针对成熟油菜次生休眠种子富含多糖和多酚、老健组织部分富含RNA酶、生理活性低等特点,通过选用RNAplant plus reagent植物总RNA提取试剂,添加β-巯基乙醇抑制RNA酶活性、防止酚类物质氧化,应用醋酸钠去除多糖类物质对RNA的影响,以及缩...针对成熟油菜次生休眠种子富含多糖和多酚、老健组织部分富含RNA酶、生理活性低等特点,通过选用RNAplant plus reagent植物总RNA提取试剂,添加β-巯基乙醇抑制RNA酶活性、防止酚类物质氧化,应用醋酸钠去除多糖类物质对RNA的影响,以及缩短抽提时间等措施,建立成熟油菜次生休眠种子RNA的快速高效提取方法。结果表明:采用本方法提取的RNA D260nm/D280nm为1.92～2.05,28SrRNA和18SrRNA条带清晰而完整,且无明显降解,可以应用于后续的mRNA分离、建库和高通量转录组测序。展开更多
Aims In species with morphophysiological seed dormancy(MPD),little is known about the effects of desiccation of imbibed seeds on embryo growth and germination.We studied seed responses to dehydration in nine species w...Aims In species with morphophysiological seed dormancy(MPD),little is known about the effects of desiccation of imbibed seeds on embryo growth and germination.We studied seed responses to dehydration in nine species with different levels of MPD.Methods For each species,a control test was conducted by keeping seeds permanently hydrated and exposed to the optimal stratification-incubation sequenee to promote embryo growth.Simultaneously,tests were run in which seed stratification was interrupted for 1 month by desiccation at room temperature.Important Findings In Clematis vitalba and Ribes alpinum,with non deep simple MPD,desiccation affected neither embryo growth nor seed viability,but the desiccation led to a decrease of germinative ability in R.alpinum by 16%.The seeds of Narcissus pseudonarcissus subsp.munozii-garmendiae,with deep simple epicotyl MPD,tolerated desiccation in different embryo growth stages,but their germinative ability decreased slightly.The response of species with complex levels of MPD to desiccation was more variable:Delphinium fissum subsp.sordidum,with intermediate complex MPD,and Anthriscus sylvestris and Meum athamanticum,both with deep complex MPD,tolerated desiccation.In contrast,Ribes uva-crispa with nondeep complex MPD,Lonicera pyrenaica with intermediate complex MPD and Chaerophyllum aureum with deep complex MPD,had diminished germination ability by desiccation.Although seeds of the species with simple levels of MPD tolerated desiccation,those of some species with complex levels were also highly tolerant.Thus,desiccation did not induce secondary dormancy in late embryo growth stages.The desiccation tolerance of imbibed seeds of most of the nine species may show their adaptability to climate change in the Mediterranean region.展开更多
文摘针对成熟油菜次生休眠种子富含多糖和多酚、老健组织部分富含RNA酶、生理活性低等特点,通过选用RNAplant plus reagent植物总RNA提取试剂,添加β-巯基乙醇抑制RNA酶活性、防止酚类物质氧化,应用醋酸钠去除多糖类物质对RNA的影响,以及缩短抽提时间等措施,建立成熟油菜次生休眠种子RNA的快速高效提取方法。结果表明:采用本方法提取的RNA D260nm/D280nm为1.92～2.05,28SrRNA和18SrRNA条带清晰而完整,且无明显降解,可以应用于后续的mRNA分离、建库和高通量转录组测序。
基金supported by the local Government of Castilla-La Mancha(Regional Plan for Research and Technological Development,Regional Ministry of Education and ScienceProject:Germination ecology of 12 singular or threatened plant species with morphophysiological dormancy,PEII10-0170-1830).
文摘Aims In species with morphophysiological seed dormancy(MPD),little is known about the effects of desiccation of imbibed seeds on embryo growth and germination.We studied seed responses to dehydration in nine species with different levels of MPD.Methods For each species,a control test was conducted by keeping seeds permanently hydrated and exposed to the optimal stratification-incubation sequenee to promote embryo growth.Simultaneously,tests were run in which seed stratification was interrupted for 1 month by desiccation at room temperature.Important Findings In Clematis vitalba and Ribes alpinum,with non deep simple MPD,desiccation affected neither embryo growth nor seed viability,but the desiccation led to a decrease of germinative ability in R.alpinum by 16%.The seeds of Narcissus pseudonarcissus subsp.munozii-garmendiae,with deep simple epicotyl MPD,tolerated desiccation in different embryo growth stages,but their germinative ability decreased slightly.The response of species with complex levels of MPD to desiccation was more variable:Delphinium fissum subsp.sordidum,with intermediate complex MPD,and Anthriscus sylvestris and Meum athamanticum,both with deep complex MPD,tolerated desiccation.In contrast,Ribes uva-crispa with nondeep complex MPD,Lonicera pyrenaica with intermediate complex MPD and Chaerophyllum aureum with deep complex MPD,had diminished germination ability by desiccation.Although seeds of the species with simple levels of MPD tolerated desiccation,those of some species with complex levels were also highly tolerant.Thus,desiccation did not induce secondary dormancy in late embryo growth stages.The desiccation tolerance of imbibed seeds of most of the nine species may show their adaptability to climate change in the Mediterranean region.
