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PCR-DGGE Analysis of Bacterial Communities Structure in Babylonia areolata Culture Systems of The Subtidal Zone and The Pond Mulched Plastic Film and Sand in Bottom
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作者 李淑芳 邱德全 +2 位作者 张继东 杨世平 邱明生 《Agricultural Science & Technology》 CAS 2015年第7期1535-1539,1543,共6页
To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in ... To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in bottom, the bacte- rial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis (DGGE). The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobac- teda Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Garnmaproteobacteria, Alphaproteobacteria, Deltaprotecbacteda, Epsilonproteobacteda, Anaerolineae, Cyanobacteria and Acti- nobacteda. The dominant bacterial communities in the subtidal zone culture system were Gammaprotecbacteda, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteda, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture sys- tems. The results of unweighted pair group method with arithmetic average (UPG- MA) showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5%. The bacterial communities of seawater samples S3 and S4 in the above culture systems were in clusters, and the similarity of the bacterial communi- ties was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the sub-tidal zone culture systems through changing the pond seawater and monitoring the microbial population. 展开更多
关键词 Bacterial communities structure Denaturing gradient gel electrophoresis (DGGE) Culture system of the sub-tidal zone Culture system of the pond mulched plastic film and sand Babylonia areolata
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Influential factors on the repose angle of desert sands
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作者 JianBao Liu NaiAng Wang +1 位作者 HongYi Cheng WenTao Yang 《Research in Cold and Arid Regions》 2011年第1期17-23,共7页
The repose angle is one of the most significant macroscopic parameters in describing the behavior of granular materials. Under a static condition, the repose angle is the steepest angle at which sediment particles can... The repose angle is one of the most significant macroscopic parameters in describing the behavior of granular materials. Under a static condition, the repose angle is the steepest angle at which sediment particles can rest without motion. In this paper, we use existing data and aeolian physics to analyze the main factors that influence the repose angle of sand dunes, and we investigate different repose angles involving various states and types of materials. We have determined that different factors have differential influence on the magnitude of the repose angle. Our results show that for powdery (〈400-μm diameter) desert sands, the main influential factor on the magnitude of repose angle is the molecular force among particles. Particle size does not influence the repose angle of desert sands directly, but has an indirect impact by affecting the grit sphericity and surface roughness, of which the grit sphericity acts as a major factor. Even at the same average particle size, the repose angle differs with different grain compositions. Furthermore, with increasing unevenness in grain composition, the repose angle increases correspondingly. Sand texture also has a direct influence on the repose angle of desert sands. In two sand samples having the same grain composition but different textures, the repose angles may be different. Water content has a stronger influence on the repose angle than any other factor. However, the relationship between the repose angle and water content is not a simple direct proportion. In fact, with increasing water content, the repose angle first increases and then decreases. These research results will be useful for understanding the mechanisms of dune transport, variations of dune morphology, and the stability and fluidity of dune sands. 展开更多
关键词 repose angle desert sands plastic sands uniformity coefficient water content
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