The characteristics of photosynthesis and water metabolism of Caragana microphylla Lam.,C. davazamcii Sancz. and C. korshinskii Kom. populations in different sites (117.6o-105.7o E, 44.6o-38.8o N)were studied. (1) Fro...The characteristics of photosynthesis and water metabolism of Caragana microphylla Lam.,C. davazamcii Sancz. and C. korshinskii Kom. populations in different sites (117.6o-105.7o E, 44.6o-38.8o N)were studied. (1) From the east to the west, the responses of the three species to photosyntheticallyavailable radiation (PAR) in net photosynthesis rate increased, the relative humidity of the air whichcorresponded to the occurrence of maximum photosynthesis rate decreased, and the corresponding airtemperature increased. Along the same gradient, the before-noon superiority of the photosynthesis be-came evident, and the photosynthesis rate and the light use efficiency (LUE ) increased, while the transp-iration rate decreased, thus the water use efficiency (WUE ) increased notably, and the leaf water contentdecreased gradually. From the east to the west, the plants took a water-saving strategy step by step withhigher photosynthesis rate and lower transpiration rate. These physiological changes in the plants wereadaptable to the conditions of light, temperature and humidity in the habitat of the plants, and might be thebiological foundation for the geographical transition among C. microphylla , C. davazamcii and C. korshinskii.(2) The adaptation of photosynthetic system of C. microphylla , C. davazamcii and C. korshinskii to PAR, airhumidity and temperature exhibited the interspecific continuity, which was consistent with theenvironmental gradient. In different species and different sites, the diurnal changes of net photosynthesisrate, the daily cumulative value of net photosynthesis, the diurnal changes of transpiration rate, the dailycumulative value of transpiration, the water use efficiency and the diurnal changes of leaf water contentvaried with longitudinal descent (from the east to the west). The characteristics of photosynthesis andwater metabolism indicated that the geographical transition among C. microphylla , C. davazamcii and C.korshinskii was in gradual change, and these three species formed a geographical cline.展开更多
[Objective] The aim was to provide reference for the field irrigation management of high yield and quality cultivation of strong gluten wheat.[Method]Under field conditions,the effects of irrigation times on nitrogen ...[Objective] The aim was to provide reference for the field irrigation management of high yield and quality cultivation of strong gluten wheat.[Method]Under field conditions,the effects of irrigation times on nitrogen metabolism and yield of strong gluten wheat cultivar zhengmai 9023 were studied.[Result]The results indicated that NR activity,Chlorophyll and nitrogen content in flag leaf increased with irrigation times,and the irrigation treatment had obvious advantages during middle filling stage.Grain protein content showed ＂V＂ type change with grain filling going on,and protein content decreased when irrigation times going on.There was significant difference among treatments during early stage of grain filling,and the difference became smaller in the late grain filling stage.The grain yield and protein yield increased but the protein content decreased with increasing of irrigation times.[Conclusion] Increasing irrigation times properly could improve grain yield and protein yield per unit area,but reduce the grain protein content.展开更多
[Objective] The aim was to study on construction of overall water environment in Xi＇an. [Method] The study discussed necessity and basic principles of over-all restoration and construction of ＂Chang＇an Eight Water...[Objective] The aim was to study on construction of overall water environment in Xi＇an. [Method] The study discussed necessity and basic principles of over-all restoration and construction of ＂Chang＇an Eight Water＂ from urban planning, and explored related methods from long term planning and concrete construction approaches. [Result] The study proposed that urban landscape substrates of ＂Chang＇ an Eight Water＂ should be restored based on overall planning about urban water environment, change of linear metabolism, and construction of drainage circulation system. Furthermore, self-circulation and purification of rainfall and sewage could be made use of there in view of present urban water environment. In addition, urban ＂metabolism＂ should be restored progressively to improve urban environment with consideration of water catchment, storage, and diversion. [Conclusion] We should take the initiative to catch the special opportunity to build Xi＇an into an international metropolis with overall planning, designing and implementation, which will be conducive to reconstruction of water system and pleasant surroundings, and to presentation of urban ecosystem and context in ＂Chang＇an Eight Water＂, providing many opportunities for urban development.展开更多
AIMS To investigate the relationship between the liver function- al impairment and sodium and water retention. METHODS Acute liver damage model was established with carbon tetrachloride (CCl_4) administration to male ...AIMS To investigate the relationship between the liver function- al impairment and sodium and water retention. METHODS Acute liver damage model was established with carbon tetrachloride (CCl_4) administration to male Sprague-Daw- ley rats. Twenty-four and 48 hours later after CCl_4 administration, the excretion of acute sodium and water load was examined,and 24 hours later after normal saline administration,the excretion of acute sodium and water load was examined in control group. The concentration of plasma caffeine was analysed with high pressure liquid chromatograph (HPLC). The half life time of plasma caffein (Caft 1/2) sewed as a quantitative index of hepatic function. Plasma ALT was measured with Reitman method. The hepatic tis- sue was sectioned in the same site for water content measurement and pathological observation. The serumal and urinary sodium was measured with flame photometry. RESULTS Twenty-four hours later after CCl_4 administration, plasma alanine aminotransferase (ALT,n=6,37.5±12.6→ 189.4±34.4U,P<0.01) and water content of hepatic tissue (n =6,70.0%±1.1%→73.0%±1.0%,P<0.01) rose significantly,Caft 1/2 was prolonged significantly (94.9±18.9→ 326.4±85.8 minutes,P<0.01 ). The renal function of excretion of acute salt and water load declined obviously (n=6,Na^+: 92.4%±14.1%→50.1%±13.1%,P<0.01;H_2O:86.3%± 14.3%→42.1%±8.8%,P<0.01). Forty-eight hours later, the indexes above somewhat recovered,but were still markedly different from those of the control. Furthermore,the relationships betweenCaft 1/2 andALT (r=0.752,P<0.01),and between Caft 1/2 and excretory rate of sodium (r=-0.634,P<0.05) and water were still significant (r=-0. 612,P<0.01). CONCLUSIONS Caft 1/2 is a good index to assess the degree of hepatic damage. The hepatic dysfunction may be a factor caus- ing the renal excretory impairment to the acute sodium and water load.展开更多
[Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly sus...[Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly susceptible to disease as experimental material, the changes of catalase(CAT), and superoxide dismutase(SOD) and MDA activities in rice treated by BABA were investigated. [Result] In rice plants treated by BABA, the activities of CAT and SOD increased, meanwhile the MDA content also rose to some extent, resulting in the disease resistance to rice blast. [Conclusion] By influencing reactive oxygen metabolism, BABA endows rice plants with resistance to rice blast. BABA is safe to environment and has highly resistance-inducing capacity, it could be generalized in production.展开更多
The routine metabolic rate in southern catfish juvenile(24.3-250.8 g)fed with saccharine feed(CHO)was measured in this study.Fish were fed with iso-nitrogenous(40 % crude protein)and iso-lipidic(10% crude lipid)experi...The routine metabolic rate in southern catfish juvenile(24.3-250.8 g)fed with saccharine feed(CHO)was measured in this study.Fish were fed with iso-nitrogenous(40 % crude protein)and iso-lipidic(10% crude lipid)experimental feed containing 0(control feed),15%,30% of carbohydrate level.The routine metabolic rates of 15 % and 30% dietary carbohydrate group were significantly higher than that of 0% dietary carbohydrate group.The relationship between body mass(Wt)and routine metabolic rate(Rr)of southern catfishfed with different test feed could be described as:(1)0% CHO:ln(Rr)=0.986 ln(Wt)+1.419 r2=0.922,n=25,P<0.001;(2)15% CHO:ln(Rr)=0.912 ln(Wt)+1.741 r2=0.966,n=21,P<0.001;(3)30% CHO:ln(Rr)=0.762 ln(Wt)+2.378 r2=0.958,n=21,P<0.001.The intercept was increased and mass coefficient was decreased with the increase of carbohydrate level in feed.It could be concluded that southern Silurus meridionalis had poor metabolic capacity which led the increase of routine metabolic rate of fish with small size.But with the increase of body size southern Silurus meridionalis might had a better adaptation to high carbohydrate concentration.展开更多
In this study, the pot experiment was used to explore the differences of activity of key enzymes involved in N metabolism and NPK accumulation under Cd stress during the til iering stage of differen varieties of rice....In this study, the pot experiment was used to explore the differences of activity of key enzymes involved in N metabolism and NPK accumulation under Cd stress during the til iering stage of differen varieties of rice. The results showed that：Cd stress could increase the NPK concentration of different rice type in the til ering stage, while Shen-Liangyou 5867,Yongyou 5550 and Wu-Yunjing 27 showed the highest amplification respectively. Morever, Cd stress can also contribute to the ac-tivity of NR,GS,GOGAT increasing.A s for NR,the Cd stress significantly contribute to NR activity increasing of Huang-Huazhan and Yongyou 538 but is not significant for Wu-Yunjing 27, Shen-Liangyou 5867 and Yongyou 5550, however, the difference among them is not obvious.However, for the activity of GS , Cd stress promote the GS activity. Huang-Huazhan and Wu-Yunjing 27 with low activity in Cd normal level are the most sensitive. Meanwhile the difference between two treatment is the most significant. To the contrary, restrain the GS activity of Shen-Liangyou 5867, Yongy-ou 5550 and the difference is not significant. And under Cd stress, either difference reached significant in GS activity. Cd stress also improve the activity of GOGAT, Wu-Yunjing 27 showed the highest inprovement which showed the lowest GOGAT activity under Cd normal level. Cd stress on rice growth and development of ad-verse, make its lower seed setting rate, 1 000 grain weight decreased, resulting in different degrees of reduction of output of rice.展开更多
The nuclear receptor PPARs are fundamentally important for energy homeostasis. Through their distinct yet overlapping functions and tissue distribution, the PPARs regulate many aspects of energy metabolism at the tran...The nuclear receptor PPARs are fundamentally important for energy homeostasis. Through their distinct yet overlapping functions and tissue distribution, the PPARs regulate many aspects of energy metabolism at the transcriptional level. Functional impairment or dysregulation of these receptors leads to a variety of metabolic diseases, while their ligands offer many metabolic benefits. Studies of these receptors have advanced our knowledge of the transcriptional basis of energy metabolism and helped us understand the pathogenic mechanisms of metabolic syndrome.展开更多
2-Methylisoborneol(2-MIB) is a cyanobacterial metabolite that is responsible for many taste and odor(T&O) complaints related to the aesthetics of drinking water and poses a problem for water authorities because it...2-Methylisoborneol(2-MIB) is a cyanobacterial metabolite that is responsible for many taste and odor(T&O) complaints related to the aesthetics of drinking water and poses a problem for water authorities because it is recalcitrant during conventional water treatment. A numerical model was developed to simulate 2-MIB in the Qingcaosha Reservoir, an estuarine drinking water resource in the Changjiang estuary with known 2-MIB episodes. The objective of this study was to numerically simulate the generation and release processes of 2-MIB in the reservoir and to provide useful information for better management of drinking water resources experiencing T&O problems caused by cyanobacteria. The simulation results from 2009 to 2013 showed that the simulated 2-MIB concentrations corresponded well to the observational data. 2-MIB was released mainly during periods of low dissolved oxygen(DO) levels with an adequate potential sediment source. The temporal and spatial variations in nutrients, chlorophyll-a(Chl-a), Cyanophyta and 2-MIB were presented and analyzed during 2009 to 2013. According to the study results, high-concentration areas and peak levels of 2-MIB can be controlled by inhibiting algal growth and increasing oxygen levels in the water, which can be achieved via adequate water exchange and oxygen exposure in the reservoir, respectively.展开更多
Turbot Scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fis...Turbot Scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fish infections. However, studies concerning the pharmacokinetics of enrofloxacin in turbot are limited. In this study, the pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin, were investigated in the turbot following intravenous and oral administration at 10 mg enrofloxacin/kg body weight, at 16℃ and 10℃ water temperatures. The concentrations of enrofloxacin and ciprofloxacin in the main tissues （plasma, muscle, liver and kidney） were detected by HPLC. The results show that the plasma concentration-time data for enrofloxacin were best described as a two-compartment open model after intravenous and oral administration. Three pharmacokinetic equations were established between the concentrations and temperatures. The kinetic profile of enrofloxacin was temperature dependent. The absorption half-life of enrofloxacin was 1.99 h and 2.17 h after oral administration, whereas the elimination half-life of the drug was 98.63 h and 136.59 h at 16℃ and 10℃, respectively. The peak concentration of enrofloxacin in plasma and tissues was higher at 16℃ than that at 10℃, and the peak plasma concentration time in the liver was the shortest at both temperatures among those of other tissues. The plasma ℃/MIC ratio varied between 11.08 and 5 540.00 at 16℃; and between 7.92 and 3 960.00 at 10℃. The AUC/MIC ratio was 467.82-280 690.00 at 16℃, and 359.48-215 690.00 at 10℃. These ratios indicate that it is possible to obtain therapeutic efficacy. Very low levels of ciprofloxacin were detected. The AUC ratios of ciprofloxacin and enrofloxacin in plasma suggest that plasma ciprofloxacin might play a minor role in enrofloxacin treatment for turbot.展开更多
Salicylate is a type of pollutant widely and persistently existed in environ- ment. Microbiological degradation of salicylate as one kind of biological remediation methods has the advantages of low cost, good effect a...Salicylate is a type of pollutant widely and persistently existed in environ- ment. Microbiological degradation of salicylate as one kind of biological remediation methods has the advantages of low cost, good effect and no secondary pollution, and also attracts wide attention both at home and abroad. Currently, many salicy- late-degrading bacteria are isolated and screened, while the molecular mechanism of salicylate metabolism is also worth deep research. The research about molecular mechanism of microorganism degradation was overviewed, various degrading path- ways including catechol meta-cleavage pathway and gentisate pathway were intro- duced, and the latest progress in regulating genes of salicylate degradation was summarized.展开更多
Ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine contaminants.We investigated the influence of dilution to 75% and 50% of normal seawater salinity(...Ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine contaminants.We investigated the influence of dilution to 75% and 50% of normal seawater salinity(31.1) on the responses of the digestive gland of R.philippinarum to arsenic exposure(20 μg/L),using nuclear magnetic resonance(NMR)-based metabolomics.After acute arsenic exposure for 48 h,salinity-dependent differential metabolic responses were detected.In normal seawater,arsenic exposure increased the concentrations of branched-chain amino acids,and of threonine,proline,phosphocholine and adenosine,and it decreased the levels of alanine,hypotaurine,glucose,glycogen and ATP in the digestive glands.Differential changes in metabolic biomarkers observed at lower salinity(～23.3) included elevation of succinate,taurine and ATP,and depletion of branched-chain amino acids,threonine and glutamine.Unique effects of arsenic at the lowest salinity(～15.6) included down-regulation of glutamate,succinate and ADP,and up-regulation of phosphocholine.We conclude that salinity influences the metabolic responses of this clam to arsenic.展开更多
By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at a...By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.展开更多
Non-specific abdominal complaints are a considerable problem worldwide. Many patients are affected and many differential diagnoses have to be considered. Among these, carbohydrate malabsorption seems to play an import...Non-specific abdominal complaints are a considerable problem worldwide. Many patients are affected and many differential diagnoses have to be considered. Among these, carbohydrate malabsorption seems to play an important role. However, so far, only incomplete absorption of lactose is broadly accepted, while the malabsorption of fructose and sorbitol is still underestimated, although in many parts of the world it is much more frequent. Despite the success of dietary interventions in many patients, there are still a lot of unanswered questions that make further investigations展开更多
Objective The sex hormone and the corresponding receptor may play some roles in the development of the metabolic syndrome （MS） in the elderly men. This study was designed to examine the relationship of level of the ...Objective The sex hormone and the corresponding receptor may play some roles in the development of the metabolic syndrome （MS） in the elderly men. This study was designed to examine the relationship of level of the sex hormone and androgen receptor with MS in elderly men, thus to investigate the possible pathogenesis of MS. Methods This cross sectional study enrolled 587 elderly men, including 400 healthy controlls aged 62-92 years and 187 MS patients aged 60-87 years in Wan Shou Lu area of Beijing city. Dehydroepiandrosterone sulfate （DHAE-S）, total testosterone （TT）, sex hormone binding globulin （SHBG）, free testosterone （FT）, follicle-stimulating hormone （FSH）,Estradiol （E2）,luteinizing hormone（LH） and androgen receptor （AR） in blood were tested. Statistical analyses included the comparison analysis of variables and independent variables, correlation analysis using multi-factor linear regression, and multiple logistic regression analysis. Results DHAE-S, TT, SHBG, FT and AR fluorescence intensity in healthy control group were higher than those in MS group, however, FSH and E2 levels were lower in healthy group. Age was negatively correlated with diastolic blood pressure （DBP） and FT, but positively correlated with systolic blood pressure （SBP） and E2. AR fluorescence intensity was negatively correlated with SBP and LH. The logistic regression equation showed the negative correlation between DHEA-S, SHBG and the development of MS. Conclusions There are low levels of DHEA-S, TT, SHBG, FT and AR in the elderly patients with MS. On the contrary, FSH and E2 concentration are higher. It can be suggested that low levels of DHEA-S and SHBG may be the potential risk factors of MS in elderly men.展开更多
Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coast...Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coastal water(Pearl River Estuary), South China Sea, at 12 sites(S1–S12) were explored by community-level physiological profiling(CLPP) with BIOLOG Eco-plate and denaturing gradient gel electrophoresis(DGGE). Our results showed that the core mariculture area(S6, S7 and S8) and the sites associating with human activity and sewage discharge(S11 and S12) had higher microbial metabolic capability and bacterial community diversity than others(S1–5, S9–10). Especially, the diversity index of S11 and S12 calculated from both CLPP and DGGE data(H >3.2) was higher than that of others as sewage discharge may increase water nitrogen and phosphorus nutrient. The bacterial community structure of S6, S8, S11 and S12 was greatly influenced by total phosphorous, salinity and total nitrogen. Based on DGGE fingerprinting, proteobacteria, especially γ- and α-proteobacteria, were found dominant at all sites. In conclusion, the aquaculture area and wharf had high microbial metabolic capability. The structure and composition of bacterial community were closely related to the level of phosphorus, salinity and nitrogen.展开更多
文摘The characteristics of photosynthesis and water metabolism of Caragana microphylla Lam.,C. davazamcii Sancz. and C. korshinskii Kom. populations in different sites (117.6o-105.7o E, 44.6o-38.8o N)were studied. (1) From the east to the west, the responses of the three species to photosyntheticallyavailable radiation (PAR) in net photosynthesis rate increased, the relative humidity of the air whichcorresponded to the occurrence of maximum photosynthesis rate decreased, and the corresponding airtemperature increased. Along the same gradient, the before-noon superiority of the photosynthesis be-came evident, and the photosynthesis rate and the light use efficiency (LUE ) increased, while the transp-iration rate decreased, thus the water use efficiency (WUE ) increased notably, and the leaf water contentdecreased gradually. From the east to the west, the plants took a water-saving strategy step by step withhigher photosynthesis rate and lower transpiration rate. These physiological changes in the plants wereadaptable to the conditions of light, temperature and humidity in the habitat of the plants, and might be thebiological foundation for the geographical transition among C. microphylla , C. davazamcii and C. korshinskii.(2) The adaptation of photosynthetic system of C. microphylla , C. davazamcii and C. korshinskii to PAR, airhumidity and temperature exhibited the interspecific continuity, which was consistent with theenvironmental gradient. In different species and different sites, the diurnal changes of net photosynthesisrate, the daily cumulative value of net photosynthesis, the diurnal changes of transpiration rate, the dailycumulative value of transpiration, the water use efficiency and the diurnal changes of leaf water contentvaried with longitudinal descent (from the east to the west). The characteristics of photosynthesis andwater metabolism indicated that the geographical transition among C. microphylla , C. davazamcii and C.korshinskii was in gradual change, and these three species formed a geographical cline.
基金Support by National Key Technology R &D Program in the 11~(th) Five Year Plan of China(2006BAD02A07)~~
文摘[Objective] The aim was to provide reference for the field irrigation management of high yield and quality cultivation of strong gluten wheat.[Method]Under field conditions,the effects of irrigation times on nitrogen metabolism and yield of strong gluten wheat cultivar zhengmai 9023 were studied.[Result]The results indicated that NR activity,Chlorophyll and nitrogen content in flag leaf increased with irrigation times,and the irrigation treatment had obvious advantages during middle filling stage.Grain protein content showed ＂V＂ type change with grain filling going on,and protein content decreased when irrigation times going on.There was significant difference among treatments during early stage of grain filling,and the difference became smaller in the late grain filling stage.The grain yield and protein yield increased but the protein content decreased with increasing of irrigation times.[Conclusion] Increasing irrigation times properly could improve grain yield and protein yield per unit area,but reduce the grain protein content.
文摘[Objective] The aim was to study on construction of overall water environment in Xi＇an. [Method] The study discussed necessity and basic principles of over-all restoration and construction of ＂Chang＇an Eight Water＂ from urban planning, and explored related methods from long term planning and concrete construction approaches. [Result] The study proposed that urban landscape substrates of ＂Chang＇ an Eight Water＂ should be restored based on overall planning about urban water environment, change of linear metabolism, and construction of drainage circulation system. Furthermore, self-circulation and purification of rainfall and sewage could be made use of there in view of present urban water environment. In addition, urban ＂metabolism＂ should be restored progressively to improve urban environment with consideration of water catchment, storage, and diversion. [Conclusion] We should take the initiative to catch the special opportunity to build Xi＇an into an international metropolis with overall planning, designing and implementation, which will be conducive to reconstruction of water system and pleasant surroundings, and to presentation of urban ecosystem and context in ＂Chang＇an Eight Water＂, providing many opportunities for urban development.
文摘AIMS To investigate the relationship between the liver function- al impairment and sodium and water retention. METHODS Acute liver damage model was established with carbon tetrachloride (CCl_4) administration to male Sprague-Daw- ley rats. Twenty-four and 48 hours later after CCl_4 administration, the excretion of acute sodium and water load was examined,and 24 hours later after normal saline administration,the excretion of acute sodium and water load was examined in control group. The concentration of plasma caffeine was analysed with high pressure liquid chromatograph (HPLC). The half life time of plasma caffein (Caft 1/2) sewed as a quantitative index of hepatic function. Plasma ALT was measured with Reitman method. The hepatic tis- sue was sectioned in the same site for water content measurement and pathological observation. The serumal and urinary sodium was measured with flame photometry. RESULTS Twenty-four hours later after CCl_4 administration, plasma alanine aminotransferase (ALT,n=6,37.5±12.6→ 189.4±34.4U,P<0.01) and water content of hepatic tissue (n =6,70.0%±1.1%→73.0%±1.0%,P<0.01) rose significantly,Caft 1/2 was prolonged significantly (94.9±18.9→ 326.4±85.8 minutes,P<0.01 ). The renal function of excretion of acute salt and water load declined obviously (n=6,Na^+: 92.4%±14.1%→50.1%±13.1%,P<0.01;H_2O:86.3%± 14.3%→42.1%±8.8%,P<0.01). Forty-eight hours later, the indexes above somewhat recovered,but were still markedly different from those of the control. Furthermore,the relationships betweenCaft 1/2 andALT (r=0.752,P<0.01),and between Caft 1/2 and excretory rate of sodium (r=-0.634,P<0.05) and water were still significant (r=-0. 612,P<0.01). CONCLUSIONS Caft 1/2 is a good index to assess the degree of hepatic damage. The hepatic dysfunction may be a factor caus- ing the renal excretory impairment to the acute sodium and water load.
基金Supported by National Key Technology R&D Program During the Eleventh Five Year Plan (2006BAD08A04)Innovation Project(20076020)~~
文摘[Objective] This study was to understand the effects of β-Amino butyric acid(abbreviated as BABA) induced rice blast resistance on reactive oxygen metabolism. [Method] Using the cultivar Chaochan 2 that is highly susceptible to disease as experimental material, the changes of catalase(CAT), and superoxide dismutase(SOD) and MDA activities in rice treated by BABA were investigated. [Result] In rice plants treated by BABA, the activities of CAT and SOD increased, meanwhile the MDA content also rose to some extent, resulting in the disease resistance to rice blast. [Conclusion] By influencing reactive oxygen metabolism, BABA endows rice plants with resistance to rice blast. BABA is safe to environment and has highly resistance-inducing capacity, it could be generalized in production.
基金Supported by the National Natural Science Foundation of China(30371121)~~
文摘The routine metabolic rate in southern catfish juvenile(24.3-250.8 g)fed with saccharine feed(CHO)was measured in this study.Fish were fed with iso-nitrogenous(40 % crude protein)and iso-lipidic(10% crude lipid)experimental feed containing 0(control feed),15%,30% of carbohydrate level.The routine metabolic rates of 15 % and 30% dietary carbohydrate group were significantly higher than that of 0% dietary carbohydrate group.The relationship between body mass(Wt)and routine metabolic rate(Rr)of southern catfishfed with different test feed could be described as:(1)0% CHO:ln(Rr)=0.986 ln(Wt)+1.419 r2=0.922,n=25,P<0.001;(2)15% CHO:ln(Rr)=0.912 ln(Wt)+1.741 r2=0.966,n=21,P<0.001;(3)30% CHO:ln(Rr)=0.762 ln(Wt)+2.378 r2=0.958,n=21,P<0.001.The intercept was increased and mass coefficient was decreased with the increase of carbohydrate level in feed.It could be concluded that southern Silurus meridionalis had poor metabolic capacity which led the increase of routine metabolic rate of fish with small size.But with the increase of body size southern Silurus meridionalis might had a better adaptation to high carbohydrate concentration.
基金Supported by "Twelfth five-year" National Science and Technology Support Project(2012BAD15B04)Open Fund Project of Innovation Platform of Hunan Province Colleges and Universities(13K061)Natural Science Fund Project of Hunan Province(12JJ6016)~~
文摘In this study, the pot experiment was used to explore the differences of activity of key enzymes involved in N metabolism and NPK accumulation under Cd stress during the til iering stage of differen varieties of rice. The results showed that：Cd stress could increase the NPK concentration of different rice type in the til ering stage, while Shen-Liangyou 5867,Yongyou 5550 and Wu-Yunjing 27 showed the highest amplification respectively. Morever, Cd stress can also contribute to the ac-tivity of NR,GS,GOGAT increasing.A s for NR,the Cd stress significantly contribute to NR activity increasing of Huang-Huazhan and Yongyou 538 but is not significant for Wu-Yunjing 27, Shen-Liangyou 5867 and Yongyou 5550, however, the difference among them is not obvious.However, for the activity of GS , Cd stress promote the GS activity. Huang-Huazhan and Wu-Yunjing 27 with low activity in Cd normal level are the most sensitive. Meanwhile the difference between two treatment is the most significant. To the contrary, restrain the GS activity of Shen-Liangyou 5867, Yongy-ou 5550 and the difference is not significant. And under Cd stress, either difference reached significant in GS activity. Cd stress also improve the activity of GOGAT, Wu-Yunjing 27 showed the highest inprovement which showed the lowest GOGAT activity under Cd normal level. Cd stress on rice growth and development of ad-verse, make its lower seed setting rate, 1 000 grain weight decreased, resulting in different degrees of reduction of output of rice.
文摘The nuclear receptor PPARs are fundamentally important for energy homeostasis. Through their distinct yet overlapping functions and tissue distribution, the PPARs regulate many aspects of energy metabolism at the transcriptional level. Functional impairment or dysregulation of these receptors leads to a variety of metabolic diseases, while their ligands offer many metabolic benefits. Studies of these receptors have advanced our knowledge of the transcriptional basis of energy metabolism and helped us understand the pathogenic mechanisms of metabolic syndrome.
基金Supported by the National Key R&D Program of China(No.2017YFC0405400)the Shanghai Municipal Science and Technology Commission,China(Nos.12231201603,15YF1409900)the Hangzhou Science and Technology Guidance Project in 2016(No.20163501Y54)
文摘2-Methylisoborneol(2-MIB) is a cyanobacterial metabolite that is responsible for many taste and odor(T&O) complaints related to the aesthetics of drinking water and poses a problem for water authorities because it is recalcitrant during conventional water treatment. A numerical model was developed to simulate 2-MIB in the Qingcaosha Reservoir, an estuarine drinking water resource in the Changjiang estuary with known 2-MIB episodes. The objective of this study was to numerically simulate the generation and release processes of 2-MIB in the reservoir and to provide useful information for better management of drinking water resources experiencing T&O problems caused by cyanobacteria. The simulation results from 2009 to 2013 showed that the simulated 2-MIB concentrations corresponded well to the observational data. 2-MIB was released mainly during periods of low dissolved oxygen(DO) levels with an adequate potential sediment source. The temporal and spatial variations in nutrients, chlorophyll-a(Chl-a), Cyanophyta and 2-MIB were presented and analyzed during 2009 to 2013. According to the study results, high-concentration areas and peak levels of 2-MIB can be controlled by inhibiting algal growth and increasing oxygen levels in the water, which can be achieved via adequate water exchange and oxygen exposure in the reservoir, respectively.
基金Supported by the National Natural Science Foundation of China(Nos.30700617,31101298)the National Special Research Fund for NonProfit Sector(No.nyhyzx07-046)
文摘Turbot Scophthalmus maximus, an important aquaculture species in China, currently suffers from epizootic diseases because of high density aquaculture. Enrofloxacin has been used to treat various systemic bacterial fish infections. However, studies concerning the pharmacokinetics of enrofloxacin in turbot are limited. In this study, the pharmacokinetics of enrofloxacin and its metabolite ciprofloxacin, were investigated in the turbot following intravenous and oral administration at 10 mg enrofloxacin/kg body weight, at 16℃ and 10℃ water temperatures. The concentrations of enrofloxacin and ciprofloxacin in the main tissues （plasma, muscle, liver and kidney） were detected by HPLC. The results show that the plasma concentration-time data for enrofloxacin were best described as a two-compartment open model after intravenous and oral administration. Three pharmacokinetic equations were established between the concentrations and temperatures. The kinetic profile of enrofloxacin was temperature dependent. The absorption half-life of enrofloxacin was 1.99 h and 2.17 h after oral administration, whereas the elimination half-life of the drug was 98.63 h and 136.59 h at 16℃ and 10℃, respectively. The peak concentration of enrofloxacin in plasma and tissues was higher at 16℃ than that at 10℃, and the peak plasma concentration time in the liver was the shortest at both temperatures among those of other tissues. The plasma ℃/MIC ratio varied between 11.08 and 5 540.00 at 16℃; and between 7.92 and 3 960.00 at 10℃. The AUC/MIC ratio was 467.82-280 690.00 at 16℃, and 359.48-215 690.00 at 10℃. These ratios indicate that it is possible to obtain therapeutic efficacy. Very low levels of ciprofloxacin were detected. The AUC ratios of ciprofloxacin and enrofloxacin in plasma suggest that plasma ciprofloxacin might play a minor role in enrofloxacin treatment for turbot.
文摘Salicylate is a type of pollutant widely and persistently existed in environ- ment. Microbiological degradation of salicylate as one kind of biological remediation methods has the advantages of low cost, good effect and no secondary pollution, and also attracts wide attention both at home and abroad. Currently, many salicy- late-degrading bacteria are isolated and screened, while the molecular mechanism of salicylate metabolism is also worth deep research. The research about molecular mechanism of microorganism degradation was overviewed, various degrading path- ways including catechol meta-cleavage pathway and gentisate pathway were intro- duced, and the latest progress in regulating genes of salicylate degradation was summarized.
基金Supported by the National Natural Science Foundation of China(No.41106102)Natural Science Foundation of Shandong Province(No.ZR2009CZ008)the 100 Talents Program of Chinese Academy of Sciences
文摘Ruditapes philippinarum,a clam that thrives in intertidal zones of various salinities,is a useful biomonitor to marine contaminants.We investigated the influence of dilution to 75% and 50% of normal seawater salinity(31.1) on the responses of the digestive gland of R.philippinarum to arsenic exposure(20 μg/L),using nuclear magnetic resonance(NMR)-based metabolomics.After acute arsenic exposure for 48 h,salinity-dependent differential metabolic responses were detected.In normal seawater,arsenic exposure increased the concentrations of branched-chain amino acids,and of threonine,proline,phosphocholine and adenosine,and it decreased the levels of alanine,hypotaurine,glucose,glycogen and ATP in the digestive glands.Differential changes in metabolic biomarkers observed at lower salinity(～23.3) included elevation of succinate,taurine and ATP,and depletion of branched-chain amino acids,threonine and glutamine.Unique effects of arsenic at the lowest salinity(～15.6) included down-regulation of glutamate,succinate and ADP,and up-regulation of phosphocholine.We conclude that salinity influences the metabolic responses of this clam to arsenic.
基金Supported by the National Natural Science Foundation of China (No. 20076011, No. 20236040 and No. 20225619).
文摘By investigating the shear effect on submerged cultivation of a traditional Chinese medicinal herb Ganoderma lucidum, a relatively high cell concentration of 13.8 g·L-1 by dry mass was obtained in bioreactor at an impeller tip speed (ITS) of 0.51m·s-1. At an ITS of 0.51,1.02 and 1.53m·s-1, a maximal production titer of intracellular polysaccharide was 2.64, 2.20 and 2.28g·L-1 and that of ganoderic acid was 306, 299 and 273g·L-1, respectively. Under these ITSs, the maximal mean projected area of dispersed hyphae was 3.70, 2.54 and 2.13×104μm2, and that of pellets was 0.91, 0.67 and 0.55mm2, respectively. The information obtained is useful for efficient submerged cultivation of mushrooms on a large scale.
文摘Non-specific abdominal complaints are a considerable problem worldwide. Many patients are affected and many differential diagnoses have to be considered. Among these, carbohydrate malabsorption seems to play an important role. However, so far, only incomplete absorption of lactose is broadly accepted, while the malabsorption of fructose and sorbitol is still underestimated, although in many parts of the world it is much more frequent. Despite the success of dietary interventions in many patients, there are still a lot of unanswered questions that make further investigations
文摘Objective The sex hormone and the corresponding receptor may play some roles in the development of the metabolic syndrome （MS） in the elderly men. This study was designed to examine the relationship of level of the sex hormone and androgen receptor with MS in elderly men, thus to investigate the possible pathogenesis of MS. Methods This cross sectional study enrolled 587 elderly men, including 400 healthy controlls aged 62-92 years and 187 MS patients aged 60-87 years in Wan Shou Lu area of Beijing city. Dehydroepiandrosterone sulfate （DHAE-S）, total testosterone （TT）, sex hormone binding globulin （SHBG）, free testosterone （FT）, follicle-stimulating hormone （FSH）,Estradiol （E2）,luteinizing hormone（LH） and androgen receptor （AR） in blood were tested. Statistical analyses included the comparison analysis of variables and independent variables, correlation analysis using multi-factor linear regression, and multiple logistic regression analysis. Results DHAE-S, TT, SHBG, FT and AR fluorescence intensity in healthy control group were higher than those in MS group, however, FSH and E2 levels were lower in healthy group. Age was negatively correlated with diastolic blood pressure （DBP） and FT, but positively correlated with systolic blood pressure （SBP） and E2. AR fluorescence intensity was negatively correlated with SBP and LH. The logistic regression equation showed the negative correlation between DHEA-S, SHBG and the development of MS. Conclusions There are low levels of DHEA-S, TT, SHBG, FT and AR in the elderly patients with MS. On the contrary, FSH and E2 concentration are higher. It can be suggested that low levels of DHEA-S and SHBG may be the potential risk factors of MS in elderly men.
基金supported by the Support Project of Science and Technology of China (Grant No. 2012BAD18B01)Special Fund for Agro-scientific Research in the Public Interest (201403008)+1 种基金the Natural Science Foundation of China (Grant Nos. U1301235, 41173079)special scientific research funds for central non-profit institutes,South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (2014TS04)
文摘Characteristics of a microbial community are important as they indicate the status of aquatic ecosystems. In the present study, the metabolic and phylogenetic profile of the bacterioplankton community in Guishan coastal water(Pearl River Estuary), South China Sea, at 12 sites(S1–S12) were explored by community-level physiological profiling(CLPP) with BIOLOG Eco-plate and denaturing gradient gel electrophoresis(DGGE). Our results showed that the core mariculture area(S6, S7 and S8) and the sites associating with human activity and sewage discharge(S11 and S12) had higher microbial metabolic capability and bacterial community diversity than others(S1–5, S9–10). Especially, the diversity index of S11 and S12 calculated from both CLPP and DGGE data(H >3.2) was higher than that of others as sewage discharge may increase water nitrogen and phosphorus nutrient. The bacterial community structure of S6, S8, S11 and S12 was greatly influenced by total phosphorous, salinity and total nitrogen. Based on DGGE fingerprinting, proteobacteria, especially γ- and α-proteobacteria, were found dominant at all sites. In conclusion, the aquaculture area and wharf had high microbial metabolic capability. The structure and composition of bacterial community were closely related to the level of phosphorus, salinity and nitrogen.
