Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded conc...Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPl staining. Band expressions of Fas, Fas ligand, poly （ADP-ribose） polyrnerase （PARP） and D-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed. Results Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner. Conclusion Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.展开更多
When a Nd：YAG laser, the wavelength of which is permeable for diamond, is focused on the surface of a diamond sample, a layer of surface material is ablated. Therefore, diamond can be cut by repetitive irradiation. I...When a Nd：YAG laser, the wavelength of which is permeable for diamond, is focused on the surface of a diamond sample, a layer of surface material is ablated. Therefore, diamond can be cut by repetitive irradiation. In this study, the processing mechanism of ablation for a single crystal diamond was examined using a heat conduction analysis, considering laser absorption at the surface or the temperature dependence of absorption coefficient. When the laser beam is absorbed at the surface layer, the surface layer is ablated during an early period in the laser pulse. Once the absorption surface layer is ablated, the laser beam penetrates the base material and ablation stops. On the other hand, if the authors assume that single crystal diamond has the temperature dependence of absorption coefficient which is about equal to that of CVD （chemical vapor deposition） diamond, the temperature rise is not enough to ablate the material. However, it became clear that the diamond is ablated deeply when the authors consider both absorption at the surface layer and the temperature dependence of the absorption coefficient. It can be considered that the surface is transformed to graphite and becomes the absorption layer during the repetitive irradiation. It is estimated that the phase change to graphite is very small and its volume fraction is a few percent at most.展开更多
Objective To investigate the effects ofamiodarone （AMD） on simvastatin （SV） in human liver microsomes and the possible underlying mechanisms. Methods Time-, NADPH- and concentration-dependent inhibitions were test...Objective To investigate the effects ofamiodarone （AMD） on simvastatin （SV） in human liver microsomes and the possible underlying mechanisms. Methods Time-, NADPH- and concentration-dependent inhibitions were tested in HLM. The logarithm of relative inhibition values was plotted versus preincubation time （0, 5, 10, 15, 20min） for a series concentration of AMD used （0, 2, 5, 25, 50 umol/L）, and the slopes determined by linear regression. These slope values represente the observed inactivation rate constants （kobs ）. A double-reciprocal plot was then constructed using the reciprocal of the kobs （y-axis） and the reciprocal of the associated inhibitor concentration （x-axis） to estimate the values of kinact and K1, which were two principal kinetic constants that were specific for mechanism-based inhibition （MBI）.drug-drug interactions （DDI） potential was predicted based on in vitro data and by using the in vitro-in vivo extrapolation. Results The time-, concentration- and NADPH-dependent characteristics confirmed that when SV was the substrate of CYP3A4, the inhibition of AMD to CYP3A4 is MBI. KI and kinact value were calculated to be 5.1umol/L and 0.018minL The CLant of SV was reduced 2.96-5.63 fold when it was administrated with AMD. Conclusion Based on the results, AMD would inhibit SV metabolism via the mechanism-based manner, which would lead to DDI when they are taken together. Careful clinical observation is recommended when AMD and SV have to be simultaneously prescribed.展开更多
The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： H...The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： Human ovarian cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide （PI） staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results： LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group （P 〈 0.05）, and the ICso was 4.28 pg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable dif- ference compared with NS group （P 〈 0.05）. Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion： LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro.展开更多
The actual state of affairs of Chinese social organizations and the latest theoretical findings on the subject indicate that independence and autonomy are concepts that are interrelated but not fundamentally interchan...The actual state of affairs of Chinese social organizations and the latest theoretical findings on the subject indicate that independence and autonomy are concepts that are interrelated but not fundamentally interchangeable; their actual organizational relationship is complex and varied. Exploration of the relationship between the two concepts is a necessary step in the construction and improvement of a rule-based interpretation of the characteristics of Chinese social organizations. Combining resource dependence theory with the institutional logics perspective enables us to analyze the multiple mechanisms influencing the relationship between independence and autonomy at the organizational level and to test for these mechanisms using a mixed methods research design. Our empirical findings indicate that social organizations gain greater autonomy when they have greater independence from government resources and when they strongly identify, structurally and behaviorally, with classical third sector theory. Organizations＇ behavioral identification can regulate the relationship between independence and autonomy. Further case studies support the value of the above findings. This shows that other mechanisms can often replace, offset or constrain the effects of resource dependence. In the real world, no single fixed theory can cover the independence-autonomy relationship.展开更多
A scorpion-shaped di-NBD(4-substituted-7-nitrobenzoxadiazole) derivative of cholesterol(Chol-2NBD) was designed and synthesized. The gelation behaviors of the compound in a series of single and mixed liquids were test...A scorpion-shaped di-NBD(4-substituted-7-nitrobenzoxadiazole) derivative of cholesterol(Chol-2NBD) was designed and synthesized. The gelation behaviors of the compound in a series of single and mixed liquids were tested. It was shown that the compound is an effective gelator for mixture liquids of THF and benzene at room temperature. Furthermore, FT-IR and temperature-/concentration-dependent 1H NMR spectroscopy studies revealed that hydrogen bonding and π-π stacking among the molecules of Chol-2NBD are two main driving forces for the physical gelation of the mixture liquids. Interestingly, as observed in the gelation test and confirmed by rheological studies, the Chol-2NBD-THF/benzene gel systems, at least the one with 2:8 of the volume ratio of THF to benzene, are mechanically stable, but very sensitive to the stimulus of shear stress, which means that the gel changes into a liquid upon shaking. More interestingly, the liquid returns to gel instantly once the shear stress is removed. This phase transition process could be repeated for many times at room temperature. In addition, primary tests demonstrated that the fluorescence emission of Chol-2NBD is significantly quenched by the presence of water, ammonia water, or ammonia gas, but the emission recovers after evaporation of them. Further detailed investigation is under progress.展开更多
United Nations Political Declaration 2011 on HIV and AIDS calls to reduce the sexual transmission and the transmission of HIV among people, who inject drugs by 50% by 2015, through different control strategies and pre...United Nations Political Declaration 2011 on HIV and AIDS calls to reduce the sexual transmission and the transmission of HIV among people, who inject drugs by 50% by 2015, through different control strategies and precautionary measures. In this paper, we propose and study a simple SI type model that considers the effect of various precaution- ary measures to control HIV epidemic. We show, unlike conventional epidemic models, that the basic reproduction number which essentially considered as the disease eradica- tion condition is no longer sufficient to eliminate HIV infection. In particular, we show that even when the basic reproduction number is made less than unity, the disease may persist if the initial outbreak is not low. Eradication of disease is however guaranteed if the ensemble control measure exceeds some upper critical value. It is also shown that an epidemic model with mass action incidence may exhibit backward bifurcation and bistability if density-dependent demography is considered. Our theoretical study thus indicates that extra attention should be given in controlling HIV epidemic to achieve the desired result.展开更多
基金Supported by the Beijing Natural Science Foundation(7102128)
文摘Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPl staining. Band expressions of Fas, Fas ligand, poly （ADP-ribose） polyrnerase （PARP） and D-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed. Results Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner. Conclusion Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.
文摘When a Nd：YAG laser, the wavelength of which is permeable for diamond, is focused on the surface of a diamond sample, a layer of surface material is ablated. Therefore, diamond can be cut by repetitive irradiation. In this study, the processing mechanism of ablation for a single crystal diamond was examined using a heat conduction analysis, considering laser absorption at the surface or the temperature dependence of absorption coefficient. When the laser beam is absorbed at the surface layer, the surface layer is ablated during an early period in the laser pulse. Once the absorption surface layer is ablated, the laser beam penetrates the base material and ablation stops. On the other hand, if the authors assume that single crystal diamond has the temperature dependence of absorption coefficient which is about equal to that of CVD （chemical vapor deposition） diamond, the temperature rise is not enough to ablate the material. However, it became clear that the diamond is ablated deeply when the authors consider both absorption at the surface layer and the temperature dependence of the absorption coefficient. It can be considered that the surface is transformed to graphite and becomes the absorption layer during the repetitive irradiation. It is estimated that the phase change to graphite is very small and its volume fraction is a few percent at most.
文摘Objective To investigate the effects ofamiodarone （AMD） on simvastatin （SV） in human liver microsomes and the possible underlying mechanisms. Methods Time-, NADPH- and concentration-dependent inhibitions were tested in HLM. The logarithm of relative inhibition values was plotted versus preincubation time （0, 5, 10, 15, 20min） for a series concentration of AMD used （0, 2, 5, 25, 50 umol/L）, and the slopes determined by linear regression. These slope values represente the observed inactivation rate constants （kobs ）. A double-reciprocal plot was then constructed using the reciprocal of the kobs （y-axis） and the reciprocal of the associated inhibitor concentration （x-axis） to estimate the values of kinact and K1, which were two principal kinetic constants that were specific for mechanism-based inhibition （MBI）.drug-drug interactions （DDI） potential was predicted based on in vitro data and by using the in vitro-in vivo extrapolation. Results The time-, concentration- and NADPH-dependent characteristics confirmed that when SV was the substrate of CYP3A4, the inhibition of AMD to CYP3A4 is MBI. KI and kinact value were calculated to be 5.1umol/L and 0.018minL The CLant of SV was reduced 2.96-5.63 fold when it was administrated with AMD. Conclusion Based on the results, AMD would inhibit SV metabolism via the mechanism-based manner, which would lead to DDI when they are taken together. Careful clinical observation is recommended when AMD and SV have to be simultaneously prescribed.
文摘The purpose of this study was to investigate the effect of Laggera alata flavonen （LAF） on the inhibit- ing effect of human ovarian cancer HO-8910 cells proliferation and its possible mechanism in vitro. Methods： Human ovarian cancer HO-8910 cells were cultured in vitro. Inhibitory effect of LAF on the viability of HO-8910 cells was evaluated by the MTT assay. Apoptotic effect of different concentrations of LAF on HO-8910 cells was assessed by AO/EB staining and FCM with propidium iodide （PI） staining. Expression of proteins related to apoptosis was analyzed by Western blot. Results： LAF significantly inhibited the viability of HO-8910 cells proliferation in a dose-dependent and time-dependent manner, there were statistical significance compared with NS group （P 〈 0.05）, and the ICso was 4.28 pg/mL for 48 h. The cells treated with LAF showed typical morphological change and apoptotic rate increased by FCM in a dose-dependent, and there was notable dif- ference compared with NS group （P 〈 0.05）. Western blot showed that expression of Fas, caspase-8, tBid and Cyto-c proteins were up-regulated after treatment with LAF for 48 h in a concentration dependent. Conclusion： LAF could inhibit HO-8910 cells proliferation and induce apoptosis, which may be through the pathway of death receptor in vitro.
文摘The actual state of affairs of Chinese social organizations and the latest theoretical findings on the subject indicate that independence and autonomy are concepts that are interrelated but not fundamentally interchangeable; their actual organizational relationship is complex and varied. Exploration of the relationship between the two concepts is a necessary step in the construction and improvement of a rule-based interpretation of the characteristics of Chinese social organizations. Combining resource dependence theory with the institutional logics perspective enables us to analyze the multiple mechanisms influencing the relationship between independence and autonomy at the organizational level and to test for these mechanisms using a mixed methods research design. Our empirical findings indicate that social organizations gain greater autonomy when they have greater independence from government resources and when they strongly identify, structurally and behaviorally, with classical third sector theory. Organizations＇ behavioral identification can regulate the relationship between independence and autonomy. Further case studies support the value of the above findings. This shows that other mechanisms can often replace, offset or constrain the effects of resource dependence. In the real world, no single fixed theory can cover the independence-autonomy relationship.
基金supported by the National Natural Science Foundation of China(91027017 and 21273141)the Ministry of Education of China for its"Program for Changjiang Scholars and Innovative Research Team in University"of China(IRT1070)
文摘A scorpion-shaped di-NBD(4-substituted-7-nitrobenzoxadiazole) derivative of cholesterol(Chol-2NBD) was designed and synthesized. The gelation behaviors of the compound in a series of single and mixed liquids were tested. It was shown that the compound is an effective gelator for mixture liquids of THF and benzene at room temperature. Furthermore, FT-IR and temperature-/concentration-dependent 1H NMR spectroscopy studies revealed that hydrogen bonding and π-π stacking among the molecules of Chol-2NBD are two main driving forces for the physical gelation of the mixture liquids. Interestingly, as observed in the gelation test and confirmed by rheological studies, the Chol-2NBD-THF/benzene gel systems, at least the one with 2:8 of the volume ratio of THF to benzene, are mechanically stable, but very sensitive to the stimulus of shear stress, which means that the gel changes into a liquid upon shaking. More interestingly, the liquid returns to gel instantly once the shear stress is removed. This phase transition process could be repeated for many times at room temperature. In addition, primary tests demonstrated that the fluorescence emission of Chol-2NBD is significantly quenched by the presence of water, ammonia water, or ammonia gas, but the emission recovers after evaporation of them. Further detailed investigation is under progress.
文摘United Nations Political Declaration 2011 on HIV and AIDS calls to reduce the sexual transmission and the transmission of HIV among people, who inject drugs by 50% by 2015, through different control strategies and precautionary measures. In this paper, we propose and study a simple SI type model that considers the effect of various precaution- ary measures to control HIV epidemic. We show, unlike conventional epidemic models, that the basic reproduction number which essentially considered as the disease eradica- tion condition is no longer sufficient to eliminate HIV infection. In particular, we show that even when the basic reproduction number is made less than unity, the disease may persist if the initial outbreak is not low. Eradication of disease is however guaranteed if the ensemble control measure exceeds some upper critical value. It is also shown that an epidemic model with mass action incidence may exhibit backward bifurcation and bistability if density-dependent demography is considered. Our theoretical study thus indicates that extra attention should be given in controlling HIV epidemic to achieve the desired result.
