目的:研究肝郁气滞型三阴性乳腺癌(triple negative breast cancer,TNBC)患者口腔菌群的多样性与差异性,探讨口腔菌群与肝郁气滞型TNBC的相关性。方法:根据纳排标准纳入肝郁气滞型三阴性乳腺癌患者8例作为患者组,健康志愿者10例作为正常...目的:研究肝郁气滞型三阴性乳腺癌(triple negative breast cancer,TNBC)患者口腔菌群的多样性与差异性,探讨口腔菌群与肝郁气滞型TNBC的相关性。方法:根据纳排标准纳入肝郁气滞型三阴性乳腺癌患者8例作为患者组,健康志愿者10例作为正常组,分别收集唾液标本,采用16S rDNA测序技术检测2组人群口腔菌群的丰度和分布情况,通过组间差异分析寻找组间具有显著性差异的菌属。结果:患者组与正常组的口腔菌群物种多样性显著,且2组口腔菌群分布存在显著差异(P<0.05)。其中患者组较正常组增多的物种有密螺旋体属(Treponema)、普雷沃氏菌属(Prevotella)、噬二氧化碳细胞菌属(Capnocytophaga)、坦纳菌属(Tannerella)、考拉杆菌属(Phascolarctobacterium)、金氏菌属(Kingella)、丛毛单胞菌属(Comamonas)、螺旋体科(Spirochaetaceae)等;较正常组减少的物种有韦荣氏球菌(Veillonella)、链球菌属(Streptococcus) 、己酸菌属(Caproiciproducens)、气单胞菌属(Aeromonas)等。结论:失调的口腔菌群及其细菌产物在三阴性乳腺癌的发生发展和转移中起着重要作用,同时特定菌群对中医证候的形成与变化也有一定影响。口腔菌群可作为TNBC早期诊断、预后判断以及中医证候客观化的生物标志物,对于提高TNBC的疗效、靶向干预相关不良反应、改善生活质量及预后有着重要意义。展开更多
目的:通过验证加味仙方活命饮对药物相关性颌骨坏死(medication-related osteonecrosis of the jaws, MRONJ)小鼠的治疗效果,探索加味仙方活命饮治疗MRONJ与口腔菌群变化的相关性及调节作用。方法:40只C57BL/6J小鼠随机分为对照组(NC组...目的:通过验证加味仙方活命饮对药物相关性颌骨坏死(medication-related osteonecrosis of the jaws, MRONJ)小鼠的治疗效果,探索加味仙方活命饮治疗MRONJ与口腔菌群变化的相关性及调节作用。方法:40只C57BL/6J小鼠随机分为对照组(NC组)、模型组(Model组)、特立帕肽组(PTH组)及加味仙方活命饮组(XFHMY组)。除NC组外,其余各组构建唑来膦酸(ZA)、地塞米松联合拔牙诱导的MRONJ小鼠模型,PTH组和XFHMY组分别对应给予特立帕肽(PTH)4.11μg/kg、加味仙方活命饮22.81 g/kg干预,通过Micro-CT、HE染色观察加味仙方活命饮对MRONJ小鼠的治疗效果,采集各组小鼠治疗后的口腔菌落,16S rRNA测序法分析治疗MRONJ小鼠与其口腔菌群构成、多样性、丰度的相关性及调节作用。结果:直接观察拔牙伤口愈合情况,NC组小鼠伤口愈合情况良好,而Model组小鼠伤口未愈合,PTH组和XFHMY组伤口几乎愈合、XFHMY组愈合效果更佳。对第9周取材所得上颌骨组织Micro-CT扫描显示,Model组的骨体积分数(BV/TV)和骨密度(BMD)均显著高于NC组,PTH组和XFHMY组低于Model组(P<0.05)。直接观察HE染色情况,第9周给药干预后显示NC组与Model组的活骨与死骨分布、表面纹路和面积具有明显差异;PTH、加味仙方活命饮干预后死骨陷窝面积较Model组减小,且XFHMY组的坏死骨周围出现破骨细胞,坏死骨将近脱落。口腔菌群Beta多样性分析显示,NC组与Model组组成结构具有差异(P<0.05);Model组与XFHMY组组成结构具有差异(P<0.05)。小鼠口腔菌群组成结构相关性分析显示,厚壁菌门、梭杆菌门相对丰度与MRONJ的发生成正相关(P<0.05),放线菌门、梭杆菌属相对丰度与加味仙方活命饮治疗效果成负相关(P<0.05)。结论:加味仙方活命饮治疗MRONJ促进创口愈合及骨重建的治疗效果与口腔菌群变化有关,其有效作用途径之一可能是通过调节口腔菌群,促进创面修复。展开更多
目的:本项目计划构建不同雪茄烟气浓度暴露下的小鼠实验模型,探讨雪茄烟气暴露对小鼠口腔菌群的影响。方法:将40只8周龄雄性C57BL/6小鼠随机均分到对照组、雪茄烟气低剂量组、中剂量组以及高剂量组下进行4周饲养,收集小鼠口腔唾液样本,...目的:本项目计划构建不同雪茄烟气浓度暴露下的小鼠实验模型,探讨雪茄烟气暴露对小鼠口腔菌群的影响。方法:将40只8周龄雄性C57BL/6小鼠随机均分到对照组、雪茄烟气低剂量组、中剂量组以及高剂量组下进行4周饲养,收集小鼠口腔唾液样本,利用16S核糖体RNA (16S ribosomal RNA, 16S rRNA)基因测序技术分析菌群的特征。结果:与对照组相比,不同剂量雪茄干预组小鼠口腔菌群Ace指数以及Shannon指数显著增加(p p p p Objective: This project aimed to develop a mouse model subjected to varying concentrations of cigar smoke to investigate the impact of cigar smoke exposure on the oral microbiome of mice. Method: Forty male C57BL/6 mice, aged 8 weeks, were randomly assigned into four groups: control group, low-dose cigar smoke group, medium-dose group, and high-dose group for a feeding period of four weeks. Oral saliva samples were collected from the mice, and 16S ribosomal RNA (16S rRNA) gene sequencing technology was employed to analyze oral microbiome characteristics. Results: Compared to the control group, the Ace and Shannon index of the oral microbiome in all cigar intervention groups exhibited significant increases (p p p p < 0.05). Conclusion: Cigar smoke exposure and exposure concentrations can affect the microbial composition of the oral microbiome. Additionally, different concentrations of cigars exert specific effects on oral bacteria genera in mice. This study enhances the understanding of how cigar smoke influences oral health and provides critical data for developing effective prevention strategies as well as improving processing and manufacturing methods related to cigars.展开更多
目的:探究一款后生元牙膏对健康人群口腔菌群的影响。方法:纳入30名口腔健康人群,在使用牙膏前(0 d)和使用30天后(30 d)分别采集唾液,测定PLI值和CAT值,并进行16S rDNA测序,分析两组菌群结构及多样性差异。结果:与使用前相比,使用牙膏后...目的:探究一款后生元牙膏对健康人群口腔菌群的影响。方法:纳入30名口腔健康人群,在使用牙膏前(0 d)和使用30天后(30 d)分别采集唾液,测定PLI值和CAT值,并进行16S rDNA测序,分析两组菌群结构及多样性差异。结果:与使用前相比,使用牙膏后的PLI值和CAT值均显著降低(P Capnocytophaga)和孪生球菌属(Gemella)导致。结论:该款后生元牙膏能够有效减少牙菌斑,降低龋病风险,并能够调节口腔微生态。Objective: To investigate the effect of a postbiotic toothpaste on the oral microbiota of healthy people. Methods: A total of 30 oral health individuals were enrolled, and saliva was collected before (0 day) and 30 days after 30 days (30 days) of toothpaste, and PLI and CAT values were measured, and 16S rDNA sequencing was performed to analyze the differences in microbial community structure and diversity between the two groups. Results: Compared with the before use, the PLI value and CAT value were significantly reduced after using toothpaste (P < 0.0001). The dominant bacteria in the oral microbiota were basically the same before and after the use of toothpaste, but there were differences in relative abundance. Compared with the before use, the alpha diversity index Ace and Chao were significantly decreased after using toothpaste (P < 0.0001). The results of PCA analysis showed that the differences in oral microbiota composition before and after toothpaste use were mainly caused by Capnocytophaga and Gemella. Conclusion: This postbiotic toothpaste can effectively reduce dental plaque, reduce the risk of caries, and regulate the oral microecology.展开更多
文摘目的:通过验证加味仙方活命饮对药物相关性颌骨坏死(medication-related osteonecrosis of the jaws, MRONJ)小鼠的治疗效果,探索加味仙方活命饮治疗MRONJ与口腔菌群变化的相关性及调节作用。方法:40只C57BL/6J小鼠随机分为对照组(NC组)、模型组(Model组)、特立帕肽组(PTH组)及加味仙方活命饮组(XFHMY组)。除NC组外,其余各组构建唑来膦酸(ZA)、地塞米松联合拔牙诱导的MRONJ小鼠模型,PTH组和XFHMY组分别对应给予特立帕肽(PTH)4.11μg/kg、加味仙方活命饮22.81 g/kg干预,通过Micro-CT、HE染色观察加味仙方活命饮对MRONJ小鼠的治疗效果,采集各组小鼠治疗后的口腔菌落,16S rRNA测序法分析治疗MRONJ小鼠与其口腔菌群构成、多样性、丰度的相关性及调节作用。结果:直接观察拔牙伤口愈合情况,NC组小鼠伤口愈合情况良好,而Model组小鼠伤口未愈合,PTH组和XFHMY组伤口几乎愈合、XFHMY组愈合效果更佳。对第9周取材所得上颌骨组织Micro-CT扫描显示,Model组的骨体积分数(BV/TV)和骨密度(BMD)均显著高于NC组,PTH组和XFHMY组低于Model组(P<0.05)。直接观察HE染色情况,第9周给药干预后显示NC组与Model组的活骨与死骨分布、表面纹路和面积具有明显差异;PTH、加味仙方活命饮干预后死骨陷窝面积较Model组减小,且XFHMY组的坏死骨周围出现破骨细胞,坏死骨将近脱落。口腔菌群Beta多样性分析显示,NC组与Model组组成结构具有差异(P<0.05);Model组与XFHMY组组成结构具有差异(P<0.05)。小鼠口腔菌群组成结构相关性分析显示,厚壁菌门、梭杆菌门相对丰度与MRONJ的发生成正相关(P<0.05),放线菌门、梭杆菌属相对丰度与加味仙方活命饮治疗效果成负相关(P<0.05)。结论:加味仙方活命饮治疗MRONJ促进创口愈合及骨重建的治疗效果与口腔菌群变化有关,其有效作用途径之一可能是通过调节口腔菌群,促进创面修复。
基金烟草行业雪茄发酵工艺重点实验室开放基金资助项目(20202309BC530) (Opening Fund of Cigar Fermentation Technology Key Laboratory of Tobacco Industry (20202309BC530))。
文摘目的:本项目计划构建不同雪茄烟气浓度暴露下的小鼠实验模型,探讨雪茄烟气暴露对小鼠口腔菌群的影响。方法:将40只8周龄雄性C57BL/6小鼠随机均分到对照组、雪茄烟气低剂量组、中剂量组以及高剂量组下进行4周饲养,收集小鼠口腔唾液样本,利用16S核糖体RNA (16S ribosomal RNA, 16S rRNA)基因测序技术分析菌群的特征。结果:与对照组相比,不同剂量雪茄干预组小鼠口腔菌群Ace指数以及Shannon指数显著增加(p p p p Objective: This project aimed to develop a mouse model subjected to varying concentrations of cigar smoke to investigate the impact of cigar smoke exposure on the oral microbiome of mice. Method: Forty male C57BL/6 mice, aged 8 weeks, were randomly assigned into four groups: control group, low-dose cigar smoke group, medium-dose group, and high-dose group for a feeding period of four weeks. Oral saliva samples were collected from the mice, and 16S ribosomal RNA (16S rRNA) gene sequencing technology was employed to analyze oral microbiome characteristics. Results: Compared to the control group, the Ace and Shannon index of the oral microbiome in all cigar intervention groups exhibited significant increases (p p p p < 0.05). Conclusion: Cigar smoke exposure and exposure concentrations can affect the microbial composition of the oral microbiome. Additionally, different concentrations of cigars exert specific effects on oral bacteria genera in mice. This study enhances the understanding of how cigar smoke influences oral health and provides critical data for developing effective prevention strategies as well as improving processing and manufacturing methods related to cigars.
文摘目的:探究一款后生元牙膏对健康人群口腔菌群的影响。方法:纳入30名口腔健康人群,在使用牙膏前(0 d)和使用30天后(30 d)分别采集唾液,测定PLI值和CAT值,并进行16S rDNA测序,分析两组菌群结构及多样性差异。结果:与使用前相比,使用牙膏后的PLI值和CAT值均显著降低(P Capnocytophaga)和孪生球菌属(Gemella)导致。结论:该款后生元牙膏能够有效减少牙菌斑,降低龋病风险,并能够调节口腔微生态。Objective: To investigate the effect of a postbiotic toothpaste on the oral microbiota of healthy people. Methods: A total of 30 oral health individuals were enrolled, and saliva was collected before (0 day) and 30 days after 30 days (30 days) of toothpaste, and PLI and CAT values were measured, and 16S rDNA sequencing was performed to analyze the differences in microbial community structure and diversity between the two groups. Results: Compared with the before use, the PLI value and CAT value were significantly reduced after using toothpaste (P < 0.0001). The dominant bacteria in the oral microbiota were basically the same before and after the use of toothpaste, but there were differences in relative abundance. Compared with the before use, the alpha diversity index Ace and Chao were significantly decreased after using toothpaste (P < 0.0001). The results of PCA analysis showed that the differences in oral microbiota composition before and after toothpaste use were mainly caused by Capnocytophaga and Gemella. Conclusion: This postbiotic toothpaste can effectively reduce dental plaque, reduce the risk of caries, and regulate the oral microecology.
