Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal pla...Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal plants namely,Achyranthes aspera(A.aspera) L.(Family Asparagaceae),Ficus caria(F.caria)(Family Moraceae).Malvi parviflora(M.parviflora)(Family Malvaceae),Vernonia species(V.species) docal name Alakit.Family Asteraceae),Solatium hastifolium(S.hastifolium)(Family Solanaceae),Calpurinia aurea(C.aurea)(Ait) Benth(Family Fabaceae),Nicotiana tabacum(N.tabacum) L.(Family Solanaceae),Ziziphus spina-christi(Z.spina-christi)(Family Rhamnaceae).Croton macroslachys(C.macrostachys)(Family Fuphorhiaceae).were screened against clinical bacterial isolates of veterinary importance from October 2007 to April2009.The antibacterial activity was tested using disc diffusion at two concentrations(200 mg/mL and 100 mg/mL) and broth dilution methods using 70%methanol macerated leal extracts.Results:With the exception ol S.hastifolium all plant extracts exhibited antibacterial activity.Among the medicinal plants tested C.aurea.C.macrostachyus,A.aspera,N.tabacum and vcrnonia species(Alakit) showed the most promising antimicrobial properties.Conclusions:It can be concluded that many of the tested plants have antibacterial activity and supports the traditional usage of the plants for mastitis,wound and gastrointestinal complications treatment.Further studies into their toxicity and phytochemistry is advocated.展开更多
Background: Detection of Methicillin Resistant Staphylococcus aureus may vary in phenotypic and genotypic methods. Objective: The aim of this present study was to validate the oxacillin screen agar test for the detect...Background: Detection of Methicillin Resistant Staphylococcus aureus may vary in phenotypic and genotypic methods. Objective: The aim of this present study was to validate the oxacillin screen agar test for the detection of methicillin-resistant Staphylococcus aureus isolated from clinical specimens. Methodology: This cross-sectional study was planned to carry out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University, Dhaka from January, 2010 to December, 2010 for a period of one (01) year. Different clinical specimens were collected from the patients presented with infections at anybody sites. Isolation and identification of Staphylococcus aureus (S. aureus) were performed by staining, culture and biochemical tests. Oxacillin screen agar test was performed to detect the methicillin-resistant Staphylococcus aureus. PCR was performed for detection of the mecA gene for MRSA. Result: Out of the 22 suspected MRSA isolates 19 were mecA positive by PCR. The sensitivity and specificity of oxacillin screen agar were 100.0% (95% CI 82.3 to 100.0%) and 33.3% (95% CI 24.0 to 90.6%) respectively. The positive predictive value and negative predictive value of oxacillin screen agar test were 90.5% (95% CI 81.0 to 95.5%) and 100.0% (95% CI 20.6 to 100.0%) respectively. The accuracy of the oxacillin screen agar test was 90.9% (95% CI 71.7 to 96.3%). Conclusion: In conclusion oxacillin screen agar test has high sensitivity with low specificity for the detection of MRSA.展开更多
<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study w...<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span style="font-family:Verdana;">Trichophyton mentagrophyte</span></i><span style="font-family:Verdana;"> 8 (25%), </span><i><span style="font-family:Verdana;">Trichophyton tonsurans</span></i><span style="font-family:Verdana;"> 5 (16%), Microsporum gypseum 3</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">(10%), </span><i><span style="font-family:Verdana;">Trichophyton verrucosum</span></i><span style="font-family:Verdana;"> 2 (6%), </span><i><span style="font-family:Verdana;">Trichophyton violaceum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">(3%) and </span><i><span style="font-family:Verdana;">Microsporum audouinii</span></i><span style="font-family:Verdana;"> 1 (3%). Out of 31 dermatophytes 17 were sensitive to all four antifungal agents within the range of FLC (2</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">6</span><span style="font-family:""> </span><span style="font-family:Verdana;">mcg/ml), ITR (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">2), TER (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">2), and GRI (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">2), 5 isolates were resistant in which 2 were resistant to FLC (64.256 mcg/ml), 2 isolates were resistant to TER 2 (32.38 mcg/ml), 1 isolate was resistant to both GRI and TER (16.32 mcg/ml) and 9 isolates were within the intermediate range. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">Every patient with a tinea infection should be properly studied for a mycological examination and should be treated accordingly. Dermatophyte test medium is more useful as an identification medium in the isolation of dermatophytes. The ABDD method appears to be a simple, cost-effective, and promising method for the evaluation of antifungal susceptibility of dermatophytes. E-test method is the most sensitive method due to the fact that quantitative MICs can be obtained directly from the E-strip. However, the E-test method is expensive and difficult in defining the precise borders of the inhibition zones in dermatophytes</span></span><span style="font-family:Verdana;">.展开更多
To performance susceptibility testing of antifungal agents Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance Methods We compared the results of s...To performance susceptibility testing of antifungal agents Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance Methods We compared the results of standard macrotube dilution reference method with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole Results Overall correlation between microdilution and macrodilution methods was 86% It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters Conclusion The disc diffusion test was evaluated as a low-cost, reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole展开更多
基金Ethiopian Ministry of Science and Technology research and development grant FVS/11/2008
文摘Objective:To screen the antibacterial activity of nine ethnoveterinary plants traditionally used for the treatment of mastitis,wound and gastrointestinal complications.Methods:Hydroalcoholic exctracts of medicinal plants namely,Achyranthes aspera(A.aspera) L.(Family Asparagaceae),Ficus caria(F.caria)(Family Moraceae).Malvi parviflora(M.parviflora)(Family Malvaceae),Vernonia species(V.species) docal name Alakit.Family Asteraceae),Solatium hastifolium(S.hastifolium)(Family Solanaceae),Calpurinia aurea(C.aurea)(Ait) Benth(Family Fabaceae),Nicotiana tabacum(N.tabacum) L.(Family Solanaceae),Ziziphus spina-christi(Z.spina-christi)(Family Rhamnaceae).Croton macroslachys(C.macrostachys)(Family Fuphorhiaceae).were screened against clinical bacterial isolates of veterinary importance from October 2007 to April2009.The antibacterial activity was tested using disc diffusion at two concentrations(200 mg/mL and 100 mg/mL) and broth dilution methods using 70%methanol macerated leal extracts.Results:With the exception ol S.hastifolium all plant extracts exhibited antibacterial activity.Among the medicinal plants tested C.aurea.C.macrostachyus,A.aspera,N.tabacum and vcrnonia species(Alakit) showed the most promising antimicrobial properties.Conclusions:It can be concluded that many of the tested plants have antibacterial activity and supports the traditional usage of the plants for mastitis,wound and gastrointestinal complications treatment.Further studies into their toxicity and phytochemistry is advocated.
文摘Background: Detection of Methicillin Resistant Staphylococcus aureus may vary in phenotypic and genotypic methods. Objective: The aim of this present study was to validate the oxacillin screen agar test for the detection of methicillin-resistant Staphylococcus aureus isolated from clinical specimens. Methodology: This cross-sectional study was planned to carry out in the Department of Microbiology and Immunology at Bangabandhu Sheikh Mujib Medical University, Dhaka from January, 2010 to December, 2010 for a period of one (01) year. Different clinical specimens were collected from the patients presented with infections at anybody sites. Isolation and identification of Staphylococcus aureus (S. aureus) were performed by staining, culture and biochemical tests. Oxacillin screen agar test was performed to detect the methicillin-resistant Staphylococcus aureus. PCR was performed for detection of the mecA gene for MRSA. Result: Out of the 22 suspected MRSA isolates 19 were mecA positive by PCR. The sensitivity and specificity of oxacillin screen agar were 100.0% (95% CI 82.3 to 100.0%) and 33.3% (95% CI 24.0 to 90.6%) respectively. The positive predictive value and negative predictive value of oxacillin screen agar test were 90.5% (95% CI 81.0 to 95.5%) and 100.0% (95% CI 20.6 to 100.0%) respectively. The accuracy of the oxacillin screen agar test was 90.9% (95% CI 71.7 to 96.3%). Conclusion: In conclusion oxacillin screen agar test has high sensitivity with low specificity for the detection of MRSA.
文摘<b><span style="font-family:Verdana;">Aim: </span></b><span style="font-family:""><span style="font-family:Verdana;">The objective of this study was to isolate, identify, and explore the </span><i><span style="font-family:Verdana;">in</span></i><span style="font-family:Verdana;">-</span><i><span style="font-family:Verdana;">vitro</span></i><span style="font-family:Verdana;"> antifungal susceptibility pattern of dermatophytes isolated from clinica</span><span style="font-family:Verdana;">lly suspected cases of dermatophytosis (tinea infections) attending th</span><span style="font-family:Verdana;">e Dermatology Department at J.S.S Hospital. </span><b><span style="font-family:Verdana;">Methods: </span></b><span style="font-family:Verdana;">This study was conducted at JSS Medical College and Hospital from December 2016 to December 2017. Clinical samples (e.g., skin scrapings and hair stumps) were collected under asep</span><span style="font-family:Verdana;">tic precautions. The identification of dermatophytes was perfor</span><span style="font-family:Verdana;">med</span><span style="font-family:Verdana;"> through microscopic examination using 10%, 20% & 40% potassium hydroxide (KOH) and culture on Sabouraud dextrose agar (SDA), SDAac, PDA and Dermatophyte test medium (DTM). All dermatophytes isolates were subjected to antifungal susceptibility testing using the agar-base</span><span style="font-family:Verdana;">d disk diffusion (ABDD) and E-test method against Terbinafine, Itraconazole, Fluconazole, and Griseofulvin. Data were analyzed by using Chi square test. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">A 100 samples were studied, 46% tinea corporis, 2%tinea cruris, 9% tinea pedis, 5% tin</span><span><span style="font-family:Verdana;">ea faciei. The dermatophytes isolated were </span><i><span style="font-family:Verdana;">Trichophyton rubrum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:Verdana;">1 (35%), </span><i><span style="font-family:Verdana;">Trichophyton mentagrophyte</span></i><span style="font-family:Verdana;"> 8 (25%), </span><i><span style="font-family:Verdana;">Trichophyton tonsurans</span></i><span style="font-family:Verdana;"> 5 (16%), Microsporum gypseum 3</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">(10%), </span><i><span style="font-family:Verdana;">Trichophyton verrucosum</span></i><span style="font-family:Verdana;"> 2 (6%), </span><i><span style="font-family:Verdana;">Trichophyton violaceum</span></i><span style="font-family:Verdana;"> 1</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">(3%) and </span><i><span style="font-family:Verdana;">Microsporum audouinii</span></i><span style="font-family:Verdana;"> 1 (3%). Out of 31 dermatophytes 17 were sensitive to all four antifungal agents within the range of FLC (2</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">6</span><span style="font-family:""> </span><span style="font-family:Verdana;">mcg/ml), ITR (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">2), TER (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:Verdana;">2), and GRI (0.125</span><span style="font-family:""> </span><span style="font-family:Verdana;">-</span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">2), 5 isolates were resistant in which 2 were resistant to FLC (64.256 mcg/ml), 2 isolates were resistant to TER 2 (32.38 mcg/ml), 1 isolate was resistant to both GRI and TER (16.32 mcg/ml) and 9 isolates were within the intermediate range. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">Every patient with a tinea infection should be properly studied for a mycological examination and should be treated accordingly. Dermatophyte test medium is more useful as an identification medium in the isolation of dermatophytes. The ABDD method appears to be a simple, cost-effective, and promising method for the evaluation of antifungal susceptibility of dermatophytes. E-test method is the most sensitive method due to the fact that quantitative MICs can be obtained directly from the E-strip. However, the E-test method is expensive and difficult in defining the precise borders of the inhibition zones in dermatophytes</span></span><span style="font-family:Verdana;">.
文摘To performance susceptibility testing of antifungal agents Due to the increasing number of resistant strains, susceptibility testing of antifungal agents is gaining importance Methods We compared the results of standard macrotube dilution reference method with two different microdilution methods, as well as the disc diffusion method in order to test the susceptibility of 150 Candida strains to fluconazole Results Overall correlation between microdilution and macrodilution methods was 86% It was 91% between the Minimal Inhibitory Concentrations obtained from macrodilution and disc diffusion zone diameters Conclusion The disc diffusion test was evaluated as a low-cost, reproducible, and efficient way of assessing the in vitro susceptibility of Candida strains to fluconazole
