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苹果与梨远缘杂交种甘金和甘红果实品质特性评价及分子水平鉴定 被引量:2
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作者 孙文泰 董铁 +1 位作者 王萍 马明 《果树学报》 CAS CSCD 北大核心 2023年第12期2505-2512,共8页
【目的】对苹果与梨远缘杂交的优良品种甘金和优系甘红进行果实品质特性评价,并用特异性分子标记鉴定杂交种的真实性,为后期真杂种在抗逆育种研究领域应用提供理论支撑。【方法】对甘金和甘红树体的生长势和果实经济性状进行评价,将苹... 【目的】对苹果与梨远缘杂交的优良品种甘金和优系甘红进行果实品质特性评价,并用特异性分子标记鉴定杂交种的真实性,为后期真杂种在抗逆育种研究领域应用提供理论支撑。【方法】对甘金和甘红树体的生长势和果实经济性状进行评价,将苹果金冠和西洋梨巴梨的基因组进行比对,分别筛选苹果和梨中特有的序列,通过设计属间特异性引物对甘金、甘红和亲本的DNA进行扩增。【结果】甘金和甘红树势生长健壮、抗逆性强和果实品质优。特异性引物M1、M2和M3在母本苹果品种中扩增出条带;P1、P2、P3这3对引物只能在父本梨品种中扩增出条带;2对通用引物U1和U2在苹果和梨杂交种中均能扩增出条带。此外,苹果M1、M2、M3和梨P1、P2、P3对杂交后代甘红、甘金进行扩增时,均出现条带,说明杂交后代既有苹果的基因,又有梨的基因。【结论】采用基因特异性分子标记开发的梨和苹果属间的特异性引物,鉴定远缘杂种的真实性,为苹果和梨以及其他果树的属间远缘杂交种的鉴定提供有价值的参考。 展开更多
关键词 苹果 甘金 甘红 远缘杂交 基因特异性分子标记
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苹果×梨属间杂交新品种‘甘金’的选育 被引量:10
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作者 张修仁 张居里 张世明 《果树科学》 CSCD 北大核心 1991年第2期65-70,共6页
甘金系元帅苹果(Malus pumila cv.Delicious)为母本,苹果梨(pyrus prifoliacv. Pingguoli)作父本杂交选育而成。通过花器官和果实心室变异性状、过氧化物酶同工酶和花药孢粉扫描电镜观察检查,证明系元帅苹果与苹果梨的属间杂种。甘金树... 甘金系元帅苹果(Malus pumila cv.Delicious)为母本,苹果梨(pyrus prifoliacv. Pingguoli)作父本杂交选育而成。通过花器官和果实心室变异性状、过氧化物酶同工酶和花药孢粉扫描电镜观察检查,证明系元帅苹果与苹果梨的属间杂种。甘金树势强健、腋花芽结果、丰产优质,花芽越冬性强,抗寒,适应性强。果实平均重200g,果肉绿黄色,果心小,肉质脆嫩味香甜,品质极上,可溶性固形物15%。在窑洞通风窖中可贮藏到次年3月,不绵、不沙、不皱皮。以垂丝海棠(Malus halliana)作砧木,耐旱性强,是河西走廊广大地区适宜栽培的苹果品种。 展开更多
关键词 苹果 属间杂交 品种 甘金 选育
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Species and Occurrence of Scarab in Sweet Potato Growing Areas of Xuzhou,Jiangsu
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作者 闫会 薛程 +2 位作者 张允刚 马代夫 李强 《Agricultural Science & Technology》 CAS 2015年第12期2742-2745,共4页
Frequency-vibrancy pest-killing lamp was used to calculate the numbers and types of scarabs during the growing period of sweet potato in Xuzhou area, thus to study the occurrence regularity of scarab and effect of met... Frequency-vibrancy pest-killing lamp was used to calculate the numbers and types of scarabs during the growing period of sweet potato in Xuzhou area, thus to study the occurrence regularity of scarab and effect of meteorological factor on its occurrence, which provided a reference and guidance for the control of grub in sweet potato field. The monitoring results indicated that scarabs in sweet potato planting area of Xuzhou mainly included Anomala corpulenta, Holotrichia parallela Motschulsky, Maladera verticalis and Holotrichia trichophora Fairm. Holotrichia tri- chophora Fairm has entered into its late stage at the early cultivation stage of sweet potato with a few occurrence quantities. The elongation stage of sweet potato vine is the full incidence period of imagoes of Maladera verticalis and Anomala cor- pulenta. Full incidence period of Holotrichia parallela Motschulsky is from late June to middle August, the outbreak cycle of which is long. The unearthed imagoes fol- low the regular change "many-few-many-few". Effects of meteorological factor on the activities of various scarabs are different. The activities of various scarabs are fre- quent in warm and windless sunny days. In rainy days, Holotrichia parallela Motschulsky, Holotrichia trichophora Fairm and Maladera verticalis are inactive. The numbers of Anomala corpulenta in cloudy days but not rainy days are higher than that in sunny days. 展开更多
关键词 Sweet potato SCARAB Occurrence regularity Meteorological factor
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理气开郁法板块方镇痛作用的动态分析 被引量:1
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作者 邢伟 李秀玲 +2 位作者 方步武 田在善 吴咸中 《中国中西医结合外科杂志》 CAS 2013年第5期537-541,共5页
目的:探讨理气开郁法中药板块方的镇痛作用特点。方法:将150只清洁级昆明种小鼠,按体重随机区组法分为15组,包括对照组,柴郁汤、芍甘汤、痛泻要方、金铃子散高中低剂量各组,吗啡和布洛芬组,采用45℃、50℃、55℃水浴引致小鼠疼痛模型,... 目的:探讨理气开郁法中药板块方的镇痛作用特点。方法:将150只清洁级昆明种小鼠,按体重随机区组法分为15组,包括对照组,柴郁汤、芍甘汤、痛泻要方、金铃子散高中低剂量各组,吗啡和布洛芬组,采用45℃、50℃、55℃水浴引致小鼠疼痛模型,动态测定痛阈潜伏期,用药前测定基础痛阈。采用重复测量设计的方差分析。结果:柴郁汤、芍甘汤和痛泻要方的镇痛作用以高剂量更明显;柴郁汤对45℃、50℃、55℃水浴的痛阈潜伏期均有延长作用,柴郁汤高、中剂量在多个时间点上显示出较好的镇痛作用。结论:柴郁汤的镇痛作用较强,作用特点类似于中枢镇痛药或兼有中枢性镇痛作用;芍药甘草汤、痛泻药方、金铃子散属于作用于外周的镇痛药。 展开更多
关键词 理气开郁法 柴郁汤 金铃子散 芍药甘草汤 痛泻要方
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Understanding the promotional effect of Au on Pt/WO_3 in hydrogenolysis of glycerol to 1,3-propanediol 被引量:5
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作者 Chaojun Yang Fan Zhang +6 位作者 Nian Lei Man Yang Fei Liu Zhili Miao Yongnan Sun Xiaochen Zhao Aiqin Wang 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2018年第8期1366-1372,共7页
Pt/Au/WO_3 bimetallic catalysts were prepared by impregnation of Pt onto preformed Au/WO_3,obtained by a hexadecyl trmethyl ammonium bromide(CTAB)-assisted one-pot synthesis method.The resulting Pt/Au/WO_3 catalysts... Pt/Au/WO_3 bimetallic catalysts were prepared by impregnation of Pt onto preformed Au/WO_3,obtained by a hexadecyl trmethyl ammonium bromide(CTAB)-assisted one-pot synthesis method.The resulting Pt/Au/WO_3 catalysts exhibited remarkable synergistic effects for selective hydrogenolysis of glycerol to 1,3-propanediol. The characterization results showed that doping of Au promoted the reduction of both Pt and W at low temperatures and uniform dispersion of Pt on the WO_3 support. Furthermore, more low-valence Pt species were produced on the WO_3 surface after introduction of Au. These changes in electronic properties resulted in enhancement of both glycerol conversion and selectivity for 1,3-propanediol. 展开更多
关键词 BimetallicPt/Au/WO3 Glycerol 1 3‐PropanediolHydrogenolysis
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Selective oxidation of glycerol with oxygen in base-free solution over N-doped-carbon-supported Sb@PtSb2 hybrid 被引量:3
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作者 Lihua Yang Tianqu He +2 位作者 Chujun Lai Ping Chen Zhaoyin Hou 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2020年第3期494-502,共9页
Selective oxidation of glycerol with molecular oxygen in base-free aqueous solutions has become a hot topic,as the rapidly increasing production of biodiesel is creating a surplus of glycerol.In this work,an N-doped-c... Selective oxidation of glycerol with molecular oxygen in base-free aqueous solutions has become a hot topic,as the rapidly increasing production of biodiesel is creating a surplus of glycerol.In this work,an N-doped-carbon-supported core-shell structured Sb@PtSb2 hybrid catalyst was prepared via a facile synthesis route,in which a mixture of glucose,melamine,and SbCl3(Sb-NC)was pyrolyzed,then impregnated with Pt by immersion in an aqueous solution of H2PtCl6,and further treated in hydrogen flow.Characterization of the catalyst products indicated that introducing SbCl3 can increase the surface area of the binary glucose+melamine pyrolyzed support(NC),and Sb@PtSb2 hybrids could be formed on the surface of an Sb-NC support during hydrogen treatment at 700℃.It was found that the Sb@PtSb2/NC catalyst was more active for the selective oxidation of glycerol in a base-free aqueous solution than Sb-free NC-supported Pt(Pt/NC).Further characterization also indicated that the promising performance of Sb@PtSb2/NC might be attributed to its enhanced oxygen activation. 展开更多
关键词 GLYCEROL Oxidation Base-free solution Sb@PtSb2 Alloy
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GEOCHEMICAL CHARACTERISTICS OF PLATINUM GROUP ELEMENTS IN JINCHUAN SUPER-LARGE SULFIDE COPPER-NICKEL DEPOSIT, JINCHANG CITY, GANSU PROVINCE, CHINA
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作者 WANG Ruiting MAO Jingwen +2 位作者 HE Ying WANG Dongsheng TANG Zhongli 《Geotectonica et Metallogenia》 2005年第2期152-163,共12页
The platinum-group element geochemistry of rocks and ores from Jinchuan super-large copper-nickel sulfide deposit is systemically studied in this paper. The Cu/Pd mean ratio of Jinchuan intrusion is lower than that of... The platinum-group element geochemistry of rocks and ores from Jinchuan super-large copper-nickel sulfide deposit is systemically studied in this paper. The Cu/Pd mean ratio of Jinchuan intrusion is lower than that of original mantle magma, which indicates that these ultrabasic rocks were crystallized from magma that lost Pd in the form of melting segregation of sulfides. The PGE of the rocks show trend of partial melting, similar to that of mantle peridotite, which shows that magma formation occurs during rock-forming and ore-forming processes. The chondrite normalized PGE patterns of the rocks and ores are well related to each other, which signifies the signatures of multi-episode magmatic intrusion, melting and differentiation in the formation processes of rocks and ores. In addition, analyses about the relation between PGE and S, and study on Re-Os isotopes indicate that few contamination of the crustal substances occurred during the magmatic intrusion and the formation of deposit. However, contamination by crustal substances helps to supply part of the S for the enrichment of PGE. Meanwhile, the hydrothermal process is also advantageous for the enrichment of PGE, especially lbr Pt and Pd, due to deep melting segregation. The characteristic parameters (such as Pt/(Pt+Pd), (Pt+Pd)/(Ru+Ir+Os), Pd/Ir, Cu/(Ni+Cu), and so on.) for platinum-group elements for Jinchuan sulfide copper-nickel deposit show the same features as those for sulfide copper-nickel deposit related to basic magma, which also illustrates its original magma property representative of Mg-high tholeiite. Therefore, it is the marie (not ultramafic) magma that resulted in the formation of the superlarge sulfide copper-nickel deposit enriched in Cu and PGE. To sum up, the geochemical characteristics of platinum-group elements in rocks and ores from Jinchuan copper-nickel sulfide deposit are constrained by the continental rift tectonic environment, the parent magma features, the enriched mantel magma source, the complex metallogenesis and PGE geochemical signatures, and this would be rather significant for the study about the genetic mechanism of copper-nickel sulfide deposits. 展开更多
关键词 platinum group elements copper-nickel sulfide deposit geochemical feature Jinchuan
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Alterations of glutathione S-transferase and matrix metalloproteinase-9 expressions are early events in esophageal carcinogenesis 被引量:37
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作者 Laszlo Herszenyi Istvan Hritz +4 位作者 Istvan Pregun Ferenc Sipos Mark Juhasz Bela Molnar Zsolt Tulassay 《World Journal of Gastroenterology》 SCIE CAS CSCD 2007年第5期676-682,共7页
AIM: To investigate the role of glutathione S-transferase (GST) and matrix metalloproteinase-9 (MMP-9) expres-sions in the development and progression of reflux es-ophagitis-Barrett’s metaplasia-dysplasia-adenocarcin... AIM: To investigate the role of glutathione S-transferase (GST) and matrix metalloproteinase-9 (MMP-9) expres-sions in the development and progression of reflux es-ophagitis-Barrett’s metaplasia-dysplasia-adenocarcinoma sequence in the esophagus.METHODS: GST and MMP-9 expressions were analyzed in 51 paraffin-embedded tissue samples by immunohisto-chemistry including patients with reflux esophagitis (n = 7), Barrett’s metaplasia (n = 14), Barrett and esophagi-tis (n = 8), Barrett and dysplasia (n = 7), esophageal adenocarcinoma (n = 8) and a control group without any histological changes (n = 7). Immunostaining was determined semiquantitatively. Statistical analysis with one-way ANOVA, LSD test and correlation analysis were performed. P value of < 0.05 was considered significant.RESULTS: GST expression was significantly higher while MMP-9 expression was significantly lower in control group compared to Barrett’s metaplasia and the other groups. No major changes were observed between Bar-rett, esophagitis, and Barrett and concomitant esophagi-tis. Barrett and concomitant dysplasia, and adenocarci-noma revealed a significant lower expression of GST and higher levels of MMP-9 compared to all other groups. Adenocarcinoma showed almost no expression of GST and significantly higher levels of MMP-9 than Barrett and concomitant dysplasia. Alterations of GST and MMP-9 were inversely correlated (r = - 0.82).CONCLUSION: Decreased GST and increased ex-pression of MMP-9 in Barrett’s metaplasia-dysplasia-adenocarcinoma sequence as compared to normal tissue suggest their association with esophageal tumorigenesis. Loss of GST and gain of MMP-9 in Barrett with dyspla-sia compared to non-dysplastic metaplasia indicate that these alterations may be early events in carcinogenesis. Quantification of these parameters in Barrett’s esopha-gus might be useful to identify patients at higher risk for progression to cancer. 展开更多
关键词 Glutathione S-transferase Matrix metallo-proteinase-9 Barrett's metaplasia ESOPHAGUS Adenocarcinoma DYSPLASIA
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Brownfield Phytoremediation of Heavy Metals Using Brassica and Salix Supplemented with EDTA: Results of the First Growing Season
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作者 F.E. Pitre T.I. Teodorescu M. Labrecque 《Journal of Environmental Science and Engineering》 2010年第9期51-59,共9页
A phytoremediation field trial was established on a site contaminated with Cu, Pb and Zn in the City of Montreal (Canada) using Brassicajuncea, Salix miyabeana and Salix viminalis. The study compared metal content i... A phytoremediation field trial was established on a site contaminated with Cu, Pb and Zn in the City of Montreal (Canada) using Brassicajuncea, Salix miyabeana and Salix viminalis. The study compared metal content in plant tissues of each species at the end of the first growing season and assessed the effects of EDTA as a chelating agent to increase availability of metals for the plants. Brassicajuncea accumulated more metals (Cu, Pb and Zn) in aerial parts than in roots, whereas the willows accumulated Cu and Pb mainly in roots and Zn in aerial parts (mostly in leaves). EDTA increased metal transfer in Brassica but had no effect on the two willow species. After one growing season, the total biomass yield of Brassica juncea was higher than that of the willows, and consequently, total metal accumulation by the plants was also greater. However, the high yield of willows over several years may make them more successful in brownfield phytoremediation. 展开更多
关键词 Soil METALS PHYTOREMEDIATION WILLOWS MUSTARD bioaccumulation metals.
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Effect of Benzo[a]pyrene on Detoxification and the Activity of Antioxidant Enzymes of Marine Microalgae 被引量:4
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作者 SHEN Chen MIAO Jingjing +1 位作者 LI Yun PAN Luqing 《Journal of Ocean University of China》 SCIE CAS 2016年第2期303-310,共8页
The objective of this study was to examine the effect ofbenzo[a]pyrene (BaP) on the detoxification and antioxidant systems of two microalgae, Isochrysis zhanjiangensis and Platymonas subcordiformis. In our study, th... The objective of this study was to examine the effect ofbenzo[a]pyrene (BaP) on the detoxification and antioxidant systems of two microalgae, Isochrysis zhanjiangensis and Platymonas subcordiformis. In our study, these two algae were exposed to BaP for 4 days at three different concentrations including 0.5 μg L-1 (low), 3 μg L-1 (mid) and 18 μg L-1 (high). The activity of detoxi- fication enzymes, ethoxyresorufin O-deethylase (EROD) and glutathione S-transferase (GST) increased in P subcordiformis in all BaP-treated groups. In 1. zhanjiangensis, the activity of these two enzymes increased at the beginning of exposure, and then de- creased in the groups treated with mid- and high BaP. The activity of antioxidant enzyme superoxide dismutase (SOD) increased in/. zhanjiangensis in all BaP-treated groups, and then decreased in high BaP-treated group, while no significant change was observed in P subcordiformis. The activity of antioxidant enzyme catalase (CAT) increased in I. zhanjiangensis and P subcordiformis in all BaP- treated groups. The content of malondialdehyde (MDA) in Isochrysis zhanjiangensis increased first, and then decreased in high BaP-treated group, while no change occurred in P. subcordiformis. These results demonstrated that BaP significantly influenced the activity of detoxifying and antioxidant enzymes in microalgae. The metabolic related enzymes (EROD, GST and CAT) may serve as sensitive biomarkers of measuring the contamination level of BaP in marine water. 展开更多
关键词 BENZO[A]PYRENE marine microalga detoxifying enzyme antioxidant enzyme lipid peroxidation
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Selection of Sugar Cane Varieties with a Low Heavy Metal Accumulation Ability for the Ecological Remediation of Contaminated Farmland 被引量:2
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作者 王学礼 陈同斌 +3 位作者 雷梅 宋波 万小铭 李艳梅 《Journal of Resources and Ecology》 CSCD 2012年第4期373-378,共6页
How to best use farmland contaminated with low to moderate levels of heavy metals remains a challenge in many parts of the world. Here, we screened sugar cane varieties to identify those with a low ability to accumula... How to best use farmland contaminated with low to moderate levels of heavy metals remains a challenge in many parts of the world. Here, we screened sugar cane varieties to identify those with a low ability to accumulate heavy metals and with high tolerance. These trials were conducted in arsenic (As), lead (Pb) and Cadmium (Cd) contaminated farmland in Huanjiang County, Guangxi Zhuang Autonomous Region, China. We tested seven varieties and found that cultivar Guiyin9 had the highest cane stem yield (187.5t ha-1 ) and sugar content (27t ha-1 ). The concentrations of As, Pb and Cd in the cane juice of Guiyin9 were all below the maximum limit for contaminants in food. Because Guiyin9 showed low accumulation of the heavy metals tested here and produced a high cane stem yield, this cultivar could be planted as a valuable crop in large areas of southern China contaminated with low to moderate levels of heavy metals. 展开更多
关键词 sugar cane VARIETY SCREENING heavy metal contaminated farmland
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理气开郁法板块方对炎症介质的作用特点研究 被引量:1
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作者 孔维涵 朱爱江 +2 位作者 方步武 吴咸中 田在善 《中药药理与临床》 CAS CSCD 北大核心 2009年第5期1-3,共3页
目的:探讨理气开郁法板块方对炎症介质影响的相对特征性作用。方法:以过滤空气皮下注射和1%角叉菜胶囊内注射制备大鼠背部气囊炎症模型,用放免法检测炎性渗出液中IL-6和PGE2及血清皮质醇,用硝酸还原酶法测定渗出液中NO,预防性灌胃用药... 目的:探讨理气开郁法板块方对炎症介质影响的相对特征性作用。方法:以过滤空气皮下注射和1%角叉菜胶囊内注射制备大鼠背部气囊炎症模型,用放免法检测炎性渗出液中IL-6和PGE2及血清皮质醇,用硝酸还原酶法测定渗出液中NO,预防性灌胃用药。结果:各板块方高、中、低剂量和氢化可的松均能显著降低大鼠气囊炎症渗出液中IL-6水平,金铃子散高剂量的作用有优于氢化可的松的趋势。金铃子散高、中、低剂量、芍甘汤高、中剂量、柴郁汤中剂量及氢化可的松均能显著降低大鼠气囊炎症渗出液中PGE2水平,金铃子散和芍甘汤高、中剂量的作用与氢化可的松相当。金铃子散、芍甘汤、柴郁汤高、中、低剂量、痛泻要方中剂量和氢化可的松均能显著降低大鼠气囊炎症渗出液中NO含量,金铃子散高、中、低剂量、芍甘汤高剂量、柴郁汤中、高剂量的作用尤著。模型对照组大鼠血清皮质醇水平明显升高,氢化可的松组与芍甘汤低、中剂量组血清皮质醇明显降低。结论:金铃子散、痛泻要方降低IL-6的作用较强;金铃子散、芍甘汤降低PGE2的作用较强;金铃子散和柴郁汤降低NO的作用较强;四方中金铃子散的抗炎作用可能最强;芍甘汤的抗炎作用可能还涉及到皮质醇。 展开更多
关键词 理气开郁法 金铃子散 芍甘汤 痛泻要方 柴郁汤 白细胞介素-6 前列腺素E2 一氧化氮 皮质醇 气囊炎症模型
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Comparative Study of Antimicrobial Activity and Phytochemical Analysis of Methanolic and Aqueous Extracts of the Fruit of Emblica Officinalis against Pathogenic Bacteria 被引量:4
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作者 Avneesh Kumar Bilal Ahmd Tantry +1 位作者 Shiak Rahiman Upasna Gupta 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2011年第3期246-250,共5页
Objective:To investigate antimicrobial potential of aqueous(infusions,decoctions) and methanlic extracts(1:2 and 1:5 concentrations) of Emblica officinalis(amla) against seven pathogenic bacteria namely Staph... Objective:To investigate antimicrobial potential of aqueous(infusions,decoctions) and methanlic extracts(1:2 and 1:5 concentrations) of Emblica officinalis(amla) against seven pathogenic bacteria namely Staphylococcs aureus,Staphylococcus saprophyticus,Escherichia coli,Enterococcus faecalis,Enterococcus cloacae,Proteus vulgaris and Klebsiella pneumoniae.Methods:The well diffusion technique was employed.The minimum inhibitory concentration(MIC) was determined using micro-broth dilution methods and phytochemical screening was done as per standard procedures.Results:Aqueous infusion extract of amla exhibited potent antimicrobial activity against E.cloacae followed by E.coli.Preliminary phytochemical analysis of E.officinalis aqueous extracts(infusions and decoctions) only showed presence of tannins,saponins,flavanoids,Terpenoids and phenols.MIC of aqueous extract of E.officinalis was most active against K.pneumoniae.Whereas MIC of methanol extract of E.officinalis shows maximum activity against E.coli.Conclusion:Emblica officinalis definitely possesses potent antimicrobial activities and this can serve as an important platform for the development of inexpensive,safe and effective medicines 展开更多
关键词 Emblica officinalis INFUSION DECOCTION traditional medicine
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Molecular characterization and efficacy evaluation of a transgenic corn event for insect resistance and glyphosate tolerance 被引量:7
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作者 Miao-miao LIU Xiao-jing ZHANG +2 位作者 Yan GAO Zhi-cheng SHEN Chao-yang LIN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2018年第8期610-619,共10页
A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein CrylAb/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated.... A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein CrylAb/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein CrylAb/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostnnia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line. 展开更多
关键词 Transgenic maize Bacillus thuringiensis (Bt) Insect resistance Glyphosate tolerance
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Synthesis of biodegradable poly (methylparaben/glycine ethyl ester) phosphazene and further research of its degradation in vitro 被引量:1
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作者 HUANG XiaoBin CHEN KuiYong +3 位作者 LI Jing ZHENG YuanLi JIANG JinGui TANG XiaoZhen 《Science China Chemistry》 SCIE EI CAS 2011年第3期426-430,共5页
Polydichlorophosphazene(PDCP)was synthesized from PCl5 and NH4Cl via a one-pot approach,and then further reacted with glycine ethyl ester and methylparaben via a two-step nucleophilic substitution approach to give pol... Polydichlorophosphazene(PDCP)was synthesized from PCl5 and NH4Cl via a one-pot approach,and then further reacted with glycine ethyl ester and methylparaben via a two-step nucleophilic substitution approach to give poly(methylparaben/glycine ethyl ester)phosphazene(PMGP),which was a kind of mix-substituted biodegradable polymer.NMR and FT-IR were used to characterize its structure.A series of experiments were conducted to study the effects of the ratio of methylparaben to glycine ethyl ester and the experimental conditions to the degradability of PMGP samples.The degradability of PMGP can be adjusted by altering the ratio of methylparaben to glycine ethyl ester easily. 展开更多
关键词 POLYPHOSPHAZENES BIODEGRADABLE METHYLPARABEN glycine ethyl ester
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