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On Several Shining Points of Lin Daiyu’s Personality Praits in A Dream of Red Mansions
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作者 居丽萍 《海外英语》 2020年第21期227-228,共2页
This paper,from five different facets,outlines several strong highlights from the heroine Lin Daiyu’s main character through reading a Chinese classic fantastic novel A Dream of Red Mansions,who otherwise is always c... This paper,from five different facets,outlines several strong highlights from the heroine Lin Daiyu’s main character through reading a Chinese classic fantastic novel A Dream of Red Mansions,who otherwise is always considered as a weak,maver⁃ick,delicate and sentimental girl by many Chinese common readers. 展开更多
关键词 HIGHLIGHTs Lin Daiyu’s Character A Dream of Red mansions
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贡山三尖杉的新名称(Cephalotaxus talonensis Cheng et Feng ex S.G.Lu et X.D.Lang)及分类地位(英文) 被引量:1
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作者 郎学东 苏建荣 陆树刚 《植物研究》 CAS CSCD 北大核心 2013年第1期4-6,共3页
提议贡山三尖杉的新名称Cephalotaxus talonensis Cheng et Feng ex S.G.Lu et X.D.Lang替代不合法的旧名称Cephalotaxus lanceolata K.M.Feng in Cheng et al.(晚出同名)。对比贡山三尖杉和三尖杉Cephalotaxus fortunei Hooker的模式... 提议贡山三尖杉的新名称Cephalotaxus talonensis Cheng et Feng ex S.G.Lu et X.D.Lang替代不合法的旧名称Cephalotaxus lanceolata K.M.Feng in Cheng et al.(晚出同名)。对比贡山三尖杉和三尖杉Cephalotaxus fortunei Hooker的模式标本和自然生长状态下的叶片形态,支持将贡山三尖杉处理为种的等级,不赞同Silba(1990)将贡山三尖杉降为三尖杉的变种[Cephalotaxus fortunei Hooker var.lanceolata(Feng)Silba]。文中提供4幅参照图片。 展开更多
关键词 三尖杉科 Cephalotaxus talonensis cheng ET Feng EX s G LU ET X D Lang 新名称
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A Memoir of Beijing Opera Performers: Cheng Yanqiu’s Early Life and His Art
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作者 ZHANG Yi-he LIU Yue 《Journal of Literature and Art Studies》 2021年第12期1034-1040,共7页
As one of the greatest m asters of Dan 1 role performers in Beijing Opera history,Cheng Yanqiu had a very hard time in his childhood in learning the art.In this article,the author has reviewed his early life and probe... As one of the greatest m asters of Dan 1 role performers in Beijing Opera history,Cheng Yanqiu had a very hard time in his childhood in learning the art.In this article,the author has reviewed his early life and probed into the help of Luo Yinggong who supported for his great su ccess.The author has also explored the art of Cheng’s singing,his study from Wang Yaoqing and explored how he created the melodies as well as trying to solve his making-up mystery. 展开更多
关键词 Beijing Opera performer Dan role chengs school art
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Visiting the Garden at Prince Gong 's Mansion
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作者 Li Xiao Xia Youhao 《China & The World Cultural Exchange》 2000年第5期26-28,共3页
关键词 s mansion Visiting the Garden at Prince Gong
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A Comparative Study of the Poem Translation Styles of the German Translation of A Dream of Red Mansions From the Perspective of Corpus-Translatology
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作者 ZHAO Xuan 《Cultural and Religious Studies》 2022年第8期448-453,共6页
Poetry is an integral part of the artistic framework of A Dream of Red Mansions,and plays a crucial role in shaping the atmosphere,portraying characters,and reflecting social reality,therefore it has both unique artis... Poetry is an integral part of the artistic framework of A Dream of Red Mansions,and plays a crucial role in shaping the atmosphere,portraying characters,and reflecting social reality,therefore it has both unique artistic charm and research value.This paper takes Kuhn’s and Schwarz’s translation of A Dream of Red Mansions as the research objects,selects 19 poems that both translators have translated to make a corpus,uses the software LancsBox to compare the translations from three levels of vocabulary,syntax,and text,in order to provide a new interpretation dimension for the translation research of A Dream of Red Mansions and enrich the application of the corpus-based method in the field of translation research of Chinese classics. 展开更多
关键词 corpus-based translation studies A Dream of Red mansions poetry translation translator’s style
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霍山石斛多糖通过激活GSK-3β信号通路减轻大鼠心肌缺血-再灌注损伤 被引量:9
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作者 房雪 韩吉春 +2 位作者 李德芳 王凤华 郑秋生 《中药材》 CAS 北大核心 2017年第4期925-930,共6页
目的:研究霍山石斛多糖抗心肌缺血-再灌注损伤的作用。方法:采用Langendorff离体灌流系统建造大鼠离体缺血-再灌注损伤模型。实验分为正常对照组(不停灌处理)、模型组(停灌20 min)及霍山石斛多糖低、中、高浓度(停灌前灌流已配制浓度为... 目的:研究霍山石斛多糖抗心肌缺血-再灌注损伤的作用。方法:采用Langendorff离体灌流系统建造大鼠离体缺血-再灌注损伤模型。实验分为正常对照组(不停灌处理)、模型组(停灌20 min)及霍山石斛多糖低、中、高浓度(停灌前灌流已配制浓度为0.1、0.5、1.0 mg/L的KH液10 min)组。利用生物多导记录仪连续测定血流动力学参数。通过TTC染色检测心肌梗死面积。并用ELISA法检测冠脉流出液中肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性。ELISA法检测心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)比率、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的含量。利用HE染色和TUNEL法分别检测心肌细胞的坏死和凋亡。Western blot检测心肌组织中GSK-3β的磷酸化水平。结果:与正常对照组比较,模型组出现严重的心肌坏死和极高的凋亡率,心功能下降,冠脉流出液中LDH和CK水平显著升高,心肌组织中MDA、IL-6和TNF-α含量显著升高,SOD的活性与GSH/GSSG比值均显著降低(P<0.01)。与模型组比较,霍山石斛多糖中、高剂量组的心肌坏死程度、梗死面积和细胞凋亡率显著降低,心功能显著改善;冠脉流出液中LDH、CK水平逐渐降低;心肌组织中MDA、TNF-α及IL-6含量显著降低,SOD活性和GSH/GSSG比值显著升高(P<0.01)。高剂量霍山石斛多糖可显著降低心肌细胞凋亡指数,提高心肌组织中GSK-3β磷酸化水平。结论:霍山石斛多糖具有显著的抗心肌缺血-再灌注损伤作用,其作用机制可能与激活GSK-3β信号通路发挥抗氧化、抗炎和抗凋亡有关。 展开更多
关键词 霍山石斛多糖 心脏保护 抗氧化 抗炎 抗凋亡 GsK-3Β
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马鸿斌主任医师运用程氏萆薢分清饮治疗慢性肾炎临证探析
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作者 黄锦蕊 常育博 +1 位作者 马鸿斌 魏锦慧 《亚太传统医药》 2025年第1期79-83,共5页
慢性肾炎是临床常见肾系疾病,病因复杂,病理类型多样,根据其蛋白尿、血尿、水肿等临床表现,大致属于中医“淋证、水肿、尿浊”的范畴,马鸿斌主任医师指出慢性肾炎病机为风、湿、热、瘀、浊五邪交织为患,脾胃肾两脏一腑虚衰,导致水液通... 慢性肾炎是临床常见肾系疾病,病因复杂,病理类型多样,根据其蛋白尿、血尿、水肿等临床表现,大致属于中医“淋证、水肿、尿浊”的范畴,马鸿斌主任医师指出慢性肾炎病机为风、湿、热、瘀、浊五邪交织为患,脾胃肾两脏一腑虚衰,导致水液通调失常,早期以祛风补虚为主,后期湿、热、瘀血流连肾脏,须清热利湿、活血祛瘀。运用程氏萆薢分清饮加减治疗慢性肾炎,以清利下焦湿热兼活血化瘀,平补肾中阴阳为原则,通过抑制炎症反应、调节肠道菌群、促进尿酸排泄、改善肾纤维化程度等,减轻患者症状,在临床效如桴鼓。 展开更多
关键词 慢性肾炎 水肿 湿热 程氏萆薢分清饮
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Treatment of abdominal compartment syndrome in severe acute pancreatitis patients with traditional Chinese medicine 被引量:35
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作者 Min-Jie Zhang Guo-Lei Zhang +2 位作者 Wen-Bin Yuan Jun Ni Li-Feng Huang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第22期3574-3578,共5页
AIM: To investigate the therapeutic effect of traditional Chinese traditional medicines Da Cheng Qi Decoction (Timely-Purging and Yin-Preserving Decoction) and Glauber's salt combined with conservative measures on... AIM: To investigate the therapeutic effect of traditional Chinese traditional medicines Da Cheng Qi Decoction (Timely-Purging and Yin-Preserving Decoction) and Glauber's salt combined with conservative measures on abdominal compartment syndrome (ACS) in severe acute pancreatitis (SAP) patients. METHODS: Eighty consecutive SAP patients, admitted for routine non-operative conservative treatment, were randomly divided into study group and control group (40 patients in each group). Patients in the study group received Da Cheng Qi Decoction enema for 2 h and external use of Glauber's salt, once a day for 7 d. Patients in the control group received normal saline (NS) enema. Routine non-operative conservative treatments included non-per os nutrition (NPON), gastrointestinal decompression, life support, total parenteral nutrition (TPN), continuous peripancreatic vascular pharmaceutical infusion and drug therapy. Intra-cystic pressure (ICP) of the two groups was measured during treatment. The effectiveness and outcomes of treatment were observed and APACHE Ⅱ scores were applied in analysis. RESULTS: On days 4 and 5 of treatment, the ICP was lower in the study group than in the control group(P < 0.05). On days 3-5 of treatment, acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) scores for the study and control groups were significantly different (P < 0.05). Both the effectiveness and outcome of the treatment with Da Cheng Qi Decoction on abdominalgia, burbulence relief time, ascites quantity, cyst formation rate and hospitalization time were quite different between the two groups (P < 0.05). The mortality rate for the two groups had no significant difference. CONCLUSION: Da Cheng Qi Decoction enema and external use of Glauber's salt combined with routine non-operative conservative treatment can decrease the intra-abdominal pressure (IAP) of SAP patients and have preventive and therapeutic effects on abdominal compartment syndrome of SAP. 展开更多
关键词 Da cheng Qi Decoction Glauber's salt traditional Chinese medicine severe acute pancreatitis Abdominal compartment syndrome
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Relationship of Parental Indica-Japonica Indexes with Yield and Grain Quality Traits of Japonica Hybrid Rice in Northern China 被引量:1
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作者 WANG Yan-rong QIu Fu-lin HUA Ze-tian DAI Gui-jin 《Rice science》 SCIE 2010年第3期199-205,共7页
Taking the main parents (10 male sterile lines and 10 restorer lines) and their 100 combinations of japonica hybrid rice in northern China as materials, the relationships of parental indica-japonica indexes determin... Taking the main parents (10 male sterile lines and 10 restorer lines) and their 100 combinations of japonica hybrid rice in northern China as materials, the relationships of parental indica-japonica indexes determined by the methods of the Cheng’s index as well as simple sequence repeat (SSR) markers with yield and grain quality traits of hybrid rice were studied. For the parents, the Cheng’s index (Chi) ranged from 13.5 to 19.3 and the indica index in SSR markers (ADi) were from 0.12 to 0.38. The classification of parents by Chi was not completely consistent with that by ADi. The Chi of male parent was more closely related to hybrid traits than that of female parent, as contrasted to ADi. At the same time, the difference between parents (PD) in Chi was more closely related to hybrid traits than that in ADi. The indica-japonica indexes of parents and their difference between parents appeared quadratic relationship to hybrid traits with the critical extremum. The directions of the correlation of indica-japonica indexes of parents and their differences with hybrid yield traits were on the opposition to those with hybrid grain quality traits. Therefore, the female parent should match the male parent moderately in indica-japonica index to obtain the optimum of hybrid traits, high yield as well as good quality. 展开更多
关键词 japonica hybrid rice indica-japonica index chengs index simple sequence repeat YIELD rice quality
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The Relation between Genetic Difference for Parents of Hybrid Rice and Heterosis 被引量:1
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作者 Xudong Zhu Xieli Tong +3 位作者 Ju Zhao Ziliang Zhu Dan Zhu Qingming Zhou 《Agricultural Sciences》 CAS 2023年第4期485-498,共14页
Exploring the indica-japonica differentiation in parents of hybridization can provide theoretical bases for utilizing inter-subspecific heterosis. In this study, 5 sterile lines and 18 self-bred restorer lines were us... Exploring the indica-japonica differentiation in parents of hybridization can provide theoretical bases for utilizing inter-subspecific heterosis. In this study, 5 sterile lines and 18 self-bred restorer lines were used as female parents and male parents respectively. Then 90 combinations were constructed by incomplete diallel cross followed by relationship analysis between parental Cheng’s index difference value and Euclidean distance and heterosis. The results showed a significant correlation between several phenotype values, super male parent heterosis and control heterosis and Euclidean distance or Cheng’s index difference value. However, it was no significant correlation for yield. Further analysis found a common interval, 3.41 - 3.46 for Euclidean distance and 3 - 4 for cheng’s index difference value of parents, which was significant or high significant positive correlated with phenotype value, super male parent and control heterosis of main yield traits. This illustrates that the larger the genetic difference of parents was, the stronger the heterosis combinations were, when the genetic differences of parents were in an appropriate range. 展开更多
关键词 Indica and Japonica subspecies HETEROsIs chengs Index Euclidean Distance
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Comparison of Morphological and Genetic Differentiations in Filial Generation of Cross Between Indica and Japonica Rice
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作者 MAO Ting XU Hai Xu Quan Guo Yan-hua ZHU Chun-jie CHEN Kai WANG Jia-yu Xu Zheng-jin 《Rice science》 SCIE 2010年第1期82-86,共5页
A recombinant inbred line (RIL) population of F8 and F9 generations derived from a cross between a typical indica rice (Qishanzhan) and a typical japonica rice (Akihikari) was used to study the difference betwee... A recombinant inbred line (RIL) population of F8 and F9 generations derived from a cross between a typical indica rice (Qishanzhan) and a typical japonica rice (Akihikari) was used to study the difference between morphological differentiation based on phenotype characters and genetic differentiation using indica and japonica specific SSR markers, and to evaluate the relationship between vascular bundle characters and morphological and genetic differentiations. The results showed that the frequency distributions of morphological and genetic differentiations were all inclined to japonica type in the filial generation. The population was more inclined to japonica type based on genetic differentiation than on morphological differentiation. The consistent degrees of classification based on the Cheng’s index, the ratio of large vascular bundle number to small vascular bundle number in panicle neck (RLSVB) and the ratio of large vascular bundle number in the second internode from the top to that in the panicle neck (RLVB) were all about 50% compared with the genetic differentiation, and the consistent degree of the total scores of the Cheng’s index combined with the vascular bundle number ratios was significantly increased to about 80% compared with the genetic differentiation. Therefore, the vascular bundle characters could be used as a helpful supplement for subspecies classification. 展开更多
关键词 rice recombinant inbred line cheng's index vascular bundle characters simple sequence repeat marker subspecies classification method
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程为平教授从伏毒致病角度探讨肌萎缩侧索硬化症辨治思路管窥
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作者 刘鹏 高国杰 +1 位作者 李航 吴迪 《中医药学报》 CAS 2024年第12期52-55,共4页
程为平教授指出,肌萎缩侧索硬化症病邪始萌,症状隐匿,伏而不觉,迁延尔后,发病则病势暴戾,凶险骤显,颇类伏毒。其伏毒可禀自天资,亦可自外袭及内孳,毒伏于体,潜而不发,暗消气血精津液,损及脏腑、脑及髓,毒邪积聚酝酿,伺机而动,一旦发病,... 程为平教授指出,肌萎缩侧索硬化症病邪始萌,症状隐匿,伏而不觉,迁延尔后,发病则病势暴戾,凶险骤显,颇类伏毒。其伏毒可禀自天资,亦可自外袭及内孳,毒伏于体,潜而不发,暗消气血精津液,损及脏腑、脑及髓,毒邪积聚酝酿,伺机而动,一旦发病,则症状杂合,病来急暴。其病机可概括为毒潜脏腑、损督及脊、毒伏脑髓、毁损元神两端,核心治则可概括为扶正祛毒,通督填髓。程为平教授临证每针药结合,尤善针刺,创新针法,颇重颈项。并附验案一则,以资佐证。 展开更多
关键词 肌萎缩侧索硬化症 伏毒 程氏项针
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北大汉简《揕舆》与伶州鸠所言武王伐纣天象
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作者 王宁 《枣庄学院学报》 2024年第6期82-88,共7页
《国语·周语下》记载伶州鸠言武王伐纣天象历来为研究商周史者所重视,据之推测年代者甚众。而根据马王堆汉墓帛书《阴阳五行甲篇·堪舆》和北大汉简《揕舆》记载的先秦堪舆术看,《左传》《国语》中以天文作占卜预言以及言十二... 《国语·周语下》记载伶州鸠言武王伐纣天象历来为研究商周史者所重视,据之推测年代者甚众。而根据马王堆汉墓帛书《阴阳五行甲篇·堪舆》和北大汉简《揕舆》记载的先秦堪舆术看,《左传》《国语》中以天文作占卜预言以及言十二次的内容都与堪舆术有关,而并非真实的天象,其中凡与十二次名有关者多为汉代人所改窜,均不能用来作为推测年份的依据,伶州鸠之语即属此类。 展开更多
关键词 伶州鸠 武王伐纣 天象 堪舆 十二次
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中医治疗肾性水肿的临床思路
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作者 江理芳 张春戬 《河南中医》 2024年第11期1665-1669,共5页
肾性水肿的发病主要与脏腑功能失司密切相关,尤以肺、脾、肾三脏为主,究其根本,主要病机为本虚标实。肺主宣发肃降,脾主运化水液,肾主温煦化饮,三脏功能相互为用,是维持机体内水液正常运行的重要条件。《素问·汤液醪醴论》中记载... 肾性水肿的发病主要与脏腑功能失司密切相关,尤以肺、脾、肾三脏为主,究其根本,主要病机为本虚标实。肺主宣发肃降,脾主运化水液,肾主温煦化饮,三脏功能相互为用,是维持机体内水液正常运行的重要条件。《素问·汤液醪醴论》中记载了治疗水肿的基本治法——“开鬼门”“洁净府”“去宛陈莝”,即宣通肺气、通腑泄浊、活血化瘀以利水消肿。三法之中“洁净府”最为关键,贯穿疾病始终,发挥好其在“开鬼门”与“去宛陈莝”之间的枢纽作用,在辨证论治的基础上通过温阳化气、益气养阴、利水消肿等“洁净府”的治疗方法达到“祛邪而不伤正,扶正而不助邪”的目的。然三法各有所宜,当抓住主要病机,调养受损脏腑,清除邪气,以维持气血津液正常运行和脏腑功能的正常运转。 展开更多
关键词 “开鬼门” “洁净府” “去宛陈莝” 肾性水肿 《黄帝内经》
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宣恩县利川润楠种群及群落特征研究 被引量:5
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作者 于鹏宇 吴月淼 +2 位作者 周存宇 胡蝶 周明芹 《湖北农业科学》 2017年第6期1085-1089,共5页
利用生态学方法,对湖北省宣恩县的利川润楠(Machilus lichuanensis Cheng ex S.Lee)天然种群和群落特征进行调查分析。结果表明,宣恩县的利川润楠群落物种丰富,有维管束植物101种,隶属于51科90属;利川润楠种群数量虽多,但结构不合理,处... 利用生态学方法,对湖北省宣恩县的利川润楠(Machilus lichuanensis Cheng ex S.Lee)天然种群和群落特征进行调查分析。结果表明,宣恩县的利川润楠群落物种丰富,有维管束植物101种,隶属于51科90属;利川润楠种群数量虽多,但结构不合理,处于更新层的幼苗和幼树总体上所占比例较小,且在各样地分布不匀;调查范围内的利川润楠种群均缺少足够的演替层;种群对坡度、坡位、坡向、海拔等生态因子具有选择性,海拔700~1 050 m的山地下坡位、阴坡坡向更适合利川润楠的生长。根据调查结果,进一步分析了利川润楠的濒危原因,并提出了相应的保育对策。 展开更多
关键词 利川润楠(Machilus lichuanensis cheng EX s.Lee) 群落分析 种群特征 宣恩县
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黄褐毛忍冬花蕾咖啡酰奎宁酸类成分研究 被引量:29
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作者 汤丹 李会军 +2 位作者 钱正明 毕志明 李萍 《中国药学杂志》 CAS CSCD 北大核心 2007年第20期1537-1539,共3页
目的研究黄褐毛忍冬(Lonicera fulvotomentosa Hsuet S.C.Cheng)花蕾的化学成分,为金银花类药材综合质量评价提供科学依据。方法采用各种色谱技术进行分离纯化,通过化合物理化性质及波谱数据鉴定结构。结果分离鉴定了5个化合物:灰毡毛... 目的研究黄褐毛忍冬(Lonicera fulvotomentosa Hsuet S.C.Cheng)花蕾的化学成分,为金银花类药材综合质量评价提供科学依据。方法采用各种色谱技术进行分离纯化,通过化合物理化性质及波谱数据鉴定结构。结果分离鉴定了5个化合物:灰毡毛素F(Ⅰ),4,5-O-双咖啡酰奎宁酸甲酯(Ⅱ),3,4-O-双咖啡酰奎宁酸(Ⅲ),4,5-O-双咖啡酰奎宁酸(Ⅳ),绿原酸(Ⅴ)。结论以上5种咖啡酰奎宁酸类成分均为首次从该种植物中分离得到。 展开更多
关键词 黄褐毛忍冬 金银花 咖啡酰奎宁酸类
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黄褐毛忍冬不同炮制品中绿原酸的HPLC法含量测定 被引量:14
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作者 张艳焱 张天伦 +2 位作者 梁光义 曹佩雪 阮婧华 《中草药》 CAS CSCD 北大核心 2003年第8期696-697,共2页
目的 比较黄褐毛忍冬花蕾、白花、黄花生晒品及蒸晒品中绿原酸的含量。方法 采用 HPLC法 ,反相 Hy-persil C1 8柱 ,以乙腈 -0 .4%磷酸溶液 (13∶ 87)为流动相 ,检测波长为 3 2 7nm。结果 黄褐毛忍冬花蕾、白花、黄花生晒品绿原酸含... 目的 比较黄褐毛忍冬花蕾、白花、黄花生晒品及蒸晒品中绿原酸的含量。方法 采用 HPLC法 ,反相 Hy-persil C1 8柱 ,以乙腈 -0 .4%磷酸溶液 (13∶ 87)为流动相 ,检测波长为 3 2 7nm。结果 黄褐毛忍冬花蕾、白花、黄花生晒品绿原酸含量分别为 0 .5 4% ,0 .81% ,0 .5 7% ;蒸晒品中绿原酸含量分别为 2 .14 % ,1.65 % ,2 .0 4%。 展开更多
关键词 黄褐毛忍冬 炮制 绿原酸 高效液相色谱
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薄层扫描法测定金银花中黄褐毛忍冬皂苷甲的含量 被引量:7
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作者 贾宪生 吴洪飞 茅青 《药物分析杂志》 CAS CSCD 北大核心 2005年第6期719-721,共3页
目的:采用薄层扫描法测定金银花中黄褐毛忍冬皂苷甲的含量。方法:硅胶 G 薄层板,展开剂:氯仿-甲醇-水(61∶32∶5),显色:10%硫酸乙醇溶液喷雾,105℃烘烤约5min,双波长锯齿扫描,检测波长518nm,参比波长700nm。结果:黄褐毛忍冬皂苷甲的线... 目的:采用薄层扫描法测定金银花中黄褐毛忍冬皂苷甲的含量。方法:硅胶 G 薄层板,展开剂:氯仿-甲醇-水(61∶32∶5),显色:10%硫酸乙醇溶液喷雾,105℃烘烤约5min,双波长锯齿扫描,检测波长518nm,参比波长700nm。结果:黄褐毛忍冬皂苷甲的线性范围为1.20~7.20μg,r=0.998。平均回收率为99.76%,RSD 为2.45%。结论:用于金银花中皂苷类成分的分析,本法简便、快速,重现性好。 展开更多
关键词 黄褐毛忍冬 薄层扫描法 皂苷甲 金银花 含量 测定 硫酸乙醇溶液 平均回收率 皂苷类成分 检测波长 线性范围 薄层板 硅胶G 展开剂 双波长 RsD 重现性
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霍山石斛及相似种的位点特异性PCR鉴别 被引量:18
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作者 刘石泉 李小军 +1 位作者 余庆波 周根余 《中草药》 CAS CSCD 北大核心 2006年第1期111-115,共5页
目的设计出霍山石斛的位点特异性鉴别引物,仅通过PCR就能对霍山石斛的真伪进行准确鉴别。方法利用石斛属植物基因库中rDNA ITS序列数据库进行同源性比较,在与霍山石斛差异较大的区段设计1对特异性引物,然后对19份石斛属植物DNA模板进行... 目的设计出霍山石斛的位点特异性鉴别引物,仅通过PCR就能对霍山石斛的真伪进行准确鉴别。方法利用石斛属植物基因库中rDNA ITS序列数据库进行同源性比较,在与霍山石斛差异较大的区段设计1对特异性引物,然后对19份石斛属植物DNA模板进行PCR扩增,阳性者即为霍山石斛正品。结果发现在退火温度为60℃时,该引物能把霍山石斛的模板从19份石斛属植物中扩增出来,而其他的石斛属植物均为阴性。结论运用位点特异性鉴别引物成功地对霍山石斛进行PCR鉴别,该鉴别反应重现性好,能在霍山石斛鉴别时发挥重要的作用,与形态学、DNA测序等鉴别方法相比,该方法具有高效、准确、简便、省时等优点。 展开更多
关键词 霍山石斛 位点特异性PCR 鉴别
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霍山石斛生理生化性质的研究进展 被引量:25
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作者 吕素芳 郭广君 蔡永萍 《中草药》 CAS CSCD 北大核心 2006年第5期790-793,共4页
石斛属(D end robium Sw.)为兰科植物,约有1 500种。我国有80种,其中药用石斛有51种。在我国传统医学中,石斛为常用药材,现代药理研究证明石斛具有抗肿瘤、抗衰老、降血糖等作用。霍山石斛是安徽省特产的药用石斛,为石斛中的上品。综述... 石斛属(D end robium Sw.)为兰科植物,约有1 500种。我国有80种,其中药用石斛有51种。在我国传统医学中,石斛为常用药材,现代药理研究证明石斛具有抗肿瘤、抗衰老、降血糖等作用。霍山石斛是安徽省特产的药用石斛,为石斛中的上品。综述了霍山石斛的生理生化、生物学特性、有效成分等方面的研究现状。 展开更多
关键词 石斛属 霍山石斛 生理生化 生物学特性
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