ObjectiveTo investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines.MethodsIn vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231)...ObjectiveTo investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines.MethodsIn vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates.ResultsOf the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82) μg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 μg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246, GQ478247, GQ478248, GQ478249 and GQ478250, respectively. The phylogenetic tree analysis showed that, the isolates of ACT02 and ACT03 were represented in group I and III, respectively, but ACT01 and ACT02 were represented in group II. The multiple sequence alignment of the actinomycete isolates showed that, the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs, 65 to 119th base pairs and 55, 48 and 31st base pairs. Secondary structure prediction of the 16s rRNA showed that, the maximum free energy was consumed with ACT03 isolate (-45.4 kkal/mol) and the minimum free energy was consumed with ACT04 isolate (?7.6 kkal/mol).ConclusionsThe actinomycete isolates of ACT01 and ACT02 (GQ478246 and GQ478247) which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.展开更多
This study systematically investigated the effects of a low-intensity magnetic field on the influence of keratinase activity, peptide yield, and structural and functional properties of peptides produced during solid-s...This study systematically investigated the effects of a low-intensity magnetic field on the influence of keratinase activity, peptide yield, and structural and functional properties of peptides produced during solid-state fermentation (SSF) of mixed organic substrates (chicken feather powder and okara) using a mutant strain of Bacillus licheniformis. Initially, the magnetic field-assisted SSF (MSSF) conditions were optimized, which provided the optimized conditions as the number of treatments 3 at every 24 h (24, 48, and 72 h) with 4 h holding time at 120 Gs of magnetic intensity (mI). Under the optimum conditions, keratinase activity and peptide production increased by 10.31% and 13.77%, respectively. Further, in order to evaluate the influence of magnetic field treatment on the peptides, MSSF experiments were done under different mI conditions (40, 80, 120, and 160 Gs), followed by the evaluation of the structural changes of the extracted peptides. The structural analysis revealed that mI had a significant impact on the keratin surface. In contrast, secondary structure analysis confirmed the unfolding of the peptide with decreased α-keratin and increased β-keratin, thereby boosting the bioactive properties of the peptides. The highest hydroxyl free radical (.OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and Fe2+ chelating rates (56.55, 71.36, and 50.72%, respectively) were found at 120 Gs, which were insignificant with the results at 160 Gs. Therefore, MSSF has a positive effect on the proteolysis mechanism, which can increase bioactive peptide production from keratin.展开更多
基金supported by Indian Council of Medical Research,New Delhi(grant No.59/6/200/BMS/TRM)
文摘ObjectiveTo investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines.MethodsIn vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates.ResultsOf the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82) μg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 μg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246, GQ478247, GQ478248, GQ478249 and GQ478250, respectively. The phylogenetic tree analysis showed that, the isolates of ACT02 and ACT03 were represented in group I and III, respectively, but ACT01 and ACT02 were represented in group II. The multiple sequence alignment of the actinomycete isolates showed that, the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs, 65 to 119th base pairs and 55, 48 and 31st base pairs. Secondary structure prediction of the 16s rRNA showed that, the maximum free energy was consumed with ACT03 isolate (-45.4 kkal/mol) and the minimum free energy was consumed with ACT04 isolate (?7.6 kkal/mol).ConclusionsThe actinomycete isolates of ACT01 and ACT02 (GQ478246 and GQ478247) which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.
基金the National Natural Science Foundation of China(Grant No.:31601516)for the financial support.
文摘This study systematically investigated the effects of a low-intensity magnetic field on the influence of keratinase activity, peptide yield, and structural and functional properties of peptides produced during solid-state fermentation (SSF) of mixed organic substrates (chicken feather powder and okara) using a mutant strain of Bacillus licheniformis. Initially, the magnetic field-assisted SSF (MSSF) conditions were optimized, which provided the optimized conditions as the number of treatments 3 at every 24 h (24, 48, and 72 h) with 4 h holding time at 120 Gs of magnetic intensity (mI). Under the optimum conditions, keratinase activity and peptide production increased by 10.31% and 13.77%, respectively. Further, in order to evaluate the influence of magnetic field treatment on the peptides, MSSF experiments were done under different mI conditions (40, 80, 120, and 160 Gs), followed by the evaluation of the structural changes of the extracted peptides. The structural analysis revealed that mI had a significant impact on the keratin surface. In contrast, secondary structure analysis confirmed the unfolding of the peptide with decreased α-keratin and increased β-keratin, thereby boosting the bioactive properties of the peptides. The highest hydroxyl free radical (.OH), 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and Fe2+ chelating rates (56.55, 71.36, and 50.72%, respectively) were found at 120 Gs, which were insignificant with the results at 160 Gs. Therefore, MSSF has a positive effect on the proteolysis mechanism, which can increase bioactive peptide production from keratin.
