Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the se...Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the severity of the disease,but its role in AD development remains elusive.Using single-cell RNA sequencing,we uncovered that keratinocytes activate a distinct immune response characterized by induction of Il24 when exposed to methicillin-resistant S.aureus(MRSA).Further experiments using animal models showed that the administration of recombinant IL-24 protein worsened AD-like pathology.Genetic ablation of Il24 or the receptor Il20rb in keratinocytes alleviated allergic inflammation and atopic march.Mechanistically,IL-24 acted through its heterodimeric receptors on keratinocytes and augmented the production of IL-33,which in turn aggravated type 2 immunity and AD-like skin conditions.Overall,these findings establish IL-24 as a critical factor for onset and progression of AD and a compelling therapeutic target.展开更多
目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2...目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。展开更多
Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agent...Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agents.Approximately 119 compounds from 90 plants were recognized as potent anti-bacterials[2].Dalbergia odorifera,a traditional Chinese plant,has demonstrated anti-tumor,anti-microbial,anti-inflammatory,and cardiovascular protective effects[3].Limited studies have explored D.odorifera flavonoids'inhibitory activity against MRSA.Transcriptomics,a high-throughput method,aided in comprehending plant antibacterial therapy by generating data for gene expression,target identification,and pathway analysis[4].Consequently,our study aimed to assess D.odorifera's anti-MRSA effects and reveal its material foundation and antibacterial mechanism by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS),and transcriptomic analysis,in vitro,and in-silico studies.展开更多
MRSA早在本世纪60年代便成为医院的致病原,继而在全世界范围内成为医院感染的一个重要因素。在1992年的European Prevalence of infection in Intensive Care(EPIC)研究中(1000例病人。17个国家)表明医院感染的金葡菌中有2/3为MRSA株。...MRSA早在本世纪60年代便成为医院的致病原,继而在全世界范围内成为医院感染的一个重要因素。在1992年的European Prevalence of infection in Intensive Care(EPIC)研究中(1000例病人。17个国家)表明医院感染的金葡菌中有2/3为MRSA株。国内对MRSA感染已有报导。MRSA不但对甲氧西林耐药.展开更多
基金supported by the National Key R&D Program of China(2023YFC2306300)the National Natural Science Foundation of China(Grant Nos.32225019,92357304,and 32394003)supported by the Center for Life Sciences,the Institute for Immunology,and School of Basic Medical Sciences at Tsinghua University.
文摘Atopic dermatitis(AD)is a prevalent inflammatory skin disorder in which patients experience recurrent eczematous lesions and intense itching.The colonization of Staphylococcus aureus(S.aureus)is correlated with the severity of the disease,but its role in AD development remains elusive.Using single-cell RNA sequencing,we uncovered that keratinocytes activate a distinct immune response characterized by induction of Il24 when exposed to methicillin-resistant S.aureus(MRSA).Further experiments using animal models showed that the administration of recombinant IL-24 protein worsened AD-like pathology.Genetic ablation of Il24 or the receptor Il20rb in keratinocytes alleviated allergic inflammation and atopic march.Mechanistically,IL-24 acted through its heterodimeric receptors on keratinocytes and augmented the production of IL-33,which in turn aggravated type 2 immunity and AD-like skin conditions.Overall,these findings establish IL-24 as a critical factor for onset and progression of AD and a compelling therapeutic target.
文摘目的:通过Meta分析研究骨髓炎患者中耐甲氧西林金黄色葡萄球菌(MRSA)的感染率。方法:检索PubMed、Web of Science、中国知网、维普、万方和中国生物医学文献数据库(CBM)关于骨髓炎患者MRSA感染率的研究,检索时限为建库至2023年8月。由2位研究人员按照纳入与排除标准对所获文献独立进行筛选、提取,采用纽卡斯尔-渥太华量表评分(NOS)进行文献质量评价。以感染率为结局指标合并数据,采用R(4.3.1)进行Meta分析,并根据骨髓炎类型、患者年龄、研究地区进行亚组分析。结果:共纳入26篇文献,共包含20860名骨髓炎患者。Meta分析结果显示,骨髓炎患者MRSA感染率为0.240(95%CI:0.183~0.297)。亚组分析结果显示,椎体骨髓炎(VO)患者、18~60岁、北美地区骨髓炎患者的MRSA感染率更高。结论:骨髓炎患者中MRSA的感染率较高,需采取有效的预防和控制措施减少MRSA的传播。
基金the National Natural Science Foundation of China(Grant No.:81973191)Shanghai Agriculture Applied Technology Development Program,China(Program No.:T20200104)the National Key Research and Development Program of China(Program No.:2018YFE0192600)for providing financial support for the complete undertaking.
文摘Methicillin-resistant Staphylococcus aureus(MRSA)is an important pathogen casting dire shadow over global human wellbeing[1].Rising antibiotic resistance in MRSA led to research into plant-derived anti-microbial agents.Approximately 119 compounds from 90 plants were recognized as potent anti-bacterials[2].Dalbergia odorifera,a traditional Chinese plant,has demonstrated anti-tumor,anti-microbial,anti-inflammatory,and cardiovascular protective effects[3].Limited studies have explored D.odorifera flavonoids'inhibitory activity against MRSA.Transcriptomics,a high-throughput method,aided in comprehending plant antibacterial therapy by generating data for gene expression,target identification,and pathway analysis[4].Consequently,our study aimed to assess D.odorifera's anti-MRSA effects and reveal its material foundation and antibacterial mechanism by ultra-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry(UPLC-Q-TOF-MS),and transcriptomic analysis,in vitro,and in-silico studies.
文摘MRSA早在本世纪60年代便成为医院的致病原,继而在全世界范围内成为医院感染的一个重要因素。在1992年的European Prevalence of infection in Intensive Care(EPIC)研究中(1000例病人。17个国家)表明医院感染的金葡菌中有2/3为MRSA株。国内对MRSA感染已有报导。MRSA不但对甲氧西林耐药.
