This study primarily investigates the rock fracture mechanism of bottom cushion layer blasting and explores the effects of the bottom cushion layer on rock fragmentation.It involves analyses of the evolution patterns ...This study primarily investigates the rock fracture mechanism of bottom cushion layer blasting and explores the effects of the bottom cushion layer on rock fragmentation.It involves analyses of the evolution patterns of blasting stress,characteristics of crack distribution,and rock fracture features in the specimens.First,blasting model experiments were carried out using the dynamic caustics principle to investigate the influence of bottom cushion layers and initiation methods on the integrity of the bottom rock mass.The experimental results indicate that the combined use of bottom cushion layers and inverse initiation effectively protects the integrity of the bottom rock mass.Subsequently,the process of stress wave propagation and dynamic crack propagation in rocks was simulated using the continuum-discontinuum element method(CDEM)and the Landau explosion source model,with varying thicknesses of bottom cushion layers.The numerical simulation results indicate that with increasing cushion thickness,the absorption of energy generated by the explosion becomes more pronounced,resulting in fewer cracks in the bottom rock mass.This illustrates the positive role of the cushion layer in protecting the integrity of the bottom rock mass.展开更多
The axial uncoupling coefficient and air deck effect in blasting significantly influence the effectiveness of rock fragmentation.This study employs a passive confinement device to conduct continuous charge and five di...The axial uncoupling coefficient and air deck effect in blasting significantly influence the effectiveness of rock fragmentation.This study employs a passive confinement device to conduct continuous charge and five different axial uncoupling coefficient blasting ex-periments on cylindrical iron ore samples to explain the rock-breaking mechanisms associated with various axial uncoupling coefficientsand air deck effects. It utilizes advanced techniques such as computer tomography(CT) scanning, deep learning, and three dimensional(3D) model reconstruction, to generate a 3D reconstruction model of “rock explosion cracks” under varying axial uncoupling coefficients.This model illustrates the spatial distribution and configurations of explosion cracks. Integrating box-counting dimension and fractal di-mension theories enables the quantitative analysis of the three-dimensional fracture field and the extent of damage in rocks subjected toexplosive forces. Laboratory 3D experimental results indicate that continuous charging produces the most extensive damage, while a un-coupling coefficient of 1.50(case 1) results in the least. A moderate air deck length enhances blasting effectiveness and rock fragmenta-tion. For identical charge quantities. In contrast, increasing the charge amount with a constant air deck length further augments rock frag-mentation. A rock blasting calculation model was developed using LS-DYNA numerical simulation software under various axial uncoup-ling coefficients. This model depicts the dynamic damage evolution characteristics of the rocks and variations in hole wall pressure. Thenumerical simulation results of cumulative rock damage align with the laboratory findings. In addition, increasing the air deck length re-duces the peak of the explosion shock wave, decreasing the peak pressure in the charge and air sections by 37.8% to 66.3%. These re-search outcomes provide valuable theoretical support for designing and optimizing axial uncoupling coefficients in practical applications.展开更多
Cross roadway collapses are a common occurrence in underground mining operations.While the influence of mining blasts on the stability of surrounding rock is acknowledged,the underlying mechanisms remain inadequately ...Cross roadway collapses are a common occurrence in underground mining operations.While the influence of mining blasts on the stability of surrounding rock is acknowledged,the underlying mechanisms remain inadequately understood.This study investigates the characteristics and mechanisms of collapse in a shallow buried cross roadway subjected to mining blast disturbances,drawing insights from an engineering project in Anshan City,Northeast China.A strain-softening model based on unified strength theory was developed to effectively calculate and analyze the loosened zone thickness and surrounding rock displacement.The PFC3D-FLAC3D coupling method was employed to clarify the concentrated collapse area within the cross roadway,providing insight into the collapse mechanism through a cross-sectional model of the concentrated region.Results demonstrate that 50%of the cross roadway collapsed following the mining blast.Subsidence at the intersection was approximately one-fifth(0.66 m)of cross roadway’s net height,exceeding subsidence in other areas by 1.3.Under the action of repeated mining blasting,the cross section of the connection roadway forms a semi-elliptical high tensile stress zone.After the cumulative damage of the surrounding rock of the connection roadway exceeds the ultimate yield strength,the cumulative stress release causes the tensile failure of the surrounding rock.The plastic zone of the connecting roadway expands to three times of the initial,and continues to develop.The surrounding rock on both sides experienced tensile stress,cumulative stress release,and the vertical propagation of tensile cracks.展开更多
In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal ...In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal masses produce many irreversible cracks.Afterblasting,the nearer the distance from blasting hole,the larger the BET surface areaand volume ratio of the infiltration pore are;they increased by 11.47%and 5.73%,respectively.The coefficient of air permeability is increased 4 times.After 3 months,the gasdrainage rate was increased by 66%.In the first 15 days,the cumulative pumped gas was1.93 times of blasting before.The average absolute gas emission decreased by 63.46%.Experimental results show that deep-hole controlled pre-splitting blasting not only preventscoal and gas outburst,but also gives good economic results.展开更多
Coalbed gas extraction is an important means of exploiting and utilizing gas resources,as well as a means of preventing coal mine disasters.In view of the low gas extraction rate from coalbeds with high gas content an...Coalbed gas extraction is an important means of exploiting and utilizing gas resources,as well as a means of preventing coal mine disasters.In view of the low gas extraction rate from coalbeds with high gas content and low permeability,a method of improving permeability through deep-hole cumulative blasting is applied to develop initial directional fractures using a jet flow.Under the action of the blasting stress wave and detonation gas wedge,the fractures extend over a large range within the coal,thereby improving coalbed permeability.This study focuses on the criteria of cumulative blasting-induced coalbed fracturing based on a literature review of the penetration effect of cumulative blasting.On this basis,we summarize the coal fracturing zone,crack extension process,and the key technologies of charging and hole sealing for cumulative blasting.In addition,the latest research progress in the optimization of field test drilling and blasting parameters for cumulative blasting is introduced.Research findings indicate that the permeability improvement mechanism of cumulative blasting could be further enhanced,and the technology and technical equipment are in urgent need of improvement.Finally,development trends in the cumulative blasting permeability improvement technique are identified.展开更多
To study the effects of the initiation position on the damage and fracture characteristics of linear-charge blasting, blasting model experiments were conducted in this study using computed tomography scanning and thre...To study the effects of the initiation position on the damage and fracture characteristics of linear-charge blasting, blasting model experiments were conducted in this study using computed tomography scanning and three-dimensional reconstruction methods. The fractal damage theory was used to quantify the crack distribution and damage degree of sandstone specimens after blasting. The results showed that regardless of an inverse or top initiation, due to compression deformation and sliding frictional resistance, the plugging medium of the borehole is effective. The energy of the explosive gas near the top of the borehole is consumed. This affects the effective crushing of rocks near the top of the borehole, where the extent of damage to Sections Ⅰ and Ⅱ is less than that of Sections Ⅲ and Ⅳ. In addition, the analysis revealed that under conditions of top initiation, the reflected tensile damage of the rock at the free face of the top of the borehole and the compression deformation of the plug and friction consume more blasting energy, resulting in lower blasting energy efficiency for top initiation. As a result, the overall damage degree of the specimens in the top-initiation group was significantly smaller than that in the inverse-initiation group. Under conditions of inverse initiation, the blasting energy efficiency is greater, causing the specimen to experience greater damage. Therefore, in the engineering practice of rock tunnel cut blasting, to utilize blasting energy effectively and enhance the effects of rock fragmentation, using the inverse-initiation method is recommended. In addition, in three-dimensional(3D) rock blasting, the bottom of the borehole has obvious end effects under the conditions of inverse initiation, and the crack distribution at the bottom of the borehole is trumpet-shaped. The occurrence of an end effect in the 3D linear-charge blasting model experiment is related to the initiation position and the blocking condition.展开更多
Stress waves affect the stress field at the crack tip and dominate the dynamic crack propagation.Therefore,evaluating the influence of blasting stress waves on the crack propagation behavior and the mechanical charact...Stress waves affect the stress field at the crack tip and dominate the dynamic crack propagation.Therefore,evaluating the influence of blasting stress waves on the crack propagation behavior and the mechanical characteristics of crack propagation is of great significance for engineering blasting.In this study,ANSYS/LS-DYNA was used for blasting numerical simulation,in which the propagation characteristics of blasting stress waves and stress field distribution at the crack tip were closely observed.Moreover,ABAQUS was applied for simulating the crack propagation path and calculating dynamic stress intensity factors(DSIFs).The universal function was calculated by the fractalmethod.The results show that:the compressive wave causes the crack to close and the reflected tensile wave drives the crack to initiate and propagate,and failure mode is mainly tensile failure.The crack propagation velocity varies with time,which increases at first and then decreases,and the crack arrest occurs due to the attenuation of stress waves and dissipation of the blasting energy.In addition,crack arrest toughness is smaller than the crack initiation toughness,applied pressure waveforms(such as the peak pressure,duration,waveforms,wavelengths and loading rates)have a great influence on DSIFs.It is conducive to our deep understanding or the study of blasting stress waves dominated fracture,suggesting a broad reference for the further development of rock blasting in engineering practice.展开更多
The deformation control of surrounding rock in gobside roadway with thick and hard roof poses a significant challenge to the safety and efficiency of coal mining.To address this issue,a novel approach combining direct...The deformation control of surrounding rock in gobside roadway with thick and hard roof poses a significant challenge to the safety and efficiency of coal mining.To address this issue,a novel approach combining directional and non-directional blasting techniques,known as combined blasting,was proposed.This study focuses on the experimental investigation of the proposed method in the 122108 working face in Caojiatan Coal Mine as the engineering background.The initial phase of the study involves physical model experiments to reveal the underlying mechanisms of combined blasting for protecting gob-side roadway with thick and hard roof.The results demonstrate that this approach effectively accelerates the collapse of thick and hard roofs,enhances the fragmentation and expansion coefficient of gangue,facilitates the filling of the goaf with gangue,and provides support to the overlying strata,thus reducing the subsidence of the overlying strata above the goaf.Additionally,the method involves cutting the main roof into shorter beams to decrease the stress and disrupt stress transmission pathways.Subsequent numerical simulations were conducted to corroborate the findings of the physical model experiments,thus validating the accuracy of the experimental results.Furthermore,field engineering experiments were performed,affirming the efficacy of the combined blasting method in mitigating the deformation of surrounding rock and achieving the desired protection of the gob-side roadway.展开更多
Water decoupling charge blasting excels in rock breaking,relying on its uniform pressure transmission and low energy dissipation.The water decoupling coefficients can adjust the contributions of the stress wave and qu...Water decoupling charge blasting excels in rock breaking,relying on its uniform pressure transmission and low energy dissipation.The water decoupling coefficients can adjust the contributions of the stress wave and quasi-static pressure.However,the quantitative relationship between the two contributions is unclear,and it is difficult to provide reasonable theoretical support for the design of water decoupling blasting.In this study,a theoretical model of blasting fracturing partitioning is established.The mechanical mechanism and determination method of the optimal decoupling coefficient are obtained.The reliability is verified through model experiments and a field test.The results show that with the increasing of decoupling coefficient,the rock breaking ability of blasting dynamic action decreases,while quasi-static action increases and then decreases.The ability of quasi-static action to wedge into cracks changes due to the spatial adjustment of the blast hole and crushed zone.The quasi-static action plays a leading role in the fracturing range,determining an optimal decoupling coefficient.The optimal water decoupling coefficient is not a fixed value,which can be obtained by the proposed theoretical model.Compared with the theoretical results,the maximum error in the model experiment results is 8.03%,and the error in the field test result is 3.04%.展开更多
The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection ...The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection in the field of blasting.Serverless Computing can provide a variety of computing services for people without hardware foundations and rich software development experience,which has aroused people’s interest in how to use it in the field ofmachine learning.In this paper,we design a distributedmachine learning training application based on the AWS Lambda platform.Based on data parallelism,the data aggregation and training synchronization in Function as a Service(FaaS)are effectively realized.It also encrypts the data set,effectively reducing the risk of data leakage.We rent a cloud server and a Lambda,and then we conduct experiments to evaluate our applications.Our results indicate the effectiveness,rapidity,and economy of distributed training on FaaS.展开更多
Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by ...Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.展开更多
Blasting operations,which are crucial to open-pit mine production due to their simplicity and efficiency,require precise control through accurate vibration velocity calculations.The conventional Sadowski formula mainl...Blasting operations,which are crucial to open-pit mine production due to their simplicity and efficiency,require precise control through accurate vibration velocity calculations.The conventional Sadowski formula mainly focuses on blast center distance but neglects the amplification effect of blasting vibration waves by terraced terrain,from which the calculated blasting vibration velocities are smaller than the actual values,affecting the safety of the project.To address this issue,our model introduces the influences of slope and time into Sadowski formula to measure safety through blast vibration displacement.In the northern section of the open-pit quartz mine in Jinchang City,Gansu Province,China,the data of a continuous blasting slope project are referred to.Our findings reveal a noticeable vibration amplification effect during blasting when a multi-stage slope platform undergoes a sudden cross-sectional change near the upper overhanging surface.The amplification vibration coefficient increases with height,while vibration waves within rocks decrease from bottom to top.Conversely,platforms without distinct crosssectional changes exhibit no pronounced amplification during blasting.In addition,the vibration intensity decreases with distance as the rock height difference change propagates.The results obtained by the proposed blast vibration displacement equation incorporating slope shape influence closely agree with real-world scenarios.According to Pearson correlation coefficient(PPMCC)analysis,the average accuracy rate of our model is 88.84%,which exceeds the conventional Sadowski formula(46.92%).展开更多
Accurately estimating blasting vibration during rock blasting is the foundation of blasting vibration management.In this study,Tuna Swarm Optimization(TSO),Whale Optimization Algorithm(WOA),and Cuckoo Search(CS)were u...Accurately estimating blasting vibration during rock blasting is the foundation of blasting vibration management.In this study,Tuna Swarm Optimization(TSO),Whale Optimization Algorithm(WOA),and Cuckoo Search(CS)were used to optimize two hyperparameters in support vector regression(SVR).Based on these methods,three hybrid models to predict peak particle velocity(PPV)for bench blasting were developed.Eighty-eight samples were collected to establish the PPV database,eight initial blasting parameters were chosen as input parameters for the predictionmodel,and the PPV was the output parameter.As predictive performance evaluation indicators,the coefficient of determination(R2),rootmean square error(RMSE),mean absolute error(MAE),and a10-index were selected.The normalizedmutual information value is then used to evaluate the impact of various input parameters on the PPV prediction outcomes.According to the research findings,TSO,WOA,and CS can all enhance the predictive performance of the SVR model.The TSO-SVR model provides the most accurate predictions.The performances of the optimized hybrid SVR models are superior to the unoptimized traditional prediction model.The maximum charge per delay impacts the PPV prediction value the most.展开更多
This study utilizes empirical equations to describe the propagation of vibrations induced by blasting, with the goal of predicting the attenuation of Peak Particle Velocity (PPV) at the Yaramoko mine in Bagassi, Burki...This study utilizes empirical equations to describe the propagation of vibrations induced by blasting, with the goal of predicting the attenuation of Peak Particle Velocity (PPV) at the Yaramoko mine in Bagassi, Burkina Faso, a site characterized by granitoid rock. Four empirical PPV prediction equations were employed, so-called Duvall & Fogelson (or the United States Bureau of Mines “USBM”), Langefors and Kihlstrom, Ambressys-Hendron, and the Bureau of Indian Standard. The constant parameters for each of these equations, referred to as site constants, were derived from linear regression curves. The results show that the site constants k, a, and b of 4762, 0.869, and 1.737, respectively, derived from the general prediction equation by Davies, PPV = kQaD−b, based on Duvall & Fogelson, are in good agreement with values of 4690, 0.9, and 1.69, respectively, for similar rock types in Spain. Regarding the impacts of blasting on houses, the findings indicate that houses built from laterite-block bricks in the village of Bagassi are the most vulnerable to vibration waves, followed by those constructed with cinder-block bricks. In contrast, houses made of banco bricks are the most resilient. Additionally, it was determined that during blasting operations, adjusting the blasting parameters to ensure the PPV does not exceed 2 mm/s at the level of nearby dwellings can minimize the appearance of cracks in houses.展开更多
Integrating liquid CO_(2)phase transition blasting(LCPTB)technology with hydraulic fracturing(HF)methods can help reduce wellbore damage,create multiple radial fractures,and establish a complex fracture network.This a...Integrating liquid CO_(2)phase transition blasting(LCPTB)technology with hydraulic fracturing(HF)methods can help reduce wellbore damage,create multiple radial fractures,and establish a complex fracture network.This approach significantly increases the recovery efficiency of low-permeability oil and gas fields.Accurately calculating the number of fractures caused by LCPTB is necessary to predict production enhancement effects and optimize subsequent HF designs.However,few studies are reported on large-scale physical model experiments in terms of a method for calculating the fracture number.This study analyzed the initiation and propagation of cracks under LCPTB,derived a calculation formula for crack propagation radius under stress waves,and then proposed a new,fast,and accurate method for calculating the fracture number using the principle of mass conservation.Through ten rock-breaking tests using LCPTB,the study confirmed the effectiveness of the proposed calculation approach and elucidated the variation rule of explosion pressure,rock-breaking scenario,and the impact of varying parameters on fracture number.The results show that the new calculation method is suitable for fracturing technologies with high pressure rates.Recommendations include enlarging the diameter of the fracturing tube and increasing the liquid CO_(2) mass in the tube to enhance fracture effectiveness.Moreover,the method can be applied to other fracturing technologies,such as explosive fracturing(EF)within HF formations,indicating its broader applicability and potential impact on optimizing unconventional resource extraction technologies.展开更多
Blasting-induced cracks in the rock surrounding deeply buried tunnels can result in water gushing and rock mass collapse,posing significant safety risks.However,previous theoretical studies on the range of blasting-in...Blasting-induced cracks in the rock surrounding deeply buried tunnels can result in water gushing and rock mass collapse,posing significant safety risks.However,previous theoretical studies on the range of blasting-induced cracks often ignore the impact of the in-situ stress,especially that of the intermediate principal stress.The particle displacement−crack radius relationship was established in this paper by utilizing the blasthole cavity expansion equation,and theoretical analytical formulas of the stress−displacement relationship and the crack radius were derived with unified strength theory to accurately assess the range of cracks in deep surrounding rock under a blasting load.Parameter analysis showed that the crushing zone size was positively correlated with in-situ stress,intermediate principal stress,and detonation pressure,whereas negatively correlated with Poisson ratio and decoupling coefficient.The dilatancy angle-crushing zone size relationship exhibited nonmonotonic behavior.The relationships in the crushing zone and the fracture zone exhibited opposite trends under the influence of only in-situ stress or intermediate principal stress.As the in-situ stress increased from 0 to 70 MPa,the rate of change in the crack range and the attenuation rate of the peak vibration velocity gradually slowed.展开更多
Blast furnace gas(BFG)is an important by-product energy for the iron and steel industry and has been widely used for heating or electricity generation.However,the undesirable contaminants in BFG(especially H_(2)S)gene...Blast furnace gas(BFG)is an important by-product energy for the iron and steel industry and has been widely used for heating or electricity generation.However,the undesirable contaminants in BFG(especially H_(2)S)generate harmful environmental emissions.The desulfurization of BFG is urgent for integrated steel plants due to the stringent ultra-low emission standards.Compared with other desulfurization materials,zeolite-based adsorbents represent a viable option with low costs and long service life.In this study,an ammonia-induced CuO modified 13X adsorbent(NH_(3)–CuO/13X)was prepared for H_(2)S removal from simulated BFG at low temperature.The XRD,H_(2)-TPR and TEM analysis proved that smaller CuO particles were formed and the dispersion of Cu on the surface of 13X zeolite was improved via the induction of ammonia.Evaluation on H_(2)S adsorption performance of the adsorbent was carried out using simulated BFG,and the results showed that NH_(3)–CuO/13X-3 has better breakthrough sulfur capacity,which was more than twice the sulfur capacity of CuO/13X.It is proposed that the enhanced desulfurization performance of NH_(3)–CuO/13X is attributed to an abundant pore of 13X,and combined action of 13X and CuO.This work provided an effective way to improve the sulfur capacity of zeolite-based adsorbents via impregnation method by ammonia induction.展开更多
Asian rice comprises two major subspecies:Xian(X)and Geng(G),and the diverged resistance genes(R)have provided a foundation for breeding improved cultivars to control rice blast disease.After conducting two-phase alle...Asian rice comprises two major subspecies:Xian(X)and Geng(G),and the diverged resistance genes(R)have provided a foundation for breeding improved cultivars to control rice blast disease.After conducting two-phase allele mining using six updated FNP marker systems,the functional haplotypes at Pit,Pib,and Pi63 strictly diverged into the X-populations and were defined as X-R loci,while those at Pi54,Pi37,and Pi36 into the G-populations as G-R loci.The genic diversity at the three X-R loci(16 alleles)was twofold higher than that at the three G-R loci(8 alleles),and the allelic diversity in the Southern region(21 alleles)was nearly double that in the Northeastern region(11 alleles).Both observations reflect a significant difference in genetic diversity between X-and G-populations,and indicate that the effective R-genes mainly originated from X-subspecies.Based on the allelic structures characterized by a set of 10 parameters,8 and 16 alleles were respectively recognized as favorable and promising ones for the regional breeding programs.The genotypic structures of the two regional populations were almost different,indicating that the diverged alleles have been further assembled into two series of regional genotypes through long-term breeding programs,despite the presence of one-third of region-common alleles.The genotypic diversity in the Southern region(55 genotypes)was nearly twice as high as that in the Northeastern region(28),which perfectly reflects the aforementioned differences in both genic and allelic diversities.After analyzing the genotypic structures using a set of 13 parameters,4 and 23 genotypes,respectively,can be recommended as the favorable and promising ones for the regional breeding programs.The case study serves as a concrete sample of how to identify the favorable and promising alleles and genotypes,and beneficial parents based their comprehensive population structures for gene-designed breeding.展开更多
The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature...The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature.Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens.However,only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites.In this study,we conducted a metabolomic analysis on both blast-resistant(Digu)and-susceptible(Lijiangxintuanheigu)rice varieties to uncover novel metabolites that enhance blast resistance.We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling,based on the infected status of M.oryzae in rice and the observed differences in shikimate accumulation between the two varieties.Following metabolomic analysis,we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control.Among these,apigenin-7-glucoside,rhamnetin,and spireoside were found to be effective in controlling blast disease,with spireoside demonstrating the most pronounced efficacy.We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M.oryzae,primarily through disrupting cell membrane integrity.However,spireoside did not induce rice immunity.Furthermore,spireoside was also effective in controlling sheath blight disease.Thus,spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases.展开更多
To address the problems of low accuracy by the CONWEP model and poor efficiency by the Coupled Eulerian-Lagrangian(CEL)method in predicting close-range air blast loads of cylindrical charges,a neural network-based sim...To address the problems of low accuracy by the CONWEP model and poor efficiency by the Coupled Eulerian-Lagrangian(CEL)method in predicting close-range air blast loads of cylindrical charges,a neural network-based simulation(NNS)method with higher accuracy and better efficiency was proposed.The NNS method consisted of three main steps.First,the parameters of blast loads,including the peak pressures and impulses of cylindrical charges with different aspect ratios(L/D)at different stand-off distances and incident angles were obtained by two-dimensional numerical simulations.Subsequently,incident shape factors of cylindrical charges with arbitrary aspect ratios were predicted by a neural network.Finally,reflected shape factors were derived and implemented into the subroutine of the ABAQUS code to modify the CONWEP model,including modifications of impulse and overpressure.The reliability of the proposed NNS method was verified by related experimental results.Remarkable accuracy improvement was acquired by the proposed NNS method compared with the unmodified CONWEP model.Moreover,huge efficiency superiority was obtained by the proposed NNS method compared with the CEL method.The proposed NNS method showed good accuracy when the scaled distance was greater than 0.2 m/kg^(1/3).It should be noted that there is no need to generate a new dataset again since the blast loads satisfy the similarity law,and the proposed NNS method can be directly used to simulate the blast loads generated by different cylindrical charges.The proposed NNS method with high efficiency and accuracy can be used as an effective method to analyze the dynamic response of structures under blast loads,and it has significant application prospects in designing protective structures.展开更多
基金financially supported by the National Natural Science Foundation of China(Nos.52204085 and 52104074)the Youth Science and Technology Foundation Key Laboratory for Mechanics in Fluid Solid Coupling System,Institute of Mechanics(No.E0XM040401)。
文摘This study primarily investigates the rock fracture mechanism of bottom cushion layer blasting and explores the effects of the bottom cushion layer on rock fragmentation.It involves analyses of the evolution patterns of blasting stress,characteristics of crack distribution,and rock fracture features in the specimens.First,blasting model experiments were carried out using the dynamic caustics principle to investigate the influence of bottom cushion layers and initiation methods on the integrity of the bottom rock mass.The experimental results indicate that the combined use of bottom cushion layers and inverse initiation effectively protects the integrity of the bottom rock mass.Subsequently,the process of stress wave propagation and dynamic crack propagation in rocks was simulated using the continuum-discontinuum element method(CDEM)and the Landau explosion source model,with varying thicknesses of bottom cushion layers.The numerical simulation results indicate that with increasing cushion thickness,the absorption of energy generated by the explosion becomes more pronounced,resulting in fewer cracks in the bottom rock mass.This illustrates the positive role of the cushion layer in protecting the integrity of the bottom rock mass.
基金financially supported by the National Natural Science Foundation of China (No. 52227805)the Fundamental Research Funds for the Central Universities (Ph.D. Top Innovative Talents Fund of China University of Mining and Technology Beijing) (No. BBJ2024085)the National Natural Science Foundation of China (Nos. 52474026 and 52208384)。
文摘The axial uncoupling coefficient and air deck effect in blasting significantly influence the effectiveness of rock fragmentation.This study employs a passive confinement device to conduct continuous charge and five different axial uncoupling coefficient blasting ex-periments on cylindrical iron ore samples to explain the rock-breaking mechanisms associated with various axial uncoupling coefficientsand air deck effects. It utilizes advanced techniques such as computer tomography(CT) scanning, deep learning, and three dimensional(3D) model reconstruction, to generate a 3D reconstruction model of “rock explosion cracks” under varying axial uncoupling coefficients.This model illustrates the spatial distribution and configurations of explosion cracks. Integrating box-counting dimension and fractal di-mension theories enables the quantitative analysis of the three-dimensional fracture field and the extent of damage in rocks subjected toexplosive forces. Laboratory 3D experimental results indicate that continuous charging produces the most extensive damage, while a un-coupling coefficient of 1.50(case 1) results in the least. A moderate air deck length enhances blasting effectiveness and rock fragmenta-tion. For identical charge quantities. In contrast, increasing the charge amount with a constant air deck length further augments rock frag-mentation. A rock blasting calculation model was developed using LS-DYNA numerical simulation software under various axial uncoup-ling coefficients. This model depicts the dynamic damage evolution characteristics of the rocks and variations in hole wall pressure. Thenumerical simulation results of cumulative rock damage align with the laboratory findings. In addition, increasing the air deck length re-duces the peak of the explosion shock wave, decreasing the peak pressure in the charge and air sections by 37.8% to 66.3%. These re-search outcomes provide valuable theoretical support for designing and optimizing axial uncoupling coefficients in practical applications.
基金This research was supported by the National Natural Science Foundation of China(Grant Nos.51974187)Intelligent Mine Blasting and Innovative Technology Platform Construction(LJ232410146045)Liaoning Revitalization Talents Program(XLYC2203173).
文摘Cross roadway collapses are a common occurrence in underground mining operations.While the influence of mining blasts on the stability of surrounding rock is acknowledged,the underlying mechanisms remain inadequately understood.This study investigates the characteristics and mechanisms of collapse in a shallow buried cross roadway subjected to mining blast disturbances,drawing insights from an engineering project in Anshan City,Northeast China.A strain-softening model based on unified strength theory was developed to effectively calculate and analyze the loosened zone thickness and surrounding rock displacement.The PFC3D-FLAC3D coupling method was employed to clarify the concentrated collapse area within the cross roadway,providing insight into the collapse mechanism through a cross-sectional model of the concentrated region.Results demonstrate that 50%of the cross roadway collapsed following the mining blast.Subsidence at the intersection was approximately one-fifth(0.66 m)of cross roadway’s net height,exceeding subsidence in other areas by 1.3.Under the action of repeated mining blasting,the cross section of the connection roadway forms a semi-elliptical high tensile stress zone.After the cumulative damage of the surrounding rock of the connection roadway exceeds the ultimate yield strength,the cumulative stress release causes the tensile failure of the surrounding rock.The plastic zone of the connecting roadway expands to three times of the initial,and continues to develop.The surrounding rock on both sides experienced tensile stress,cumulative stress release,and the vertical propagation of tensile cracks.
基金Supported by Project from National Natural Science Foundation of China(50674111)the National key Technology R&D Program in 10th Five Years Plan of China
文摘In the study of the application effectiveness of deep-hole controlled pre-splittingblasting technology,it was found through laboratory micro test and field study on a mine insouth China that under the technology,coal masses produce many irreversible cracks.Afterblasting,the nearer the distance from blasting hole,the larger the BET surface areaand volume ratio of the infiltration pore are;they increased by 11.47%and 5.73%,respectively.The coefficient of air permeability is increased 4 times.After 3 months,the gasdrainage rate was increased by 66%.In the first 15 days,the cumulative pumped gas was1.93 times of blasting before.The average absolute gas emission decreased by 63.46%.Experimental results show that deep-hole controlled pre-splitting blasting not only preventscoal and gas outburst,but also gives good economic results.
基金The project was supported by the National Science Foundation of China(41430640,U1704242).
文摘Coalbed gas extraction is an important means of exploiting and utilizing gas resources,as well as a means of preventing coal mine disasters.In view of the low gas extraction rate from coalbeds with high gas content and low permeability,a method of improving permeability through deep-hole cumulative blasting is applied to develop initial directional fractures using a jet flow.Under the action of the blasting stress wave and detonation gas wedge,the fractures extend over a large range within the coal,thereby improving coalbed permeability.This study focuses on the criteria of cumulative blasting-induced coalbed fracturing based on a literature review of the penetration effect of cumulative blasting.On this basis,we summarize the coal fracturing zone,crack extension process,and the key technologies of charging and hole sealing for cumulative blasting.In addition,the latest research progress in the optimization of field test drilling and blasting parameters for cumulative blasting is introduced.Research findings indicate that the permeability improvement mechanism of cumulative blasting could be further enhanced,and the technology and technical equipment are in urgent need of improvement.Finally,development trends in the cumulative blasting permeability improvement technique are identified.
基金supported by the National Natural Science Foundation of China (No.52204085)the Interdisciplinary Research Project for Young Teachers of USTB,Fundamental Research Funds for the Central Universities (No.FRF-IDRY-21-006).
文摘To study the effects of the initiation position on the damage and fracture characteristics of linear-charge blasting, blasting model experiments were conducted in this study using computed tomography scanning and three-dimensional reconstruction methods. The fractal damage theory was used to quantify the crack distribution and damage degree of sandstone specimens after blasting. The results showed that regardless of an inverse or top initiation, due to compression deformation and sliding frictional resistance, the plugging medium of the borehole is effective. The energy of the explosive gas near the top of the borehole is consumed. This affects the effective crushing of rocks near the top of the borehole, where the extent of damage to Sections Ⅰ and Ⅱ is less than that of Sections Ⅲ and Ⅳ. In addition, the analysis revealed that under conditions of top initiation, the reflected tensile damage of the rock at the free face of the top of the borehole and the compression deformation of the plug and friction consume more blasting energy, resulting in lower blasting energy efficiency for top initiation. As a result, the overall damage degree of the specimens in the top-initiation group was significantly smaller than that in the inverse-initiation group. Under conditions of inverse initiation, the blasting energy efficiency is greater, causing the specimen to experience greater damage. Therefore, in the engineering practice of rock tunnel cut blasting, to utilize blasting energy effectively and enhance the effects of rock fragmentation, using the inverse-initiation method is recommended. In addition, in three-dimensional(3D) rock blasting, the bottom of the borehole has obvious end effects under the conditions of inverse initiation, and the crack distribution at the bottom of the borehole is trumpet-shaped. The occurrence of an end effect in the 3D linear-charge blasting model experiment is related to the initiation position and the blocking condition.
基金This researchwas supported by the National Natural Science Foundation of China(No.52227805)the Fundamental Research Funds for Central Universities(No.2022JCCXLJ01).Awards were granted to the author Liyun Yang.
文摘Stress waves affect the stress field at the crack tip and dominate the dynamic crack propagation.Therefore,evaluating the influence of blasting stress waves on the crack propagation behavior and the mechanical characteristics of crack propagation is of great significance for engineering blasting.In this study,ANSYS/LS-DYNA was used for blasting numerical simulation,in which the propagation characteristics of blasting stress waves and stress field distribution at the crack tip were closely observed.Moreover,ABAQUS was applied for simulating the crack propagation path and calculating dynamic stress intensity factors(DSIFs).The universal function was calculated by the fractalmethod.The results show that:the compressive wave causes the crack to close and the reflected tensile wave drives the crack to initiate and propagate,and failure mode is mainly tensile failure.The crack propagation velocity varies with time,which increases at first and then decreases,and the crack arrest occurs due to the attenuation of stress waves and dissipation of the blasting energy.In addition,crack arrest toughness is smaller than the crack initiation toughness,applied pressure waveforms(such as the peak pressure,duration,waveforms,wavelengths and loading rates)have a great influence on DSIFs.It is conducive to our deep understanding or the study of blasting stress waves dominated fracture,suggesting a broad reference for the further development of rock blasting in engineering practice.
基金funding support from the National Natural Science Foundation of China(Grant Nos.52074298 and 52204164)Fundamental Research Funds for the Central Universities(Grant No.2022XJSB03).
文摘The deformation control of surrounding rock in gobside roadway with thick and hard roof poses a significant challenge to the safety and efficiency of coal mining.To address this issue,a novel approach combining directional and non-directional blasting techniques,known as combined blasting,was proposed.This study focuses on the experimental investigation of the proposed method in the 122108 working face in Caojiatan Coal Mine as the engineering background.The initial phase of the study involves physical model experiments to reveal the underlying mechanisms of combined blasting for protecting gob-side roadway with thick and hard roof.The results demonstrate that this approach effectively accelerates the collapse of thick and hard roofs,enhances the fragmentation and expansion coefficient of gangue,facilitates the filling of the goaf with gangue,and provides support to the overlying strata,thus reducing the subsidence of the overlying strata above the goaf.Additionally,the method involves cutting the main roof into shorter beams to decrease the stress and disrupt stress transmission pathways.Subsequent numerical simulations were conducted to corroborate the findings of the physical model experiments,thus validating the accuracy of the experimental results.Furthermore,field engineering experiments were performed,affirming the efficacy of the combined blasting method in mitigating the deformation of surrounding rock and achieving the desired protection of the gob-side roadway.
基金funded by the National Natural Science Foundation of China(No.42372331)the Henan Excellent Youth Science Fund Project(No.242300421145)the Colleges and Universities Youth and Innovation Science and Technology Support Plan of Shandong Province(No.2021KJ024).
文摘Water decoupling charge blasting excels in rock breaking,relying on its uniform pressure transmission and low energy dissipation.The water decoupling coefficients can adjust the contributions of the stress wave and quasi-static pressure.However,the quantitative relationship between the two contributions is unclear,and it is difficult to provide reasonable theoretical support for the design of water decoupling blasting.In this study,a theoretical model of blasting fracturing partitioning is established.The mechanical mechanism and determination method of the optimal decoupling coefficient are obtained.The reliability is verified through model experiments and a field test.The results show that with the increasing of decoupling coefficient,the rock breaking ability of blasting dynamic action decreases,while quasi-static action increases and then decreases.The ability of quasi-static action to wedge into cracks changes due to the spatial adjustment of the blast hole and crushed zone.The quasi-static action plays a leading role in the fracturing range,determining an optimal decoupling coefficient.The optimal water decoupling coefficient is not a fixed value,which can be obtained by the proposed theoretical model.Compared with the theoretical results,the maximum error in the model experiment results is 8.03%,and the error in the field test result is 3.04%.
文摘The data analysis of blasting sites has always been the research goal of relevant researchers.The rise of mobile blasting robots has aroused many researchers’interest in machine learning methods for target detection in the field of blasting.Serverless Computing can provide a variety of computing services for people without hardware foundations and rich software development experience,which has aroused people’s interest in how to use it in the field ofmachine learning.In this paper,we design a distributedmachine learning training application based on the AWS Lambda platform.Based on data parallelism,the data aggregation and training synchronization in Function as a Service(FaaS)are effectively realized.It also encrypts the data set,effectively reducing the risk of data leakage.We rent a cloud server and a Lambda,and then we conduct experiments to evaluate our applications.Our results indicate the effectiveness,rapidity,and economy of distributed training on FaaS.
基金Projects(52334003,52104111,52274249)supported by the National Natural Science Foundation of ChinaProject(2022YFC2903901)supported by the National Key R&D Project of ChinaProject(2024JJ4064)supported by the Natural Science Foundation of Hunan Province,China。
文摘Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.
文摘Blasting operations,which are crucial to open-pit mine production due to their simplicity and efficiency,require precise control through accurate vibration velocity calculations.The conventional Sadowski formula mainly focuses on blast center distance but neglects the amplification effect of blasting vibration waves by terraced terrain,from which the calculated blasting vibration velocities are smaller than the actual values,affecting the safety of the project.To address this issue,our model introduces the influences of slope and time into Sadowski formula to measure safety through blast vibration displacement.In the northern section of the open-pit quartz mine in Jinchang City,Gansu Province,China,the data of a continuous blasting slope project are referred to.Our findings reveal a noticeable vibration amplification effect during blasting when a multi-stage slope platform undergoes a sudden cross-sectional change near the upper overhanging surface.The amplification vibration coefficient increases with height,while vibration waves within rocks decrease from bottom to top.Conversely,platforms without distinct crosssectional changes exhibit no pronounced amplification during blasting.In addition,the vibration intensity decreases with distance as the rock height difference change propagates.The results obtained by the proposed blast vibration displacement equation incorporating slope shape influence closely agree with real-world scenarios.According to Pearson correlation coefficient(PPMCC)analysis,the average accuracy rate of our model is 88.84%,which exceeds the conventional Sadowski formula(46.92%).
基金financially supported by the NationalNatural Science Foundation of China(Grant No.42072309)the Fundamental Research Funds for National University,China University of Geosciences(Wuhan)(Grant No.CUGDCJJ202217)+1 种基金the Knowledge Innovation Program of Wuhan-Basic Research(Grant No.2022020801010199)the Hubei Key Laboratory of Blasting Engineering Foundation(HKLBEF202002).
文摘Accurately estimating blasting vibration during rock blasting is the foundation of blasting vibration management.In this study,Tuna Swarm Optimization(TSO),Whale Optimization Algorithm(WOA),and Cuckoo Search(CS)were used to optimize two hyperparameters in support vector regression(SVR).Based on these methods,three hybrid models to predict peak particle velocity(PPV)for bench blasting were developed.Eighty-eight samples were collected to establish the PPV database,eight initial blasting parameters were chosen as input parameters for the predictionmodel,and the PPV was the output parameter.As predictive performance evaluation indicators,the coefficient of determination(R2),rootmean square error(RMSE),mean absolute error(MAE),and a10-index were selected.The normalizedmutual information value is then used to evaluate the impact of various input parameters on the PPV prediction outcomes.According to the research findings,TSO,WOA,and CS can all enhance the predictive performance of the SVR model.The TSO-SVR model provides the most accurate predictions.The performances of the optimized hybrid SVR models are superior to the unoptimized traditional prediction model.The maximum charge per delay impacts the PPV prediction value the most.
文摘This study utilizes empirical equations to describe the propagation of vibrations induced by blasting, with the goal of predicting the attenuation of Peak Particle Velocity (PPV) at the Yaramoko mine in Bagassi, Burkina Faso, a site characterized by granitoid rock. Four empirical PPV prediction equations were employed, so-called Duvall & Fogelson (or the United States Bureau of Mines “USBM”), Langefors and Kihlstrom, Ambressys-Hendron, and the Bureau of Indian Standard. The constant parameters for each of these equations, referred to as site constants, were derived from linear regression curves. The results show that the site constants k, a, and b of 4762, 0.869, and 1.737, respectively, derived from the general prediction equation by Davies, PPV = kQaD−b, based on Duvall & Fogelson, are in good agreement with values of 4690, 0.9, and 1.69, respectively, for similar rock types in Spain. Regarding the impacts of blasting on houses, the findings indicate that houses built from laterite-block bricks in the village of Bagassi are the most vulnerable to vibration waves, followed by those constructed with cinder-block bricks. In contrast, houses made of banco bricks are the most resilient. Additionally, it was determined that during blasting operations, adjusting the blasting parameters to ensure the PPV does not exceed 2 mm/s at the level of nearby dwellings can minimize the appearance of cracks in houses.
基金supported by the National Key R&D Program of China (Grant No.2020YFA0711802).
文摘Integrating liquid CO_(2)phase transition blasting(LCPTB)technology with hydraulic fracturing(HF)methods can help reduce wellbore damage,create multiple radial fractures,and establish a complex fracture network.This approach significantly increases the recovery efficiency of low-permeability oil and gas fields.Accurately calculating the number of fractures caused by LCPTB is necessary to predict production enhancement effects and optimize subsequent HF designs.However,few studies are reported on large-scale physical model experiments in terms of a method for calculating the fracture number.This study analyzed the initiation and propagation of cracks under LCPTB,derived a calculation formula for crack propagation radius under stress waves,and then proposed a new,fast,and accurate method for calculating the fracture number using the principle of mass conservation.Through ten rock-breaking tests using LCPTB,the study confirmed the effectiveness of the proposed calculation approach and elucidated the variation rule of explosion pressure,rock-breaking scenario,and the impact of varying parameters on fracture number.The results show that the new calculation method is suitable for fracturing technologies with high pressure rates.Recommendations include enlarging the diameter of the fracturing tube and increasing the liquid CO_(2) mass in the tube to enhance fracture effectiveness.Moreover,the method can be applied to other fracturing technologies,such as explosive fracturing(EF)within HF formations,indicating its broader applicability and potential impact on optimizing unconventional resource extraction technologies.
基金Project(2021JJ10063)supported by the Natural Science Foundation of Hunan Province,ChinaProject(202115)supported by the Science and Technology Progress and Innovation Project of Hunan Provincial Department of Transportation,ChinaProject(2021K094-Z)supported by the Science and Technology Research and Development Program of China Railway Guangzhou Group Co.,Ltd。
文摘Blasting-induced cracks in the rock surrounding deeply buried tunnels can result in water gushing and rock mass collapse,posing significant safety risks.However,previous theoretical studies on the range of blasting-induced cracks often ignore the impact of the in-situ stress,especially that of the intermediate principal stress.The particle displacement−crack radius relationship was established in this paper by utilizing the blasthole cavity expansion equation,and theoretical analytical formulas of the stress−displacement relationship and the crack radius were derived with unified strength theory to accurately assess the range of cracks in deep surrounding rock under a blasting load.Parameter analysis showed that the crushing zone size was positively correlated with in-situ stress,intermediate principal stress,and detonation pressure,whereas negatively correlated with Poisson ratio and decoupling coefficient.The dilatancy angle-crushing zone size relationship exhibited nonmonotonic behavior.The relationships in the crushing zone and the fracture zone exhibited opposite trends under the influence of only in-situ stress or intermediate principal stress.As the in-situ stress increased from 0 to 70 MPa,the rate of change in the crack range and the attenuation rate of the peak vibration velocity gradually slowed.
基金financially supported by National Natural Science Foundation of China(Grant.22076189)National Key Research and Development Program of China(No.2023YFC3707003)the Joint Fund of Yulin University and Dalian National Laboratory for Clean Energy(Grant.YLU-DNL Fund 2022003).
文摘Blast furnace gas(BFG)is an important by-product energy for the iron and steel industry and has been widely used for heating or electricity generation.However,the undesirable contaminants in BFG(especially H_(2)S)generate harmful environmental emissions.The desulfurization of BFG is urgent for integrated steel plants due to the stringent ultra-low emission standards.Compared with other desulfurization materials,zeolite-based adsorbents represent a viable option with low costs and long service life.In this study,an ammonia-induced CuO modified 13X adsorbent(NH_(3)–CuO/13X)was prepared for H_(2)S removal from simulated BFG at low temperature.The XRD,H_(2)-TPR and TEM analysis proved that smaller CuO particles were formed and the dispersion of Cu on the surface of 13X zeolite was improved via the induction of ammonia.Evaluation on H_(2)S adsorption performance of the adsorbent was carried out using simulated BFG,and the results showed that NH_(3)–CuO/13X-3 has better breakthrough sulfur capacity,which was more than twice the sulfur capacity of CuO/13X.It is proposed that the enhanced desulfurization performance of NH_(3)–CuO/13X is attributed to an abundant pore of 13X,and combined action of 13X and CuO.This work provided an effective way to improve the sulfur capacity of zeolite-based adsorbents via impregnation method by ammonia induction.
基金funded by grants from the National Key R&D Project(2023YFD1400201-02,2023YFD1400203-02)the National Natural Science Foundation of China(31870137)+1 种基金the National Transgenic Research Project(2015ZX08001-002)the Key R&D Project of Guangdong Province(2022B0202060005).
文摘Asian rice comprises two major subspecies:Xian(X)and Geng(G),and the diverged resistance genes(R)have provided a foundation for breeding improved cultivars to control rice blast disease.After conducting two-phase allele mining using six updated FNP marker systems,the functional haplotypes at Pit,Pib,and Pi63 strictly diverged into the X-populations and were defined as X-R loci,while those at Pi54,Pi37,and Pi36 into the G-populations as G-R loci.The genic diversity at the three X-R loci(16 alleles)was twofold higher than that at the three G-R loci(8 alleles),and the allelic diversity in the Southern region(21 alleles)was nearly double that in the Northeastern region(11 alleles).Both observations reflect a significant difference in genetic diversity between X-and G-populations,and indicate that the effective R-genes mainly originated from X-subspecies.Based on the allelic structures characterized by a set of 10 parameters,8 and 16 alleles were respectively recognized as favorable and promising ones for the regional breeding programs.The genotypic structures of the two regional populations were almost different,indicating that the diverged alleles have been further assembled into two series of regional genotypes through long-term breeding programs,despite the presence of one-third of region-common alleles.The genotypic diversity in the Southern region(55 genotypes)was nearly twice as high as that in the Northeastern region(28),which perfectly reflects the aforementioned differences in both genic and allelic diversities.After analyzing the genotypic structures using a set of 13 parameters,4 and 23 genotypes,respectively,can be recommended as the favorable and promising ones for the regional breeding programs.The case study serves as a concrete sample of how to identify the favorable and promising alleles and genotypes,and beneficial parents based their comprehensive population structures for gene-designed breeding.
基金supported by the National Natural Science Foundation of China(Grant Nos.32172419,32372555,32121003,32425005,32072407,and 32272033)the National Key Research and Development Program of China(Grant No.2021YFA1300702)the Sichuan Science and Technology Program,China(Grant Nos.2023NSFSC0005,2023NSFSC1996,2024NSFSC0322,2024YFNH0014,2022NSFSC1755,2022-NSFSC1650,2022NSFSC0156,and 2022NSFSC0166).
文摘The application of fungicides is an effective strategy for controlling plant diseases.Among these agents,plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature.Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens.However,only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites.In this study,we conducted a metabolomic analysis on both blast-resistant(Digu)and-susceptible(Lijiangxintuanheigu)rice varieties to uncover novel metabolites that enhance blast resistance.We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling,based on the infected status of M.oryzae in rice and the observed differences in shikimate accumulation between the two varieties.Following metabolomic analysis,we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control.Among these,apigenin-7-glucoside,rhamnetin,and spireoside were found to be effective in controlling blast disease,with spireoside demonstrating the most pronounced efficacy.We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M.oryzae,primarily through disrupting cell membrane integrity.However,spireoside did not induce rice immunity.Furthermore,spireoside was also effective in controlling sheath blight disease.Thus,spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases.
基金financially supported by the National Natural Science Foundation of China(Grant Nos.52271317 and 52071149)the Fundamental Research Funds for the Central Universities(HUST:2019kfy XJJS007)。
文摘To address the problems of low accuracy by the CONWEP model and poor efficiency by the Coupled Eulerian-Lagrangian(CEL)method in predicting close-range air blast loads of cylindrical charges,a neural network-based simulation(NNS)method with higher accuracy and better efficiency was proposed.The NNS method consisted of three main steps.First,the parameters of blast loads,including the peak pressures and impulses of cylindrical charges with different aspect ratios(L/D)at different stand-off distances and incident angles were obtained by two-dimensional numerical simulations.Subsequently,incident shape factors of cylindrical charges with arbitrary aspect ratios were predicted by a neural network.Finally,reflected shape factors were derived and implemented into the subroutine of the ABAQUS code to modify the CONWEP model,including modifications of impulse and overpressure.The reliability of the proposed NNS method was verified by related experimental results.Remarkable accuracy improvement was acquired by the proposed NNS method compared with the unmodified CONWEP model.Moreover,huge efficiency superiority was obtained by the proposed NNS method compared with the CEL method.The proposed NNS method showed good accuracy when the scaled distance was greater than 0.2 m/kg^(1/3).It should be noted that there is no need to generate a new dataset again since the blast loads satisfy the similarity law,and the proposed NNS method can be directly used to simulate the blast loads generated by different cylindrical charges.The proposed NNS method with high efficiency and accuracy can be used as an effective method to analyze the dynamic response of structures under blast loads,and it has significant application prospects in designing protective structures.