为了解不同浓度有机干扰物对含氯消毒剂杀菌效果的影响,使用牛血清白蛋白作为有机干扰物,采用悬液定量和载体定量杀菌试验方法进行了实验室观察。结果,用含30 g/L牛血清白蛋白的菌悬液进行试验:有效氯为300 mg/L时可100%杀灭大肠杆菌,...为了解不同浓度有机干扰物对含氯消毒剂杀菌效果的影响,使用牛血清白蛋白作为有机干扰物,采用悬液定量和载体定量杀菌试验方法进行了实验室观察。结果,用含30 g/L牛血清白蛋白的菌悬液进行试验:有效氯为300 mg/L时可100%杀灭大肠杆菌,需要次氯酸钠溶液作用7.5 m in;三氯异氰尿酸钠片剂和二氯异氰尿酸钠粉剂作用15 m in;以含有效氯2500 mg/L的片剂和粉剂对枯草杆菌黑色变种芽孢作用30 m in,有效氯5000 mg/L次氯酸钠水溶液作用90 m in,杀灭率均达到100%。用含3 g/L牛血清白蛋白的菌悬液进行试验:以含有效氯50 mg/L作用7.5m in,3种剂型含氯消毒剂对大肠杆菌的杀灭率均可达100%。用含有效氯1000 mg/L的片剂和粉剂作用30m in;有效氯2000 mg/L的次氯酸钠水溶液作用60 m in,对枯草杆菌黑色变种芽孢杀灭率均可达100%。用载体法试验:以有效氯300 mg/L次氯酸钠溶液作用7.5 m in,杀灭大肠杆菌100%;以有效氯2000 mg/L次氯酸钠溶液作用60 m in,对枯草杆菌黑色变种芽孢杀灭率100%。结论,在高浓度有机干扰物条件下,含氯消毒剂片剂和粉剂杀灭细菌芽孢剂量明显高于液体制剂;在低浓度有机干扰物条件下,悬液法与载体法对含氯消毒剂杀菌试验结果基本一致。展开更多
A solution culture experiment was conducted to investigate the effects of collection time and interferingions on separation and determination of low-molecular-weight organic acids in root exudates of soybeanusing the ...A solution culture experiment was conducted to investigate the effects of collection time and interferingions on separation and determination of low-molecular-weight organic acids in root exudates of soybeanusing the method for directly collecting root exudates. The suitable collection time of root exudates andthe interfering ions affecting organic acid determination were determined. The method for removing theinterfering ions was established and analyzed. The release amount of root exudates increased with theincrease of collection time from 0 to 120 min but decreased with increasing of collection time from 120 to 240min. The maximum exuding amounts of organic acids were observed in root exudates at the collection time of120 min. There was a significant difference of organic acid components between the treatments of collectiontime of 120 min and 240 min. Citric acid was found only in the treatment of 120 min collection time. NO3-was the main interfering ion in organic acid determination and had the same retention time as oxalic acid.Anion exchangs resin (SAX) properly treated by HPLC (high performance liquid chromatography) solventcould remove NO3- anion in sample solution of root exudates, thus enhancing the recoveries of organic acidsin root exudates. There was no significant effect of the chemicals added into sample solution such as H3PO4,SAX and KNO3 on the retention time of organic acids.展开更多
文摘为了解不同浓度有机干扰物对含氯消毒剂杀菌效果的影响,使用牛血清白蛋白作为有机干扰物,采用悬液定量和载体定量杀菌试验方法进行了实验室观察。结果,用含30 g/L牛血清白蛋白的菌悬液进行试验:有效氯为300 mg/L时可100%杀灭大肠杆菌,需要次氯酸钠溶液作用7.5 m in;三氯异氰尿酸钠片剂和二氯异氰尿酸钠粉剂作用15 m in;以含有效氯2500 mg/L的片剂和粉剂对枯草杆菌黑色变种芽孢作用30 m in,有效氯5000 mg/L次氯酸钠水溶液作用90 m in,杀灭率均达到100%。用含3 g/L牛血清白蛋白的菌悬液进行试验:以含有效氯50 mg/L作用7.5m in,3种剂型含氯消毒剂对大肠杆菌的杀灭率均可达100%。用含有效氯1000 mg/L的片剂和粉剂作用30m in;有效氯2000 mg/L的次氯酸钠水溶液作用60 m in,对枯草杆菌黑色变种芽孢杀灭率均可达100%。用载体法试验:以有效氯300 mg/L次氯酸钠溶液作用7.5 m in,杀灭大肠杆菌100%;以有效氯2000 mg/L次氯酸钠溶液作用60 m in,对枯草杆菌黑色变种芽孢杀灭率100%。结论,在高浓度有机干扰物条件下,含氯消毒剂片剂和粉剂杀灭细菌芽孢剂量明显高于液体制剂;在低浓度有机干扰物条件下,悬液法与载体法对含氯消毒剂杀菌试验结果基本一致。
文摘A solution culture experiment was conducted to investigate the effects of collection time and interferingions on separation and determination of low-molecular-weight organic acids in root exudates of soybeanusing the method for directly collecting root exudates. The suitable collection time of root exudates andthe interfering ions affecting organic acid determination were determined. The method for removing theinterfering ions was established and analyzed. The release amount of root exudates increased with theincrease of collection time from 0 to 120 min but decreased with increasing of collection time from 120 to 240min. The maximum exuding amounts of organic acids were observed in root exudates at the collection time of120 min. There was a significant difference of organic acid components between the treatments of collectiontime of 120 min and 240 min. Citric acid was found only in the treatment of 120 min collection time. NO3-was the main interfering ion in organic acid determination and had the same retention time as oxalic acid.Anion exchangs resin (SAX) properly treated by HPLC (high performance liquid chromatography) solventcould remove NO3- anion in sample solution of root exudates, thus enhancing the recoveries of organic acidsin root exudates. There was no significant effect of the chemicals added into sample solution such as H3PO4,SAX and KNO3 on the retention time of organic acids.
