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基于双相动力学分析靶向药物联合应用对肝癌细胞SNU-387增殖的影响及其作用机制
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作者 刘畅 裴晋红 +1 位作者 穆秀丽 于保锋 《中国生物制品学杂志》 CAS CSCD 北大核心 2021年第10期1184-1190,共7页
目的基于双相动力学分析靶向药物联合应用对肝癌细胞SNU-387增殖的影响,并探讨其作用机制。方法 MTT法检测肝癌细胞SNU-387对11种酪氨酸激酶抑制剂(AZD6244、AZD6482、BGJ-398、BMS-754807、bosutinib、crizotinib、dasatinib、gefitini... 目的基于双相动力学分析靶向药物联合应用对肝癌细胞SNU-387增殖的影响,并探讨其作用机制。方法 MTT法检测肝癌细胞SNU-387对11种酪氨酸激酶抑制剂(AZD6244、AZD6482、BGJ-398、BMS-754807、bosutinib、crizotinib、dasatinib、gefitinib、imatinib、sunitinib、HG6-64-1)的敏感性;分别采用单靶点动力学公式和双相动力学公式分析肝癌细胞SNU-387与BMS-754807、dasatinib和HG6-64-1的相互作用,计算拟合曲线的残差平方和(the sums of squared residues,SSR);Western blot法检测BMS-754807、dasatinib和HG6-64-1对SNU-387细胞关键信号通路的影响;MTT法检测BMS-754807、dasatinib和HG6-64-1联合应用对SNU-387细胞增殖的影响,计算减量指数(dose reduction index,DRI)。结果 SNU-387细胞对BMS-754807、HG6-64-1及1 μmol/L的dasatinib比较敏感。与单靶点动力学公式比较,双相动力学公式可更好地分析肝癌细胞SNU-387与酪氨酸激酶抑制剂的相互作用。BMS-754807、dasatinib和HG6-64-1联合应用时F1相靶点不重合,对SNU-387细胞增殖具有显著协同抑制作用。结论 BMS-754807、dasatinib和HG6-64-1通过同时抑制不同靶点协同抑制肝癌细胞SNU-387的增殖,三者联合应用有望成为一种治疗肝癌的潜在药物组合。 展开更多
关键词 双相动力学分析 肝癌细胞 蛋白激酶抑制剂 联合靶向治疗 减量指数
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右美托咪定双相血流动力学变化在老年患者全麻诱导中的应用 被引量:3
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作者 邓书 郭玲 柳兆芳 《蚌埠医学院学报》 CAS 2016年第9期1185-1187,共3页
目的:探讨右美托咪定的双相血流动力学变化在老年患者全麻诱导中的应用。方法:选择全麻老年患者50例,ASAⅠ~Ⅱ级,随机分为右美托咪定组(D组)和0.9%氯化钠注射液组(C组),每组25例。D组麻醉诱导前给予右美托咪定负荷量0.5μg/kg稀释至20 ... 目的:探讨右美托咪定的双相血流动力学变化在老年患者全麻诱导中的应用。方法:选择全麻老年患者50例,ASAⅠ~Ⅱ级,随机分为右美托咪定组(D组)和0.9%氯化钠注射液组(C组),每组25例。D组麻醉诱导前给予右美托咪定负荷量0.5μg/kg稀释至20 m L泵注,10 min输注完毕,再以0.3μg·kg^(-1)·h^(-1)泵注至插管后5 min;C组静脉泵注等量0.9%氯化钠注射液。分别记录入室后(T0),右美托咪定负荷量结束后(T_1),气管插管前(T_2)及插管后1 min(T_3)、3 min(T_4)、5 min(T_5)时的平均动脉压(MAP)、心率(HR)。结果:2组患者MAP在T_2~T_5时点均较T0下降(P<0.05~P<0.01);D组MAP在T_1、T_2、T-4、T_5时点与T_3差异均无统计学意义(P>0.05),C组T_1和T_2时点MAP与T3差异均有统计学意义(P<0.01);D组T_2时点MAP高于C组(P<0.05)。D组患者HR在T_1、T_2、T_4和T_5时点明显低于T0(P<0.01);T_2、T_4和T_5的HR均显著低于T_3(P<0.01);C组患者T_4和T_5时点HR均低于T0(P<0.05);D组T_1、T_2和T_5的HR均明显低于C组(P<0.01)。结论:全麻诱导过程中给予右美托咪定可以升高动脉血压,一定程度地抑制了全麻药物引起的血压下降,同时降低HR,减弱气管插管反应,稳定心血管功能。 展开更多
关键词 麻醉 右美托咪定 双相血流动力学变化 老年
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杨木水热预处理过程中水解反应动力学研究 被引量:3
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作者 吴彦 田中建 +3 位作者 陈嘉川 张凤山 房桂干 吉兴香 《林产化学与工业》 CAS CSCD 北大核心 2022年第1期21-28,共8页
探讨了预处理温度和预处理时间对杨木水热预处理液中各组分含量的影响,同时采用改良的Saeman双相动力学模型建立了预处理液中木糖和葡萄糖的反应动力学方程,通过非线性拟合确定了不同条件下木糖和葡萄糖生成及分解的反应速率常数,由阿... 探讨了预处理温度和预处理时间对杨木水热预处理液中各组分含量的影响,同时采用改良的Saeman双相动力学模型建立了预处理液中木糖和葡萄糖的反应动力学方程,通过非线性拟合确定了不同条件下木糖和葡萄糖生成及分解的反应速率常数,由阿伦尼乌斯公式线性拟合得出木质纤维原料水解反应活化能。杨木预处理过程中木聚糖易降解部分活化能(E_(f))为78.35 kJ/mol,难降解部分活化能(E_(s))为88.97 kJ/mol,木糖分解的活化能为80.17 kJ/mol;葡聚糖降解活化能(48.06 kJ/mol)低于葡萄糖分解活化能(90.18 kJ/mol)。研究结果表明:葡萄糖比木糖更加稳定,水热预处理温和的处理条件可实现溶解半纤维素的同时减少纤维素和单糖的降解。 展开更多
关键词 杨木 水热预处理 双相动力学模型
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靶向药物联合应用对肝癌细胞SK-Hep-1增殖的影响及其机制 被引量:1
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作者 朱晓霞 贾瑜琦 +4 位作者 刘畅 弓韬 李高鹏 张宏伟 于保锋 《肿瘤防治研究》 CAS CSCD 2022年第11期1126-1133,共8页
目的探讨靶向药物联合应用对多驱动基因调控增殖的肝癌细胞SK-Hep-1的影响及作用机制。方法利用对肝癌细胞SK-Hep-1敏感的靶向药物(HG6-64-1、dasatinib、crizotinib、sunitinib)绘制单靶点动力学分析曲线和双相分析曲线,对SK-Hep-1细... 目的探讨靶向药物联合应用对多驱动基因调控增殖的肝癌细胞SK-Hep-1的影响及作用机制。方法利用对肝癌细胞SK-Hep-1敏感的靶向药物(HG6-64-1、dasatinib、crizotinib、sunitinib)绘制单靶点动力学分析曲线和双相分析曲线,对SK-Hep-1细胞进行动力学分析;Western blot法检测这些靶向药物对SK-Hep-1细胞关键信号通路的影响;MTT法检测这些药物单用和联合应用对SK-Hep-1细胞增殖的影响。结果与单靶点动力学分析曲线比较,双相分析曲线可更好拟合靶向药物对肝癌细胞SK-Hep-1的影响,并且预测出HG6-64-1、dasatinib和MK-2206三药联合应用能有效抑制SK-Hep-1细胞增殖。结论双相动力学分析可以更好地定量描述多驱动增殖的肝癌细胞SK-Hep-1对靶向治疗的反应,联合使用HG6-64-1、dasatinib和MK-2206是治疗肝癌潜在的药物组合。 展开更多
关键词 肝细胞癌 双相动力学分析 多驱动增殖 靶向药物 作用机制 药物联合
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米托蒽醌白蛋白微球和糖蛋白微球的体外释药规律 被引量:1
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作者 张奇志 张志荣 《华西药学杂志》 CAS CSCD 2001年第4期263-264,共2页
目的 :考察米托蒽醌白蛋白微球和米托蒽醌联糖白蛋白微球的体外释药规律。方法 :采用动态透析法释药 ,分光光度法测定米托蒽醌含量。结果 :二者在体外释药符合双指数双相动力学规律 ,但后者达释药平衡快(约 6小时 ) ,累积释放量几乎比... 目的 :考察米托蒽醌白蛋白微球和米托蒽醌联糖白蛋白微球的体外释药规律。方法 :采用动态透析法释药 ,分光光度法测定米托蒽醌含量。结果 :二者在体外释药符合双指数双相动力学规律 ,但后者达释药平衡快(约 6小时 ) ,累积释放量几乎比前者小一倍。结论 :两种微球的累积释药分数经单因数方差分析 。 展开更多
关键词 米托蒽醌白蛋白微球 米托蒽醌联糖白蛋白微球 体外释药 双相动力学 抗癌药
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Simulation and Analysis on the Two-Phase Flow Fields in a Rotating-Stream-Tray Absorber by Using Computational Fluid Dynamics 被引量:8
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作者 邵雄飞 吴忠标 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2004年第2期169-173,共5页
The flow field of gas and liquid in a φ150mm rotating-stream-tray (RST) scrubber is simulated by using computational fluid dynamic (CFD) method. The sismulation is based on the two-equation RNG κ-ε turbulence model... The flow field of gas and liquid in a φ150mm rotating-stream-tray (RST) scrubber is simulated by using computational fluid dynamic (CFD) method. The sismulation is based on the two-equation RNG κ-ε turbulence model, Eulerian multiphase model, and a real-shape 3D model with a huge number of meshes. The simulation results include detailed information about velocity, pressure, volume fraction and so on. Some features of the flow field are obtained: liquid is atomized in a thin annular zone; a high velocity air zone prevents water drops at the bottom from flying towards the wall; the pressure varies sharply at the end of blades and so on. The results will be helpful for structure optimization and engineering design. 展开更多
关键词 rotating-stream-tray two-phase flow field SIMULATION computational fluid dynamics
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Austenite formation during intercritical annealing in C-Mn cold-rolled dual phase steel 被引量:1
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作者 李声慈 康永林 +1 位作者 朱国明 邝霜 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第4期1203-1211,共9页
Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser sc... Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser scanning microscope, and the austenite isothermal and non-isothermal transformation kinetics were studied by dilatometry. The results indicate that banded structure is produced for the reason of composition segregation and the competition between recrystallization and phase transformation. Austenite prefers to nucleate not only at ferrite/ferrite grain boundaries, but also inside the grains of ferrite.Furthermore, the austenitizing process is accomplished mainly via migration of the existing austenite/ferrite interface rather than nucleation of new grains. The incubation process can be divided into two stages which are controlled by carbon and manganese diffusion, respectively. During the incubation process, the nucleation rate of austenite decreases, and austenite growth changes from two-dimensional to one-dimensional. The partitioning coefficient, defined as the ratio of manganese content in the austenite to that in the adjacent ferrite, increases with increasing soaking time. 展开更多
关键词 dual phase steel confocal laser scanning microscope dilatometry austenitizing in situ observation
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Kinetics of the Double Carbonylation of Benzylchloride
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作者 欧阳平凯 范伟平 张湜 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2000年第4期379-384,共6页
It is a multi-phase-catalyzed reaction to produce calcium phenylpyruvate by double carbonylation of benzylchloride. Based on the analysis of the reaction mechanism, a kinetic model of the carbonylation reaction was o... It is a multi-phase-catalyzed reaction to produce calcium phenylpyruvate by double carbonylation of benzylchloride. Based on the analysis of the reaction mechanism, a kinetic model of the carbonylation reaction was obtained. The model was verified through experiments in which the diffusion effect was neglected with the appropriate operation manner. But it is inevitable that the carbonylation process is controlled by diffusion as the autoclave scaling up. 展开更多
关键词 benzylchloride CARBONYLATION KINETICS
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Corona-Support for the Entirety of High Energetic Disk
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作者 Krasimira Dimitrova Yankova 《Journal of Earth Science and Engineering》 2012年第7期395-400,共6页
Based on results obtained from the study for MHD (magneto-hydrodynamics) of advective accretion disk, which are applied to real source showing typical values for CBS (close binary star-system), it will investigate... Based on results obtained from the study for MHD (magneto-hydrodynamics) of advective accretion disk, which are applied to real source showing typical values for CBS (close binary star-system), it will investigate on self-structuring in the disk under the impact of the distribution of leading parameters (density, velocity ...). The paper is considering the problem of development of the corona and will analyze the process of interaction of the plasma with the magnetic field in connection to support for the instabilities. 展开更多
关键词 Accretion disk advection MHD (magneto-hydrodynamics) instabilities.
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大豆油-甲酸-H_2O_2环氧化过程的建模 被引量:3
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作者 冯树波 刘志雪 +2 位作者 陈佳 宋亚玲 郑二丽 《计算机与应用化学》 CAS CSCD 北大核心 2012年第9期1156-1158,共3页
基于碘值、环氧值和过氧化氢浓度实验数据建立了大豆油环氧化过程的双相动力学模型。反应网络包括甲酸与过氧化氢生成过氧甲酸、过氧化氢直接分解和环氧化物的水解3个副反应以及过氧甲酸与双键的环氧化主反应。借助各物种在油水两相的... 基于碘值、环氧值和过氧化氢浓度实验数据建立了大豆油环氧化过程的双相动力学模型。反应网络包括甲酸与过氧化氢生成过氧甲酸、过氧化氢直接分解和环氧化物的水解3个副反应以及过氧甲酸与双键的环氧化主反应。借助各物种在油水两相的分配系数确定了相关组分在两相中的浓度。建模过程中,假设过氧甲酸的生成为速率控制步骤,确定了每个反应的动力学参数。模拟预测结果与实验数据吻合较好,说明本文建立的数学模型可用于大豆油环氧化结果的预测,可为设计工业反应器提供指导。 展开更多
关键词 大豆油环氧化 双相动力学模型 建模 分配系数
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Modeling and Measurements of Heat Transfer Phenomena in Two-Phase PbSn Alloy Solidification in an External Magnetic Field
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作者 P.A.Nikrityuk K.Eckert +2 位作者 R.Grundmann B. Willers S. Eckert 《Journal of Thermal Science》 SCIE EI CAS CSCD 2003年第4期357-362,共6页
The main aim of this work is to study numerically the influence of an external magnetic field on the solidification processes of two-component materials. Based on the continuum model of two-phase flow a mathematical m... The main aim of this work is to study numerically the influence of an external magnetic field on the solidification processes of two-component materials. Based on the continuum model of two-phase flow a mathematical model for the directional solidification of a binary alloy in a magnetic field is presented. The model includes mass, momentum, energy and species mass conservation equations written in compressible form and additional relationships describing the temperature-solute coupling. The geometry under study is a cylindrical mold with adiabatic walls and cooled bottom. The macroscale transport in the solidification of alloys is governed by the progress of the two-phase mushy zone, which is treated by means of a porous medium approach. The volume fraction of liquid and solid phases, respectively, is calculated from a 2D approximation of the phase diagram. The results of calculation are compared with experimental data. 展开更多
关键词 SOLIDIFICATION MEASUREMENTS mushy zone Lorentz force rotating magnetic field.
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Axiomatization of Special Relativity in First Order Logic
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作者 罗懿晨 陈磊 +2 位作者 何宛亭 马永革 张昕禹 《Communications in Theoretical Physics》 SCIE CAS CSCD 2016年第7期19-28,共10页
The axiomatization of physical theories is a fundamental issue of science. The first-order axiomatic system SpecR el for special relativity proposed recently by Andr′eka et al. is not enough to explain all the main r... The axiomatization of physical theories is a fundamental issue of science. The first-order axiomatic system SpecR el for special relativity proposed recently by Andr′eka et al. is not enough to explain all the main results in the theory, including the twin paradox and energy-mass relation. In this paper, from a four-dimensional spacetime perspective, we introduce the concepts of world-line, proper time and four-momentum to our axiomatic system SpecR el^+. Then we introduce an axiom of mass(Ax Mass) and take four-momentum conservation as an axiom(Ax CFM)in SpecR el^+. It turns out that the twin paradox and energy-mass relation can be derived from SpecR el+logically. Hence,as an extension of SpecR el, SpecR el^+is a suitable first-order axiomatic system to describe the kinematics and dynamics of special relativity. 展开更多
关键词 special relativity first-order logic axiomatization
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