Add the masking agent and biscyclohexanoneoxalyldihydraone into the diluted clarificd liquid of the landfill leachate which was treatmented by digestion and centrifugal filtration to complexate all heavy metal ion in ...Add the masking agent and biscyclohexanoneoxalyldihydraone into the diluted clarificd liquid of the landfill leachate which was treatmented by digestion and centrifugal filtration to complexate all heavy metal ion in the trcatmented liquid, and extracted using CHCl3 many time, then it were demasking and decomposing respectively, and adjusted different pH and formed Me^n＋-PAN coordination compound when these metal ion reaction with PAN. The Fluoresence quenching of Rh6G （λex/λem=543mn/558nm） when the metal ion coordination compound was add into the Rh6G solution step by step, the quenching intensity was directly proportional to the concentration of the metal ion in the certain range. So a new method of fast and simple for determination of trace metal ion in landfill lcachate was established to determine metal ion in sample of different landfill leachate in Three Gorge Water Reservoir, and comparison the classic assay method with satisfactory results.展开更多
The fluorescence quenching of inclusion complex of neutral red （NR） and hydroxypropyl-β-cyclodextrin （HP-β-CD） carried by chlorobenzene was investigated. The fluorescence intensity of NR increased due to the for...The fluorescence quenching of inclusion complex of neutral red （NR） and hydroxypropyl-β-cyclodextrin （HP-β-CD） carried by chlorobenzene was investigated. The fluorescence intensity of NR increased due to the formed inclusion complex of HP-β-CD and NR. But the fluorescence intensity of NR-HP-β-CD diminished when chlorobenzene was added, and there was a linear relationship between the fluorescence quenching value of the system （△IF = IF, NR-HP-β-CD - IF, CB-NB-NR-HP-β-CD） and the concentration of chlorobenzene. Based on this, a novel fluorescence quenching method for the determination of chlorobenzene with NR as a fluorescence probe has been developed. Under the optimal conditions, the linear range of calibration curve for the determination of chlorobenzene was 5.0 × 10^-8 - 8.0 × 10^-6 mol/L and the detection limit was 1.0 × 10^-8 mol/L. It has been applied to determination ofchlorobenzene in synthetic waste water samples with satisfactory results.展开更多
The state of Tb3+ is investigated in liposome. When the concentration of PC is below CMC (critical micell concentration), most of Tb3+ is associated with PC, the binding constant is about 3.35×103 L/mol. When the...The state of Tb3+ is investigated in liposome. When the concentration of PC is below CMC (critical micell concentration), most of Tb3+ is associated with PC, the binding constant is about 3.35×103 L/mol. When the concentration of PC is beyond CMC, most of Tb3+ is dimerized, the dimerization constant is about 3.92×104L/mol. In PC?CH?H2O system, the binding constant of Tb3+?CH complex 2.93×104L/mol is obtained.展开更多
A fluoroimmunoassay method using unlabeled europium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simple because labeling process...A fluoroimmunoassay method using unlabeled europium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simple because labeling process is omitted.The detection limit is about 10^(10) mol/L antigen.The relative standard deviation of immunoassay is less than 10%.The recoveries of human serum albumin and estradiol protein conjugate are 96-105% and 111% respectively.展开更多
A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process ...A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process is unnecessary.The recovery of HSA and albumin in urine is 107% and 95% respectively.The standard deviation is tess than 10%.展开更多
Heteroatom doping is an efficient approach to regulate the fluorescence properties of carbon dots.Using aminophenylboronic acid as the raw material,a combustion method was developed for the synthesis of boron,nitrogen...Heteroatom doping is an efficient approach to regulate the fluorescence properties of carbon dots.Using aminophenylboronic acid as the raw material,a combustion method was developed for the synthesis of boron,nitrogen-doped carbon dots（B,N-carbon dots）.The B,N-carbon dots emitted green fluorescence and displayed high resistance to both photo bleaching and ionic strength.A facile fluorescence sensing approach for Cu^2＋ was fabricated via static fluorescence quenching.Under optimal conditions,a rapid detection of Cu^2＋ could be completed in 2 min with a linearity ranging from 1 μmol/L to 25 μmol/L and a detection limit of 0.3 μmol/L Furthermore,the proposed method showed potential applications for the detection of Cu^2＋ in natural water samples.展开更多
文摘Add the masking agent and biscyclohexanoneoxalyldihydraone into the diluted clarificd liquid of the landfill leachate which was treatmented by digestion and centrifugal filtration to complexate all heavy metal ion in the trcatmented liquid, and extracted using CHCl3 many time, then it were demasking and decomposing respectively, and adjusted different pH and formed Me^n＋-PAN coordination compound when these metal ion reaction with PAN. The Fluoresence quenching of Rh6G （λex/λem=543mn/558nm） when the metal ion coordination compound was add into the Rh6G solution step by step, the quenching intensity was directly proportional to the concentration of the metal ion in the certain range. So a new method of fast and simple for determination of trace metal ion in landfill lcachate was established to determine metal ion in sample of different landfill leachate in Three Gorge Water Reservoir, and comparison the classic assay method with satisfactory results.
基金This project was supported by the National Basic Research Program of China （No. 2007CB936602） and the Natural Science Foundation of Shandong Province in China （No. Y2008B20）.
文摘The fluorescence quenching of inclusion complex of neutral red （NR） and hydroxypropyl-β-cyclodextrin （HP-β-CD） carried by chlorobenzene was investigated. The fluorescence intensity of NR increased due to the formed inclusion complex of HP-β-CD and NR. But the fluorescence intensity of NR-HP-β-CD diminished when chlorobenzene was added, and there was a linear relationship between the fluorescence quenching value of the system （△IF = IF, NR-HP-β-CD - IF, CB-NB-NR-HP-β-CD） and the concentration of chlorobenzene. Based on this, a novel fluorescence quenching method for the determination of chlorobenzene with NR as a fluorescence probe has been developed. Under the optimal conditions, the linear range of calibration curve for the determination of chlorobenzene was 5.0 × 10^-8 - 8.0 × 10^-6 mol/L and the detection limit was 1.0 × 10^-8 mol/L. It has been applied to determination ofchlorobenzene in synthetic waste water samples with satisfactory results.
文摘The state of Tb3+ is investigated in liposome. When the concentration of PC is below CMC (critical micell concentration), most of Tb3+ is associated with PC, the binding constant is about 3.35×103 L/mol. When the concentration of PC is beyond CMC, most of Tb3+ is dimerized, the dimerization constant is about 3.92×104L/mol. In PC?CH?H2O system, the binding constant of Tb3+?CH complex 2.93×104L/mol is obtained.
基金This work was supported by National Natural Science Foundation of China.
文摘A fluoroimmunoassay method using unlabeled europium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simple because labeling process is omitted.The detection limit is about 10^(10) mol/L antigen.The relative standard deviation of immunoassay is less than 10%.The recoveries of human serum albumin and estradiol protein conjugate are 96-105% and 111% respectively.
基金supported by National Commission of Natural Science Foundation of China.
文摘A fluoroimmunoassay method using unlabeled Terbium chelate is described.The principle is similar to that of fluoroimmunoassay method using lanthanide chelate as labels.The procedure is simpte because labeling process is unnecessary.The recovery of HSA and albumin in urine is 107% and 95% respectively.The standard deviation is tess than 10%.
基金financially supported by the National Natural Science Foundation of China(No.21375112)the Marine hightech industry development projects of Fujian Province(No.2015-19)
文摘Heteroatom doping is an efficient approach to regulate the fluorescence properties of carbon dots.Using aminophenylboronic acid as the raw material,a combustion method was developed for the synthesis of boron,nitrogen-doped carbon dots（B,N-carbon dots）.The B,N-carbon dots emitted green fluorescence and displayed high resistance to both photo bleaching and ionic strength.A facile fluorescence sensing approach for Cu^2＋ was fabricated via static fluorescence quenching.Under optimal conditions,a rapid detection of Cu^2＋ could be completed in 2 min with a linearity ranging from 1 μmol/L to 25 μmol/L and a detection limit of 0.3 μmol/L Furthermore,the proposed method showed potential applications for the detection of Cu^2＋ in natural water samples.
