通过使用美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库检索出斑马鱼Smyd1a蛋白的氨基酸序列信息,对其进行生理生化分析,并通过NBCI寻找同源性高的不同种属的序列构建系统发育树。结果显示:Smy...通过使用美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库检索出斑马鱼Smyd1a蛋白的氨基酸序列信息,对其进行生理生化分析,并通过NBCI寻找同源性高的不同种属的序列构建系统发育树。结果显示:Smyd1a蛋白的氨基酸数量是489个,分子量是56376.54 Da,理论等电点是6.27;该蛋白中,谷氨酸(Glu)含量最高,为8.4%;其次是亮氨酸(Leu)和丙氨酸(Ala),分别为8.0%和7.0%。Smyd1a是一个亲水蛋白质,但不是一个分泌蛋白,不存在跨膜螺旋区域,存在蛋白质的无规则卷曲等构象,Smyd1a属于SET家族。展开更多
Microspheres Ⅰ,Ⅱ and Ⅲ were produced by emulsion technique.Microsphere I was solidified by glutaraldehyde crosslinking,microsphere Ⅱ was solidified by glutaraldehyde crosslinking and further treated with glycine s...Microspheres Ⅰ,Ⅱ and Ⅲ were produced by emulsion technique.Microsphere I was solidified by glutaraldehyde crosslinking,microsphere Ⅱ was solidified by glutaraldehyde crosslinking and further treated with glycine solution and microsphere Illwas solidified by heating denaturation only.The results showed that the microsphere diameter produced by cross[inking was bigger than that prepared by heating.The microsphere Ⅱ had higher hydrophilicity than Microsphere I had.The methotrexate (MTX) contents in microspheres Ⅰ and Ⅱ were 2.73±0.053%,2.87±0.119% respectively. microsphere Ⅲ was only blank microspheres with MTX adsorbed on their surfaces.In vitro release studies,microspheres I and I have maintained sustained release of MTX till the next day,it was found that the drug releases from microspheres Ⅰ and Ⅱ were governed by Higuchi diffusion law.展开更多
文摘通过使用美国国立生物技术信息中心(National Center for Biotechnology Information,NCBI)数据库检索出斑马鱼Smyd1a蛋白的氨基酸序列信息,对其进行生理生化分析,并通过NBCI寻找同源性高的不同种属的序列构建系统发育树。结果显示:Smyd1a蛋白的氨基酸数量是489个,分子量是56376.54 Da,理论等电点是6.27;该蛋白中,谷氨酸(Glu)含量最高,为8.4%;其次是亮氨酸(Leu)和丙氨酸(Ala),分别为8.0%和7.0%。Smyd1a是一个亲水蛋白质,但不是一个分泌蛋白,不存在跨膜螺旋区域,存在蛋白质的无规则卷曲等构象,Smyd1a属于SET家族。
文摘Microspheres Ⅰ,Ⅱ and Ⅲ were produced by emulsion technique.Microsphere I was solidified by glutaraldehyde crosslinking,microsphere Ⅱ was solidified by glutaraldehyde crosslinking and further treated with glycine solution and microsphere Illwas solidified by heating denaturation only.The results showed that the microsphere diameter produced by cross[inking was bigger than that prepared by heating.The microsphere Ⅱ had higher hydrophilicity than Microsphere I had.The methotrexate (MTX) contents in microspheres Ⅰ and Ⅱ were 2.73±0.053%,2.87±0.119% respectively. microsphere Ⅲ was only blank microspheres with MTX adsorbed on their surfaces.In vitro release studies,microspheres I and I have maintained sustained release of MTX till the next day,it was found that the drug releases from microspheres Ⅰ and Ⅱ were governed by Higuchi diffusion law.
