Public Key Encryption with Keyword Search （PEKS）, an indispensable part of searchable encryption, is stock-in- trade for both protecting data and providing operability of encrypted data. So far most of PEKS schemes ...Public Key Encryption with Keyword Search （PEKS）, an indispensable part of searchable encryption, is stock-in- trade for both protecting data and providing operability of encrypted data. So far most of PEKS schemes have been established on Identity-Based Cryptography （IBC） with key escrow problem inherently. Such problem severely restricts the promotion of IBC-based Public Key Infrastructure including PEKS component. Hence, Certificateless Public Key Cryptography （CLPKC） is efficient to remove such problem. CLPKC is introduced into PEKS, and a general model of Certificateless PEKS （CLPEKS） is formalized. In addition, a practical CLPEKS scheme is constructed with security and efficiency analyses. The proposal is secure channel free, and semantically secure against adaptive chosen keyword attack and keyword guessing attack. To illustrate the superiority, massive experiments are conducted on Enron Email dataset which is famous in information retrieval field. Compared with existed constructions, CLPEKS improves the efficiency in theory and removes the key escrow problem.展开更多
The differentiation status of neuroblastoma （NB） strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of sternness and differentiation remain poorly understood. Here,...The differentiation status of neuroblastoma （NB） strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of sternness and differentiation remain poorly understood. Here, we show that plant homeodomain finger-containing protein 20 （PHF20） functions as a critical epigenetic regulator in sustaining stem cell-like phenotype of NB by using CRISPR/Casg-based targeted knockout （KO） for high-throughput screening of gene function in NB cell differentiation. The expression of PHF20 in NB was significantly associated with high aggressiveness of the tumor and poor outcomes for NB patients. Deletion of PHF20 inhibited NB cell proliferation, invasive migration, and stem ceU-Uke traits. Mechanistically, PHF20 interacts with poly（ADP-ribose） polymerase 1 （PARP1） and directly binds to promoter regions of octamer-binding transcription factor 4 （OCT4） and sex determining region Y-box 2 （SOX2） to modulate a histone mark associated with active transcription, trimethylation of lysine 4 on histone H3 protein subunit （H3K4me3）. Overexpression of OCT4 and SOX2 restored growth and progression of PHF20 KO tumor cells. Consistently, OCT4 and SOX2 protein levels in clinical NB specimens were positively correlated with PHF20 expression. Our results establish PHF20 as a key driver of NB stem cell-like properties and aggressive behaviors, with implications for prognosis and therapy.展开更多
基金This research was supported by the National Science Foundation of China for Funding Projects （61173089,61472298） and National Statistical Science Program of China（2013LZ46）.
文摘Public Key Encryption with Keyword Search （PEKS）, an indispensable part of searchable encryption, is stock-in- trade for both protecting data and providing operability of encrypted data. So far most of PEKS schemes have been established on Identity-Based Cryptography （IBC） with key escrow problem inherently. Such problem severely restricts the promotion of IBC-based Public Key Infrastructure including PEKS component. Hence, Certificateless Public Key Cryptography （CLPKC） is efficient to remove such problem. CLPKC is introduced into PEKS, and a general model of Certificateless PEKS （CLPEKS） is formalized. In addition, a practical CLPEKS scheme is constructed with security and efficiency analyses. The proposal is secure channel free, and semantically secure against adaptive chosen keyword attack and keyword guessing attack. To illustrate the superiority, massive experiments are conducted on Enron Email dataset which is famous in information retrieval field. Compared with existed constructions, CLPEKS improves the efficiency in theory and removes the key escrow problem.
基金This work was supported by grants from the National Natural Science Foundation of China （81572766 and 31771630）, the National Key Research and Development Program of China （2017YFA0103800）, Guangdong Innovative and Entrepreneurial Research Team Program （2016ZT06S029）, Guangdong Natural Science Foundation （2016A030313215 and 2016A030313238）, SYSU Young Teachers Training Program （16YKZD14） and grants （CA101795 and IU54CA210181） from U.S. National Cancer Institute, National Institutes of Health （NIH）, DOD （W81XWH-16- 1-0417）, and CPRIT （DP150099, RP170537, and RP150611）.
文摘The differentiation status of neuroblastoma （NB） strongly correlates with its clinical outcomes; however, the molecular mechanisms driving maintenance of sternness and differentiation remain poorly understood. Here, we show that plant homeodomain finger-containing protein 20 （PHF20） functions as a critical epigenetic regulator in sustaining stem cell-like phenotype of NB by using CRISPR/Casg-based targeted knockout （KO） for high-throughput screening of gene function in NB cell differentiation. The expression of PHF20 in NB was significantly associated with high aggressiveness of the tumor and poor outcomes for NB patients. Deletion of PHF20 inhibited NB cell proliferation, invasive migration, and stem ceU-Uke traits. Mechanistically, PHF20 interacts with poly（ADP-ribose） polymerase 1 （PARP1） and directly binds to promoter regions of octamer-binding transcription factor 4 （OCT4） and sex determining region Y-box 2 （SOX2） to modulate a histone mark associated with active transcription, trimethylation of lysine 4 on histone H3 protein subunit （H3K4me3）. Overexpression of OCT4 and SOX2 restored growth and progression of PHF20 KO tumor cells. Consistently, OCT4 and SOX2 protein levels in clinical NB specimens were positively correlated with PHF20 expression. Our results establish PHF20 as a key driver of NB stem cell-like properties and aggressive behaviors, with implications for prognosis and therapy.
