以玉米纹枯病菌菌丝为材料,利用In-Fusion SMARTer TM c DNA Lib Construction Kit构建玉米纹枯病菌全长c DNA文库。文库质量鉴定结果表明,文库滴度为1.2×106pfu·m L-1,重组率为99.2%,插入片段平均长度大于1.0 kb。随机挑取40...以玉米纹枯病菌菌丝为材料,利用In-Fusion SMARTer TM c DNA Lib Construction Kit构建玉米纹枯病菌全长c DNA文库。文库质量鉴定结果表明,文库滴度为1.2×106pfu·m L-1,重组率为99.2%,插入片段平均长度大于1.0 kb。随机挑取400个白色克隆进行测序,共获得329个高质量EST序列,经聚类拼接后得到250条unique EST,包括36条contigs和214条singletons。在Gen Bank进行Blastn与Blastx同源比对,有227条EST与已知核酸或蛋白有不同程度的同源性,占全部EST的90.8%,其余23条无任何同源性,占9.2%。展开更多
The test was undertaken to reveal the antifungal activity of extracts from Clerodendrum bungei leaves against Pestalotia and Rhizoctonia solani, the results showed that optimal condition for best antifungal activity o...The test was undertaken to reveal the antifungal activity of extracts from Clerodendrum bungei leaves against Pestalotia and Rhizoctonia solani, the results showed that optimal condition for best antifungal activity of extracts against pestalotia and Rhizoctonia solani are as follows： material-liquid ratio of 1：6,75% ethanol as extracting solvent, reflux at 90℃ for 1.5 h. The substances with good dissolubility in ethanol and water solution such as organic acid, bioflavonoid and alkaloid are main antifungal bioactive substances in Clerodendrun bungei.展开更多
文摘以玉米纹枯病菌菌丝为材料,利用In-Fusion SMARTer TM c DNA Lib Construction Kit构建玉米纹枯病菌全长c DNA文库。文库质量鉴定结果表明,文库滴度为1.2×106pfu·m L-1,重组率为99.2%,插入片段平均长度大于1.0 kb。随机挑取400个白色克隆进行测序,共获得329个高质量EST序列,经聚类拼接后得到250条unique EST,包括36条contigs和214条singletons。在Gen Bank进行Blastn与Blastx同源比对,有227条EST与已知核酸或蛋白有不同程度的同源性,占全部EST的90.8%,其余23条无任何同源性,占9.2%。
基金Supported by Doctor Startup Foundation of Yibin College(2005B02)~~
文摘The test was undertaken to reveal the antifungal activity of extracts from Clerodendrum bungei leaves against Pestalotia and Rhizoctonia solani, the results showed that optimal condition for best antifungal activity of extracts against pestalotia and Rhizoctonia solani are as follows： material-liquid ratio of 1：6,75% ethanol as extracting solvent, reflux at 90℃ for 1.5 h. The substances with good dissolubility in ethanol and water solution such as organic acid, bioflavonoid and alkaloid are main antifungal bioactive substances in Clerodendrun bungei.
