Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to chara...Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.展开更多
The Au nanoparticles has been prepared by microwave high-pressure procedure with alcohol as the reducing agent. The color of colloidal Au nanoparticles is blue-violet. The maximum absorption spectrum of colloidal Au i...The Au nanoparticles has been prepared by microwave high-pressure procedure with alcohol as the reducing agent. The color of colloidal Au nanoparticles is blue-violet. The maximum absorption spectrum of colloidal Au is at 580 nm, and the resonance scattering peak is at 580 nm. Using this method, the colloidal Au of long-time stability can be prepared Simply and quickly.展开更多
A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×...A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×10 -3 mol/L), HSA may combine with 3- by intermolecular forces (mainly by electrostatic force) to form { 3- n-HSA m+} k nanoparticle of the ion-association complexes of HSA m+- 3- n. There is a strongest resonance scattering intensity at 351 nm, owing to the existence of the resonance scattering of the nanoparticle, 3- molecular absorption and the non-distribution of the emission intensity of Xe lamp in the range of 200-1000 nm. In addition, two resonance scattering peaks at 470 and 700 nm were observed. The HSA concentration in the range of 0-12 μg/mL is linear to the resonance scattering intensity at 351 nm. The determination limit of this method is 0.1 μg/mL HSA, about ten-fold lower than that of Coomassie brilliant blue protein assay. This method has been used for the determination of HSA in human serum and synthetic samples with satisfactory results. The mechanism of enhanced resonance scattering light, the TEM of the particle, the concepts of quasi-elastic absorption and un-elastic absorption were also discussed.展开更多
基金financially supported by the National Natural Science Foundation of China(No.20975067)RFDP (No.20090073120039)Shanghai Educational Development Foundation(No.2008CG12)
文摘Gold nanoparticles （GNPs） have been widely used as probes and nanomaterials in certain biological and biomedical fields thanks to its special physical and chemical properties. However, it is still difficult to characterize GNPs-bioconjugates in solution, which has greatly limited further bioapplications of GNPs. In this study, we reported a single particle method for characterizing GNPs- biomolecules in solution using resonance light scattering correlation spectroscopy （RLSCS）. The interaction of GNPs with bovine serum albumin （BSA） and thiol-modified oligonucletides were investigated.
文摘The Au nanoparticles has been prepared by microwave high-pressure procedure with alcohol as the reducing agent. The color of colloidal Au nanoparticles is blue-violet. The maximum absorption spectrum of colloidal Au is at 580 nm, and the resonance scattering peak is at 580 nm. Using this method, the colloidal Au of long-time stability can be prepared Simply and quickly.
文摘A new resonance scattering spectral (RSS) method for the determination of human serum album (HSA) has been proposed with the resonance scattering enhanced reagent of K 3[Fe-(CN) 6]. In the medium of HCl (2.0×10 -3 mol/L), HSA may combine with 3- by intermolecular forces (mainly by electrostatic force) to form { 3- n-HSA m+} k nanoparticle of the ion-association complexes of HSA m+- 3- n. There is a strongest resonance scattering intensity at 351 nm, owing to the existence of the resonance scattering of the nanoparticle, 3- molecular absorption and the non-distribution of the emission intensity of Xe lamp in the range of 200-1000 nm. In addition, two resonance scattering peaks at 470 and 700 nm were observed. The HSA concentration in the range of 0-12 μg/mL is linear to the resonance scattering intensity at 351 nm. The determination limit of this method is 0.1 μg/mL HSA, about ten-fold lower than that of Coomassie brilliant blue protein assay. This method has been used for the determination of HSA in human serum and synthetic samples with satisfactory results. The mechanism of enhanced resonance scattering light, the TEM of the particle, the concepts of quasi-elastic absorption and un-elastic absorption were also discussed.
