[Objective] The experiment aimed to study an efficient method of Nuclei extraction of cotton and provided technical support for constructing large-insert genomic library and sequencing complete genome. [Method] The co...[Objective] The experiment aimed to study an efficient method of Nuclei extraction of cotton and provided technical support for constructing large-insert genomic library and sequencing complete genome. [Method] The cotton cotyledons germinated in dark moisture chamber for one week were chopped with a sharp sterile scalpel in a Petri dish which contained ice-cold nucleus isolation buffer (10 mmol/L MgSO4, 5 mmol/L KCl, 0.5 mmol/L HEPES, 1 mg/ml DTT, 0.25% Triton X-100 and 2% PVP40), then the nuclei were collected after selected through 100, 50 and 30 μm nylon meshes and centrifugation. Meanwhile, the tender leaves and cotyledons with different germination time in dark were treated by grinding method and sharp scalpel method. [Result] The chopping with a sharp scalpel method was very simple and rapid, which did not need grind and mercaptoethanol treatment and the successful extraction rate was 100%.[Conclusion] An efficient method of nuclei extraction of cotton with simple, high efficiency, rapid reaction and poison free were established.展开更多
[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical ev...[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively high photo- synthetic electron transport capacity and high stomatal conductance after recovery treatment, which is contributed to gas exchange and recovery of photosynthetic ca- pacity. Determination of the six indicators under the same stress condition can be adopted for rapid identification and prediction of the chilling tolerance of other cotton varieties, which provides basis for the breeding, promotion, identification and screen- ing of chilling tolerant germplasms.展开更多
In order to reveal the genetic differences and agronomic traits of Fagopy-rum tataricum_ varieties （lines） intuitively, explore good resources and avoid the blindness of parent selection during the breeding process,...In order to reveal the genetic differences and agronomic traits of Fagopy-rum tataricum_ varieties （lines） intuitively, explore good resources and avoid the blindness of parent selection during the breeding process, six primary agronomic traits of 45 F. tataricum_ varieties （lines） that came from the eleven buckwheat breeding departments across the country were analyzed with principal component analysis and cluster analysis. The results of principal component analysis showed that the six agronomic traits could be simplified into three principal components, and the cumulative contribution rate reached 83%. The results of cluster analysis showed that the 45 F. tataricum varieties （lines） were classified into four groups：high stalk, medium yield and smal grain type, medium stalk, high yield and large grain type, medium stalk, low yield and smal grain type and high stalk, medium yield and medium grain type. Among them, performance of comprehensive trait of the second type was better than that of the other types. Thus, the F. tataricum_va-rieties （lines） that were classified into the second type could be considered as good varieties （lines） or breeding materials. The genetic differences among F. tataricum_varieties （lines） had no necessary correlations with origin and geographical distance. ln addition to complementary traits and geographical distance, genetic distances （dif-ferent populations） should be taken into consideration during parent selection in cross breeding.展开更多
[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was int...[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.展开更多
[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primer...[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.展开更多
[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteris...[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteristics were identified by laboratory tests and rural promotion practices in Sichuan Province.[Result]The cocoon produced by the new variety Shuhuang No.1 was golden-yellow and radiantly beautiful.Shuhuang No.1 was strong and easy to rear,uniform in development.The male and female ones could be distinguished by their larval color and markings.The cocoon yield per 10 000 the 4th instar larvae reached 20.30 kg; the length of a cocoon filament was about 1 115.0 m,and a non-broken filament was 882.95 mm long,accounting for 79.15％ of the total length.The raw silk ratio of fresh cocoon was 18.39％,and the neatness was 91.25 point.The main economic features of Shuhuang No.1 were better than those of the control.[Conclusion] The new variety had been approved by Sichuan Silkworm Evaluation Commission.It can be promoted in parent silkworm rearing areas Sichuan,Chongqing,Yangtze River basin.展开更多
[Objective] This study aimed to analyze the differences in absorption capacity of different flue-cured tobacco cultivars for chromium （Cr）, arsenic （As）, cadmium （Cd）, lead （Pb）, mercury （Hg） and copper （Cu...[Objective] This study aimed to analyze the differences in absorption capacity of different flue-cured tobacco cultivars for chromium （Cr）, arsenic （As）, cadmium （Cd）, lead （Pb）, mercury （Hg） and copper （Cu）. [Method] With 4 flue-cured tobacco cultivars as the experimental materials, their absorption capacity for 6 kinds of heavy metals was compared by pot experiment. [Result] There were great differences in absorption capacity of different flue-cured tobacco cultivars for the six kinds of heavy metals. According to the absorption capacity of different flue-cured tobacco cultivars for the heavy metals, the six heavy metals could be classified into three categories. The first category included only Cd with average enrichment coefficient of 8.564; the second category included Hg with average enrichment coefficient of 0.753; and the third category included Cr, As, Pb and Cu with average enrichment coefficients below or equivalent to 0.114. [Conclusion] The absorption capacity of different flue-cured tobacco cultivars for heavy metals was grasped by this study, thereby providing reference data for screening of high-yielding and high-quality flue-cured tobacco cultivars.展开更多
The variation in nitrogen （N） uptake by rice has been widely studied but differences in rice root morphology that may contribute to this variation are not completely understood. Field and greenhouse experiments were...The variation in nitrogen （N） uptake by rice has been widely studied but differences in rice root morphology that may contribute to this variation are not completely understood. Field and greenhouse experiments were carried out to study N accumulation, root dry weights, total root lengths, root surface areas, and root bleeding rates of two rice cultivars, Elio with low N-use efficiency and Nanguang with high N-use efficiency. Low （1 mmol N L^-1） and high （5 mmol N L^-1） N applications were established in the greenhouse experiment, and the N rates were 0, 120, and 240 kg ha^-1 in the field experiments at Jiangning and Jiangpu farms, Nanjing, China. The results showed that the N accumulation, root dry weight, total root length, and root surface area increased with an increase in N application. At the heading stage, N accumulation in the shoots and roots of Nanguang was greater than that of Elio in the field experiments and that of Elio at 5 mmol N L^-1 in the greenhouse experiment. After the heading stage, N accumulation was higher for Nanguang at both 1 and 5 mmol N L^-1 in the greenhouse experiment. The total root length and root surface area were significantly different between the two cultivars. Over the range of the fertilizer application rates, the root lengths of Nanguang at Jiangning Farm were 49%-6170 greater at booting and 26%-39% greater at heading than those of Elio, and at Jiangpu Farm they were 22%-42% and 26%-38% greater, respectively. Nanguang had a greater root bleeding rate than Elio. It was concluded that the N-use efficiency of the two rice cultivars studied depended to a great extent on the root morphological parameters and root physiological characteristics at different growth stages.展开更多
Hangjiao Hongfeng is a new hot pepper F1 hybrid cultivar which is bred with the cytoplasmic male sterile line 11066 AH as the female parent and the restoringline 11044 CH as the male parent. Its fruit is early-mid mat...Hangjiao Hongfeng is a new hot pepper F1 hybrid cultivar which is bred with the cytoplasmic male sterile line 11066 AH as the female parent and the restoringline 11044 CH as the male parent. Its fruit is early-mid maturing and in the shape of a long finger.The immature fruit is in dark-green and mature fruit is in dark-red.The average length, width and flesh thickness are 15.7, 1.9 and 0.17 cm, respectively. The yield of dry pepper is 6 750 kg/hm^2. The average contents of Vc, soluble solid and dry matter are 1.30 mg/g, 10%, and 14% respectively. The cultivar has good resistance to pepper blight, viral disease and anthracnose. It shows strong tolerance to heat, water logging and drought. It is suitable for spring field cultivation in Gansu, Xinjiang and Inner Mongolia.展开更多
基金Supported by the National Natural Science Foundation of China(No.30170501)the State of New Varieties of GMO Cultivation Major Projects (No.2008ZX08005-003)the National High-tech Research Development Plan (No.2003AA207051)~~
文摘[Objective] The experiment aimed to study an efficient method of Nuclei extraction of cotton and provided technical support for constructing large-insert genomic library and sequencing complete genome. [Method] The cotton cotyledons germinated in dark moisture chamber for one week were chopped with a sharp sterile scalpel in a Petri dish which contained ice-cold nucleus isolation buffer (10 mmol/L MgSO4, 5 mmol/L KCl, 0.5 mmol/L HEPES, 1 mg/ml DTT, 0.25% Triton X-100 and 2% PVP40), then the nuclei were collected after selected through 100, 50 and 30 μm nylon meshes and centrifugation. Meanwhile, the tender leaves and cotyledons with different germination time in dark were treated by grinding method and sharp scalpel method. [Result] The chopping with a sharp scalpel method was very simple and rapid, which did not need grind and mercaptoethanol treatment and the successful extraction rate was 100%.[Conclusion] An efficient method of nuclei extraction of cotton with simple, high efficiency, rapid reaction and poison free were established.
基金Supported by"11thFive-Year Plan"National Science and Technology Support Program(2009BADA4B01-3)~~
文摘[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively high photo- synthetic electron transport capacity and high stomatal conductance after recovery treatment, which is contributed to gas exchange and recovery of photosynthetic ca- pacity. Determination of the six indicators under the same stress condition can be adopted for rapid identification and prediction of the chilling tolerance of other cotton varieties, which provides basis for the breeding, promotion, identification and screen- ing of chilling tolerant germplasms.
基金Supported by National Oat and Buckwheat Industrial Technology System(CARS-08-A-1-3)Breeding Project of Shanxi Academy of Agricultural Sciences during the Thirteenth Five-Year Plan Period(16yzgc035)~~
文摘In order to reveal the genetic differences and agronomic traits of Fagopy-rum tataricum_ varieties （lines） intuitively, explore good resources and avoid the blindness of parent selection during the breeding process, six primary agronomic traits of 45 F. tataricum_ varieties （lines） that came from the eleven buckwheat breeding departments across the country were analyzed with principal component analysis and cluster analysis. The results of principal component analysis showed that the six agronomic traits could be simplified into three principal components, and the cumulative contribution rate reached 83%. The results of cluster analysis showed that the 45 F. tataricum varieties （lines） were classified into four groups：high stalk, medium yield and smal grain type, medium stalk, high yield and large grain type, medium stalk, low yield and smal grain type and high stalk, medium yield and medium grain type. Among them, performance of comprehensive trait of the second type was better than that of the other types. Thus, the F. tataricum_va-rieties （lines） that were classified into the second type could be considered as good varieties （lines） or breeding materials. The genetic differences among F. tataricum_varieties （lines） had no necessary correlations with origin and geographical distance. ln addition to complementary traits and geographical distance, genetic distances （dif-ferent populations） should be taken into consideration during parent selection in cross breeding.
基金Supported by Jiangsu High-tech projects(BG2007322) "Good feature of new varieties of Silkworm BreedingPh.D., University of Jiangsu Science and Technology Fund~~
文摘[Objective] The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines （NILs）. [Method] Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. [Result] Bombyx mori NILs carrying thermotolerance gene （new germplasm） were produced. Meanwhile,thermotolerance level of progenies of each backcross and molecular markers of NILs were determined,and then attempts were made to produce practical thermotolerance hybrids by using thermotolerance varieties whose thermotolerance gene is linked to SSR markers. [Conclusion] The study successfully construct thermotolerance NILs,monitor thermotolerance level and breeding results of progenies of each backcross,and determine molecular marker of NILs.
基金Supported by the National Standard Plan(20051079-T-469)~~
文摘[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.
文摘[Objective] This study aimed to develop a new Bombyx mori variety Shuhuang No.1 and investigate its characteristics.[Method] The new variety was developed through cross breeding and pedigree separation.Its characteristics were identified by laboratory tests and rural promotion practices in Sichuan Province.[Result]The cocoon produced by the new variety Shuhuang No.1 was golden-yellow and radiantly beautiful.Shuhuang No.1 was strong and easy to rear,uniform in development.The male and female ones could be distinguished by their larval color and markings.The cocoon yield per 10 000 the 4th instar larvae reached 20.30 kg; the length of a cocoon filament was about 1 115.0 m,and a non-broken filament was 882.95 mm long,accounting for 79.15％ of the total length.The raw silk ratio of fresh cocoon was 18.39％,and the neatness was 91.25 point.The main economic features of Shuhuang No.1 were better than those of the control.[Conclusion] The new variety had been approved by Sichuan Silkworm Evaluation Commission.It can be promoted in parent silkworm rearing areas Sichuan,Chongqing,Yangtze River basin.
基金Supported by Tobacco Project of Changsha Branch of Hunan Tobacco Corporation(CYKJ2014-01)~~
文摘[Objective] This study aimed to analyze the differences in absorption capacity of different flue-cured tobacco cultivars for chromium （Cr）, arsenic （As）, cadmium （Cd）, lead （Pb）, mercury （Hg） and copper （Cu）. [Method] With 4 flue-cured tobacco cultivars as the experimental materials, their absorption capacity for 6 kinds of heavy metals was compared by pot experiment. [Result] There were great differences in absorption capacity of different flue-cured tobacco cultivars for the six kinds of heavy metals. According to the absorption capacity of different flue-cured tobacco cultivars for the heavy metals, the six heavy metals could be classified into three categories. The first category included only Cd with average enrichment coefficient of 8.564; the second category included Hg with average enrichment coefficient of 0.753; and the third category included Cr, As, Pb and Cu with average enrichment coefficients below or equivalent to 0.114. [Conclusion] The absorption capacity of different flue-cured tobacco cultivars for heavy metals was grasped by this study, thereby providing reference data for screening of high-yielding and high-quality flue-cured tobacco cultivars.
基金Supported by the National Key Basic Research Program (973 Program) of China (No.2007CB109304)the NationalNatural Science Foundation of China (Nos.30771290 and 30671234)
文摘The variation in nitrogen （N） uptake by rice has been widely studied but differences in rice root morphology that may contribute to this variation are not completely understood. Field and greenhouse experiments were carried out to study N accumulation, root dry weights, total root lengths, root surface areas, and root bleeding rates of two rice cultivars, Elio with low N-use efficiency and Nanguang with high N-use efficiency. Low （1 mmol N L^-1） and high （5 mmol N L^-1） N applications were established in the greenhouse experiment, and the N rates were 0, 120, and 240 kg ha^-1 in the field experiments at Jiangning and Jiangpu farms, Nanjing, China. The results showed that the N accumulation, root dry weight, total root length, and root surface area increased with an increase in N application. At the heading stage, N accumulation in the shoots and roots of Nanguang was greater than that of Elio in the field experiments and that of Elio at 5 mmol N L^-1 in the greenhouse experiment. After the heading stage, N accumulation was higher for Nanguang at both 1 and 5 mmol N L^-1 in the greenhouse experiment. The total root length and root surface area were significantly different between the two cultivars. Over the range of the fertilizer application rates, the root lengths of Nanguang at Jiangning Farm were 49%-6170 greater at booting and 26%-39% greater at heading than those of Elio, and at Jiangpu Farm they were 22%-42% and 26%-38% greater, respectively. Nanguang had a greater root bleeding rate than Elio. It was concluded that the N-use efficiency of the two rice cultivars studied depended to a great extent on the root morphological parameters and root physiological characteristics at different growth stages.
基金Supported by the Program of Gansu Province Aerospace Engineering Biology Breeding Key Laboratory(139RTSE033)~~
文摘Hangjiao Hongfeng is a new hot pepper F1 hybrid cultivar which is bred with the cytoplasmic male sterile line 11066 AH as the female parent and the restoringline 11044 CH as the male parent. Its fruit is early-mid maturing and in the shape of a long finger.The immature fruit is in dark-green and mature fruit is in dark-red.The average length, width and flesh thickness are 15.7, 1.9 and 0.17 cm, respectively. The yield of dry pepper is 6 750 kg/hm^2. The average contents of Vc, soluble solid and dry matter are 1.30 mg/g, 10%, and 14% respectively. The cultivar has good resistance to pepper blight, viral disease and anthracnose. It shows strong tolerance to heat, water logging and drought. It is suitable for spring field cultivation in Gansu, Xinjiang and Inner Mongolia.
