We analyze the entanglement of two atoms in a cavity. The evolvement of entanglement of two atoms is disorderly when two atoms in the cavity are fixed at initial time. However, the evolvement of entanglement of two at...We analyze the entanglement of two atoms in a cavity. The evolvement of entanglement of two atoms is disorderly when two atoms in the cavity are fixed at initial time. However, the evolvement of entanglement of two atoms becomes well-regulated orderly under the coherent cavity field when atoms are in motion at beginning. We find that it is possible to control this periodic entanglement by properly choosing the velocity of atoms, and the initial state of the field.展开更多
Objective: To observe the activity of repeated extracts of bone matrix and the production of purified bone morphogenetic proteins (BMPs). Methods: BMPs were extracted 1- 4 times from fresh bovine cortical bone by the ...Objective: To observe the activity of repeated extracts of bone matrix and the production of purified bone morphogenetic proteins (BMPs). Methods: BMPs were extracted 1- 4 times from fresh bovine cortical bone by the modified Urist’s method, with each collected precipitate separated and lyophilized as partially purified BMPs. Another fresh bovine bone was extracted three times and the precipitates were mixed and lyophilized. Meanwhile,the alkaline phosphatase (ALP) activity was measured by an in vitro assay employing cultured C2C12 mouse myoblast cells through the osteoinductivity of bovine BMPs extracted four times at days 1, 4, 7, and 14, and the correlation between BMPs quantities and costing during extraction processes was analyzed.Results: The BMPs purified and the cost showed a positive correlation (r=(0.969)). To separate and lyophilize each collected precipitate as partially purified BMPs raised the cost, and mixed precipitates also cost much. ALP activities of the 1st and mixed extractions of BMPs were shown to be highly osteoinductive and keep a significantly high level (P<(0.05)-(0.01)) 4 days after culturing, compared with the 2nd, 3rd and 4th extractions, especially the control group. However, the more times the extraction was done, the less activity of BMPs was shown and more costing was. The x-ray and histological analysis also showed that the 1st extraction of BMPs induced more ossicles and new bone formation.Conclusions: The results indicated that BMPs enhanced the abilities of osteoinductivity in C2C12 culture in vitro. The first extraction of BMPs from bone is fitfull, the second extraction should be enough, while, the 3rd and 4th extractions are unnecessary for they cost more and waste more time, say nothing of mixed extractions.展开更多
基金The project supported by the State 973 Project under Grant No.2006CB921606by the Natural Science Foundation of Hubei Province
文摘We analyze the entanglement of two atoms in a cavity. The evolvement of entanglement of two atoms is disorderly when two atoms in the cavity are fixed at initial time. However, the evolvement of entanglement of two atoms becomes well-regulated orderly under the coherent cavity field when atoms are in motion at beginning. We find that it is possible to control this periodic entanglement by properly choosing the velocity of atoms, and the initial state of the field.
文摘Objective: To observe the activity of repeated extracts of bone matrix and the production of purified bone morphogenetic proteins (BMPs). Methods: BMPs were extracted 1- 4 times from fresh bovine cortical bone by the modified Urist’s method, with each collected precipitate separated and lyophilized as partially purified BMPs. Another fresh bovine bone was extracted three times and the precipitates were mixed and lyophilized. Meanwhile,the alkaline phosphatase (ALP) activity was measured by an in vitro assay employing cultured C2C12 mouse myoblast cells through the osteoinductivity of bovine BMPs extracted four times at days 1, 4, 7, and 14, and the correlation between BMPs quantities and costing during extraction processes was analyzed.Results: The BMPs purified and the cost showed a positive correlation (r=(0.969)). To separate and lyophilize each collected precipitate as partially purified BMPs raised the cost, and mixed precipitates also cost much. ALP activities of the 1st and mixed extractions of BMPs were shown to be highly osteoinductive and keep a significantly high level (P<(0.05)-(0.01)) 4 days after culturing, compared with the 2nd, 3rd and 4th extractions, especially the control group. However, the more times the extraction was done, the less activity of BMPs was shown and more costing was. The x-ray and histological analysis also showed that the 1st extraction of BMPs induced more ossicles and new bone formation.Conclusions: The results indicated that BMPs enhanced the abilities of osteoinductivity in C2C12 culture in vitro. The first extraction of BMPs from bone is fitfull, the second extraction should be enough, while, the 3rd and 4th extractions are unnecessary for they cost more and waste more time, say nothing of mixed extractions.
