Methylchloroisothiazolinone and methylisothiazolinone (MCI/ MI) contact allergy affects 1-3%of patch-tested patients in European centres. The aim of the present study was to evaluate the importance of the factors -tim...Methylchloroisothiazolinone and methylisothiazolinone (MCI/ MI) contact allergy affects 1-3%of patch-tested patients in European centres. The aim of the present study was to evaluate the importance of the factors -time and concentration (dose/per unit area) -in the elicitation capacity by means of a repeated open application test (ROAT) experimental design. The study was designed as a double-blind, placebo-controlled, dose-response ROAT preceded by a diagnostic patch testing. 25 subjects with confirmed MCI/MI allergy and 10 healthy, non-MCI/MI allergic control subjects were exposed to 0.025 μg/cm2 (2 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. After a wash-out period of at least 4 weeks, the subjects were exposed to 0.094 μg/cm2 (7.5 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. The study showed the importance of both time and exposure in the elicitation process. It demonstrated that the elicitation threshold for MCI/MI is expected to be in the proximity of 0.025 μg/cm2 although it was not possible to establish a definitive elicitation threshold for MCI/MI in this study.展开更多
PURPOSE. To evaluate the reliability of self-reported dry eye disease status and patient-related predictors of misclassification in contact lens wearers. ME THODS. Patients completed the Contact Lens Dry Eye Questionn...PURPOSE. To evaluate the reliability of self-reported dry eye disease status and patient-related predictors of misclassification in contact lens wearers. ME THODS. Patients completed the Contact Lens Dry Eye Questionnaire (CLDEQ) short form on two occ asions. Test-retest reliability of the CLDEQ composite score was determined usi ng the 95%limits of agreement (LoA) and an intraclass correlation coefficient ( ICC). The κstatistic was used to determine reliability of disease-classificati on-based CLDEQ composite score cutoff points. Predictors of misclassification w ere determined by multivariate logistic regression. RESULTS. The sample included 274 patients. The range of CLDEQ composite scores from both visits was -1.83 t o 4.50 and the mean difference between administrations was -0.05±0.75 (P=0.30) . The 95%LoA of the CLDEQ composite score were -1.51 to 1.42 and the ICC was 0 .61 (95%confidence interval CI: 0.53-0.68). Calculations using the lower lim it of the 95%CI showed that three administrations of the survey would be requir ed to obtain a more desirable ICC (0.70). The κstatistic for reliability of dry eye disease classification was 0.58 (95%CI: 0.48-0.67). Logistic regression s howed a significant interaction between gender (females) and younger age (P=0.02 ) in relation to misclassification of self-reported dry eye disease status. CON CLUSIONS. The reliability of selfreported dry eye disease classification in cont act lens wearers is moderate. In epidemiologic studies of factors associated wit h self-reported disease status, investigators may be well advised to consider u sing multiple administers of such outcome instruments and controlling for sociod emographic characteristics to maintain internal validity.展开更多
文摘Methylchloroisothiazolinone and methylisothiazolinone (MCI/ MI) contact allergy affects 1-3%of patch-tested patients in European centres. The aim of the present study was to evaluate the importance of the factors -time and concentration (dose/per unit area) -in the elicitation capacity by means of a repeated open application test (ROAT) experimental design. The study was designed as a double-blind, placebo-controlled, dose-response ROAT preceded by a diagnostic patch testing. 25 subjects with confirmed MCI/MI allergy and 10 healthy, non-MCI/MI allergic control subjects were exposed to 0.025 μg/cm2 (2 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. After a wash-out period of at least 4 weeks, the subjects were exposed to 0.094 μg/cm2 (7.5 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. The study showed the importance of both time and exposure in the elicitation process. It demonstrated that the elicitation threshold for MCI/MI is expected to be in the proximity of 0.025 μg/cm2 although it was not possible to establish a definitive elicitation threshold for MCI/MI in this study.
文摘PURPOSE. To evaluate the reliability of self-reported dry eye disease status and patient-related predictors of misclassification in contact lens wearers. ME THODS. Patients completed the Contact Lens Dry Eye Questionnaire (CLDEQ) short form on two occ asions. Test-retest reliability of the CLDEQ composite score was determined usi ng the 95%limits of agreement (LoA) and an intraclass correlation coefficient ( ICC). The κstatistic was used to determine reliability of disease-classificati on-based CLDEQ composite score cutoff points. Predictors of misclassification w ere determined by multivariate logistic regression. RESULTS. The sample included 274 patients. The range of CLDEQ composite scores from both visits was -1.83 t o 4.50 and the mean difference between administrations was -0.05±0.75 (P=0.30) . The 95%LoA of the CLDEQ composite score were -1.51 to 1.42 and the ICC was 0 .61 (95%confidence interval CI: 0.53-0.68). Calculations using the lower lim it of the 95%CI showed that three administrations of the survey would be requir ed to obtain a more desirable ICC (0.70). The κstatistic for reliability of dry eye disease classification was 0.58 (95%CI: 0.48-0.67). Logistic regression s howed a significant interaction between gender (females) and younger age (P=0.02 ) in relation to misclassification of self-reported dry eye disease status. CON CLUSIONS. The reliability of selfreported dry eye disease classification in cont act lens wearers is moderate. In epidemiologic studies of factors associated wit h self-reported disease status, investigators may be well advised to consider u sing multiple administers of such outcome instruments and controlling for sociod emographic characteristics to maintain internal validity.
