The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievem...The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved （including an estimate of the number of leucocyte surface molecules still to be discovered）, and how the field can best move forward.展开更多
Objective:Vitiligo is a relatively common skin disfiguring disorder that exhibits a fluctuating course between activity and stability,making monitoring and management challenging.Autoimmunity plays a crucial role in t...Objective:Vitiligo is a relatively common skin disfiguring disorder that exhibits a fluctuating course between activity and stability,making monitoring and management challenging.Autoimmunity plays a crucial role in the pathogenesis of vitiligo.Numerous autoimmune disorders have been associated with both CD27 and chemokine(C-X-C motif)ligand 10(CXCL10).However,trials evaluating their role in vitiligo are lacking in the Egyptian setting.We evaluated the circulating levels of these 2 biomarkers in patients with vitiligo and the possible correlation between their levels and disease activity.Methods:This cross-sectional study included 70 patients with vitiligo and 20 healthy controls.The patients were clinically assessed and then divided into active and stable groups according to clinical signs of activity and Vitiligo Disease Activity scores.The levels of CD27 and CXCL10 in the serum were assessed using an enzyme-linked immunosorbent assay in both the patients and the controls,then the Mann-Whitney U and Kruskal-Wallis tests were used to analyze the difference between the groups.Results:Active and stable vitiligo patients have significantly higher median serum CXCL10(385.9 and 245.2 pg/mL)and CD27(61.6 and 66.5 ng/mL)levels compared to the controls(193 pg/mL and 52.5 ng/mL,respectively,all P<0.05).In vitiligo cases,although CXCL10 levels significantly increased with disease activity(P<0.001),CD27 levels were comparable between the 2 subgroups(P=0.953).CXCL10 positively correlated with disease activity(r=0.887,P<0.001).CXCL10 had a higher sensitivity and a lower specificity(95.7%and 60.0%,respectively)compared to CD27(71.4%and 75%,respectively)for differentiating cases from controls.Conclusion:There is a possibility that CXCL10 and CD27 are involved in the development and course of vitiligo.展开更多
目的比较炎症性肠病患者肠黏膜炎症组织、非炎症组织及正常对照者肠黏膜CD27激活表达的差异,探讨CD27激活表达在炎症性肠病发病中的意义。方法共纳入32例克罗恩病患者、41例溃疡性结肠炎患者及40例正常对照者。分别应用West-ern blot试...目的比较炎症性肠病患者肠黏膜炎症组织、非炎症组织及正常对照者肠黏膜CD27激活表达的差异,探讨CD27激活表达在炎症性肠病发病中的意义。方法共纳入32例克罗恩病患者、41例溃疡性结肠炎患者及40例正常对照者。分别应用West-ern blot试验和SYBR-green real time PCR方法分析炎症性肠病患者肠黏膜炎症组织、非炎症组织及正常对照者肠黏膜CD27蛋白及其mRNA的表达。数据处理使用GraphPad Prism 5软件。结果克罗恩病和溃疡性结肠炎患者肠黏膜炎症组织CD27蛋白及其mRNA表达均显著高于非炎症组织及正常对照组织(P均<0.01);克罗恩病患者肠黏膜非炎症组织CD27蛋白及其mRNA表达显著高于正常对照组织(P=0.000);溃疡性结肠炎患者肠黏膜非炎症组织CD27蛋白表达显著高于正常对照组织(P=0.000)。结论炎症性肠病患者肠黏膜组织中存在CD27的激活表达,这种激活效应不仅出现在内镜表现为炎症性肠病的炎症组织中,甚至出现在炎症性肠病患者内镜表现为正常的肠黏膜中,CD27的激活表达是炎症性肠病发病的早期事件。展开更多
文摘The 8^th International Workshop on Human Leucocyte Differentiation Antigens （chaired by HZ and managed by BS） was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved （including an estimate of the number of leucocyte surface molecules still to be discovered）, and how the field can best move forward.
文摘Objective:Vitiligo is a relatively common skin disfiguring disorder that exhibits a fluctuating course between activity and stability,making monitoring and management challenging.Autoimmunity plays a crucial role in the pathogenesis of vitiligo.Numerous autoimmune disorders have been associated with both CD27 and chemokine(C-X-C motif)ligand 10(CXCL10).However,trials evaluating their role in vitiligo are lacking in the Egyptian setting.We evaluated the circulating levels of these 2 biomarkers in patients with vitiligo and the possible correlation between their levels and disease activity.Methods:This cross-sectional study included 70 patients with vitiligo and 20 healthy controls.The patients were clinically assessed and then divided into active and stable groups according to clinical signs of activity and Vitiligo Disease Activity scores.The levels of CD27 and CXCL10 in the serum were assessed using an enzyme-linked immunosorbent assay in both the patients and the controls,then the Mann-Whitney U and Kruskal-Wallis tests were used to analyze the difference between the groups.Results:Active and stable vitiligo patients have significantly higher median serum CXCL10(385.9 and 245.2 pg/mL)and CD27(61.6 and 66.5 ng/mL)levels compared to the controls(193 pg/mL and 52.5 ng/mL,respectively,all P<0.05).In vitiligo cases,although CXCL10 levels significantly increased with disease activity(P<0.001),CD27 levels were comparable between the 2 subgroups(P=0.953).CXCL10 positively correlated with disease activity(r=0.887,P<0.001).CXCL10 had a higher sensitivity and a lower specificity(95.7%and 60.0%,respectively)compared to CD27(71.4%and 75%,respectively)for differentiating cases from controls.Conclusion:There is a possibility that CXCL10 and CD27 are involved in the development and course of vitiligo.
文摘目的比较炎症性肠病患者肠黏膜炎症组织、非炎症组织及正常对照者肠黏膜CD27激活表达的差异,探讨CD27激活表达在炎症性肠病发病中的意义。方法共纳入32例克罗恩病患者、41例溃疡性结肠炎患者及40例正常对照者。分别应用West-ern blot试验和SYBR-green real time PCR方法分析炎症性肠病患者肠黏膜炎症组织、非炎症组织及正常对照者肠黏膜CD27蛋白及其mRNA的表达。数据处理使用GraphPad Prism 5软件。结果克罗恩病和溃疡性结肠炎患者肠黏膜炎症组织CD27蛋白及其mRNA表达均显著高于非炎症组织及正常对照组织(P均<0.01);克罗恩病患者肠黏膜非炎症组织CD27蛋白及其mRNA表达显著高于正常对照组织(P=0.000);溃疡性结肠炎患者肠黏膜非炎症组织CD27蛋白表达显著高于正常对照组织(P=0.000)。结论炎症性肠病患者肠黏膜组织中存在CD27的激活表达,这种激活效应不仅出现在内镜表现为炎症性肠病的炎症组织中,甚至出现在炎症性肠病患者内镜表现为正常的肠黏膜中,CD27的激活表达是炎症性肠病发病的早期事件。
