Geological discontinuity(GD)plays a pivotal role in determining the catastrophic mechanical failure of jointed rock masses.Accurate and efficient acquisition of GD networks is essential for characterizing and understa...Geological discontinuity(GD)plays a pivotal role in determining the catastrophic mechanical failure of jointed rock masses.Accurate and efficient acquisition of GD networks is essential for characterizing and understanding the progressive damage mechanisms of slopes based on monitoring image data.Inspired by recent advances in computer vision,deep learning(DL)models have been widely utilized for image-based fracture identification.The multi-scale characteristics,image resolution and annotation quality of images will cause a scale-space effect(SSE)that makes features indistinguishable from noise,directly affecting the accuracy.However,this effect has not received adequate attention.Herein,we try to address this gap by collecting slope images at various proportional scales and constructing multi-scale datasets using image processing techniques.Next,we quantify the intensity of feature signals using metrics such as peak signal-to-noise ratio(PSNR)and structural similarity(SSIM).Combining these metrics with the scale-space theory,we investigate the influence of the SSE on the differentiation of multi-scale features and the accuracy of recognition.It is found that augmenting the image's detail capacity does not always yield benefits for vision-based recognition models.In light of these observations,we propose a scale hybridization approach based on the diffusion mechanism of scale-space representation.The results show that scale hybridization strengthens the tolerance of multi-scale feature recognition under complex environmental noise interference and significantly enhances the recognition accuracy of GD.It also facilitates the objective understanding,description and analysis of the rock behavior and stability of slopes from the perspective of image data.展开更多
DOG1(Delay of germination 1)蛋白是种子休眠的一种主要调控因子,可与ABA协同作用来延迟种子萌发。核心脱落酸(ABA)信号转导途径和DOG1途径在蛋白磷酸酶2C(PP2C)中汇聚。DOG1调控种子休眠需要PP2C,并通过与ABA过敏感萌发(AHG1/AHG3)结...DOG1(Delay of germination 1)蛋白是种子休眠的一种主要调控因子,可与ABA协同作用来延迟种子萌发。核心脱落酸(ABA)信号转导途径和DOG1途径在蛋白磷酸酶2C(PP2C)中汇聚。DOG1调控种子休眠需要PP2C,并通过与ABA过敏感萌发(AHG1/AHG3)结合来增强ABA的信号转导。DOG1抑制AHG1的作用来增加ABA的敏感性和诱导种子休眠。近年来,DOG1对种子休眠与萌发的调控研究取得了显著进展。本文对该领域的研究成果进行了综述,主要包括:DOG1对种子成熟、休眠与萌发的作用,DOG1表达的转录调控机制以及DOG1对种子休眠与萌发的调控及作用机制,最后提出了在本领域需要进一步研究的科学问题。展开更多
An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dyna...An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dynamical and sustained authentication. This paper proposes a basic function of constructing new scale-spaces with deep detecting ability and high stability for image features aimed at image root generation. According to the heat distribution and spreading principle of various kinds of infinitesimal heat sources in the space medium,a multi-embed nonlinear diffusion equation that corresponds to the multi-embed nonlinear scale-space is proposed,a HARRIS-HESSIAN scale-space evaluation operator that aims at the structure acceleration characteristics of a local region and can make use of image pixels' relative spreading movement principle was constructed,then a single-parameter global symmetric proportion(SPGSP) operator was also constructed. An authentication test with 3000 to 5000 cloud entities shows the new scale-space can work well and is stable,when the whole cloud has 5%-50% behavior with un-trusted entities. Consequently,it can be used as the corresponding stable logic feature generation model and algorithm for all kinds of images,and logic relationships among image features for trust roots.展开更多
Retroviruses have been proven to cause infections and diseases in a series of mammalian hosts but not in dogs.Then,this letter discussed the dog susceptibility to retrovirus infection,encompassing arguments to underst...Retroviruses have been proven to cause infections and diseases in a series of mammalian hosts but not in dogs.Then,this letter discussed the dog susceptibility to retrovirus infection,encompassing arguments to understand why dogs may have not been infected by retroviruses thus far.The potential resistance of retrovirus in dogs enables this provocative short communication to discuss this question,looking at some evolutive aspects.The lineage of canids has shown,throughout its evolutionary history,a smaller accumulation of retroviruses in canid genomes,classifed as endogenous retroviruses.In this context,the genomes of canids seem to ofer obstacles,which have been evolutionarily conserved,in the face of retroviral infection.展开更多
Chronic kidney disease is commonly diagnosed in dogs,and clinical signs may be aggravated when infected agents are involved.In this case report,33 dogs with chronic kidney disease were clinically evaluated and serolog...Chronic kidney disease is commonly diagnosed in dogs,and clinical signs may be aggravated when infected agents are involved.In this case report,33 dogs with chronic kidney disease were clinically evaluated and serologically tested for Leptospira spp.,Ehrlichia canis,and Anaplasma phagocytophilum.The seroprevalence for Leptospira spp.was 39.4%.The most frequent serovars found were Pyrogenes,Canicola,Bratislava and Australis,with serological titers between 1:100 to 1:800.Clinical signs included fever,depression,decreased body condition,vomiting and hema‑turia.Signifcant laboratory fndings were anemia,leukocytosis,thrombocytopenia,increased liver enzymes,urea and creatinine,hyperbilirubinemia and hyperphosphatemia.All leptospira seronegative dogs were positive for one or both monitored homoparasites(i.e.,E.canis and A.phagocytophilum);only three leptospira seropositive dogs were positive for one or both hemoparasites.Findings also suggest that endemic hemoparasites of dogs should be moni‑tored in dogs with a kidney condition for a better clinical picture of the patients and therapeutic approach.展开更多
The aim of the present study was to compare the ixodicide efficacy of 1% permethrin applied as a shampoo-bath in single treatment and a combined formulation of 2% permethrin + 1% piperonyl butoxide applied as powder i...The aim of the present study was to compare the ixodicide efficacy of 1% permethrin applied as a shampoo-bath in single treatment and a combined formulation of 2% permethrin + 1% piperonyl butoxide applied as powder in a single treatment against Rhipicephalus sanguineus ticks, in naturally infested dogs. Eighteen adult cross-mixed dogs, harboring to at least 20 ticks/animal, were selected on pre-treatment live tick counts. On day 0, the dogs were then divided in 3 groups of 6 animals each, for treatment: Group 1 (G1) received 1% permethrin applied as shampoo-bath (approximately 10 ml/kg bw), according to label instructions. G2 was treated with 2% permethrin + 1% piperonyl butoxide applied as powder, and rubbed as massage on the body, as recommended by the producer. G3 served as a non-treated control. After the treatment, the dogs were checked out for live ticks counting on days 0, 1, 7, and 14. The ixodicide efficacy was assessed based on the percentage reductions of ticks on the days analyzed post-treatment, with respect to the untreated control. Results showed that permethrin alone removed ticks on 81%, 93.1% and 89.6% for days 1, 7 and 14, respectively. The combined formulation of 2% permethrin + 1% piperonyl butoxide exerted a better efficacy of 98.8, 97.7 and 97.0%, respectively. It is concluded that this combined formulation was highly effective at rapidly repelling and killing Riphicephalus sanguineus ticks on naturally infected dogs, showing a significant effect on days 1, 7 and 14 after tick exposure. The sustained high and quick level of efficacy of this combination may well interfere with the transmission of Riphicephalus sanguineus ticks. It is concluded that the sustained high level of efficacy of the permethrin + piperonyl butoxide combination may well exert an additive effect and could interfere with the transmission of Riphicephalus sanguineus ticks.展开更多
Canine parvovirus 2(CPV-2)is a highly contagious virus in dogs that typically causes hemorrhagic enteritis and a high mortality rate in unvaccinated puppies.The genetic variability and antigenic diversity of CPV-2 hin...Canine parvovirus 2(CPV-2)is a highly contagious virus in dogs that typically causes hemorrhagic enteritis and a high mortality rate in unvaccinated puppies.The genetic variability and antigenic diversity of CPV-2 hinder its efective prevention of infection by vaccination.To investigate the epidemiology and genetic characteristics of CPV-2 in China,rectal swabs from afected dogs were collected from diferent animal clinics in Kunshan from 2022 to 2023.Preliminary detection and capsid gene sequencing of CPV-2 were performed using previously described primers and protocols.The overall detection rate for CPV-2 was 16.5%(33/200).A signifcant association was found between the CPV-2-positivity and clinical signs,age,breed and vaccination status.Sequence analysis revealed the presence of CPV-2c genotypes in all positive samples,which were genetically similar to other Asian CPV-2c strains.Notably,four key mutations(A5G,F267Y,Y324I and Q370R)were detected in all isolates,and one novel mutation(I447M)was detected in three CPV-2 isolates.These mutations in the CPV-2 strains could impact vaccine efcacy and the efectiveness of the virus immune evasion.Surprisingly,no recombination events were observed between the identifed CPV-2c strains and reference strains from China.Our data revealed that amino acid residues 324,426 and 440 of VP2 may under strong selection pressure.This pattern of genetic variation in the CPV-2 lineage warrants continuous laboratory-based surveillance programs in other parts of China to better understand the pattern of seasonal distribution and association between emerging genotypes and the intensity of disease severity.展开更多
Feline panleukopenia virus(FPV)is a single-stranded DNA virus that can infect cats and cause feline panleukopenia,which is a highly contagious and fatal disease in felines.The sequence of FPV is highly variable,and mu...Feline panleukopenia virus(FPV)is a single-stranded DNA virus that can infect cats and cause feline panleukopenia,which is a highly contagious and fatal disease in felines.The sequence of FPV is highly variable,and mutations in the amino acids of its capsid protein play crucial roles in altering viral virulence,immunogenicity,host selection,and other abilities.In this study,the epidemiology of FPV was studied using 746 gastrointestinal swab samples derived from cats that presented gastrointestinal symptoms specifcally,diarrhea or vomiting during the period spanning from 2018 to 2022.The overall prevalence of FPV-positive patients among these samples was determined to be 45.4%.Capsid(virion)protein 2(VP2)gene of each FPV-positive sample was sequenced and amplifed,yielding 65 VP2 sequences.Among them,six VP2 gene sequences were detected in the majority of the samples test positive for FPV,and these positive samples originated from a diverse range of geographical locations.These isolates were named FPV-6,FPV-10,FPV-15,FPV-251,FPV-271 and FPV-S2.Additionally,the substitution of Ala300Pro(A300P)in VP2 was detected for the frst time in feline-derived FPV(FPV-251).FPV-251 isolate,with this substitution in VP2 protein,exhibited stable proliferative capacity in Madin-Darby canine kidney(MDCK)cells and A72 cells.FPV-271 was selected as the FPV control isolate due to its single amino acid diference from VP2 protein of FPV-251 at position 300(FPV-271 has alanine,while FPV-251 has proline).After oral infection,both FPV-251 and FPV-271 isolates caused feline panleukopenia,which is characterized by clinical signs of enterocolitis.However,FPV-251 can infect dogs through the oral route and cause gastrointestinal(GI)symptoms with lesions in the intestine and mesenteric lymph nodes(MLNs)of infected dogs.This is the frst report on the presence of an A300P substitution in VP2 protein of feline-derived FPV.Additionally,FPV isolate with a substitution of A300P at VP2 protein demonstrated efcient replication capabilities in canine cell lines and the ability to infect dogs.展开更多
Every year, a higher number of dogs are abandoned or euthanised due to temperament issues and a lack of understanding by owners regarding dog behaviour and training. This research focuses on the potential to make pred...Every year, a higher number of dogs are abandoned or euthanised due to temperament issues and a lack of understanding by owners regarding dog behaviour and training. This research focuses on the potential to make predictions of adult dog temperament based on early puppy behaviours by using a machine learning model. Specifically, the research used guard dog breeds, such as American Bully, American Pit Bull Terrier, and German Shepherd. The study collected dog data and general data from dog owners and used the Random Forest approach to build a predictive model. Users are allowed to input puppy data and receive adult dog temperament predictions in model, which is integrated into a web application. The aims of this web application are to enhance responsible dog ownership and reduce abandonment by offering insights and training recommendations based on predicted outcomes. The model achieved a prediction accuracy of 86% on testing, and it is continually improving, though further refinement is recommended to improve its reliability and applicability across a broader range of breeds. The study contributes to canine welfare by providing a practical solution for predicting temperament outcomes, ultimately helping to reduce shelter populations and euthanasia rates.展开更多
Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding spe...Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding specific topical protocols is still scarce. This study aimed to evaluate the performance of a protocol combining chlorhexidine and Ophytrium-containing products in reducing yeast counts in dogs with microscopic evidence of yeast imbalance and dermatological signs. Dogs were shampooed on Day 0 (D0) and then received eight mousse applications at two to three-day intervals. Followed-up parameters on D0, Day 7 (D7), and Day 21 (D21) were microscopic yeast counting, pruritus, and skin irritation scores. At D21, veterinarians’ and owners’ overall assessments were collected. Malassezia pachydermatis counts decreased at D7 (57.3% vs D0, p p p p < 0.001). Efficacy was described by all veterinarians from good to excellent, and the proposed procedures were evaluated as efficient and practical by the owners in 91.7% of the cases. The protocol using chlorhexidine and Ophytrium shampoo and mousse could be a useful option in dogs presenting imbalances in yeast cutaneous microbiota.展开更多
Peter Heller’s The Dog Stars is a fascinating meditation of post-apocalyptic loneliness.Set in a dismal world devastated by a devastating virus,the story centers on the protagonist Hig’s experience and response as h...Peter Heller’s The Dog Stars is a fascinating meditation of post-apocalyptic loneliness.Set in a dismal world devastated by a devastating virus,the story centers on the protagonist Hig’s experience and response as he navigates a terrain devoid of human connection and meaning.This paper investigates the complexities of loneliness,focusing on Hig’s response to the loss of his wife and dog.Furthermore,it delves into the transformational power of companionship,including Hig’s link with his dog Jasper and his ultimate connection with Cima and her father.The fragmented narrative style of the novel mirrors Hig’s internal struggle,offering a raw and intimate portrayal of his journey towards reconnection and hope.Through the novel,Heller offers his profound thinking on the significance of connection in overcoming even post-apocalyptic loneliness.展开更多
Activity 1.Think about the following questions and write down your answers before reading the text.1.Do you know some foods are toxic to dogs?2.What foods do you avoid giving your dog?
The cloud computing technology is utilized for achieving resource utilization of remotebased virtual computer to facilitate the consumers with rapid and accurate massive data services.It utilizes on-demand resource pr...The cloud computing technology is utilized for achieving resource utilization of remotebased virtual computer to facilitate the consumers with rapid and accurate massive data services.It utilizes on-demand resource provisioning,but the necessitated constraints of rapid turnaround time,minimal execution cost,high rate of resource utilization and limited makespan transforms the Load Balancing(LB)process-based Task Scheduling(TS)problem into an NP-hard optimization issue.In this paper,Hybrid Prairie Dog and Beluga Whale Optimization Algorithm(HPDBWOA)is propounded for precise mapping of tasks to virtual machines with the due objective of addressing the dynamic nature of cloud environment.This capability of HPDBWOA helps in decreasing the SLA violations and Makespan with optimal resource management.It is modelled as a scheduling strategy which utilizes the merits of PDOA and BWOA for attaining reactive decisions making with respect to the process of assigning the tasks to virtual resources by considering their priorities into account.It addresses the problem of pre-convergence with wellbalanced exploration and exploitation to attain necessitated Quality of Service(QoS)for minimizing the waiting time incurred during TS process.It further balanced exploration and exploitation rates for reducing the makespan during the task allocation with complete awareness of VM state.The results of the proposed HPDBWOA confirmed minimized energy utilization of 32.18% and reduced cost of 28.94% better than approaches used for investigation.The statistical investigation of the proposed HPDBWOA conducted using ANOVA confirmed its efficacy over the benchmarked systems in terms of throughput,system,and response time.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.52090081)the State Key Laboratory of Hydro-science and Hydraulic Engineering(Grant No.2021-KY-04).
文摘Geological discontinuity(GD)plays a pivotal role in determining the catastrophic mechanical failure of jointed rock masses.Accurate and efficient acquisition of GD networks is essential for characterizing and understanding the progressive damage mechanisms of slopes based on monitoring image data.Inspired by recent advances in computer vision,deep learning(DL)models have been widely utilized for image-based fracture identification.The multi-scale characteristics,image resolution and annotation quality of images will cause a scale-space effect(SSE)that makes features indistinguishable from noise,directly affecting the accuracy.However,this effect has not received adequate attention.Herein,we try to address this gap by collecting slope images at various proportional scales and constructing multi-scale datasets using image processing techniques.Next,we quantify the intensity of feature signals using metrics such as peak signal-to-noise ratio(PSNR)and structural similarity(SSIM).Combining these metrics with the scale-space theory,we investigate the influence of the SSE on the differentiation of multi-scale features and the accuracy of recognition.It is found that augmenting the image's detail capacity does not always yield benefits for vision-based recognition models.In light of these observations,we propose a scale hybridization approach based on the diffusion mechanism of scale-space representation.The results show that scale hybridization strengthens the tolerance of multi-scale feature recognition under complex environmental noise interference and significantly enhances the recognition accuracy of GD.It also facilitates the objective understanding,description and analysis of the rock behavior and stability of slopes from the perspective of image data.
文摘DOG1(Delay of germination 1)蛋白是种子休眠的一种主要调控因子,可与ABA协同作用来延迟种子萌发。核心脱落酸(ABA)信号转导途径和DOG1途径在蛋白磷酸酶2C(PP2C)中汇聚。DOG1调控种子休眠需要PP2C,并通过与ABA过敏感萌发(AHG1/AHG3)结合来增强ABA的信号转导。DOG1抑制AHG1的作用来增加ABA的敏感性和诱导种子休眠。近年来,DOG1对种子休眠与萌发的调控研究取得了显著进展。本文对该领域的研究成果进行了综述,主要包括:DOG1对种子成熟、休眠与萌发的作用,DOG1表达的转录调控机制以及DOG1对种子休眠与萌发的调控及作用机制,最后提出了在本领域需要进一步研究的科学问题。
基金The national natural science foundation (61672442,61503316,61273290,61373147)Xiamen Scientific Plan Project (2014S0048,3502Z20123037)+1 种基金Fujian Scientific Plan Project (2013HZ00041)Fujian provincial education office A-class project(JA13238)
文摘An image trust root is a special type of soft trust root for trusted computing. However,image trust root generation is difficult,as it needs a corresponding stable logic feature generation model and algorithm for dynamical and sustained authentication. This paper proposes a basic function of constructing new scale-spaces with deep detecting ability and high stability for image features aimed at image root generation. According to the heat distribution and spreading principle of various kinds of infinitesimal heat sources in the space medium,a multi-embed nonlinear diffusion equation that corresponds to the multi-embed nonlinear scale-space is proposed,a HARRIS-HESSIAN scale-space evaluation operator that aims at the structure acceleration characteristics of a local region and can make use of image pixels' relative spreading movement principle was constructed,then a single-parameter global symmetric proportion(SPGSP) operator was also constructed. An authentication test with 3000 to 5000 cloud entities shows the new scale-space can work well and is stable,when the whole cloud has 5%-50% behavior with un-trusted entities. Consequently,it can be used as the corresponding stable logic feature generation model and algorithm for all kinds of images,and logic relationships among image features for trust roots.
基金Fapesp 2014/22827–7Ministério da Saúde do BrasilFundação Faculdade de Medicina and CNPq Grant to JC:301275/2019–0.
文摘Retroviruses have been proven to cause infections and diseases in a series of mammalian hosts but not in dogs.Then,this letter discussed the dog susceptibility to retrovirus infection,encompassing arguments to understand why dogs may have not been infected by retroviruses thus far.The potential resistance of retrovirus in dogs enables this provocative short communication to discuss this question,looking at some evolutive aspects.The lineage of canids has shown,throughout its evolutionary history,a smaller accumulation of retroviruses in canid genomes,classifed as endogenous retroviruses.In this context,the genomes of canids seem to ofer obstacles,which have been evolutionarily conserved,in the face of retroviral infection.
文摘Chronic kidney disease is commonly diagnosed in dogs,and clinical signs may be aggravated when infected agents are involved.In this case report,33 dogs with chronic kidney disease were clinically evaluated and serologically tested for Leptospira spp.,Ehrlichia canis,and Anaplasma phagocytophilum.The seroprevalence for Leptospira spp.was 39.4%.The most frequent serovars found were Pyrogenes,Canicola,Bratislava and Australis,with serological titers between 1:100 to 1:800.Clinical signs included fever,depression,decreased body condition,vomiting and hema‑turia.Signifcant laboratory fndings were anemia,leukocytosis,thrombocytopenia,increased liver enzymes,urea and creatinine,hyperbilirubinemia and hyperphosphatemia.All leptospira seronegative dogs were positive for one or both monitored homoparasites(i.e.,E.canis and A.phagocytophilum);only three leptospira seropositive dogs were positive for one or both hemoparasites.Findings also suggest that endemic hemoparasites of dogs should be moni‑tored in dogs with a kidney condition for a better clinical picture of the patients and therapeutic approach.
文摘The aim of the present study was to compare the ixodicide efficacy of 1% permethrin applied as a shampoo-bath in single treatment and a combined formulation of 2% permethrin + 1% piperonyl butoxide applied as powder in a single treatment against Rhipicephalus sanguineus ticks, in naturally infested dogs. Eighteen adult cross-mixed dogs, harboring to at least 20 ticks/animal, were selected on pre-treatment live tick counts. On day 0, the dogs were then divided in 3 groups of 6 animals each, for treatment: Group 1 (G1) received 1% permethrin applied as shampoo-bath (approximately 10 ml/kg bw), according to label instructions. G2 was treated with 2% permethrin + 1% piperonyl butoxide applied as powder, and rubbed as massage on the body, as recommended by the producer. G3 served as a non-treated control. After the treatment, the dogs were checked out for live ticks counting on days 0, 1, 7, and 14. The ixodicide efficacy was assessed based on the percentage reductions of ticks on the days analyzed post-treatment, with respect to the untreated control. Results showed that permethrin alone removed ticks on 81%, 93.1% and 89.6% for days 1, 7 and 14, respectively. The combined formulation of 2% permethrin + 1% piperonyl butoxide exerted a better efficacy of 98.8, 97.7 and 97.0%, respectively. It is concluded that this combined formulation was highly effective at rapidly repelling and killing Riphicephalus sanguineus ticks on naturally infected dogs, showing a significant effect on days 1, 7 and 14 after tick exposure. The sustained high and quick level of efficacy of this combination may well interfere with the transmission of Riphicephalus sanguineus ticks. It is concluded that the sustained high level of efficacy of the permethrin + piperonyl butoxide combination may well exert an additive effect and could interfere with the transmission of Riphicephalus sanguineus ticks.
基金Kunshan Municipal Government research funding(Grant Number:22KKSGR075).
文摘Canine parvovirus 2(CPV-2)is a highly contagious virus in dogs that typically causes hemorrhagic enteritis and a high mortality rate in unvaccinated puppies.The genetic variability and antigenic diversity of CPV-2 hinder its efective prevention of infection by vaccination.To investigate the epidemiology and genetic characteristics of CPV-2 in China,rectal swabs from afected dogs were collected from diferent animal clinics in Kunshan from 2022 to 2023.Preliminary detection and capsid gene sequencing of CPV-2 were performed using previously described primers and protocols.The overall detection rate for CPV-2 was 16.5%(33/200).A signifcant association was found between the CPV-2-positivity and clinical signs,age,breed and vaccination status.Sequence analysis revealed the presence of CPV-2c genotypes in all positive samples,which were genetically similar to other Asian CPV-2c strains.Notably,four key mutations(A5G,F267Y,Y324I and Q370R)were detected in all isolates,and one novel mutation(I447M)was detected in three CPV-2 isolates.These mutations in the CPV-2 strains could impact vaccine efcacy and the efectiveness of the virus immune evasion.Surprisingly,no recombination events were observed between the identifed CPV-2c strains and reference strains from China.Our data revealed that amino acid residues 324,426 and 440 of VP2 may under strong selection pressure.This pattern of genetic variation in the CPV-2 lineage warrants continuous laboratory-based surveillance programs in other parts of China to better understand the pattern of seasonal distribution and association between emerging genotypes and the intensity of disease severity.
基金the Experimental Animal Research Project of Hubei Province(Grant No.2023CFA005).
文摘Feline panleukopenia virus(FPV)is a single-stranded DNA virus that can infect cats and cause feline panleukopenia,which is a highly contagious and fatal disease in felines.The sequence of FPV is highly variable,and mutations in the amino acids of its capsid protein play crucial roles in altering viral virulence,immunogenicity,host selection,and other abilities.In this study,the epidemiology of FPV was studied using 746 gastrointestinal swab samples derived from cats that presented gastrointestinal symptoms specifcally,diarrhea or vomiting during the period spanning from 2018 to 2022.The overall prevalence of FPV-positive patients among these samples was determined to be 45.4%.Capsid(virion)protein 2(VP2)gene of each FPV-positive sample was sequenced and amplifed,yielding 65 VP2 sequences.Among them,six VP2 gene sequences were detected in the majority of the samples test positive for FPV,and these positive samples originated from a diverse range of geographical locations.These isolates were named FPV-6,FPV-10,FPV-15,FPV-251,FPV-271 and FPV-S2.Additionally,the substitution of Ala300Pro(A300P)in VP2 was detected for the frst time in feline-derived FPV(FPV-251).FPV-251 isolate,with this substitution in VP2 protein,exhibited stable proliferative capacity in Madin-Darby canine kidney(MDCK)cells and A72 cells.FPV-271 was selected as the FPV control isolate due to its single amino acid diference from VP2 protein of FPV-251 at position 300(FPV-271 has alanine,while FPV-251 has proline).After oral infection,both FPV-251 and FPV-271 isolates caused feline panleukopenia,which is characterized by clinical signs of enterocolitis.However,FPV-251 can infect dogs through the oral route and cause gastrointestinal(GI)symptoms with lesions in the intestine and mesenteric lymph nodes(MLNs)of infected dogs.This is the frst report on the presence of an A300P substitution in VP2 protein of feline-derived FPV.Additionally,FPV isolate with a substitution of A300P at VP2 protein demonstrated efcient replication capabilities in canine cell lines and the ability to infect dogs.
文摘Every year, a higher number of dogs are abandoned or euthanised due to temperament issues and a lack of understanding by owners regarding dog behaviour and training. This research focuses on the potential to make predictions of adult dog temperament based on early puppy behaviours by using a machine learning model. Specifically, the research used guard dog breeds, such as American Bully, American Pit Bull Terrier, and German Shepherd. The study collected dog data and general data from dog owners and used the Random Forest approach to build a predictive model. Users are allowed to input puppy data and receive adult dog temperament predictions in model, which is integrated into a web application. The aims of this web application are to enhance responsible dog ownership and reduce abandonment by offering insights and training recommendations based on predicted outcomes. The model achieved a prediction accuracy of 86% on testing, and it is continually improving, though further refinement is recommended to improve its reliability and applicability across a broader range of breeds. The study contributes to canine welfare by providing a practical solution for predicting temperament outcomes, ultimately helping to reduce shelter populations and euthanasia rates.
文摘Superficial microbiota disturbances are common in dogs, with Malassezia pachydermatis yeasts often being involved. Topical products are recommended as a first-line management strategy;however, literature regarding specific topical protocols is still scarce. This study aimed to evaluate the performance of a protocol combining chlorhexidine and Ophytrium-containing products in reducing yeast counts in dogs with microscopic evidence of yeast imbalance and dermatological signs. Dogs were shampooed on Day 0 (D0) and then received eight mousse applications at two to three-day intervals. Followed-up parameters on D0, Day 7 (D7), and Day 21 (D21) were microscopic yeast counting, pruritus, and skin irritation scores. At D21, veterinarians’ and owners’ overall assessments were collected. Malassezia pachydermatis counts decreased at D7 (57.3% vs D0, p p p p < 0.001). Efficacy was described by all veterinarians from good to excellent, and the proposed procedures were evaluated as efficient and practical by the owners in 91.7% of the cases. The protocol using chlorhexidine and Ophytrium shampoo and mousse could be a useful option in dogs presenting imbalances in yeast cutaneous microbiota.
基金sponsored by the Jiangsu Social Science Fund under the grant title“A Study of Post-apocalyptic Writing in Contemporary American Novels”(20WWB005)supported by the Fundamental Research Funds for the Central Universities,No.XAB22018.
文摘Peter Heller’s The Dog Stars is a fascinating meditation of post-apocalyptic loneliness.Set in a dismal world devastated by a devastating virus,the story centers on the protagonist Hig’s experience and response as he navigates a terrain devoid of human connection and meaning.This paper investigates the complexities of loneliness,focusing on Hig’s response to the loss of his wife and dog.Furthermore,it delves into the transformational power of companionship,including Hig’s link with his dog Jasper and his ultimate connection with Cima and her father.The fragmented narrative style of the novel mirrors Hig’s internal struggle,offering a raw and intimate portrayal of his journey towards reconnection and hope.Through the novel,Heller offers his profound thinking on the significance of connection in overcoming even post-apocalyptic loneliness.
文摘Activity 1.Think about the following questions and write down your answers before reading the text.1.Do you know some foods are toxic to dogs?2.What foods do you avoid giving your dog?
文摘The cloud computing technology is utilized for achieving resource utilization of remotebased virtual computer to facilitate the consumers with rapid and accurate massive data services.It utilizes on-demand resource provisioning,but the necessitated constraints of rapid turnaround time,minimal execution cost,high rate of resource utilization and limited makespan transforms the Load Balancing(LB)process-based Task Scheduling(TS)problem into an NP-hard optimization issue.In this paper,Hybrid Prairie Dog and Beluga Whale Optimization Algorithm(HPDBWOA)is propounded for precise mapping of tasks to virtual machines with the due objective of addressing the dynamic nature of cloud environment.This capability of HPDBWOA helps in decreasing the SLA violations and Makespan with optimal resource management.It is modelled as a scheduling strategy which utilizes the merits of PDOA and BWOA for attaining reactive decisions making with respect to the process of assigning the tasks to virtual resources by considering their priorities into account.It addresses the problem of pre-convergence with wellbalanced exploration and exploitation to attain necessitated Quality of Service(QoS)for minimizing the waiting time incurred during TS process.It further balanced exploration and exploitation rates for reducing the makespan during the task allocation with complete awareness of VM state.The results of the proposed HPDBWOA confirmed minimized energy utilization of 32.18% and reduced cost of 28.94% better than approaches used for investigation.The statistical investigation of the proposed HPDBWOA conducted using ANOVA confirmed its efficacy over the benchmarked systems in terms of throughput,system,and response time.
