Fluorescence anisotropy was employed for the investigation of the association between AlS 4Pc and BSA. The average binding number of AlS 4Pc to BSA was calculated and the constants of association reaction occurring in...Fluorescence anisotropy was employed for the investigation of the association between AlS 4Pc and BSA. The average binding number of AlS 4Pc to BSA was calculated and the constants of association reaction occurring in the medium of pH 7 0 were shown. The results revealed that the interaction between AlS 4Pc and BSA was strong, which implied that AlS 4Pc may be stored and transferred by serum albumin. The merits of the method used were also pointed out.展开更多
文摘采用控制酯化率和非外加相转移催化剂在敞开体系中进行反应的方法,合成了月桂醇聚氧乙烯醚(9)辛基磺基琥珀酸混合双酯钠。最佳工艺条件为:n〔月桂醇聚氧乙烯醚(9)〕∶n(顺酐)=1.00∶1.10,于140℃单酯化反应2.0 h,得到产率为99.8%的单酯化产物。n(2-乙基己醇)∶n(顺酐)=4.0∶1.0,于加热介质温度240℃条件下双酯化反应5.5 h,得到产率为94.4%的双酯化产物。n(亚硫酸氢钠)∶n(顺酐)=1.10∶1.00,加热介质温度210℃条件下,磺化反应6 h得终产物。测得产物的表面张力为32.5 mN/m、临界胶束浓度为1.1×10-4mol/L、耐硬水能力为24 m in、乳化力为3.3 m in、渗透力为3 s、分散力为90.5%、钙皂分散力为13%、去油污力为99.1%。与磺基琥珀酸二辛酯钠盐(快T)和月桂醇聚氧乙烯醚(9)磺基琥珀酸单酯二钠盐(LESS)进行性能对比,结果表明:耐硬水能力、分散力、钙皂分散性能和去油污性能较快T均得到了改善,渗透性较LESS得到了提高。
文摘Fluorescence anisotropy was employed for the investigation of the association between AlS 4Pc and BSA. The average binding number of AlS 4Pc to BSA was calculated and the constants of association reaction occurring in the medium of pH 7 0 were shown. The results revealed that the interaction between AlS 4Pc and BSA was strong, which implied that AlS 4Pc may be stored and transferred by serum albumin. The merits of the method used were also pointed out.
