The technique of optical tweezers has been improved a lot since its invention, which extends the application fields of optical tweezers. Besides the conventionally used Gaussian beams, different types of ring beams ha...The technique of optical tweezers has been improved a lot since its invention, which extends the application fields of optical tweezers. Besides the conventionally used Gaussian beams, different types of ring beams have also been used to form optical tweezers for different purposes. The two typical kinds of ring beams used in optical tweezers are the hollow Gaussian beam and Laguerre-Gaussian （LG） beam. Both theoretical computation and experiments have shown that the axial trapping force is improved for the ring beams compared with the Gaussian beam, and hence the trapping stability is improved, although the transverse trapping forces of ring beams are smaller than that of Gaussian beam. However, no systematic study on the trapping forces of ring beam has ever been discussed. In this article, we will investigate the axial and transverse trapping forces of different types of ring beams with different parameters systematically, by numerical computation in which the ray optics model is adopted. The spherical aberration caused by the refractive index mismatch between oil and water is also considered in the article. The trapping forces for different objectives that obey the sine condition and tangent condition are also compared with each other. The result of systematical calculation will be useful for the applications of optical tweezers formed by different types of ring beams.展开更多
A dual optical tweezers system, which consists of a doughnut mode optical tweezer （DMOT） with the azimuthally polarised trapping beam and a solid mode optical tweezer （SMOT） with the Gauss trapping beam was constr...A dual optical tweezers system, which consists of a doughnut mode optical tweezer （DMOT） with the azimuthally polarised trapping beam and a solid mode optical tweezer （SMOT） with the Gauss trapping beam was constructed to compare the axial trapping effect of DMOT and SMOT. The long-distance axial trapping of ST68 microbubbles （MBs） achieved by DMOT was more stable than that of SMOT. Moreover the axial trapping force measured using the viscous drag method, was depended on the diameter of the particle, the laser power, and the numerical aperture （NA） of the objective lens. The measurement of the axial trapping force and the acquisition of CCD images of trapping effect confirmed that the DMOT showed excellent axial trapping ability than SMOT. A simple and effective method is developed to improve axial trapping effect using the azimuthally polarized beam as trapping beam. This is helpful for the long-distance manipulating of particles especially polarised biological objects in axial direction.展开更多
Acoustical tweezer is a primary application of the radiation force of a sound field. When an ultrasound focused beam passes through a micro-particle, like a cell or living biological specimens, the particle will be ma...Acoustical tweezer is a primary application of the radiation force of a sound field. When an ultrasound focused beam passes through a micro-particle, like a cell or living biological specimens, the particle will be manipulated accurately without physical contact and invasion, due to the three-dimensional acoustical trapping force. Based on the Ray acoustics approach in the Mie regime, this work discusses the effects on the particle caused by Gaussian focused ultrasound, studies the acoustical trapping force of spherical Mie particles by ultrasound in any position, and analyzes the numerical calculation on the two-dimensional acoustical radiation force. This article also analyzes the conditions for the acoustical trapping phenomenon, and discusses the impact of the initial position and size of the particle on the magnitude of the acoustical radiation force. Furthermore, this paper considers the ultrasonic attenuation in a particle in the case of two-dimension, studies the attenuation's effects on the acoustical trapping force, and amends the calculation to the ordinary case with attenuation.展开更多
An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample cham...An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample chamber and a costly automatism stage, thus the experimental setup is simpler and cheaper. Experiments indicate that the range of the trap force measured by this method is piconewton and sub-piconewton, which makes it fit for study on non-damage interaction between light and biological particles with optical tweezers especially. Since this method is relevant to particle electric charge, by applying an alternating electric field, the new method may overcome the problem of correcting drag force and allow us to measure simultaneously optical trap stiffness and particle electric charge.展开更多
The dynamic behavior of octahedral gold nanopartides （NPs） and nanoparticle clusters （NPCs） in aqueous solution is studied by in-situ liquid-cell transmission electron microscopy （TEM）. The octahedral Au NPs/NPC...The dynamic behavior of octahedral gold nanopartides （NPs） and nanoparticle clusters （NPCs） in aqueous solution is studied by in-situ liquid-cell transmission electron microscopy （TEM）. The octahedral Au NPs/NPCs show preferential orientations in the liquid cell, due to the interaction with the SiNx window. The Au NPs show long-range reversible hopping and three-dimensional （3D） rotational motions in the liquid environment. At the same time, the Au NPCs and NPs perform slow stick-sUp and stick-roU motions, respectivel~ with a centripetal trend. The centripetal motions were explained by a liquid evaporation-induced radial flow model in which the NPCs/NPs trajectories are controlled by Stokes forces and surface friction by the silicon nitride window. The calculated radius-dependent force （Fc） on the NPCs/NPs shows a semi-linear correlation with the distance r between the NPCs/NPs and the center of mass, accompanied with stochastic fluctuations, in agreement with the model predictions. This work thus demonstrates the effectiveness of in situ liquid-cell TEM for the in-depth understanding of complicated liquid flow and force interactions in nanomaterials.展开更多
基金Project supported by the National Natural Science Foundation of China （Grant Nos 20273065 and 10474094） and ‘the Knowledge Innovation Program＇ of Chinese Academy of Sciences.
文摘The technique of optical tweezers has been improved a lot since its invention, which extends the application fields of optical tweezers. Besides the conventionally used Gaussian beams, different types of ring beams have also been used to form optical tweezers for different purposes. The two typical kinds of ring beams used in optical tweezers are the hollow Gaussian beam and Laguerre-Gaussian （LG） beam. Both theoretical computation and experiments have shown that the axial trapping force is improved for the ring beams compared with the Gaussian beam, and hence the trapping stability is improved, although the transverse trapping forces of ring beams are smaller than that of Gaussian beam. However, no systematic study on the trapping forces of ring beam has ever been discussed. In this article, we will investigate the axial and transverse trapping forces of different types of ring beams with different parameters systematically, by numerical computation in which the ray optics model is adopted. The spherical aberration caused by the refractive index mismatch between oil and water is also considered in the article. The trapping forces for different objectives that obey the sine condition and tangent condition are also compared with each other. The result of systematical calculation will be useful for the applications of optical tweezers formed by different types of ring beams.
基金Project supported by the National Natural Science Foundation of China (Grant No. 10674037)the National Basic Research Program of China (Grant No. 2007CB307001)the program of excellent Team in Harbin Institute of Technology of China
文摘A dual optical tweezers system, which consists of a doughnut mode optical tweezer （DMOT） with the azimuthally polarised trapping beam and a solid mode optical tweezer （SMOT） with the Gauss trapping beam was constructed to compare the axial trapping effect of DMOT and SMOT. The long-distance axial trapping of ST68 microbubbles （MBs） achieved by DMOT was more stable than that of SMOT. Moreover the axial trapping force measured using the viscous drag method, was depended on the diameter of the particle, the laser power, and the numerical aperture （NA） of the objective lens. The measurement of the axial trapping force and the acquisition of CCD images of trapping effect confirmed that the DMOT showed excellent axial trapping ability than SMOT. A simple and effective method is developed to improve axial trapping effect using the azimuthally polarized beam as trapping beam. This is helpful for the long-distance manipulating of particles especially polarised biological objects in axial direction.
基金supported by the National Basic Research Program of China(Grant Nos. 2012CB921504, 2011CB707902)the National Natural Science Foundation of China(Grant No. 11274166)+3 种基金Fundamental Research Funds for the Central Universities(Grant Nos. 1113020403,1101020402)the State Key Laboratory of Acoustics, Chinese Academy of Sciences(Grant No. SKLA201207)the priority academic program development of Jiangsu Higher Education Institutions and SRF for ROCS, SEMproject of Interdisciplinary Center of Nanjing University
文摘Acoustical tweezer is a primary application of the radiation force of a sound field. When an ultrasound focused beam passes through a micro-particle, like a cell or living biological specimens, the particle will be manipulated accurately without physical contact and invasion, due to the three-dimensional acoustical trapping force. Based on the Ray acoustics approach in the Mie regime, this work discusses the effects on the particle caused by Gaussian focused ultrasound, studies the acoustical trapping force of spherical Mie particles by ultrasound in any position, and analyzes the numerical calculation on the two-dimensional acoustical radiation force. This article also analyzes the conditions for the acoustical trapping phenomenon, and discusses the impact of the initial position and size of the particle on the magnitude of the acoustical radiation force. Furthermore, this paper considers the ultrasonic attenuation in a particle in the case of two-dimension, studies the attenuation's effects on the acoustical trapping force, and amends the calculation to the ordinary case with attenuation.
基金This work was supported by the National Natural Science Foundation of China under Grant No. 60378018 and 60578026.
文摘An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system （ESCS） is designed instead of a common sample chamber and a costly automatism stage, thus the experimental setup is simpler and cheaper. Experiments indicate that the range of the trap force measured by this method is piconewton and sub-piconewton, which makes it fit for study on non-damage interaction between light and biological particles with optical tweezers especially. Since this method is relevant to particle electric charge, by applying an alternating electric field, the new method may overcome the problem of correcting drag force and allow us to measure simultaneously optical trap stiffness and particle electric charge.
文摘The dynamic behavior of octahedral gold nanopartides （NPs） and nanoparticle clusters （NPCs） in aqueous solution is studied by in-situ liquid-cell transmission electron microscopy （TEM）. The octahedral Au NPs/NPCs show preferential orientations in the liquid cell, due to the interaction with the SiNx window. The Au NPs show long-range reversible hopping and three-dimensional （3D） rotational motions in the liquid environment. At the same time, the Au NPCs and NPs perform slow stick-sUp and stick-roU motions, respectivel~ with a centripetal trend. The centripetal motions were explained by a liquid evaporation-induced radial flow model in which the NPCs/NPs trajectories are controlled by Stokes forces and surface friction by the silicon nitride window. The calculated radius-dependent force （Fc） on the NPCs/NPs shows a semi-linear correlation with the distance r between the NPCs/NPs and the center of mass, accompanied with stochastic fluctuations, in agreement with the model predictions. This work thus demonstrates the effectiveness of in situ liquid-cell TEM for the in-depth understanding of complicated liquid flow and force interactions in nanomaterials.
