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Larvicidal and antibiofilm potential of three mountain plants:Centaurea ensiformis,Origanum hypericifolium,Paeonia turcica
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作者 Fatma Bursali Rukiye Yavaser Boncooğlu +2 位作者 Mustapha Touray Mehmet Aytar Ali Celik 《Traditional Medicine Research》 2025年第4期1-10,共10页
Background:Plants are known to produce a diverse group of natural metabolites with different biological activities.Centaurea ensiformis P.H.Davis,Origanum hypericifolium O.Schwartz&P.H.Davis,and Paeonia turcica Da... Background:Plants are known to produce a diverse group of natural metabolites with different biological activities.Centaurea ensiformis P.H.Davis,Origanum hypericifolium O.Schwartz&P.H.Davis,and Paeonia turcica Davis&Cullen are endemic plant species that grow on mountains in select regions in Türkiye and have been used in traditional Turkish medicine for various ailments.Methods:As first,we evaluated the larvicidal and antibiofilm activities of ethanol,ethyl acetate,acetone,and water extracts obtained from these plants.Antioxidant activities of the extracts were also investigated.Results:All tested extracts were effective at concentrations>25 ppm on Aedes aegypti larval mortality with the lethal concentration 50(LC_(50))values ranging between 32.82-48.35 ppm and LC90 between 46.26-63.2 ppm.O.hypericifolium was the most effective plant,ethanol extracts presented LC50 values of 32.82 ppm.Extracts demonstrated varying degrees of antibiofilm activity depending on the dose and bacterial species.Origanum hypericifolium extracts notably inhibited biofilms of Staphylococcus aureus(up to 98%inhibition),while P.turcica showed moderate efficacy against the same bacterial species.Pseudomonas aeruginosa biofilms displayed high resistance to all extracts.Conclusion:The results indicated that these endemic Turkish plants possess promising larvicidal and antibiofilm potential,particularly Origanum hypericifolium.Extracts analyzed by liquid chromatography-tandem mass spectrometry contained caffeic acid,myricetin,cinnamic acid,quercetin,gallic acid,epicatechin,and ascorbic acid.Further research should explore their potential applications in mosquito control and biofilm-related infections. 展开更多
关键词 ANTIOXIDANT ANTIBIOFILM LARVICIDAL Centaurea ensiformis Origanum hypericifolium paeonia turcica LC-MS/MS
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Genetic diversity and population genetic structure of Paeonia suffruticosa by chloroplast DNA simple sequence repeats(cpSSRs)
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作者 Qi Guo Xian Xue +5 位作者 Duoduo Wang Lixia Zhang Wei Liu Erqiang Wang Xiaoqiang Cui Xiaogai Hou 《Horticultural Plant Journal》 2025年第1期367-376,共10页
Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity le... Paeonia suffruticosa Andr.is an endemic shrub flower in China with 2n=10.This study used 228 cultivars from four populations,i.e.,Jiangnan,Japan,Northwest,and Zhongyuan,as materials to explore the genetic diversity levels among different populations of tree peony varieties.The results showed that 34 bands were amplified using five pairs of cp SSR primers,with an average of 6.8 bands per primer pair.The average number of different alleles(N_(a)),effective alleles(N_(e)),Shannon's information index(I),diversity(H),and polymorphic information content(PIC)were 3.600,2.053,0.708,0.433,and 0.388,respectively.The PIC value was between 0.250 and 0.500,indicating a moderate level of polymorphism for the five cp SSR primer pairs.The genetic diversity levels of peony cultivars varied among different populations,with the Northwest population showing relatively lower levels(I=0.590,H=0.289,and PIC=0.263).A total of 52 haplotypes were identified in the four examined populations,and the number of haplotypes per population ranged from 11 to 22.Forty-four private haplotypes were detected across populations,and the Northwest population exhibiting the highest count of private haplotypes with 17.The mean number of effective number of haplotypes(N_(eh)),haplotypic richness(R_(h)),and diversity(H)were 8.351,6.824,and 0.893,respectively.Analysis of molecular variance indicated that genetic variation within tree peony germplasm was greater than that between germplasm resources,and the main variation was found within individuals of peony germplasm.Cluster analysis,principal coordinate analysis,and genetic structure analysis classified tree peonies from different origins into two groups,indicating a certain degree of genetic differentiation among these four tree peony cultivation groups.This study provides a theoretical basis for the exploration,utilization,and conservation of peony germplasm resources,as well as for research on the breeding of excellent varieties. 展开更多
关键词 paeonia suffruticosa Chloroplast microsatellites(cp SSR) Genetic diversity Haplotypes Germplasm resources
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Protective mechanism of Paeoniae Radix Alba against chemical liver injury based on network pharmacology,molecular docking,and in vitro experiments
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作者 Shuangqiao Liu Xin Liu +7 位作者 Sijia Jiang Min Fu Jinxi Hu Jiaqi Liu Xiaoxu Fan Yingtong Feng Shujing Zhang Jingxia Wang 《Journal of Traditional Chinese Medical Sciences》 CAS 2024年第1期55-66,共12页
Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell e... Objective:To explore and validate the potential targets of Paeoniae Radix Alba(P.Radix,Bai Shao)in protecting against chemical liver injury through network pharmacology,molecular docking technology,and in vitro cell experiments.Methods:Network pharmacology was used to identify the common potential targets of P.Radix and chemical liver injury.Molecular docking was used to fit the components,which were subsequently verified in vitro.A cell model of hepatic fibrosis was established by activating hepatic stellate cell(HSC)-LX2 cells with 10 ng/mL transforming growth factor-β1.The cells were exposed to different concentrations of total glucosides of paeony(TGP),the active substance of P.Radix,and then evaluated using the cell counting kit-8 assay,enzyme-linked immunosorbent assay,and western blot.Results:Analysis through network pharmacology revealed 13 key compounds of P.Radix,and the potential targets for preventing chemical liver injury were IL-6,AKT serine/threonine kinase 1,jun protooncogene,heat shock protein 90 alpha family class A member 1(HSP90AA1),peroxisome proliferator activated receptor gamma(PPARG),PTGS2,and CASP3.Gene Ontology(GO)enrichment analysis indicated the involvement of response to drugs,membrane rafts,and peptide binding.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis revealed that the main pathways involved lipid and atherosclerosis and chemical carcinogenesis-receptor activation.Paeoniflorin and albiflorin exhibited strong affinity for HSP90AA1,PTGS2,PPARG,and CASP3.Different concentrations of TGP can inhibit the expression of COL-I,COL-III,IL-6,TNF-a,IL-1β,HSP-90a,and PTGS2 while increasing the expression of PPAR-γand CASP3 in activated HSC-LX2 cells.Conclusion:P.Radix primarily can regulate targets such as HSP90AA1,PTGS2,PPARG,CASP3.TGP,the main active compound of P.Radix,protects against chemical liver injury by reducing the inflammatory response,activating apoptotic proteins,and promoting the apoptosis of activated HSCs. 展开更多
关键词 paeoniae Radix Alba Total glycosides of paeony Chemical liver injury Liver fibrosis Network pharmacology Hepatic stellate cells
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凤丹(Paeonia ostii T.)自毒物质的检测及其作用机制 被引量:35
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作者 覃逸明 聂刘旺 +3 位作者 黄雨清 王千 刘欣 周科 《生态学报》 CAS CSCD 北大核心 2009年第3期1153-1161,共9页
药用牡丹的种植中存在明显的连作障碍现象,为探讨其机制,利用已萌发长根的凤丹(Paeonia ostii)种子对凤丹根际土壤和根的水浸提液进行了自毒化感检测;采用高效液相色谱法(HPLC)对种植4a的凤丹根际土壤及其根皮中的化感自毒物质进行了定... 药用牡丹的种植中存在明显的连作障碍现象,为探讨其机制,利用已萌发长根的凤丹(Paeonia ostii)种子对凤丹根际土壤和根的水浸提液进行了自毒化感检测;采用高效液相色谱法(HPLC)对种植4a的凤丹根际土壤及其根皮中的化感自毒物质进行了定性和定量分析;在此基础上,利用外源法研究了盆栽凤丹幼苗对5种检出物质(阿魏酸、肉桂酸、香草醛、香豆素和丹皮酚)及其混合物在3种不同浓度下对植株生长的影响和生理响应。结果表明:(1)连续种植4a的凤丹根际土壤和根浸提液对已萌发种子根的生长有显著的抑制作用(p<0.05),显示凤丹根分泌物具有自毒化感的潜势;(2)HPLC分析表明在凤丹根际土壤中存在5种以上的酚酸类物质(阿魏酸、肉桂酸、香草醛、香豆素和丹皮酚),其中阿魏酸、肉桂酸、香草醛、香豆素为前人已报道的化感物质;(3)在实验室条件下,所设浓度范围内,5种物质及其混合物对凤丹幼苗的高度、根长及地下和地上生物量均有明显影响,尤其对根长和地下生物量的影响最为明显(p<0.05),此结果与连作移栽幼苗时,根部首先发黑死亡的现象相一致;(4)凤丹幼苗的根系活力和叶绿素含量在各处理中表现出相似性,即低浓度时根系活力和叶绿素含量高,高浓度时根系活力和叶绿素含量低,低浓度的阿魏酸和香草醛对幼苗根系活力和叶绿素合成有促进作用。此结果显示自毒物质可能是通过影响根系酶活性和叶绿素合成来影响群体中其它个体的生长。本实验的结果显示栽培4a的凤丹土壤水提液具有抑制自身种子根系生长的活性,此提示栽培凤丹连作障碍可能主要与自身分泌到土壤中的酚酸类物质的自毒作用有关。 展开更多
关键词 凤丹(paeonia ostii T.Hong ET J.X.Zhang) 自毒作用 自毒物质 作用机制
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Paeonia suffruticosa Andrews的界定,兼论栽培牡丹的分类鉴定问题 被引量:18
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作者 洪德元 潘开玉 周志钦 《植物分类学报》 CSCD 北大核心 2004年第3期275-283,共9页
栽培牡丹一直称为PaeoniasuffruticosaAndrews。在把作丹皮用的栽培牡丹 (凤丹 )描述为新种P .ostii后 ,观赏的栽培牡丹仍然被统称为P .suffruticosa。但在P .suffruticosa复合体内 ,P .rockii,P .jis hanensis,P .qiui和P .suffruticos... 栽培牡丹一直称为PaeoniasuffruticosaAndrews。在把作丹皮用的栽培牡丹 (凤丹 )描述为新种P .ostii后 ,观赏的栽培牡丹仍然被统称为P .suffruticosa。但在P .suffruticosa复合体内 ,P .rockii,P .jis hanensis,P .qiui和P .suffruticosassp .yinpingmudan等野生类型相继被描述并得到承认。于是 ,有人提出了栽培牡丹多元发生的观点 ,同时也出现了P .suffruticosa“是牡丹组数种植物复合体”的说法。本文在详细论述P .suffruticosa复合体分类历史的基础上 ,根据我们自己对野生类型和栽培类型的考察研究 ,认为观赏栽培牡丹的形成是多元的 ,并且包含几个物种 ;P .suffruticosa有明确的概念 ,它是观赏栽培牡丹的组成部分 ,而不代表栽培牡丹的全部 ,更不是数种植物的复合体。我们认为应根据形态特征正确鉴定观赏栽培牡丹 ,不应把它们一概鉴定为P .suffruticosa。通过文献和标本引证 ,文中界定了P .suf fruticosa的范围。此外 ,把P .yinpingmudan (D .Y .Hong,K .Y .Pan&Z .W .Xie)B .A .Shen作为P .suffruticosassp.yinpingmudanD .Y .Hong,K .Y .Pan&Z .W .Xie的异名 ,并且认定P .yinpingmudan (D .Y .Hong ,K .Y .Pan&Z .W .Xie)B .A .Shenssp .henanensis(D .Y .Hong,K .Y .Pan&Z .W .Xie)B .A .Shen是不? 展开更多
关键词 栽培牡丹 paeonia suffruticosa Andrews 凤丹 牡丹 牡丹复合体 分类 鉴定
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Monoterpene Glycosides from the Roots of Paeonia lactiflora 被引量:7
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作者 Xiao Ling WANG Wei JIAO +2 位作者 Xun LIAO Shu Ling PENG Li Sheng DING 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第7期916-918,共3页
A new monoterpene glycoside, together with nine known ones, 3-O-methylpaeoniflorin, mudanpioside J, paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoyloxypaeoniflorin, oxybenzoylpaeoniflorin, albiflorin and la... A new monoterpene glycoside, together with nine known ones, 3-O-methylpaeoniflorin, mudanpioside J, paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoyloxypaeoniflorin, oxybenzoylpaeoniflorin, albiflorin and lactiflorin, was isolated from the roots of Paeonia lactiflora Pall.. The structure of the new compound was elucidated as galloylalbiflorin by the spectro- scopic evidence including ESI-MS, 1D- and 2D-NMR spectra. 展开更多
关键词 paeonia lactiflora paeoniaceae monoterpene glycoside galloylalbiflorin.
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Paeonivayin, A New Monoterpene Glycoside from Paeonia delavayi 被引量:7
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作者 Yun Bao MA Da Gang WU Ji Kai LIU (Department of Phytochemistry,Kunming Institute of Botany.The Chinese Academy of Sciences.Kunming 650204) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第9期771-774,共4页
A new monoterpene glycoside named paeonivayin with other seven known compounds were isolated from the roots of Paeonia delavayi Franch. and their structures were determined by means of spectroscopic studies.
关键词 paeonia delavayi Franch paeoniaceae monoterpene glycoside paeonivayin
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几种化感物质对凤丹(Paeonia ostii)生长的影响 被引量:2
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作者 覃逸明 黄雨清 +2 位作者 王千 刘欣 周科 《柳州师专学报》 2010年第4期120-125,129,共7页
通过盆栽实验研究了5种化感物质阿魏酸、肉桂酸、香草醛、香豆素和丹皮酚及其混合物在不同浓度下对凤丹生长的影响。结果表明:5种化感物质及其混合物对凤丹茎高和地下部生物量有明显影响,对茎径生长没有明显影响,高浓度的混合物和香豆... 通过盆栽实验研究了5种化感物质阿魏酸、肉桂酸、香草醛、香豆素和丹皮酚及其混合物在不同浓度下对凤丹生长的影响。结果表明:5种化感物质及其混合物对凤丹茎高和地下部生物量有明显影响,对茎径生长没有明显影响,高浓度的混合物和香豆素抑制效果最明显;低浓度的阿魏酸和香草醛对凤丹生长有促进作用,表现出"低促高抑"的现象。 展开更多
关键词 化感物质 替代品 凤丹(paeonia ostii T.Hong ET J.X.Zhang) 生长 化感作用
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A New 24, 30-Dinortriterpenoid from Paeonia delavayi 被引量:5
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作者 Shao Hua WU Xiao Dong LUO +2 位作者 Yun Bao MA Xiao Jiang HAO Da Gang WU 《Chinese Chemical Letters》 SCIE CAS CSCD 2001年第4期345-346,共2页
A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl... A new triterpenoid. 3 beta. 4 beta. 23-trihydroxy-24, 30-dinorolean-12. 20(29)-dien-28-oic acid. together with five known compounds. 2 alpha. 3 beta. 23-trihydroxy-12-oleanen-28-oic acid-beta -D-glucose glucopyranosyl ester. palbinone. 2-hydroxy-benzoic acid, vanillic acid. syringic acid. were isolated from the roots of Paeonia delavayi Franch. Their structures were characterized by spectral analysis. 展开更多
关键词 paeonia delavayi Franch. paeoniaceae TRITERPENOID 3 beta 4 beta 23-trihydroxy-24 30-dinorolean-12 20(29)-dien-28-oic acid
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A New Monoterpene Glycoside from Paeonia veitchii 被引量:2
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作者 Shao Hua WU Xiao Dong LUO +2 位作者 Yun Bao MA Xiao Jiang HAO Da Gang WU 《Chinese Chemical Letters》 SCIE CAS CSCD 2002年第5期430-431,共2页
A new monoterpene glycoside, acetoxypaeoniflorin, was isolated from the root cortex of Paeonia veitchii Lynch.. The structure was elucidated by spectral methods.
关键词 paeonia veitchii Lynch paeoniaceae monoterpene glycoside acetoxypaeoniflorin.
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Mechanisms involved in antineuralgic effects of Paeonia Lactiflora: prediction based on network pharmacology 被引量:9
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作者 Di Zhang Shengsuo Ma +5 位作者 Jianxin Sun Bing Yang Haoming Lin Meijing Xie Meina Huang Guoping Zhao 《TMR Clinical Research》 2019年第2期43-56,共14页
Objective: The analgesic effect of Paeonia Lactiflora has been widely accepted in traditional Chinese medicine. But little is known about the potential mechanism. This study aims to elucidate the effective components ... Objective: The analgesic effect of Paeonia Lactiflora has been widely accepted in traditional Chinese medicine. But little is known about the potential mechanism. This study aims to elucidate the effective components and analgesic mechanism based on network pharmacology. Methods: TCMSP was screened to collect the possible active ingredients and their CAS and SMILES was searched in Pubchem and further be used for reverse molecular docking in Swiss Target Prediction database to obtain potential targets. Pain-related molecules were obtained from GeenCards database, and the predicted targets of Paeonia Lactiflora for pain treatment were selected by Wayne diagram. For mechanism analysis, the protein-protein interactions were constructed by String, the GO analysis and KEGG analysis were conducted in DAVID. Results: Through GO analysis and KEGG analysis, we found that the pain related signaling pathways mainly involved in serotonergic synapse, calcium signaling pathway, inflammatory mediator TRP channels. Using network-based systems biology and molecular docking analyses, we predicted that 11 active ingredients in Paeonia Lactiflora has the analgesic effects with 97 potential targets. PRKCA, CASP3, ALOX15, SLC6A4, PRKCG, ALOX5, PRKCB, ALOX12, EGFR, ADRB2, RYR3, RYR1, NOS2, PTAFR, PRKCQ, and PRKCD were involved in the analgesic effects of Paeonia Lactiflora. Conclusion: Paeonia Lactiflora may alleviate pain through inflammatory mediator regulation of TRP channels, Ca2+ signaling pathway and 5-HT receptor. PRKCA, PRKCB, PRKCD,PRKCQ, and PRKCG may be new targets for pain treatment. 展开更多
关键词 paeonia lactiflora paeonia network pharmacology inflammatory mediator regulation of TRP channels
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Evaluation of Baishao(Radix Paeoniae Alba)and Chishao(Radix Paeoniae Rubra)from different origins based on characteristic spectra of amino acids 被引量:2
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作者 LI Shasha ZHANG Yiping +4 位作者 DENG Yuanhui WANG Qian XIE Zhiru Ibarra-Estrada Emmanuel XIAO Xue 《Digital Chinese Medicine》 2021年第2期120-129,共10页
Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of ... Objective To evaluate the difference between Baishao(Paeoniae Radix Alba,PRA)and Chishao(Paeoniae Radix Rubra,PRR)from different regions based on the characteristic spectra of amino acids(AAs).Methods Fingerprints of the 21 standard AAs were established using O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivation method.The AA components in PRA and PRR were characterized qualitatively and quantitatively,and different AAs were screened using pattern recognition technology.Results Twelve AAs were identified in both PRA and PRR.Meanwhile,aspartic acid,glutamic acid,arginine,alanine,and gamma-aminobutyric acid were screened as characteristic components,and their concentrations could effectively distinguish PRA from PRR.Conclusion The established characterization method,which is based on the characteristic spectra of AAs,is accurate,efficient,and sensitive and can effectively distinguish between PRA and PRR from different producing areas,thus providing a reference for the overall characterization and evaluation of Chinese medicinal materials. 展开更多
关键词 O-phthalaldehyde-9-fluorenylmethyl chloroformate(OPA-FMOC)pre-column derivatization method Amino acids characteristic spectra Baishao(paeoniae Radix Alba PRA) Chishao(paeoniae Radix Rubra PRR) Pattern recognition
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Analysis of the Volatile Components in Flowers of <i>Paeonia lactiflora</i>Pall. and <i>Paeonia lactiflora</i>Pall. var. <i>Trichocarpa</i> 被引量:1
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作者 Tonglin Wang Anqi Xie +4 位作者 Dongliang Zhang Zemiao Liu Xue Li Yang Li Xia Sun 《American Journal of Plant Sciences》 2021年第1期146-162,共17页
<i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. <... <i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:""><span style="font-family:Verdana;"> Pall. var. </span><i><span style="font-family:Verdana;">trichocarpa </span></i><span style="font-family:Verdana;">is a variety of </span><i><span style="font-family:Verdana;">Paeonia lactiflora</span></i><span style="font-family:Verdana;"> Pall., and is currently the peony herb</span></span><span style="font-family:Verdana;">’</span><span style="font-family:""><span style="font-family:Verdana;">s principal cultivar group. Here, we study the differences in aromatic components and flowers of different varieties between two groups of cultivars, providing a reference for applying natural fragrance substances of peonies, breeding fragrant flower types, and developing and </span><span style="font-family:Verdana;">using improved varieties. Headspace solid-phase microextraction (HS-SPME),</span> <span style="font-family:Verdana;">gas chromatography-mass spectrometry (GC-MS), peak area normalization for</span><span style="font-family:Verdana;"> each component relative to content, component library (NIST14/NIST14S) retrieval, and a literature review were used to analyze the volatile compounds in flowers of eight peony varieties, such as </span></span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Gaoganhong</span><span style="font-family:Verdana;">”</span><span style="font-family:Verdana;">, and ten comospore peony varieties, such as </span><span style="font-family:Verdana;">“</span><span style="font-family:Verdana;">Jinshanhong</span><span style="font-family:Verdana;">”</span><span style="font-family:""><span style="font-family:Verdana;">. Results showed that the main volatile compound constituents in flowers of the two groups were terpenes and alcohols. Additionally, the content of eucalyptol, caryophyllene, α-Pinene, citronellol, and 3-Hexen-1-ol, acetate, (Z) was high. Peony cultivars contained linalool,</span><span style="font-family:Verdana;"> (1R)-2,6,6-trimethylbicyclo[3.1.1]hept-2-ene, and 1,4-dimethoxybenzene,</span><span style="font-family:Verdana;"> while comospore peony varieties contained 1,3,6-octatriene, 3,7-dimethyl-, (Z)-, phenylethyl alcohol, and geraniol. In this study, the differences between the volatile components of flowers of different peony varieties were clarified, laying a foundation for further molecular biology research into the floral fragrance of peonies and the cultivation of new varieties of aromatic peonies. At the same time, it also provides a theoretical basis for the development and application of peony flower by-products. 展开更多
关键词 paeonia lactiflora Pall. paeonia lactiflora Pall. var. Trichocarpa GC-MS Volatile Components
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Paeonia anomala的核型研究(英文) 被引量:2
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作者 潘锦 张大明 +3 位作者 王超 桑涛 潘开玉 洪德元 《云南植物研究》 CSCD 北大核心 2006年第5期488-492,共5页
报道了Paeonia anomalaL.的核型,澄清了新疆阿尔泰地区分布的该物种的染色体数目。分布于该地区的Paeonia anomalaL.的核型组成:2n=2x=10=6m+2sm+2st。该类群核型与该属其它类群一致———2A型。在综合比较分析该属染色体参数以及核型... 报道了Paeonia anomalaL.的核型,澄清了新疆阿尔泰地区分布的该物种的染色体数目。分布于该地区的Paeonia anomalaL.的核型组成:2n=2x=10=6m+2sm+2st。该类群核型与该属其它类群一致———2A型。在综合比较分析该属染色体参数以及核型不均一性参数,包括最长/最短染色体比(L1/Ln)和染色体不对称系数(CKOA)的基础上,我们发现该属三个组在核型上没有明显分化,仅在木本类群(Sect.MoutanDC.)和草本类群(Sect.OnaepiaLindley和Sect.Paeonia)之间存在微小差异。此外,作为二倍体类群,新疆阿尔泰地区分布的Paeonia anomalaL.很可能是二倍体杂种,这将为研究被子植物的父母本同倍化杂交式物种起源提供一个很好的研究材料。 展开更多
关键词 paeonia paeonia ANOMALA 核型 染色体不对称系数
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白芍(Radix paeoniae alba)提取物对中华绒螯蟹(Eriocheir sinensis)造血组织发育及造血因子Astakine的影响 被引量:2
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作者 吕孙建 焦锦彪 +6 位作者 刘莉 袁雪梅 于喆 张海琪 杭小英 施伟达 吴颖蕾 《海洋与湖沼》 CAS CSCD 北大核心 2021年第3期754-761,共8页
血淋巴细胞在甲壳动物免疫反应中起着非常重要的作用,其主要由造血组织(Hematopoietic tissue,HPT)生成并随着开管式循环分布到身体各处。到目前为止,还没有相关研究表明甲壳动物造血组织是否受草本植物的影响。本研究以中华绒螯蟹(Erio... 血淋巴细胞在甲壳动物免疫反应中起着非常重要的作用,其主要由造血组织(Hematopoietic tissue,HPT)生成并随着开管式循环分布到身体各处。到目前为止,还没有相关研究表明甲壳动物造血组织是否受草本植物的影响。本研究以中华绒螯蟹(Eriocheir sinensis)为对象,利用荧光定量PCR、免疫荧光和组织化学等技术,初步探索了白芍(Radix paeoniae alba)提取物对中华绒螯蟹造血组织和造血因子Astakine的影响。结果显示,在原代培养的造血组织细胞中添加终浓度为0.85 mg/mL的白芍提取物后,造血因子Astakine mRNA的表达量显著上调(P<0.05)。进一步制备含1%白芍提取物的中华绒螯蟹饲料并进行饲喂实验,结果显示饲喂含白芍提取物饲料的中华绒螯蟹造血组织的Astakine mRNA的表达量较饲喂前上调了10.5倍,要高于对照组。免疫荧光结果同样表明,饲喂含白芍提取物饲料后中华绒螯蟹造血组织的Astakine蛋白荧光信号也要强于对照组。通过造血组织的形态学观察和造血组织体重百分比的检测,我们发现白芍组中华绒螯蟹造血组织含有较多卵形小叶,且造血组织体重百分比较饲喂前增加了6.64%,而对照组仅增加1.6%。免疫保护率实验显示,在脂多糖急性刺激下,白芍添加组的中华绒螯蟹的存活率(77.5%)要高于对照组(57.5%)。综上所述,白芍提取物可以促进中华绒螯蟹造血因子的表达和造血组织的发育,从而提高中华绒螯蟹的免疫调节功能。 展开更多
关键词 白芍(Radix paeoniae alba)提取物 中华绒螯蟹(Eriocheir sinensis) 造血组织 造血因子
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芍药属牡丹组(Paeonia sect.Moutan)种间关系的分子证据:GPAT基因的PCR-RFLP和序列分析 被引量:34
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作者 赵宣 周志钦 +2 位作者 林启冰 潘开玉 洪德元 《植物分类学报》 CSCD 北大核心 2004年第3期236-244,共9页
为了探讨芍药属牡丹组Paeoniasect.Moutan的种间关系 ,对采自 1 5个野生居群 ,代表牡丹组全部 8个野生种的 1 5份材料的GPAT基因片段 (外显子 5和 6之间 2kb的内含子 )进行了PCR_RFLP分析 ,并对代表牡丹组全部 8个野生种的 9份材料进行... 为了探讨芍药属牡丹组Paeoniasect.Moutan的种间关系 ,对采自 1 5个野生居群 ,代表牡丹组全部 8个野生种的 1 5份材料的GPAT基因片段 (外显子 5和 6之间 2kb的内含子 )进行了PCR_RFLP分析 ,并对代表牡丹组全部 8个野生种的 9份材料进行了测序。根据 1 2个限制性内切酶的PCR_RFLP数据 ,使用Network 3 .0计算机程序的RM (reduced_median)法建立了牡丹组种间亲缘关系网络树。同时根据 8个种 9份材料的GPAT基因片段序列 ,利用PAUP 4.0计算机程序建立了牡丹组GPAT基因的最大简约(MP)树和邻接 (NJ)树。结果获得了具有很高自展值支持、分辨良好的牡丹组种间关系 (GPAT基因 )树。最重要的是 ,该基因树所显示的牡丹组种间关系与根据形态学证据提出的牡丹组的种间关系基本吻合 ,并得到其他研究证据的支持。根据这一结果 ,对牡丹组的种间关系进行了详细的讨论。 展开更多
关键词 芍药属 牡丹组 野生居群 种间关系 GPAT基因 PCR-RFLP 序列分析
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濒危植物大花黄牡丹(Paeonia ludlowii)种群数量动态 被引量:54
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作者 杨小林 王秋菊 +1 位作者 兰小中 李春燕 《生态学报》 CAS CSCD 北大核心 2007年第3期1242-1247,共6页
运用种群静态生命表、存活曲线、生殖力表和Leslie矩阵模型,研究了西藏特有的濒危植物大花黄牡丹种群数量动态过程。静态生命表和种群存活曲线反映出大花黄牡丹在树龄10a之前和20~25a之间分别经历了强烈的环境筛和竞争自疏,20a左右为其... 运用种群静态生命表、存活曲线、生殖力表和Leslie矩阵模型,研究了西藏特有的濒危植物大花黄牡丹种群数量动态过程。静态生命表和种群存活曲线反映出大花黄牡丹在树龄10a之前和20~25a之间分别经历了强烈的环境筛和竞争自疏,20a左右为其生理寿命,35a左右为极限寿命;大花黄牡丹种群的净增长率、内禀增长率和周限增长率较低,表现为衰退型种群;Leslie矩阵模型的模拟结果表明,在20a内种群幼苗数量和总数量呈现出下降趋势,下降了大约30%;大花黄牡丹种群存活表现为Deevey-Ⅰ型,早期个体死亡极高,幼龄苗木严重不足;现行种群数量主要是靠自身的萌蘖繁殖来维持。导致大花黄牡丹濒危的可能原因是大花黄牡丹的生物学特性以及人为干扰。 展开更多
关键词 濒危植物 大花黄牡丹 种群动态 Leslie模型
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药用植物凤丹(Paeonia suffruticosa)根际土壤细菌群落16S rRNA基因的ARDRA分析 被引量:12
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作者 张永敢 赵娟 +3 位作者 张玉洁 吴婷 吴孝兵 郑艳 《生态学报》 CAS CSCD 北大核心 2016年第17期5564-5574,共11页
凤丹(Paeonia suffruticosa Andr.)为芍药科多年生植物,是一种重要的传统中药资源。在其生长发育的周期中与土壤微生物尤其是根际土壤微生物有密切的关系。通过构建16S rRNA基因克隆文库及文库的限制性片段长度多态性分析(ARDRA),对中... 凤丹(Paeonia suffruticosa Andr.)为芍药科多年生植物,是一种重要的传统中药资源。在其生长发育的周期中与土壤微生物尤其是根际土壤微生物有密切的关系。通过构建16S rRNA基因克隆文库及文库的限制性片段长度多态性分析(ARDRA),对中国药用植物凤丹5大主要分布区域的根际土壤细菌群落多样性进行了研究。采用限制性内切酶HinfI和Csp6I对克隆文库中随机挑选的1000个白色克隆子进行了酶切分型,根据酶切图谱的不同,将其分为324个OTUs,并对38个优势OTUs进行了测序和系统发育分析。16S rRNA基因序列分析结果表明,凤丹根际土壤细菌种群主要包括:变形菌门(包括alpha、beta、gamma、detla亚门)、酸杆菌门、放线菌门、拟杆菌门及厚壁菌门等11类细菌,此外还包含了3个未归类的细菌。变形菌门和酸杆菌门为文库中的主要菌群,分别占克隆总数的47.34%和14.36%,其中Pseudomonas sp.、Burkholderia sp.和Arthrobacter sp.为优势菌属。研究结果表明,我国药用植物凤丹5大主要分布区域的根际土壤细菌种群不仅具有丰富的多样性,还存在丰富的潜在新菌种。 展开更多
关键词 凤丹 根际土壤 细菌 多样性 ARDRA
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Development Mode and Countermeasures of Paeonia suffruticosa Andr Industrialization 被引量:2
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作者 白晔 齐天真 《Agricultural Science & Technology》 CAS 2016年第9期2212-2215,共4页
The research introduced Paeonia suffruticosa industry from market demand, value and adaptability and its characteristics, providing references for Paeonia suffruticosa industrialization from product aspect. Finally, i... The research introduced Paeonia suffruticosa industry from market demand, value and adaptability and its characteristics, providing references for Paeonia suffruticosa industrialization from product aspect. Finally, its development models were proposed, as well as countermeasures, providing suggestions and countermeasures for Paeonia suffruticosa industry. 展开更多
关键词 paeonia suffruticosa Andr Industrial mode Countermeasures and suggestions
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芍药(PaeoniaL.)生物学特性研究进展 被引量:17
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作者 王莹 胡宝忠 《东北农业大学学报》 CAS CSCD 2004年第6期759-763,共5页
综述了近年来国内外芍药的研究进展概况,系统地介绍了芍药的起源、分布与分类,栽培与繁殖,组织培养,生殖生物学等方面的研究现状及发展方向。
关键词 芍药 起源 分类 栽培 组织培养 生殖生物学
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