Two series of aromatic-aliphatic random copolyesters(PEBTOXS)with diverse diol ratios have been synthesized by direct melting polycondensation.Two kinds of diols(glycol(EG)and 1,4-butanediol(BD))are used in combinatio...Two series of aromatic-aliphatic random copolyesters(PEBTOXS)with diverse diol ratios have been synthesized by direct melting polycondensation.Two kinds of diols(glycol(EG)and 1,4-butanediol(BD))are used in combination in order to adjust crystallization and tensile properties,and three kinds of diacids(dimethyl terephthalate(DMT),diethyl oxalate(DEOX),and sebacic acid(SA))are involved.~1H-NMR spectra quantify both composition and structure,and show that the final contents in copolyesters appropriate to the ...展开更多
In the study of motif discovery, especially the transcription factor DNA binding sites discovery, a too long input sequence would return non-informative motifs rather than those biological functional motifs. This pape...In the study of motif discovery, especially the transcription factor DNA binding sites discovery, a too long input sequence would return non-informative motifs rather than those biological functional motifs. This paper gave theoretical analyses and computational experiments to suggest the length limits of the input sequence. When the sequence length exceeds a certain critical point, the probability of discovering the motif decreases sharply. The work not only gave an explanation on the unsatisfying results of the existed motif discovery problems that the input sequence length might be too long and exceed the point, but also provided an estimation of input sequence length we should accept to get more meaningful and reliable results in motif discovery.展开更多
As an essential function of encrypted Internet traffic analysis,encrypted traffic service classification can support both coarse-grained network service traffic management and security supervision.However,the traditio...As an essential function of encrypted Internet traffic analysis,encrypted traffic service classification can support both coarse-grained network service traffic management and security supervision.However,the traditional plaintext-based Deep Packet Inspection(DPI)method cannot be applied to such a classification.Moreover,machine learning-based existing methods encounter two problems during feature selection:complex feature overcost processing and Transport Layer Security(TLS)version discrepancy.In this paper,we consider differences between encryption network protocol stacks and propose a composite deep learning-based method in multiprotocol environments using a sliding multiple Protocol Data Unit(multiPDU)length sequence as features by fully utilizing the Markov property in a multiPDU length sequence and maintaining suitability with a TLS-1.3 environment.Control experiments show that both Length-Sensitive(LS)composite deep learning model using a capsule neural network and LS-long short time memory achieve satisfactory effectiveness in F1-score and performance.Owing to faster feature extraction,our method is suitable for actual network environments and superior to state-of-the-art methods.展开更多
In order to understand molecular characterization of Citrus tatter leaf virus（CTLV） isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced...In order to understand molecular characterization of Citrus tatter leaf virus（CTLV） isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR.The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0%and 78.8-98.0%nucleotide sequence identity,respectively,with other Apple stem grooving virus（ASGV） or CTLV strains available in GenBank.Unexpectedly,CTLV-MTH showed the highest nucleotide sequence identity（91%） with an apple isolate of ASGV,followed by 86.5%with ASGV-HH and 85.7%with ASGV-CHN.Furthermore,CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree,suggesting it has a closer relationship to ASGV than to CTLV.Therefore,it can be concluded roughly that CTLV may be not a distinct strains of ASGV.We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.展开更多
A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using th...A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using the mapping approach of bulked extremes and recessive class, Wbph6(t) was mapped onto the short arm of chromosome 11 with a genetic distance of 21.2 cM to SSLP marker RM167.展开更多
A series ofbutadiene-isoprene copolymers （BIR） with various compositions were synthesized with a neodymiumbased catalyst system. The microstructure, composition and sequence of copolymers were characterized by FTIR ...A series ofbutadiene-isoprene copolymers （BIR） with various compositions were synthesized with a neodymiumbased catalyst system. The microstructure, composition and sequence of copolymers were characterized by FTIR and ^13C-NMR spectroscopy. The crystallization behavior of the BIR copolymers was investigated by DSC analysis. The results demonstrate that the content of cis-1,4 configuration in both butadiene （Bd） and isoprene （Ip） units are around 98% when Bd content in feed （fBd） covering the range from 55.7 mol% to 96.0 mol%. The reactivity ratios of Bd and Ip were determined to be 1.40 and 0.48 respectively. The random copolymers of Bd and Ip show only one glass transition temperature （Tg） from -107.4℃ to -80.5℃, which is dependent on the composition and fits nicely with Fox equation. The sequence distribution followed the first-order Markov statistical model. It is found that the copolymer chains with higher Bd content contain longer polybutadiene （PBd） segments, and the sequence length of PBd segments （NBd） exhibits great influence on the crystallization behavior of the copolymer. The copolymers with NBd ≥ 11.8 could crystallize at low temperatures （-71℃ to -43℃）. The crystallization temperature and enthalpy values decreased gradually with decreasing NBd. The copolymers with NBd ≤ 7.9 are amorphous even at very low temperatures （0℃ to -150℃） due to the short PBd segments.展开更多
A series of the copolymers of ethylene with 1-hexene （M1-M9） synthesized by metallocene catalyst Et[Ind]2ZrC1JMAO was studied by differential scanning calorimetry and successive self-nucleation and annealing （SSA）...A series of the copolymers of ethylene with 1-hexene （M1-M9） synthesized by metallocene catalyst Et[Ind]2ZrC1JMAO was studied by differential scanning calorimetry and successive self-nucleation and annealing （SSA） thermal fractionation. The distribution of methylene sequence length （MSL） in the different copolymers was determined using the SSA method. The comonomer contents of samples M4 and M5 are 2.04 mol% and 2.78 mol%, respectively. Both M4 and M5 have low comonomer content and their MSL distribution profiles exhibit a monotonous increase trend with their MSL. The longest MSL of M5 is 167, and its corresponding molar percent is 43.95%, which is higher than that of M4. Moreover, the melting temperature （Tm） of M5 is also higher than that of M4. The comonomer contents of samples M7, M8, and M9 are 8.73 mol%, 14.18 mol% and 15.05 mol%, respectively. M7, M8, and M9 have high comonomer contents, and their MSL distribution profiles display unimodality. M7 has a lower peak value of 33 and a narrow MSL distribution, resulting in a Tm lower than that of M8 and M9. The MSL and its distribution are also key points that influence the melting behavior of copolymers. Sometimes, MSL and its distribution of copolymers have a greater impact on it than the total comonomer contents, which is different from traditional views.展开更多
The stereo-defects distribution of polypropylene of the two industry biaxially oriented polypropylene （BOPP） samples T28FE and F28SO with different processing properties was studied through successive self-nucleatio...The stereo-defects distribution of polypropylene of the two industry biaxially oriented polypropylene （BOPP） samples T28FE and F28SO with different processing properties was studied through successive self-nucleation and annealing （SSA） technique. It was found that there were more medium isotactic components in sample F28SO, and the isotactic sequence length of polypropylene of sample F28SO was shorter and the isotactic sequence length distribution of polypropylene of sample F28SO was broader, which could be processed well at high-speed orientation during the processing of BOPP films. This result indicates that the isotactic sequence length distribution of polypropylene is related to the processing speed during preparing BOPP films, and the stereo-defects distribution of polypropylene has an important influence on its processing ability.展开更多
In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy （EIS）. Using the least flow resistance path principle, the hyd...In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy （EIS）. Using the least flow resistance path principle, the hydrodynamic cell trapping in serpentine arrays can be carried out in a deterministic and automatic manner without the assistance of any external fields. The experimental results show that a cell trap rate of higher than 95% can be easily achieved in our ceil trapping microdevices. Using the maximum length sequences （MLS） technique, our home-made EIS is capable of measuring the impedance spectrum ranging from 1.953 kHz to 1 MHz in approximately 0.5 ms. Finally, on the basis of the developed single-cell analysis system, we precisely monitor the trapping process of human breast tumor cells （MCF-7 cells） according to the changes of electrical impedance. The MCF-7 cells with different trapping conditions or sizes can also be clearly distinguished through the impedance signals. Our portable single-cell analysis system may provide a promising tool to monitor single cells for long periods of time or to discriminate cell types.展开更多
基金This work was supported by China Postdoctoral Foundation(No.2003034321)Young Teacher Foundation of Tianjin University.
文摘Two series of aromatic-aliphatic random copolyesters(PEBTOXS)with diverse diol ratios have been synthesized by direct melting polycondensation.Two kinds of diols(glycol(EG)and 1,4-butanediol(BD))are used in combination in order to adjust crystallization and tensile properties,and three kinds of diacids(dimethyl terephthalate(DMT),diethyl oxalate(DEOX),and sebacic acid(SA))are involved.~1H-NMR spectra quantify both composition and structure,and show that the final contents in copolyesters appropriate to the ...
文摘In the study of motif discovery, especially the transcription factor DNA binding sites discovery, a too long input sequence would return non-informative motifs rather than those biological functional motifs. This paper gave theoretical analyses and computational experiments to suggest the length limits of the input sequence. When the sequence length exceeds a certain critical point, the probability of discovering the motif decreases sharply. The work not only gave an explanation on the unsatisfying results of the existed motif discovery problems that the input sequence length might be too long and exceed the point, but also provided an estimation of input sequence length we should accept to get more meaningful and reliable results in motif discovery.
基金supported by the General Program of the National Natural Science Foundation of China under Grant No.62172093the National Key R&D Program of China under Grant No.2018YFB1800602+1 种基金2019 Industrial Internet Innovation and Development Project,Ministry of Industry and Information Technology(MIIT)under Grant No.6709010003Ministry of Education-China Mobile Research Fund under Grant No.MCM20180506。
文摘As an essential function of encrypted Internet traffic analysis,encrypted traffic service classification can support both coarse-grained network service traffic management and security supervision.However,the traditional plaintext-based Deep Packet Inspection(DPI)method cannot be applied to such a classification.Moreover,machine learning-based existing methods encounter two problems during feature selection:complex feature overcost processing and Transport Layer Security(TLS)version discrepancy.In this paper,we consider differences between encryption network protocol stacks and propose a composite deep learning-based method in multiprotocol environments using a sliding multiple Protocol Data Unit(multiPDU)length sequence as features by fully utilizing the Markov property in a multiPDU length sequence and maintaining suitability with a TLS-1.3 environment.Control experiments show that both Length-Sensitive(LS)composite deep learning model using a capsule neural network and LS-long short time memory achieve satisfactory effectiveness in F1-score and performance.Owing to faster feature extraction,our method is suitable for actual network environments and superior to state-of-the-art methods.
基金supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(201203076)the 111 Project,China(B12006)+1 种基金the Fundamental Research Funds for the Central Universities,China(XDJK2013C161,SWU113097)the Basic and Frontier Research Programs of Chongqing,China(CSTC2014jcyjA80033)
文摘In order to understand molecular characterization of Citrus tatter leaf virus（CTLV） isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR.The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0%and 78.8-98.0%nucleotide sequence identity,respectively,with other Apple stem grooving virus（ASGV） or CTLV strains available in GenBank.Unexpectedly,CTLV-MTH showed the highest nucleotide sequence identity（91%） with an apple isolate of ASGV,followed by 86.5%with ASGV-HH and 85.7%with ASGV-CHN.Furthermore,CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree,suggesting it has a closer relationship to ASGV than to CTLV.Therefore,it can be concluded roughly that CTLV may be not a distinct strains of ASGV.We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.
文摘A rice population consisting of 90 TN1/Guiyigu F3 lines was employed to analyze the linkage between DNA markers and a new gene Wbph6(t) conferring resistance to whitebacked planthopper, Sogatella furcifera By using the mapping approach of bulked extremes and recessive class, Wbph6(t) was mapped onto the short arm of chromosome 11 with a genetic distance of 21.2 cM to SSLP marker RM167.
基金supported by the National 863 program(No.2006AA03Z552)China Petroleum Chemical Corp. (SINOPEC) and Program for Changjiang Scholars and Innovative Research Teams in Universities(IRT0706)
文摘A series ofbutadiene-isoprene copolymers （BIR） with various compositions were synthesized with a neodymiumbased catalyst system. The microstructure, composition and sequence of copolymers were characterized by FTIR and ^13C-NMR spectroscopy. The crystallization behavior of the BIR copolymers was investigated by DSC analysis. The results demonstrate that the content of cis-1,4 configuration in both butadiene （Bd） and isoprene （Ip） units are around 98% when Bd content in feed （fBd） covering the range from 55.7 mol% to 96.0 mol%. The reactivity ratios of Bd and Ip were determined to be 1.40 and 0.48 respectively. The random copolymers of Bd and Ip show only one glass transition temperature （Tg） from -107.4℃ to -80.5℃, which is dependent on the composition and fits nicely with Fox equation. The sequence distribution followed the first-order Markov statistical model. It is found that the copolymer chains with higher Bd content contain longer polybutadiene （PBd） segments, and the sequence length of PBd segments （NBd） exhibits great influence on the crystallization behavior of the copolymer. The copolymers with NBd ≥ 11.8 could crystallize at low temperatures （-71℃ to -43℃）. The crystallization temperature and enthalpy values decreased gradually with decreasing NBd. The copolymers with NBd ≤ 7.9 are amorphous even at very low temperatures （0℃ to -150℃） due to the short PBd segments.
基金financially supported by the National Natural Science Foundation of China(Nos.20734006 and 50921062)
文摘A series of the copolymers of ethylene with 1-hexene （M1-M9） synthesized by metallocene catalyst Et[Ind]2ZrC1JMAO was studied by differential scanning calorimetry and successive self-nucleation and annealing （SSA） thermal fractionation. The distribution of methylene sequence length （MSL） in the different copolymers was determined using the SSA method. The comonomer contents of samples M4 and M5 are 2.04 mol% and 2.78 mol%, respectively. Both M4 and M5 have low comonomer content and their MSL distribution profiles exhibit a monotonous increase trend with their MSL. The longest MSL of M5 is 167, and its corresponding molar percent is 43.95%, which is higher than that of M4. Moreover, the melting temperature （Tm） of M5 is also higher than that of M4. The comonomer contents of samples M7, M8, and M9 are 8.73 mol%, 14.18 mol% and 15.05 mol%, respectively. M7, M8, and M9 have high comonomer contents, and their MSL distribution profiles display unimodality. M7 has a lower peak value of 33 and a narrow MSL distribution, resulting in a Tm lower than that of M8 and M9. The MSL and its distribution are also key points that influence the melting behavior of copolymers. Sometimes, MSL and its distribution of copolymers have a greater impact on it than the total comonomer contents, which is different from traditional views.
基金financially supported by the National Natural Science Foundation of China(No.51073170)
文摘The stereo-defects distribution of polypropylene of the two industry biaxially oriented polypropylene （BOPP） samples T28FE and F28SO with different processing properties was studied through successive self-nucleation and annealing （SSA） technique. It was found that there were more medium isotactic components in sample F28SO, and the isotactic sequence length of polypropylene of sample F28SO was shorter and the isotactic sequence length distribution of polypropylene of sample F28SO was broader, which could be processed well at high-speed orientation during the processing of BOPP films. This result indicates that the isotactic sequence length distribution of polypropylene is related to the processing speed during preparing BOPP films, and the stereo-defects distribution of polypropylene has an important influence on its processing ability.
基金supported by the National Natural Science Foundation of China(Grant Nos.51505082,51775111,51375089 and 81572906)the Natural Science Foundation of Jiangsu Province(Grant No.BK20150606)+3 种基金the"333"Project of Jiangsu Province(Grant No.BRA2015291)the Jiangsu Graduate Innovative Research Program(Grant No.KYLX_0098)the Scientific Research Foundation of Graduate School of Southeast University(Grant No.YBJJ1428)the Open Foundation of the State Key Laboratory of Fluid Power and Mechatronic Systems(Grant No.GZKF-201501)
文摘In this paper, we present a portable single-cell analysis system with the hydrodynamic cell trapping and the broadband electrical impedance spectroscopy （EIS）. Using the least flow resistance path principle, the hydrodynamic cell trapping in serpentine arrays can be carried out in a deterministic and automatic manner without the assistance of any external fields. The experimental results show that a cell trap rate of higher than 95% can be easily achieved in our ceil trapping microdevices. Using the maximum length sequences （MLS） technique, our home-made EIS is capable of measuring the impedance spectrum ranging from 1.953 kHz to 1 MHz in approximately 0.5 ms. Finally, on the basis of the developed single-cell analysis system, we precisely monitor the trapping process of human breast tumor cells （MCF-7 cells） according to the changes of electrical impedance. The MCF-7 cells with different trapping conditions or sizes can also be clearly distinguished through the impedance signals. Our portable single-cell analysis system may provide a promising tool to monitor single cells for long periods of time or to discriminate cell types.
