多
基金supported by the Grant(201522)from Shenzhen Urban Management
文摘A normalized full-length cDNA library was constructed from the coralloid roots of Cycas debaoensis by the DSN （duplex-specific nuclease） normalization method combined with the SMART （Switching Mechanism At 5＇ end of the RNA Transcript） technique. The titer of the original cDNA library was about 1.5 × 10^6 cfu·mL^-1 and the average insertion size was about 1 kb with a high recombination rate （97%）. The 5011 high-quality expressed sequence tags （ESTs） were obtained from 5393 randomly picked cDNA clones. Clustering and assembly of ESTs resulted in 2984 unique sequences, consisting of 618 contigs and 2366 singlets. EST sequence annotation revealed that 2333 and 1901 unigenes were functionally anno- tated in the NCBI non-redundant database and Swiss-Prot protein database, respectively. Functional analysis demonstrated that 1495 （50.1%） unigenes were associated with 4082 Gene Ontology （GO） terms. A total of 847 unigenes were grouped into 22 Cluster of Orthologous Groups （COG） functional categories. Based on the EST dataset, 22 ESTs that encoded putative receptor-like protein kinase （RLK） genes were screened. Furthermore, a total of 94 simple sequence repeats （SSRs） were discovered, of which 20 loci were successfully amplified in C debaoensis. This study is the first EST analysis for the coralloid roots of C debaoensis and provides a valuable genomic resource for novel gene discovery, gene expression and comparative genomics, conservation and management studies as well as applications in C debaoensis and related cycad species.
文摘Complexity of the systems in everyday life of modern man continuously increases, as the monitoring and the management are concentrated on and depended on the reactions of one operator or a group of operators. Sometimes because of human errors in extreme situations, it increases the potential risk for life of large groups of people and of the operators. This requires continuous improvement of the systems for psycho-physiological assessment by developing the new efficient methods involving known and new indicators of psycho-physiological state of the individual. The complex BeOn-1 is a new computer-based experimental and applied system for examination of situational vigilance and behavior of aviation specialists-pilots, navigators, operators of unmanned aerial vehicles in a complex operating environment with extreme impact factors. In the up-to-date systems for evaluating the operators, the test results are compared and analyzed together with a number of physiological parameters that are used as indicators of psycho-physiological status of the investigated subjects. In the ＂BeOn-1＂ they are indicators about the efficiency of individual stress coping strategy. BeOn-I allows us to study the individual skills of perception and the ability to act under extreme conditions of the operational environment and is a comfortable working methodology for daily needs in the selection, periodic monitoring of the operational staff and support to flight safety.
