Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed c...Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them.Three fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST program.It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B.cinerea.展开更多
文摘Differential display reverse transcription-PCR method was utilized to analyze the differential expression genes in different Arabidopsis ecotypes after inoculating Botrytis cinerea.Total 150 differentially expressed cDNA fragments were obtained,49 cDNA fragments were found to hybridize to cDNA and 5 cDNA fragments were cloned and then sequenced among them.Three fragments displayed high homology(96.1%,99.6% and 100.0%) to phosphatidylinositol transfer-like protein IV(Sec14p protein),TAC clone K9I9 and NADH-dehydrogenase subunit 4L of Arabidopsis thaliana,while alignment with NCBI databases using BLAST program.It was speculated that these fragments might relate to disease-resistance of Arabidopsis to B.cinerea.
