动物肠道中存在着一些参与猪肠道微生物互作的基因,这些基因具有一定的宿主特异性,利用其设计分子标记能准确识别粪便污染来源。该文共采集6个物种(猪、牛、羊、鸡、鸭、鹅)的145个粪便样品,提取其DNA后利用竞争性杂交的基因片段富集方...动物肠道中存在着一些参与猪肠道微生物互作的基因,这些基因具有一定的宿主特异性,利用其设计分子标记能准确识别粪便污染来源。该文共采集6个物种(猪、牛、羊、鸡、鸭、鹅)的145个粪便样品,提取其DNA后利用竞争性杂交的基因片段富集方法(genome fragment enrichment,GFE),靶向筛选参与猪肠道微生物互作的特异性基因。经BLASTX分析发现,82%的猪特异性非冗余DNA片段存在相似序列,以拟杆菌纲(Bacteroidetes)(43.2%)、梭菌纲(Clostridia)(19.5%)、芽孢杆菌纲(Bacilli)(8.6%)相似序列为主。从蛋白质功能方面分析,61.5%的非冗余序列功能明确,有7.6%的序列与信息贮存及加工有关,12.8%的序列与细胞加工及信息传导有关,22%的序列与代谢有关,其中,碳水化合物和氨基酸的转移代谢相关序列含量最为丰富,均占总特异性序列的6.3%。研究发现,能够编码拟杆菌纲(Bacteroidetes)和梭菌纲(Clostridials)等表面蛋白、膜分泌蛋白及碳水化合物代谢蛋白的相关基因可作为猪特异性分子标记筛选的靶点。展开更多
Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the ...Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.展开更多
文摘动物肠道中存在着一些参与猪肠道微生物互作的基因,这些基因具有一定的宿主特异性,利用其设计分子标记能准确识别粪便污染来源。该文共采集6个物种(猪、牛、羊、鸡、鸭、鹅)的145个粪便样品,提取其DNA后利用竞争性杂交的基因片段富集方法(genome fragment enrichment,GFE),靶向筛选参与猪肠道微生物互作的特异性基因。经BLASTX分析发现,82%的猪特异性非冗余DNA片段存在相似序列,以拟杆菌纲(Bacteroidetes)(43.2%)、梭菌纲(Clostridia)(19.5%)、芽孢杆菌纲(Bacilli)(8.6%)相似序列为主。从蛋白质功能方面分析,61.5%的非冗余序列功能明确,有7.6%的序列与信息贮存及加工有关,12.8%的序列与细胞加工及信息传导有关,22%的序列与代谢有关,其中,碳水化合物和氨基酸的转移代谢相关序列含量最为丰富,均占总特异性序列的6.3%。研究发现,能够编码拟杆菌纲(Bacteroidetes)和梭菌纲(Clostridials)等表面蛋白、膜分泌蛋白及碳水化合物代谢蛋白的相关基因可作为猪特异性分子标记筛选的靶点。
基金the project of Scientific Research Foundation for the Returned Overseas Chinese Scholars, New Century Excellent Talents in University (No. NCET-04-0908)Changjiang Scholars and Innovative Research Team in University (No. IRT0 453) of the Chinese Ministry of EducationNational Natural Science Foundation of China (No. 30270804), Education Department and Science and Technology Department of Sichuan Province.
文摘Microsatellites or SSRs as powerful genetic markers have widely been used in genetics and evolutionary biology in common wheat. Because of the high polymorphism, newly synthesized hexaploid wheat has been used in the construction of genetic segregation population for SSR markers, However, data on the evolution of microsatellites during the polyploidization event of hexaploid wheat are limited. In this study, 66 pairs of specific to A/B genome SSR patterns among newly synthesized hexaploid wheat, the donor tetraploid wheat and Aegilops tauschii were compared. The results indicated that most SSR markers were conserved during the polyploidization events of newly synthetic hexaploid wheat, from Triticum turgidum and Ae. tauschii. Over 70% A/B genome specific SSR markers could amplify the SSR sequences from the D genome ofAe. tauschii. Most amplified fragments from Ae, tauschii were detected in synthetic hexaploid at corresponding positions with the same sizes and patterns as in its parental Ae. tauschii. This suggested that these SSR markers, specific for A/B genome in common wheat, could amplify SSR products of D genome besides A/B genome in the newly synthesized hexaploid wheat, that is, these SSR primers specific for A/B genome in common wheat were nonspecific for the A/B genome in the synthetic hexaploid wheat. In addition, one amplified Ae. tauschii product was not detected in the newly synthetic hexaploid wheat. An extra-amplified product was found in the newly synthetic hexaploid wheat. These results suggested that caution should be taken when using SSR marker to genotype newly synthetic hexaploid wheat.
