A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-wa...A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method;the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.展开更多
Firstly, the water-quality characteristics of intestinal lavage wastewater and enzymolysis wastewater from the typical heparin sodium pro- ductive process were analyzed, and then the segregation treatment was applied ...Firstly, the water-quality characteristics of intestinal lavage wastewater and enzymolysis wastewater from the typical heparin sodium pro- ductive process were analyzed, and then the segregation treatment was applied in the treatment of enteric coating and heparin sodium manufacturing wastewater. Finally, the treatment of the two kinds of wastewater by different methods were discussed. The results showed that, COD of enzymolysis wastewater treated by centrifugation-coagulation-Fenton reagent oxidation-adsorption process was lower than 100 mg/L, while intestinal lavage wastewater treated by coagulation-ASBR-SBR process could meet the first standard of Comprehensive Discharge Standard of Sewage （GB8978-1996） after one month of continuous operation.展开更多
目的探讨低分子肝素钠联合大量饮水对羊水过少孕妇宫腔环境和母婴结局的影响。方法采用随机数表法将上蔡县人民医院2022年1月至2024年10月收治的90例羊水过少孕妇随机分为研究组和对照组,各45例,研究组给予低分子肝素钠联合大量饮水治疗...目的探讨低分子肝素钠联合大量饮水对羊水过少孕妇宫腔环境和母婴结局的影响。方法采用随机数表法将上蔡县人民医院2022年1月至2024年10月收治的90例羊水过少孕妇随机分为研究组和对照组,各45例,研究组给予低分子肝素钠联合大量饮水治疗,对照组给予低分子肝素钠治疗。比较两组干预前后炎症因子水平、凝血功能指标、宫腔环境和围产期相关指标的差异,并在孕妇分娩后统计不良母婴结局的发生情况。结果干预后两组肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、超敏C反应蛋白(hs-CRP)、纤维蛋白原(FIB)、胎儿脉搏灌注指数(PI)、胎儿脐动脉血流收缩期最大血流速率/舒张末期血流速率(S/D)均较干预前降低,凝血酶原时间(PT)、凝血酶时间(TT)、羊水指数(AFI)较干预前升高,差异有统计学意义(P<0.05);两组TNF-α、IL-8、hs-CRP、PT、TT、FIB比较,差异无统计学意义(P>0.05);研究组PI、S/D低于对照组,AFI高于对照组,差异有统计学意义(P<0.05)。两组分娩方式占比比较,差异无统计学意义(P>0.05),研究组破膜时间长于对照组,研究组5 min Apgar评分为8~10分占比高于对照组,差异有统计学意义(P<0.05);研究组总不良母婴结局发生率为4.44%(2/45),对照组总不良母婴结局发生率为6.67%(3/45),两组比较,差异无统计学意义(P>0.05)。结论低分子肝素联合大量饮水能够显著改善羊水过少孕妇宫腔环境,降低孕妇体内炎症因子水平和胎膜早破风险,减少不良母婴结局的发生,且对孕妇的凝血功能产生的负面影响较小。展开更多
文摘A method to extract crude heparin sodium from pig intestinal mucosa by dialysis and spray drying was established. The pig intestinal mucosa was treated in the following steps: enzymolysis, resin exchange adsorption-washing, elution, pressure filtration, dialysis, spray drying. Activity of the product was measured using a heparin anti-IIa factor assay kit. The yield of crude heparin obtained by this method was 2.79% higher than that of oven drying method;the production of 1 kg crude heparin sodium saved 43.4 pigs small intestine. The activity was 98.48 ± 2.49 IU/mg (n = 5), 15.18 IU/mg higher than that obtained by oven drying method. The product is pale white powder, attractive color and easy to dissolve.
基金Supported by the Project of Practical Innovation Training Program of Undergraduates in Jiangsu Province in 2012(232)
文摘Firstly, the water-quality characteristics of intestinal lavage wastewater and enzymolysis wastewater from the typical heparin sodium pro- ductive process were analyzed, and then the segregation treatment was applied in the treatment of enteric coating and heparin sodium manufacturing wastewater. Finally, the treatment of the two kinds of wastewater by different methods were discussed. The results showed that, COD of enzymolysis wastewater treated by centrifugation-coagulation-Fenton reagent oxidation-adsorption process was lower than 100 mg/L, while intestinal lavage wastewater treated by coagulation-ASBR-SBR process could meet the first standard of Comprehensive Discharge Standard of Sewage （GB8978-1996） after one month of continuous operation.
文摘目的探讨低分子肝素钠联合大量饮水对羊水过少孕妇宫腔环境和母婴结局的影响。方法采用随机数表法将上蔡县人民医院2022年1月至2024年10月收治的90例羊水过少孕妇随机分为研究组和对照组,各45例,研究组给予低分子肝素钠联合大量饮水治疗,对照组给予低分子肝素钠治疗。比较两组干预前后炎症因子水平、凝血功能指标、宫腔环境和围产期相关指标的差异,并在孕妇分娩后统计不良母婴结局的发生情况。结果干预后两组肿瘤坏死因子-α(TNF-α)、白细胞介素-8(IL-8)、超敏C反应蛋白(hs-CRP)、纤维蛋白原(FIB)、胎儿脉搏灌注指数(PI)、胎儿脐动脉血流收缩期最大血流速率/舒张末期血流速率(S/D)均较干预前降低,凝血酶原时间(PT)、凝血酶时间(TT)、羊水指数(AFI)较干预前升高,差异有统计学意义(P<0.05);两组TNF-α、IL-8、hs-CRP、PT、TT、FIB比较,差异无统计学意义(P>0.05);研究组PI、S/D低于对照组,AFI高于对照组,差异有统计学意义(P<0.05)。两组分娩方式占比比较,差异无统计学意义(P>0.05),研究组破膜时间长于对照组,研究组5 min Apgar评分为8~10分占比高于对照组,差异有统计学意义(P<0.05);研究组总不良母婴结局发生率为4.44%(2/45),对照组总不良母婴结局发生率为6.67%(3/45),两组比较,差异无统计学意义(P>0.05)。结论低分子肝素联合大量饮水能够显著改善羊水过少孕妇宫腔环境,降低孕妇体内炎症因子水平和胎膜早破风险,减少不良母婴结局的发生,且对孕妇的凝血功能产生的负面影响较小。
