Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N...Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N response makes it difficult to predict and quantify the effect of water deficit on crop N status.The nitrogen nutrition index(NNI)has been widely used to accurately diagnose crop N status and to evaluate the effectiveness of N application.The decline of NNI under water-limiting conditions has been documented,although the underlying mechanism governing this decline is not fully understood.This study aimed to elucidate the reason for the decline of NNI under waterlimiting conditions and to provide insights into the accurate utilization of NNI for assessing crop N status under different water-N interaction treatments.Rainout shelter experiments were conducted over three growing seasons from 2018 to 2021 under different N(75 and 225 kg N ha^(-1),low N and high N)and water(120 to 510 mm,W0 to W3)co-limitation treatments.Plant N accumulation,shoot biomass(SB),plant N concentration(%N),soil nitrate-N content,actual evapotranspiration(ET_a),and yield were recorded at the stem elongation,booting,anthesis and grain filling stages.Compared to W0,W1 to W3 treatments exhibited NNI values that were greater by 10.2 to 20.5%,12.6to 24.8%,14 to 24.8%,and 16.8 to 24.8%at stem elongation,booting,anthesis,and grain filling,respectively,across the 2018-2021 seasons.This decline in NNI under water-limiting conditions stemmed from two main factors.First,reduced ET_(a) and SB led to a greater critical N concentration(%N_(c))under water-limiting conditions,which contributed to the decline in NNI primarily under high N conditions.Second,changes in plant%N played a more significant role under low N conditions.Plant N accumulation exhibited a positive allometric relationship with SB and a negative relationship with soil nitrate-N content under water-limiting conditions,indicating co-regulation by SB and the soil nitrate-N content.However,this regulation was influenced by water availability.Plant N accumulation sourced from the soil nitrate-N content reflects soil N availability.Greater soil water availability facilitated greater absorption of soil nitrate-N into the plants,leading to a positive correlation between plant N accumulation and ET_(a)across the different water-N interaction treatments.Therefore,considering the impact of soil water availability is crucial when assessing soil N availability under water-limiting conditions.The findings of this study provide valuable insights into the factors contributing to the decline in NNI among different water-N interaction treatments and can contribute to the more accurate utilization of NNI for assessing winter wheat N status.展开更多
Excessive nitrogen (N) fertilizer application to winter wheat is a common problem on the North China Plain. To determine the optimum fertilizer N rate for winter wheat production while minimizing N losses, field exper...Excessive nitrogen (N) fertilizer application to winter wheat is a common problem on the North China Plain. To determine the optimum fertilizer N rate for winter wheat production while minimizing N losses, field experiments were conducted for two growing seasons at eight sites, in Huimin County, Shandong Province, from 2001 to 2003. The optimum N rate for maximum grain yield was inversely related to the initial soil mineral N content (Nmin) in the top 90 cm of the soil profile before sowing. There was no yield response to the applied N at the three sites with high initial soil mineral N levels (average 212 kg N ha-1). The average optimum N rate was 96 kg N ha-1 for the five sites with low initial soil Nmin (average 155 kg N ha-1) before sowing. Residual nitrate N in the top 90 cm of the soil profile after harvest increased with increasing fertilizer N application rate. The apparent N losses during the wheat-growing season also increased with increasing N application rate. The average apparent N losses with the optimum N rates were less than 15 kg N ha-1, whereas the farmers' conventional N application rate resulted in losses of more than 100 kg N ha-1. Therefore, optimizing N use for winter wheat considerably reduced N losses to the environment without compromising crop yields.展开更多
基金supported by the National Natural Science Foundation of China(51609247)the Henan Provincial Natural Science Foundation,China(222300420589,202300410553)+4 种基金the Central Public-interest Scientific Institution Basal Research Fund,China(FIRI2022-22)the Science&Technology Fundamental Resources Investigation Program,China(2022FY101601)the Science and Technology Project of Xinxiang City,Henan Province,China(GG2021024)the Major Special Science and Technology Project of Henan Province,China(221100110700)the Joint Fund of Science and Technology Research and Development Plan of Henan Province,China(Superior Discipline Cultivation)(222301420104)。
文摘Nitrogen(N)uptake is regulated by water availability,and a water deficit can limit crop N responses by reducing N uptake and utilization.The complex and multifaceted interplay between water availability and the crop N response makes it difficult to predict and quantify the effect of water deficit on crop N status.The nitrogen nutrition index(NNI)has been widely used to accurately diagnose crop N status and to evaluate the effectiveness of N application.The decline of NNI under water-limiting conditions has been documented,although the underlying mechanism governing this decline is not fully understood.This study aimed to elucidate the reason for the decline of NNI under waterlimiting conditions and to provide insights into the accurate utilization of NNI for assessing crop N status under different water-N interaction treatments.Rainout shelter experiments were conducted over three growing seasons from 2018 to 2021 under different N(75 and 225 kg N ha^(-1),low N and high N)and water(120 to 510 mm,W0 to W3)co-limitation treatments.Plant N accumulation,shoot biomass(SB),plant N concentration(%N),soil nitrate-N content,actual evapotranspiration(ET_a),and yield were recorded at the stem elongation,booting,anthesis and grain filling stages.Compared to W0,W1 to W3 treatments exhibited NNI values that were greater by 10.2 to 20.5%,12.6to 24.8%,14 to 24.8%,and 16.8 to 24.8%at stem elongation,booting,anthesis,and grain filling,respectively,across the 2018-2021 seasons.This decline in NNI under water-limiting conditions stemmed from two main factors.First,reduced ET_(a) and SB led to a greater critical N concentration(%N_(c))under water-limiting conditions,which contributed to the decline in NNI primarily under high N conditions.Second,changes in plant%N played a more significant role under low N conditions.Plant N accumulation exhibited a positive allometric relationship with SB and a negative relationship with soil nitrate-N content under water-limiting conditions,indicating co-regulation by SB and the soil nitrate-N content.However,this regulation was influenced by water availability.Plant N accumulation sourced from the soil nitrate-N content reflects soil N availability.Greater soil water availability facilitated greater absorption of soil nitrate-N into the plants,leading to a positive correlation between plant N accumulation and ET_(a)across the different water-N interaction treatments.Therefore,considering the impact of soil water availability is crucial when assessing soil N availability under water-limiting conditions.The findings of this study provide valuable insights into the factors contributing to the decline in NNI among different water-N interaction treatments and can contribute to the more accurate utilization of NNI for assessing winter wheat N status.
基金Project supported by the National Natural Science Foundation of China (Nos. 30390084 and 30270772)the Natural Science Foundation of Beijing (No. 6010001)
文摘Excessive nitrogen (N) fertilizer application to winter wheat is a common problem on the North China Plain. To determine the optimum fertilizer N rate for winter wheat production while minimizing N losses, field experiments were conducted for two growing seasons at eight sites, in Huimin County, Shandong Province, from 2001 to 2003. The optimum N rate for maximum grain yield was inversely related to the initial soil mineral N content (Nmin) in the top 90 cm of the soil profile before sowing. There was no yield response to the applied N at the three sites with high initial soil mineral N levels (average 212 kg N ha-1). The average optimum N rate was 96 kg N ha-1 for the five sites with low initial soil Nmin (average 155 kg N ha-1) before sowing. Residual nitrate N in the top 90 cm of the soil profile after harvest increased with increasing fertilizer N application rate. The apparent N losses during the wheat-growing season also increased with increasing N application rate. The average apparent N losses with the optimum N rates were less than 15 kg N ha-1, whereas the farmers' conventional N application rate resulted in losses of more than 100 kg N ha-1. Therefore, optimizing N use for winter wheat considerably reduced N losses to the environment without compromising crop yields.
